Structural Biology and Crystallization Communications

 

Acta Cryst. (2012). F68, 730-737  [ doi:10.1107/S1744309112010603 ]

Structure of the regulatory domain of the LysR family regulator NMB2055 (MetR-like protein) from Neisseria meningitidis

S. Sainsbury, J. Ren, N. J. Saunders, D. I. Stuart and R. J. Owens

Synopsis: The crystal structure of the regulatory domain of NMB2055, a putative MetR regulator, was solved at 2.5 Å resolution.

PDB references: 4ab5 and 4ab6

Online 22 June 2012

 

Acta Cryst. (2012). F68, 738-743  [ doi:10.1107/S1744309112019124 ]

Structure of Leishmania major cysteine synthase

P. K. Fyfe, G. D. Westrop, T. Ramos, S. Müller, G. H. Coombs and W. N. Hunter

Synopsis: A crystallographic and biochemical study of L. major cysteine synthase, which is a pyridoxyl phosphate-dependent enzyme, is reported. The structure was determined to 1.8 Å resolution and revealed that the cofactor has been lost and that a fragment of -poly-D-glutamic acid, a crystallization ingredient, was bound in the active site. The enzyme was inhibited by peptides.

PDB reference: 4air

Online 22 June 2012

 

Acta Cryst. (2012). F68, 744-750  [ doi:10.1107/S1744309112020167 ]

Structure of the signal transduction protein TRAP (target of RNAIII-activating protein)

K. Henrick and M. Hirshberg

Synopsis: The 1.85 Å resolution structure of the signal transduction protein TRAP is presented. The overall fold of TRAP is an unsymmetrical eight-stranded -barrel with five helices.

PDB reference: 4ae5

Online 22 June 2012

 

Acta Cryst. (2012). F68, 751-756  [ doi:10.1107/S1744309112020635 ]

Structure of the RBD-PRDI fragment of the antiterminator protein GlcT

S. Himmel, C. Grosse, S. Wolff, C. Schwiegk and S. Becker

Synopsis: The crystal structure of the RBD-PRDI fragment of the antiterminator protein GlcT from Bacillus subtilis has been solved at 2 Å resolution. The structure represents an inactive state of the protein.

PDB reference: 3rio

Online 22 June 2012

 

Acta Cryst. (2012). F68, 757-759  [ doi:10.1107/S1744309112018969 ]

Crystallization and preliminary X-ray crystallographic analysis of Pz peptidase B from Geobacillus collagenovorans MO-1

H. Nakano, A. Hosokawa, R. Tagawa, K. Inaka, K. Ohta, T. Nakatsu, H. Kato and K. Watanabe

Synopsis: In this work, Pz peptidase B, an intracellular M3 metallopeptidase that is found in the thermophile Geobacillus collagenovorans MO-1, was crystallized using the counter-diffusion method.

Online 27 June 2012

 

Acta Cryst. (2012). F68, 760-763  [ doi:10.1107/S1744309112016843 ]

Crystallization and preliminary crystallographic studies of CCM3 in complex with the C-terminal domain of MST4

X. Xu, X. Wang, J. Ding and D.-C. Wang

Synopsis: The complex of CCM3 and the C-terminal domain of MST4 has been successfully constructed, purified and crystallized. The crystal diffracted to a resolution of 2.4 Å.

Online 27 June 2012

 

Acta Cryst. (2012). F68, 764-766  [ doi:10.1107/S174430911201740X ]

Purification, crystallization and preliminary crystallographic analysis of histone lysine demethylase NO66 from Homo sapiens

X. Zhou, Y. Tao, M. Wu, D. Zhang and J. Zang

Synopsis: The JmjC domain-containing histone demethylase NO66 from H. sapiens was overproduced in E. coli, purified and crystallized. Diffraction data were collected to 2.29 Å resolution.

Online 27 June 2012

 

Acta Cryst. (2012). F68, 767-770  [ doi:10.1107/S1744309112017812 ]

Crystallization and preliminary X-ray analysis of a novel halotolerant feruloyl esterase identified from a soil metagenomic library

S. Chen, K. Wang, Y. Liu and X. Hu

Synopsis: A novel feruloyl esterase (EstF27) identified from a soil metagenomic library has been crystallized and a complete data set was collected from a single cooled crystal using an in-house X-ray source.

Online 27 June 2012

 

Acta Cryst. (2012). F68, 771-774  [ doi:10.1107/S1744309112018672 ]

Crystallization of an atypical short-chain dehydrogenase from Vibrio vulnificus lacking the conserved catalytic tetrad

G. Buysschaert, K. Verstraete, S. N. Savvides and B. Vergauwen

Synopsis: An atypical short-chain dehydrogenase/reductase from Vibrio vulnificus was expressed, purified and cocrystallized with NADPH by the sitting-drop vapour-diffusion method. X-ray diffraction data were collected to 1.80 Å.

