Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 68, Part 6 (June 2012)



[Issue Author Index][Volume Author Index]
[Cover illustration] Cover illustration: Structure of a Staphylococcus aureus 3-methyladenine DNA glycosylase I complex (Zhu et al., p. 610).

structural communications


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Acta Cryst. (2012). F68, 610-615  [ doi:10.1107/S1744309112016363 ]

A model for 3-methyladenine recognition by 3-methyladenine DNA glycosylase I (TAG) from Staphylococcus aureus

X. Zhu, X. Yan, L. G. Carter, H. Liu, S. Graham, P. J. Coote and J. Naismith

Synopsis: The structure of 3-methyladenine DNA glycosylase I in complex with 3-methyladenine is reported.

PDB references: 4aia and 4ai5

Online 22 May 2012


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Acta Cryst. (2012). F68, 616-621  [ doi:10.1107/S1744309112015618 ]

Structure of human ADAM-8 catalytic domain complexed with batimastat

T. Hall, H.-S. Shieh, J. E. Day, N. Caspers, J. E. Chrencik, J. M. Williams, L. E. Pegg, A. M. Pauley, A. F. Moon, J. M. Krahn, D. H. Fischer, J. R. Kiefer, A. G. Tomasselli and M. D. Zack

Synopsis: The catalytic domain of human ADAM-8 was expressed, purified and crystallized in complex with a hydroxamic acid inhibitor, batimastat. The crystal structure of the enzyme-inhibitor complex was refined to 2.1 Å resolution.

PDB reference: 4dd8

Online 22 May 2012


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Acta Cryst. (2012). F68, 622-625  [ doi:10.1107/S1744309112017435 ]

Structure of a monomeric variant of rhodopsin kinase at 2.5 Å resolution

J. J. G. Tesmer, M. R. Nance, P. Singh and H. Lee

Synopsis: The structure of the L166K variant of G protein-coupled receptor kinase 1 has been determined at 2.5 Å resolution in order to determine how a dimer interface observed in prior crystal structures influences the conformation of the enzyme and how the C-terminal amino acids are configured while in a monomeric state.

PDB reference: 3t8o

Online 22 May 2012


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Acta Cryst. (2012). F68, 626-631  [ doi:10.1107/S1744309112013541 ]

Structure of the GDP-bound G domain of the RGK protein Rem2

P. Reymond, A. Coquard, M. Chenon, M. Zeghouf, A. El Marjou, A. Thompson and J. Ménétrey

Synopsis: The structure of the Rem2 G domain bound to GDP is reported in a monoclinic crystal form at 2.66 Å resolution.

PDB reference: 4aii

Online 22 May 2012


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Acta Cryst. (2012). F68, 632-637  [ doi:10.1107/S1744309112017939 ]

Structure of a short-chain dehydrogenase/reductase from Bacillus anthracis

J. Hou, K. Wojciechowska, H. Zheng, M. Chruszcz, D. R. Cooper, M. Cymborowski, T. Skarina, E. Gordon, H. Luo, A. Savchenko and W. Minor

Synopsis: The crystal structure of a short-chain dehydrogenase/reductase from B. anthracis strain `Ames Ancestor' is reported.

PDB reference: 3icc

Online 24 May 2012


crystallization communications


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Acta Cryst. (2012). F68, 638-643  [ doi:10.1107/S1744309112011062 ]

Expression, purification and preliminary structural analysis of Escherichia coli MatP in complex with the matS DNA site

D. Durand, I. Li de la Sierra-Gallay, M. A. Brooks, A. W. Thompson, N. Lazar, J. Lisboa, H. van Tilbeurgh and S. Quevillon-Cheruel

Synopsis: MatP is a small DNA-binding protein of about 18 kDa. In order to understand the DNA-compaction mechanism of MatP at an atomic level, the structures of apo MatP and of the nucleoprotein complex MatP-matS have been studied.

