High-resolution structures of Thermus thermophilus enoyl-acyl carrier protein reductase in the apo form, in complex with NAD⁺ and in complex with NAD⁺ and triclosan

T. thermophilus enoyl-acyl carrier protein reductase was crystallized in the apo form, with NAD⁺ bound and with NAD⁺ and the inhibitor triclosan bound. The structures were solved by molecular replacement and refined at 1.50, 1.86 and 1.90 Å resolution, respectively. The structures are described, analysed and compared with those of enoyl-acyl carrier protein reductases from other species.

Cloning, expression, purification, crystallization and X-ray analysis of inositol monophosphatase from Mus musculus and Homo sapiens

M. musculus and H. sapiens inositol monophosphatase 1 were cloned, expressed, purified and crystallized. Diffraction data were collected and analysed at resolutions of 2.4 and 1.7 Å, respectively, and the structures were compared in order to identify any structural differences.

Structure of the [NiFe]-hydrogenase maturation protein HypF from Thermococcus kodakarensis KOD1

The crystal structure of full-length HypF from T. kodakarenesis has been determined.

Structure of the HECT C-lobe of the UBR5 E3 ubiquitin ligase

The structure of the HECT C-lobe of UBR5 shows a unique tertiary conformation. The sub-domain is also able to form a thioester bond with ubiquitin and may thus confer E2 binding specificity to UBR5.

Structure of β-1,4-mannanase from the common sea hare Aplysia kurodai at 1.05 Å resolution

The crystal structure of AkMan, a gastropoda β-1,4-mannanase prepared from the common sea hare Aplysia kurodai, was determined at 1.05 Å resolution. This is the first report on the three-dimensional structure of gastropoda β-1,4-mannanase.

Structure of d(CCGGGACCGG)₄ as a four-way junction at 1.6 Å resolution: new insights into solvent interactions

The crystal structure of a four-way DNA junction at 1.6 Å resolution provides new insights into solvent interactions and their implications.

Structures of a γ-aminobutyrate (GABA) transaminase from the s-triazine-degrading organism Arthrobacter aurescens TC1 in complex with PLP and with its external aldimine PLP–GABA adduct

The structure of the γ-aminobutyrate transaminase A1R958 from A. aurescens TC1 in complex with PLP–GABA reveals the active-site determinants of GABA binding in this enzyme.

Crystallization and preliminary X-ray diffraction analysis of tau protein microtubule-binding motifs in complex with Tau5 and DC25 antibody Fab fragments

Two 30-amino-acid peptides derived from the two microtubule-binding tau protein regions have been cocrystallized with monoclonal antibody Fab fragments and preliminary X-ray diffraction analysis has been performed.

Crystallization and preliminary X-ray diffraction analysis of two peptides from Alzheimer PHF in complex with the MN423 antibody Fab fragment

Crystals of the paired helical filaments (PHF) specific monoclonal antibody MN423 grown in the presence of two synthetic peptides derived from the PHF tau protein were analysed by X-ray techniques and the structure was solved by molecular replacement.

Crystallization and preliminary crystallographic studies of CbsA, a secretory exoglucanase from Xanthomonas oryzae pv. oryzae

The exoglucanase CbsA from X. oryzae has been overexpressed, purified and crystallized. The crystal diffracted to a resolution of 1.86 Å.

Expression, purification, crystallization and preliminary X-ray analysis of ribitol-5-phosphate cytidylyltransferase from Bacillus subtilis

The B. subtilis ribitol-5-phosphate cytidylyltransferase enzyme TarI was crystallized; determination of its structure will lead to structural and functional insight into the biosynthesis of wall teichoic acids.

Purification, crystallization and preliminary crystallographic analysis of the CBS-domain pair of cyclin M2 (CNNM2)

This work describes the purification and preliminary crystallographic analysis of the CBS-domain pair of the murine CNNM2 magnesium transporter, which consists of a pair of cystathionine β-synthase (CBS) motifs and has 100% sequence identity to its human homologue.

Cloning, purification, crystallization and preliminary X-ray analysis of two low-molecular-weight protein tyrosine phosphatases from Vibrio cholerae

Two protein tyrosine phosphatases, namely VcLMWPTP-1 and VcLMWPTP-2, from V. cholerae have been cloned, expressed, purified and crystallized.

Production and crystallization of the C-propeptide trimer from human procollagen III

The first crystallization of a fibrillar procollagen C-propeptide domain, that from human procollagen III, is described. Present throughout the Metaozoa in the form of ∼90 kDa trimers, this domain plays crucial roles in tissue homeostasis and disease.

