Structural Biology and Crystallization Communications

 

Acta Cryst. (2012). F68, 999-1002  [ doi:10.1107/S1744309112030552 ]

Macrophage migration inhibitory factor covalently complexed with phenethyl isothiocyanate

J. D. A. Tyndall, H. Lue, M. T. Rutledge, J. Bernhagen, M. B. Hampton and S. M. Wilbanks

Synopsis: Macrophage migration inhibitory factor undergoes a localized conformational shift in response to covalent modification by phenethyl isothiocyanate, a natural compound with anti-inflammatory and anticancer properties. The inhibitor sits within a deep hydrophobic pocket and defines a potential target for the development of improved inhibitors.

PDB reference: 4f2k

Online 29 August 2012

 

Acta Cryst. (2012). F68, 1003-1007  [ doi:10.1107/S1744309112030667 ]

Structure of a UDP-glucose dehydrogenase from the hyperthermophilic archaeon Pyrobaculum islandicum

H. Sakuraba, T. Kawai, K. Yoneda and T. Ohshima

Synopsis: The crystal structure of an extremely thermostable UDP-glucose dehydrogenase from a hyperthermophile was determined.

PDB reference: 3vtf

Online 29 August 2012

 

Acta Cryst. (2012). F68, 1008-1012  [ doi:10.1107/S1744309112031004 ]

Structure of the starch-debranching enzyme barley limit dextrinase reveals homology of the N-terminal domain to CBM21

M. S. Møller, M. Abou Hachem, B. Svensson and A. Henriksen

Synopsis: The structure of barley limit dextrinase including all flexible loops has been traced at 1.9 Å resolution.

PDB reference: 4aio

Online 29 August 2012

 

Acta Cryst. (2012). F68, 1013-1017  [ doi:10.1107/S1744309112031570 ]

The role of active-site Phe87 in modulating the organic co-solvent tolerance of cytochrome P450 BM3 monooxygenase

J. Kuper, K. L. Tee, M. Wilmanns, D. Roccatano, U. Schwaneberg and T. S. Wong

Synopsis: Active-site Phe87 of cytochrome P450 BM3 protects the haem from DMSO molecule, thereby conferring higher organic co-solvent tolerance.

PDB references: 2x7y and 2x80

Online 29 August 2012

 

Acta Cryst. (2012). F68, 1018-1024  [ doi:10.1107/S1744309112031259 ]

Structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni at 2.7 Å resolution

I. G. Shabalin, P. J. Porebski, D. R. Cooper, M. Grabowski, O. Onopriyenko, S. Grimshaw, A. Savchenko, M. Chruszcz and W. Minor

Synopsis: The crystal structure of C. jejuni anabolic ornithine transcarbamoylase has been determined at a resolution of 2.7 Å in an unliganded state.

PDB reference: 3tpf

Online 29 August 2012

 

Acta Cryst. (2012). F68, 1025-1029  [ doi:10.1107/S1744309112033064 ]

Structural insights into ChpT, an essential dimeric histidine phosphotransferase regulating the cell cycle in Caulobacter crescentus

A. Fioravanti, B. Clantin, F. Dewitte, Z. Lens, A. Verger, E. G. Biondi and V. Villeret

Synopsis: The cell-cycle regulator ChpT of C. crescentus is a dimeric histidine phosphotransferase that resembles the typical structure of histidine kinases.

PDB reference: 4fpp

Online 29 August 2012

 

Acta Cryst. (2012). F68, 1030-1033  [ doi:10.1107/S1744309112031922 ]

Purification, crystallization and preliminary X-ray characterization of the Acetivibrio cellulolyticus type I cohesin ScaC in complex with the ScaB dockerin

K. Cameron, V. D. Alves, P. Bule, L. M. A. Ferreira, C. M. G. A. Fontes and S. Najmudin

Synopsis: A complex of a mutated ScaB dockerin with the third ScaC cohesin from A. cellulolyticus has been crystallized and data were collected from two different crystal forms to 1.5 and 6.0 Å resolution.

Online 30 August 2012

 

Acta Cryst. (2012). F68, 1034-1039  [ doi:10.1107/S1744309112032538 ]

Crystallization and preliminary crystal structure analysis of the ligand-binding domain of PqsR (MvfR), the Pseudomonas quinolone signal (PQS) responsive quorum-sensing transcription factor of Pseudomonas aeruginosa

N. Xu, S. Yu, S. Moniot, M. Weyand and W. Blankenfeldt

Synopsis: The ligand-binding domain of the transcription factor PqsR from P. aeruginosa has been crystallized and initial phases have been obtained using SAD data from seleno-L-methionine-labelled crystals.