Online 22 May 2012


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Acta Cryst. (2012). F68, 644-646  [ doi:10.1107/S1744309112013243 ]

Crystallization and preliminary crystallographic analysis of D-aspartate oxidase from porcine kidney

M. Senda, A. Yamamoto, H. Tanaka, T. Ishida, K. Horiike and T. Senda

Synopsis: D-Aspartate oxidase from porcine kidney was crystallized by the sitting-drop vapour-diffusion method. The crystal diffracted to 1.80 Å resolution.

Online 22 May 2012


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Acta Cryst. (2012). F68, 647-651  [ doi:10.1107/S1744309112013267 ]

Crystallization and preliminary X-ray crystallographic analysis of the Bag2 amino-terminal domain from Mus musculus

R. C. Page, Z. Xu, J. Amick, J. C. Nix and S. Misra

Synopsis: The amino-terminal domain of the Hsp70 co-chaperone Bag2 from M. musculus has been crystallized in native and selenomethionyl forms diffracting to 2.27 and 3.1 Å resolution, respectively.

Online 23 May 2012


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Acta Cryst. (2012). F68, 652-654  [ doi:10.1107/S1744309112013942 ]

Crystallization and preliminary X-ray analysis of the haloalkane dehalogenase DatA from Agrobacterium tumefaciens C58

T. Mase, H. Yabuki, M. Okai, J. Ohtsuka, F. L. Imai, Y. Nagata and M. Tanokura

Synopsis: The haloalkane dehalogenase DatA from A. tumefaciens C58 was expressed, purified and crystallized by the sitting-drop vapour-diffusion method. X-ray diffraction data were collected to 1.70 Å resolution.

Online 23 May 2012


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Acta Cryst. (2012). F68, 655-658  [ doi:10.1107/S1744309112013954 ]

Purification, crystallization and preliminary X-ray crystallographic analysis of the ATPase domain of human TAP in nucleotide-free and ADP-, vanadate- and azide-complexed forms

S. R. Meena, S. P. Gangwar and A. K. Saxena

Synopsis: The ATPase domain of human TAP in nucleotide free, ADP, vanadate and azide complexed forms were purified and crystallized. The X-ray diffraction data sets were collected for all crystals in the resolution range of 2.8-3.0 Å.

Online 23 May 2012


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Acta Cryst. (2012). F68, 659-662  [ doi:10.1107/S1744309112014571 ]

Crystallization and preliminary X-ray diffraction of malate dehydrogenase from Plasmodium falciparum

C. Wrenger, I. B. Müller, S. Butzloff, R. Jordanova, S. Lunev and M. R. Groves

Synopsis: The collection of X-ray diffraction data from malate dehydrogenase from P. falciparum to a resolution of 2.2 Å is reported.

Online 23 May 2012


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Acta Cryst. (2012). F68, 663-667  [ doi:10.1107/S1744309112014662 ]

Crystallization and preliminary crystallographic studies of both components of the staphylococcal LukE-LukD leukotoxin

R. Galy, F. Bergeret, D. Keller, L. Mourey, G. Prévost and L. Maveyraud

Synopsis: The expression, purification and crystallization of the soluble forms of LukE and LukD, which together constitute the LukE-LukD staphylococcal leukotoxin, are described.

Online 23 May 2012


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Acta Cryst. (2012). F68, 668-670  [ doi:10.1107/S1744309112015333 ]

Crystallization and preliminary X-ray crystallographic analysis of human peptidylarginine deiminase type III

M. Unno, K. Kizawa, M. Ishihara and H. Takahara

Synopsis: Human peptidylarginine deiminase type III was crystallized using polyethylene glycol monomethylether or polyethylene glycol as a precipitant. The crystals belonged to space group R3, with unit-cell parameters a = b = 114.97, c = 332.49 Å (hexagonal axes), and contained two molecules in an asymmetric unit.