Crystallization and preliminary X-ray studies of MutT1 (MSMEG_2390) from Mycobacterium smegmatis

MutT1 (MSMEG_2390) from M. smegmatis has been crystallized and preliminary X-ray studies of the crystals have been carried out.

Purification, crystallization and X-ray diffraction analysis of human dynamin-related protein 1 GTPase-GED fusion protein

The crystallization and initial diffraction analysis of human Drp1 GTPase-GED fusion protein are reported.

Purification, crystallization and preliminary X-ray diffraction analysis of a class P-III metalloproteinase (BmMP-III) from the venom of Bothrops moojeni

The P-III metalloproteinase from B. moojeni was crystallized and diffraction data were collected to a maximum resolution of 3.3 Å.

Crystallization and preliminary diffraction studies of GIM-1, a class B carbapenem-hydrolyzing β-lactamase

GIM-1 is a member of the class B carbapenemases and has a wide spectrum of activity against carbapenems, penicillins and extended-spectrum cephalosporins, but not aztreonam. In this study, GIM-1 was cloned, overexpressed and crystallized.

Improved crystallization of Escherichia coli ATP synthase catalytic complex (F₁) by introducing a phosphomimetic mutation in subunit ∊

A phosphomimetic mutation in subunit ∊ dramatically increases reproducibility for crystallization of Escherichia coli ATP synthase catalytic complex (F₁) (subunit composition α₃β₃γ∊). Diffraction data were collected to ∼3.15 Å resolution using synchrotron radiation.

Expression, purification, crystallization and preliminary X-ray analysis of the RecQ helicase catalytic core from Deinococcus radiodurans

The DrRecQ helicase catalytic core has been crystallized to a resolution of 2.9 Å. The determination of its structure will lead to structural and functional insight into the DNA repair mechanism.

Expression, purification, crystallization and X-ray analysis of 3-quinuclidinone reductase from Agrobacterium tumefaciens

The purification and crystallization of 3-quinuclidinone reductase from A. tumefaciens allowed the collection of a diffraction data set to 1.72 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of alanine racemase from Pseudomonas putida YZ-26

A recombinant alanine racemase from the Pseudomonas putida YZ-26, has been crystallized by the sitting-drop vapor-diffusion method and X-ray diffraction data were collected to 2.4 Å.

Crystallization and preliminary X-ray diffraction analysis of PsaA, the adhesive pilin subunit that forms the pH 6 antigen on the surface of Yersinia pestis

The pH 6 antigen Psa displayed on the surface of Yersinia pestis, the bacterium that causes plague in humans, consists of polymers of a single protein subunit termed PsaA. Donor-strand complemented PsaA was purified and crystallized.

Crystallization and preliminary X-ray diffraction studies of Xanthomonas campestris PNPase in the presence of c-di-GMP

Crystals of S1-domain-truncated X. campestris PNPase were grown in the presence of c-di-GMP and diffracted to a resolution of 2.00 Å.

Purification, crystallization and preliminary crystallographic analysis of Kar9p from Saccharomyces cerevisiae

Kar9p from S. cerevisiae was cloned, overexpressed, purified and crystallized, and the crystals were characterized.

Expression, purification and crystallization of the outer membrane lipoprotein GumB from Xanthomonas campestris

The outer membrane lipoprotein GumB from X. campestris was purified and crystallized by the hanging-drop vapour-diffusion method. A native data set was collected to a resolution of 2.54 Å.

Use of a repetitive seeding protocol to obtain diffraction-quality crystals of a putative human D-xylulokinase

A putative human D-xylulokinase enzyme has been expressed, purified and crystallized using a repetitive seeding protocol. The trigonal crystals belonged to space group P3₁ or P3₂, with unit-cell parameters a = b = 101.87, c = 158.85 Å, and diffracted X-rays to at least 2.7 Å resolution.

Purification, crystallization and preliminary X-ray diffraction analysis of the RNA-dependent RNA polymerase from Thosea asigna virus

The RNA-dependent RNA polymerase of Thosea asigna virus has been purified and crystallized in two different crystal forms. Preliminary characterization of P2₁2₁2 and C222₁ crystals is reported. Co-crystallization experiments in the presence of lutetium produced a heavy-atom derivative suitable for structure determination.

Recombinant portal protein from Staphylococcus epidermidis bacteriophage CNPH82 is a 13-subunit oligomer

Crystals of the portal protein from Staphylococcus epidermidis bacteriophage CNPH82, diffracting to ∼4.2 Å resolution, have been obtained. The protein is a 13-subunit oligomer both in solution and in the crystal.