Online 30 August 2012

 

Acta Cryst. (2012). F68, 1040-1047  [ doi:10.1107/S1744309112033052 ]

Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from Agrobacterium tumefaciens

S. C. Atkinson, C. Dogovski, R. C. J. Dobson and M. A. Perugini

Synopsis: Dihydrodipicolinate synthase from the plant pathogen A. tumefaciens has been cloned, expressed, purified and crystallized in its unliganded form, in the presence of its substrate pyruvate and in the presence of pyruvate and the allosteric inhibitor lysine. Diffraction data for the crystals were collected to a maximum resolution of 1.40 Å.

Online 30 August 2012

 

Acta Cryst. (2012). F68, 1048-1051  [ doi:10.1107/S1744309112031910 ]

Cloning, expression, crystallization and preliminary X-ray studies of the ferredoxin-NAD(P)⁺ reductase from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1

P. Liauw, T. Mashiba, M. Kopczak, K. Wiegand, N. Muraki, H. Kubota, Y. Kawano, M. Ikeuchi, T. Hase, M. Rögner and G. Kurisu

Synopsis: The cloning, expression and crystallization of a thermophilic ferredoxin-NAD(P)⁺ reductase were successfully carried out and resulting crystals were suitable for structural determination.

Online 30 August 2012

 

Acta Cryst. (2012). F68, 1052-1054  [ doi:10.1107/S1744309112032721 ]

Purification, crystallization and preliminary X-ray diffraction analysis of crotamine, a myotoxic polypeptide from the Brazilian snake Crotalus durissus terrificus

M. A. Coronado, D. Georgieva, F. Buck, A. H. Gabdoulkhakov, A. Ullah, P. J. Spencer, R. K. Arni and C. Betzel

Synopsis: Crotamine from C. durissus terrificus was crystallized and diffraction data were collected to a resolution of 1.9 Å.

Online 30 August 2012

 

Acta Cryst. (2012). F68, 1055-1059  [ doi:10.1107/S1744309112032526 ]

Crystallization and preliminary X-ray crystallographic analysis of subunit F (F_1-94), an essential coupling subunit of the eukaryotic V₁V_O-ATPase from Saccharomyces cerevisiae

S. Basak, A. M. Balakrishna, M. S. S. Manimekalai and G. Grüber

Synopsis: The S. cerevisiae V-ATPase subunit F (F_1-94) protein and the selenomethionyl form of its I69M mutant protein were produced, purified and crystallized. X-ray diffraction data were collected to resolutions of 2.64 and 2.3 Å, respectively, and the crystals belonged to the orthorhombic space group C222₁.

Online 30 August 2012

 

Acta Cryst. (2012). F68, 1060-1063  [ doi:10.1107/S1744309112031077 ]

Crystallographic study of the interaction of the anti-HIV lectin actinohivin with the (1-2)mannobiose moiety of gp120 HMTG

K. Suzuki, N. Ohbayashi, J. Jiang, X. Zhang, M. M. Hoque, M. Tsunoda, K. Murayama, H. Tanaka and A. Takénaka

Synopsis: The mature protein of the potent anti-HIV lectin actinohivin has been crystallized with high reproducibility in a new form without packing disorder.

Online 30 August 2012

 

Acta Cryst. (2012). F68, 1064-1066  [ doi:10.1107/S1744309112031053 ]

Preliminary X-ray crystallographic analysis of -carbonic anhydrase from Thiomicrospira crunogena XCL-2

N. Díaz Torres, G. González, S. Biswas, K. M. Scott and R. McKenna

Synopsis: Single crystals of -carbonic anhydrase from T. crunogena XCL-2 have been grown and were shown to diffract X-rays to 2.6 Å resolution. The crystals belonged to space group C2, with unit-cell parameters a = 127.1, b = 102.2, c = 105.0 Å, = 127.3°

Online 30 August 2012

 

Acta Cryst. (2012). F68, 1067-1069  [ doi:10.1107/S1744309112031508 ]

Expression, purification, crystallization and preliminary X-ray analysis of a novel N-substituted branched-chain L-amino-acid dioxygenase from Burkholderia ambifaria AMMD

H.-M. Qin, T. Miyakawa, A. Nakamura, Y.-L. Xue, T. Kawashima, T. Kasahara, M. Hibi, J. Ogawa and M. Tanokura

Synopsis: Diffraction data were collected to a limiting resolution of 2.4 Å from a crystal of selenomethionyl-labelled SadA, an L-amino-acid dioxygenase.

Online 30 August 2012

 

Acta Cryst. (2012). F68, 1070-1072  [ doi:10.1107/S1744309112030886 ]

Crystallization and preliminary X-ray crystallographic analysis of YgjG from Escherichia coli

S.-J. Yeo, J.-H. Jeong, S.-N. Yu and Y.-G. Kim

Synopsis: YgjG is a putrescine:2-oxoglutarate aminotransferase that belongs to the class III aminotransferases. In this study, YgjG from E. coli was overexpressed, purified and crystallized using the hanging-drop vapour-diffusion method.