Online 27 June 2012

 

Acta Cryst. (2012). F68, 775-777  [ doi:10.1107/S1744309112019276 ]

Cloning, purification, crystallization and preliminary X-ray diffraction crystallographic study of acyl-protein thioesterase 1 from Saccharomyces cerevisiae

Y. Yuan, X. Wang, X. Li, M. Teng, L. Niu and Y. Gao

Synopsis: This paper reports the cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of acyl-protein thioesterase 1 from S. cerevisiae.

Online 27 June 2012

 

Acta Cryst. (2012). F68, 778-782  [ doi:10.1107/S1744309112019306 ]

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp1

K. Kato, H. Nishimasu, E. Mihara, R. Ishitani, J. Takagi, J. Aoki and O. Nureki

Synopsis: The extracellular region of mouse Enpp1 was expressed, purified and crystallized. An X-ray diffraction data set was collected to 3.0 Å resolution by employing a helical data-collection strategy involving a micro-focus synchrotron beam.

Online 27 June 2012

 

Acta Cryst. (2012). F68, 783-785  [ doi:10.1107/S1744309112020180 ]

Crystallization and X-ray diffraction studies of arginine kinase from the white Pacific shrimp Litopenaeus vannamei

A. A. Lopez-Zavala, R. R. Sotelo-Mundo, K. D. Garcia-Orozco, F. Isac-Martinez, L. G. Brieba and E. Rudiño-Piñera

Synopsis: The crystallization and preliminary X-ray diffraction analysis at 1.25 Å resolution of free-ligand arginine kinase from the Pacific whiteleg shrimp Litopenaeus vannamei are reported. Crystals belong to space group P2₁2₁2₁, phases were determined by molecular replacement and refinement was performed with Phenix.

Online 27 June 2012

    

Acta Cryst. (2012). F68, 786-789  [ doi:10.1107/S1744309112020301 ]

Crystallization and preliminary X-ray diffraction analysis of the high molecular weight ketoacyl reductase FabG4 complexed with NADH

D. Dutta, S. Bhattacharyya and A. K. Das

Synopsis: The cloning, overexpression, purification, crystallization in a triclinic space group and preliminary X-ray diffraction analysis of the high-molecular-weight ketoacyl reductase FabG4 complexed with NADH are reported.

Online 27 June 2012

 

Acta Cryst. (2012). F68, 790-792  [ doi:10.1107/S1744309112020222 ]

Expression, purification, crystallization and preliminary crystallographic analysis of the phosphoglycerate kinase from Acinetobacter baumannii

K. Baretta, C. Garen, J. Yin and M. N. G. James

Synopsis: Approximately five decades have passed with only one or two new antibiotics making it into clinical use. Phosphoglycerate kinase from A. baumanii has been selected as a potential target for antibiotic development; this paper presents the initial structural biological results from this research.

Online 27 June 2012

 

Acta Cryst. (2012). F68, 793-797  [ doi:10.1107/S1744309112020313 ]

Purification, crystallization and preliminary crystallographic analysis of the adhesion domain of Epf from Streptococcus pyogenes

C. Linke, N. Siemens, M. J. Middleditch, B. Kreikemeyer and E. N. Baker

Synopsis: The putative adhesion domain of the multidomain protein Epf from S. pyogenes has been crystallized in space groups P2₁ and P2₁2₁2₁. The crystals diffracted to 2.0 and 1.6 Å resolution, respectively, at the Australian Synchrotron.

Online 28 June 2012

 

Acta Cryst. (2012). F68, 798-801  [ doi:10.1107/S1744309112016351 ]

Crystallization and preliminary X-ray diffraction studies of the GhKCH2 motor domain: alteration of pH significantly improved the quality of the crystals

X. Qin, Z. Chen, T. Xu, P. Li and G. Liu

Synopsis: The GhKCH2 motor domain was crystallized and the pH of the crystallization buffer was shown to have a significant effect on the crystal morphology and diffraction quality.

Online 28 June 2012

 

Acta Cryst. (2012). F68, 802-805  [ doi:10.1107/S1744309112020556 ]

Purification, crystallization and preliminary X-ray diffraction analysis of the effector protein PevD1 from Verticillium dahliae

L. Han, Z. Liu, X. Liu and D. Qiu

Synopsis: The overexpression, purification, crystallization and preliminary X-ray diffraction analysis of protein elicitor PevD1 from Verticillium dahliae are reported.

Online 28 June 2012

    

Acta Cryst. (2012). F68, 806-809  [ doi:10.1107/S1744309112020465 ]

Crystallization and preliminary X-ray crystallographic analysis of an ice-binding protein (FfIBP) from Flavobacterium frigoris PS1

H. Do, J. H. Lee, S. G. Lee and H. J. Kim

Synopsis: The ice-binding protein FfIBP from F. frigoris PS1 was overexpressed, purified, characterized and crystallized, and preliminary X-ray crystallographic analysis was performed.

Online 28 June 2012

 

Acta Cryst. (2012). F68, 810-812  [ doi:10.1107/S1744309112021720 ]

Expression, purification, crystallization and preliminary X-ray analysis of the D-alanyl carrier protein DltC from Staphylococcus epidermidis

C.-H. Huang, C.-H. Kao, C.-S. Yang, C.-H. Chang, S.-C. Chen, S.-M. Kuan, Y.-C. Su, Y.-H. Huang, M.-C. Chang and Y. Chen

Synopsis: The S. epidermidis carrier protein DltC has been crystallized in order to elucidate the functional role of DltC in the alanylation of lipoteichoic acids in bacteria.