Online 23 May 2012


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Acta Cryst. (2012). F68, 671-674  [ doi:10.1107/S1744309112015862 ]

Expression, purification, crystallization and preliminary X-ray analysis of Plasmodium falciparum GTP:AMP phosphotransferase

A. W. L. Law, J. Lescar, Q. Hao and M. Kotaka

Synopsis: P. falciparum GTP:AMP phosphotransferase was expressed in E. coli, purified and crystallized. An X-ray diffraction data set was collected to a resolution of 2.90 Å.

Online 23 May 2012


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Acta Cryst. (2012). F68, 675-677  [ doi:10.1107/S1744309112015692 ]

Purification, crystallization and preliminary X-ray analysis of human histidine decarboxylase

H. Komori, Y. Nitta, H. Ueno and Y. Higuchi

Synopsis: Human histidine decarboxylase was crystallized by the sitting-drop vapour-diffusion method. Diffraction data were collected to 1.8 Å resolution.

Online 23 May 2012


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Acta Cryst. (2012). F68, 678-682  [ doi:10.1107/S1744309112016004 ]

Crystallization and preliminary X-ray crystallographic analysis of bacterial tRNASec in complex with seryl-tRNA synthetase

Y. Itoh, S. Sekine and S. Yokoyama

Synopsis: Bacterial selenocysteine tRNA was crystallized as the heterologous complex with archaeal seryl-tRNA synthetase. X-ray diffraction was improved by introducing point mutations and heavy-atom labeling, and a 3.2 Å diffraction data set for phase determination was obtained from a platinum-labeled crystal.

Online 23 May 2012


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Acta Cryst. (2012). F68, 683-686  [ doi:10.1107/S1744309112016181 ]

Crystallization and preliminary X-ray analysis of the C-terminal domain of CCM2, part of a novel adaptor protein involved in cerebral cavernous malformations

X. Wang, J. Ding and D. Wang

Synopsis: The purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal domain of CCM2, which is part of a signalling complex, are reported.

Online 23 May 2012


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Acta Cryst. (2012). F68, 687-691  [ doi:10.1107/S174430911201665X ]

Overexpression, purification, crystallization and preliminary X-ray analysis of putative molybdenum cofactor biosynthesis protein C (MoaC2) from Mycobacterium tuberculosis H37Rv

S. Srivastava, V. K. Srivastava, A. Arora and J. V. Pratap

Synopsis: Rv0864, an enzyme of the Moco biosynthesis pathway from M. tuberculosis, has been overexpressed, purified and crystallized. Diffraction data to 2.5 Å were collected and the structure solved by molecular replacement.

Online 23 May 2012


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Acta Cryst. (2012). F68, 692-694  [ doi:10.1107/S1744309112016569 ]

Cloning, purification, crystallization and preliminary X-ray studies of human [alpha]1-microglobulin

Y. Zhang, Z. Gao, Z. Zhang, M. Luo, A. Huang, Y. Dong and D. Wang

Synopsis: Here, the expression, purification, crystallization and preliminary crystallographic analysis of human [alpha]1m are reported.

Online 23 May 2012


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Acta Cryst. (2012). F68, 695-697  [ doi:10.1107/S1744309112016661 ]

Purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal fragment of the MvfR protein from Pseudomonas aeruginosa

K. Kefala, D. Kotsifaki, M. Providaki, E. G. Kapetaniou, L. Rahme and M. Kokkinidis

Synopsis: MvfRC87, a 242-residue C-terminal segment of the LysR-type transcriptional regulator MvfR, was produced in Escherichia coli, purified and crystallized.

Online 23 May 2012


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Acta Cryst. (2012). F68, 698-700  [ doi:10.1107/S1744309112017137 ]

Crystallization of the novel S-adenosyl-L-methionine-dependent C-methyltransferase CouO from Streptomyces rishiriensis and preliminary diffraction data analysis

A. Lyskowski, M. Tengg, G. Steinkellner, H. Schwab, M. Gruber-Khadjawi and K. Gruber

Synopsis: Recombinant Q9F8T9 protein from Streptomyces rishiriensis (CouO), an S-adenosyl-L-methionine-dependent C-methyltransferase, has been successfully cloned, expressed and purified.