Online 30 August 2012

 

Acta Cryst. (2012). F68, 1073-1076  [ doi:10.1107/S1744309112030813 ]

Crystallization and preliminary crystallographic analysis of the NheA component of the Nhe toxin from Bacillus cereus

D. Phung, M. Ganash, S. E. Sedelnikova, T. Lindbäck, P. E. Granum and P. J. Artymiuk

Synopsis: The NheA component of the B. cereus Nhe toxin was overexpressed in E. coli, purified and crystallized. Diffraction data were collected and processed to 2.05 Å resolution.

Online 31 August 2012

 

Acta Cryst. (2012). F68, 1077-1080  [ doi:10.1107/S1744309112030874 ]

Crystallization and preliminary X-ray crystallographic studies of Rab6A'(Q72L): a GTP-locked form

Y.-C. Shin, T.-H. Jang, J. H. Yoon, J.-H. Jeon and H. H. Park

Synopsis: Rab6A'(Q72L), a constitutively active GTP-binding form of Rab6A, was purified and crystallized. The crystals were found to belong to space group P22₁2₁, with unit-cell parameters a = 36.84, b = 96.78, c = 109.99 Å. The crystals were obtained at 293 K and diffracted to a resolution of 1.9 Å.

Online 31 August 2012

 

Acta Cryst. (2012). F68, 1081-1084  [ doi:10.1107/S174430911203103X ]

Crystallographic characterization of mouse AIM2 HIN-200 domain bound to a 15 bp and an 18 bp double-stranded DNA

M. W. Sung, T. Watts and P. Li

Synopsis: AIM2 is an innate immune sensor of microbial double-stranded DNA. The HIN-200 domain of mouse AIM2 bound to a 15 bp and an 18 bp dsDNA were crystallized and diffract to about 4.0 Å.

Online 31 August 2012

 

Acta Cryst. (2012). F68, 1085-1088  [ doi:10.1107/S1744309112030631 ]

Purification, crystallization and preliminary X-ray analysis of SGR6054, a Streptomyces homologue of the mycobacterial integration host factor mIHF

R. Nomoto, T. Tezuka, K. Miyazono, M. Tanokura, S. Horinouchi and Y. Ohnishi

Synopsis: A Streptomyces homologue of the mycobacterial integration host factor mIHF was heterologously produced, purified and crystallized in the presence of a 16-mer duplex DNA by the sitting-drop vapour-diffusion method. The best crystal diffracted X-rays to 2.22 Å resolution and belonged to space group C2.

Online 31 August 2012

 

Acta Cryst. (2012). F68, 1089-1093  [ doi:10.1107/S174430911203062X ]

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of succinyl-diaminopimelate desuccinylase (Rv1202, DapE) from Mycobacterium tuberculosis

L. Reinhard, J. Mueller-Dieckmann and M. S. Weiss

Synopsis: M. tuberculosis succinyl-diaminopimelate desuccinylase, the enzyme which catalyzes the seventh step of the lysine-biosynthesis pathway, has been cloned, expressed, purified and crystallized. Preliminary X-ray diffraction analysis indicated the presence of pseudo-merohedral twinning in space group P2₁, resulting in possible emulation of space group C222₁.

Online 31 August 2012

 

Acta Cryst. (2012). F68, 1094-1097  [ doi:10.1107/S1744309112030151 ]

Crystallization and preliminary X-ray analysis of Chandipura virus glycoprotein G

E. Baquero, L. Buonocore, J. K. Rose, S. Bressanelli, Y. Gaudin and A. A. Albertini

Synopsis: Chandipura virus glycoprotein ectodomain (Gth) was purified and crystallized at pH 7.5. X-ray diffraction data set was collected to a resolution of 3.1 Å.

Online 31 August 2012

 

Acta Cryst. (2012). F68, 1098-1101  [ doi:10.1107/S1744309112030527 ]

Crystallization and preliminary X-ray crystallographic analysis of the putative NADP(H)-dependent oxidoreductase YncB from Vibrio vulnificus

M.-K. Kim, Y. J. An, C.-S. Jeong and S.-S. Cha

Synopsis: In order to investigate its structure and function, the medium-chain dehydrogenase/reductase superfamily YncB-like protein from V. vulnificus was expressed, purified and crystallized. X-ray diffraction analyses of YncB and the YncB-NADP(H) complex are reported at resolutions of 1.85 and 1.81 Å, respectively.

Online 31 August 2012

 

Acta Cryst. (2012). F68, 1102-1105  [ doi:10.1107/S1744309112030278 ]

Purification, crystallization and preliminary X-ray analysis of uracil-DNA glycosylase from Sulfolobus tokodaii strain 7

A. Kawai, S. Higuchi, M. Tsunoda, K. T. Nakamura and S. Miyamoto

Synopsis: Uracil-DNA glycosylase from S. tokodaii strain 7 was overexpressed and purified. Crystals of the apo form and of a complex with uracil were obtained.