Online 28 June 2012

 

Acta Cryst. (2012). F68, 813-815  [ doi:10.1107/S1744309112021744 ]

Expression, purification, crystallization and preliminary X-ray analysis of the human NORE1 SARAH domain

H. J. Kim, E. Hwang, Y.-H. Han, S. Choi, W. C. Lee, H.-Y. Kim, Y. H. Jeon, C. Cheong and H.-K. Cheong

Synopsis: The crystallization of the human NORE1 SARAH domain is reported.

Online 28 June 2012

 

Acta Cryst. (2012). F68, 816-819  [ doi:10.1107/S1744309112022208 ]

Crystallization of SHARPIN using an automated two-dimensional grid screen for optimization

B. Stieglitz, K. Rittinger and L. F. Haire

Synopsis: The expression, purification and crystallization of an N-terminal fragment of SHARPIN are reported. Diffraction-quality crystals were obtained using a two-dimensional grid-screen seeding technique.

Online 28 June 2012

 

Acta Cryst. (2012). F68, 820-823  [ doi:10.1107/S1744309112022488 ]

Purification and crystallization of yeast Ent1 ENTH domain

N. Tanner and G. Prag

Synopsis: The yeast Epsin-1 (ent1) gene has been cloned and expressed in Escherichia coli. The protein product of a construct containing the ENTH-UIM modules was purified to homogeneity and subjected to crystallization screening using the sitting-drop vapour-diffusion method. Refined conditions containing polyethylene glycol 3350 and Tacsimate yielded thin rod-like crystals.

Online 28 June 2012

 

Acta Cryst. (2012). F68, 824-828  [ doi:10.1107/S1744309112019082 ]

Purification, crystallization and preliminary X-ray diffraction analysis of a hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyltransferase (HCT) from Coffea canephora involved in chlorogenic acid biosynthesis

L. A. Lallemand, J. G. McCarthy, S. McSweeney and A. A. McCarthy

Synopsis: A hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyltransferase involved in chlorogenic acid biosynthesis in C. canephora was crystallized using the vapour-diffusion method. A diffraction data set was collected to 3.0 Å resolution on the microfocus beamline (ID23-2) at ESRF and a structure solution was obtained using molecular replacement.

Online 28 June 2012

 

Acta Cryst. (2012). F68, 829-831  [ doi:10.1107/S1744309112022798 ]

Crystallization and preliminary X-ray analysis of the C-terminal domain of -COP, a medium-sized subunit of the COPI complex involved in membrane trafficking

K. Deng, F. Gao, P. Zheng, W. Gong and Z. Sun

Synopsis: The purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal domain of -COP, a medium-sized subunit of the COPI complex, are reported.

Online 28 June 2012

 

Acta Cryst. (2012). F68, 832-834  [ doi:10.1107/S174430911202297X ]

Purification, crystallization and preliminary X-ray diffraction analysis of inner membrane complex (IMC) subcompartment protein 1 (ISP1) from Toxoplasma gondii

M. L. Tonkin, S. Brown, J. R. Beck, P. J. Bradley and M. J. Boulanger

Synopsis: To characterize the ISP family of proteins present in apicomplexan parasites, ISP1 from T. gondii was expressed, purified and crystallized. Two crystal forms (cubic and orthorhombic) were analyzed by X-ray diffraction and data were processed to 2.05 and 2.1 Å resolution, respectively.

Online 28 June 2012

 

Acta Cryst. (2012). F68, 835-838  [ doi:10.1107/S1744309112023597 ]

Two crystal forms of a helix-rich fatty acid- and retinol-binding protein, Na-FAR-1, from the parasitic nematode Necator americanus

M. Gabrielsen, M. F. Rey-Burusco, K. Griffiths, A. J. Roe, A. Cooper, B. O. Smith, M. W. Kennedy and B. Corsico

Synopsis: Na-FAR-1, a fatty acid- and retinol-binding protein, was expressed in bacteria, purified and crystallized. Crystals grew in two different morphologies under the same conditions.

Online 28 June 2012

 

Acta Cryst. (2012). F68, 839-841  [ doi:10.1107/S1744309112023603 ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of Deg5 from Arabidopsis thaliana

H. Fan, W. Sun, Z. Sun, F. Gao and W. Gong

Synopsis: A protease involved in turnover of a photosynthesis reaction centre protein has been crystallized and preliminary diffraction analysis was performed.

Online 28 June 2012

 

Acta Cryst. (2012). F68, 842-845  [ doi:10.1107/S1744309112022075 ]

Combining in situ proteolysis and mass spectrometry to crystallize Escherichia coli PgaB

D. J. Little, J. C. Whitney, H. Robinson, P. Yip, M. Nitz and P. L. Howell

Synopsis: Construct engineering and crystallization of E. coli PgaB using in situ proteolysis and mass spectrometry is reported.

Online 28 June 2012