Online 23 May 2012


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Acta Cryst. (2012). F68, 701-704  [ doi:10.1107/S1744309112017307 ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the apo form of InsP5 2-K from Arabidopsis thaliana

J. I. Baños-Sanz, J. Sanz-Aparicio, C. A. Brearley and B. González

Synopsis: A mutated version of InsP5 2-K allows the production of crystals of the apo form and structure determination using X-ray crystallography.

Online 23 May 2012


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Acta Cryst. (2012). F68, 705-708  [ doi:10.1107/S1744309112017915 ]

Purification, crystallization and preliminary X-ray diffraction data of UDP-galactopyranose mutase from Aspergillus fumigatus

G. A. Penman, D. E. A. Lockhart, A. Ferenbach and D. M. F. van Aalten

Synopsis: The cloning, overexpression, purification, crystallization and preliminary X-ray diffraction data are described for UDP-galactopyranose mutase, an enzyme involved in cell-wall synthesis in A. fumigatus.

Online 23 May 2012


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Acta Cryst. (2012). F68, 709-712  [ doi:10.1107/S1744309112018131 ]

Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of a cyclophilin A-like protein from Piriformospora indica

H. Bhatt, D. K. Trivedi, R. K. Pal, A. K. Johri, N. Tuteja and N. S. Bhavesh

Synopsis: Cyclophin A like protein from Piriformospora indica involved in salt-stress tolerance was cloned, overexpressed, purified and crystallized. The crystals obtained diffracted X-rays to 2 Å resolution and belonged to space group C2221.

Online 24 May 2012


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Acta Cryst. (2012). F68, 713-715  [ doi:10.1107/S174430911201826X ]

Crystallization and preliminary X-ray crystallographic analysis of Thermotoga maritima CheA P3-P4-P5 domains in complex with CheW

S. Park, K. Y. Kim, S. Kim and B. R. Crane

Synopsis: T. maritima CheA P3-P4-P5 domains were crystallized in complex with CheW. Low-resolution diffraction data were collected to ~8 Å using synchrotron X-ray radiation.

Online 24 May 2012


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Acta Cryst. (2012). F68, 716-719  [ doi:10.1107/S1744309112018623 ]

Purification, crystallization and preliminary X-ray analysis of nonstructural protein 2 (nsp2) from avian infectious bronchitis virus

K. Yu, Z. Ming, Y. Li, C. Chen, Z. Bao, Z. Ren, B. Liu, W. Tao, Z. Rao and Z. Lou

Synopsis: Nonstructural protein 2 from avian infectious bronchitis virus has been overexpressed in E. coli, purified and crystallized. Diffraction data were collected to 2.8 Å resolution.

Online 24 May 2012


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Acta Cryst. (2012). F68, 720-723  [ doi:10.1107/S1744309112016909 ]

Crystallization and preliminary X-ray analysis of the reductase component of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii

W. Oonanant, J. Sucharitakul, P. Chaiyen and J. Yuvaniyama

Synopsis: The reductase component of p-hydroxyphenylacetate 3-hydroxylase from A. baumannii was overexpressed, purified and crystallized. X-ray diffraction data were collected and processed to 2.3 Å resolution.

Online 24 May 2012


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Acta Cryst. (2012). F68, 724-729  [ doi:10.1107/S1744309112015230 ]

The ParE2-PaaA2 toxin-antitoxin complex from Escherichia coli O157 forms a heterodocecamer in solution and in the crystal

Y. G. J. Sterckx, A. Garcia-Pino, S. Haesaerts, T. Jové, L. Geerts, V. Sakellaris, L. Van Melderen and R. Loris

Synopsis: Crystals of the complex of E. coli O157 ParE2 and PaaA2 belong to space group P3121 or P3221 and diffract to 3.8 Å resolution.

Online 25 May 2012


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