Online 31 August 2012

 

Acta Cryst. (2012). F68, 1106-1108  [ doi:10.1107/S174430911203271X ]

Crystallization and preliminary crystallographic studies of FoxE from Rhodobacter ferrooxidans SW2, an Fe^II oxidoreductase involved in photoferrotrophy

L. Pereira, I. H. Saraiva, R. Coelho, D. K. Newman, R. O. Louro and C. Frazão

Synopsis: Crystals of the R. ferrooxidans SW2 iron oxidoreductase FoxE were obtained and the phase problem was solved by Fe SAD at 2.44 Å resolution.

Online 31 August 2012

 

Acta Cryst. (2012). F68, 1109-1112  [ doi:10.1107/S1744309112033155 ]

Crystallization and preliminary X-ray characterization of the Vitis vinifera fucokinase:GDP-fucose pyrophosphorylase

S. Quirk

Synopsis: The Vitis vinifera dual-activity fucose and nucleotide-sugar metabolizing enzyme L-fucokinase:GDP-fucose pyrophosphorylase (FKP) has been purified to homogeneity and the 118.8 kDa monomeric protein has been crystallized by vapor diffusion in Zeppezauer tubes at 277 K.

Online 31 August 2012

 

Acta Cryst. (2012). F68, 1113-1115  [ doi:10.1107/S1744309112032629 ]

Production and crystallization of -phosphoglucomutase from Lactococcus lactis

P. Nogly, R. Castro, M. de Rosa, A. R. Neves, H. Santos and M. Archer

Synopsis: -Phosphoglucomutase from L. lactis, a homologue of human phosphomannomutase 1, was produced and crystallized. X-ray diffraction data were collected to 1.5 Å resolution.

Online 31 August 2012

 

Acta Cryst. (2012). F68, 1116-1119  [ doi:10.1107/S1744309112033088 ]

Crystallization and preliminary X-ray characterization of a type III cohesin-dockerin complex from the cellulosome system of Ruminococcus flavefaciens

O. Salama-Alber, Y. Gat, R. Lamed, L. J. W. Shimon, E. A. Bayer and F. Frolow

Synopsis: The cloning, expression, purification, crystallization and preliminary X-ray characterization of a trimodular type III cohesin-dockerin complex from the R. flavefaciens cellulosome system are described.

Online 31 August 2012

 

Acta Cryst. (2012). F68, 1120-1123  [ doi:10.1107/S1744309112034331 ]

Purification and crystallization of mono-ubiquitylated ubiquitin receptor Rpn10

T. Keren-Kaplan and G. Prag

Synopsis: A novel reconstitution system for the modification and purification of ubiquitylated proteins yielded the first diffracting crystals of a ubiquitylated substrate, namely Rpn10.

Online 31 August 2012

 

Acta Cryst. (2012). F68, 1124-1127  [ doi:10.1107/S1744309112033702 ]

Crystallization and preliminary diffraction studies of SFC-1, a carbapenemase conferring antibiotic resistance

M.-K. Hong, J. J. Lee, X. Wu, J.-K. Kim, B. C. Jeong, T.-V. Pham, S.-H. Kim, S. H. Lee and L.-W. Kang

Synopsis: The SFC-1 gene from S. fonticola was cloned and SFC-1 was expressed, purified and crystallized. X-ray diffraction data were collected from an SFC-1 crystal to 1.6 Å resolution.

Online 31 August 2012

 

Acta Cryst. (2012). F68, 1128-1133  [ doi:10.1107/S1744309112033519 ]

Crystallization and preliminary X-ray crystallographic analysis of Aquifex aeolicus SelA, a bacterial selenocysteine synthase

Y. Itoh, S. Sekine and S. Yokoyama

Synopsis: The bacterial selenocysteine synthase SelA from Aquifex aeolicus was crystallized and the diffraction resolution was improved by lysine-residue methylation, truncation of N-terminal region (N), and Lys-to-Ala point mutations. Phases were determined by using a selenomethionine-substituted crystal of the N mutant.

Online 31 August 2012

 

Acta Cryst. (2012). F68, 1134-1138  [ doi:10.1107/S1744309112033507 ]

Soaking of DNA into crystals of archaeal RNA polymerase achieved by desalting in droplets

M. N. Wojtas and N. G. A. Abrescia

Synopsis: RNA polymerase from S. shibatae in complex with DNA was crystallized using a nanoseeding method. Native RNAP crystals underwent an ad hoc procedure for DNA binding; one of these crystals diffracted to 4.3 Å resolution.

Online 31 August 2012