issue contents

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X

November 2012 issue

Highlighted illustration

Cover illustration: Structure of the cold-shock-like protein from Rickettsia rickettsii (Gerarden et al., p. 1284).

structural communications


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Despite its high affinity for NADPH, AKR1a4 crystallized in the apo form, which is very rare for aldo-keto reductase enzymes.

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The structure of recombinant prolidase from the hyperthermophilic archaeon Thermococcus sibiricus (Tsprol) was determined at 2.6 Å resolution.

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The first structure of a glycerol-3-phosphate dehydrogenase from S. cerevisiae, GPD1, is reported at 2.45 Å resolution.


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The recombinant BPTI/Kunitz-type inhibitor rShPI-1A from the Caribbean sea anemone S. helianthus has been crystallized and its structure has been refined to 2.5 Å resolution and compared with known X-ray structures of BPTI-like Kunitz-type inhibitors.


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Binding of cisplatin to His15 in hen egg-white lysozyme in aqueous media is observed after prolonged chemical exposure for 15 months, in contrast to the lack of binding that was observed after 4 d in a previous study. Binding of carboplatin is seen in greater detail in the case of room-temperature data collection compared with cryo data collection.

crystallization communications


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The crystallization of both native P. putida transcriptional regulator PtxS and its complex with its DNA recognition sequence using the counter-diffusion method are reported.

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The FliH–FliI complex from the bacterial flagellar type III export apparatus has been expressed, purified and crystallized, and the crystals have been characterized by X-ray diffraction.

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The crystallization and preliminary crystallographic studies of the carboxy-terminal domain of D. melanogaster eukaryotic translation initiation factor 5C domain-containing protein are reported.

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This article describes the crystallization and preliminary crystallographic analysis of a protein construct (hCBS516–525) that contains the full-length cystathionine β-synthase from Homo sapiens (hCBS) and just lacks amino-acid residues 516–525.

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This manuscript describes the crystallization of the full-length enzyme cystathionine β-synthase (CBS) from Apis mellifera, which maintains 46% sequence identity with the human homolog. Mutations in CBS cause hereditary homocystinuria in humans.

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A vasoconstrictor PLA2 was purified from Agkistrodon halys pallas venom and the preliminary X-ray diffraction analysis had been described.

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The ETS domain of human Ergp55 was purified and crystallized in native, complexes with E74, and cfos promoter DNA sequences. The X-ray intensity data set was collected on ETS–cfos promoter DNA complex crystal at 3.1 Å resolution to analyze the structure by molecular replacement technique.

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The crystallization of 2-aminophenol 1,6-dioxygenase in complexes with its substrate and with an inhibitor is reported.

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Recombinant dihydroorotase domain of human CAD was expressed in E. coli, purified and crystallized. An X-ray diffraction data set was collected to 1.75 Å resolution.

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D. melanogaster Zucchini was expressed, purified and crystallized. X-ray diffraction data were collected to 1.75 Å resolution.


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ID2 is an inhibitor of DNA binding that is known to be highly unstable. This study describes the cloning, expression and purification of ID2 for crystallization, culminating in the collection of diffraction data.

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The myelin protein P2 is a peripheral membrane protein functional in lipid bilayer binding and stacking. In order to study the fine details of P2 structure and function, 14 point mutants of human P2 were generated and crystallized; a total of eight different crystal forms were obtained, some of which diffracted to atomic resolution.

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Native and Hg-derivative diffraction data from human α-L-iduronidase crystals were collected to 2.3 and 3.1 Å resolution, respectively. SIRAS phasing gave a high-quality electron-density map.

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Proliferating cell nuclear antigen from Litopenaeus vannamei was recombinantly expressed, purified and crystallized. Diffraction data were obtained and processed to 3 Å.

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Purification, crystallization, preliminary X-ray diffraction and molecular-replacement studies have been carried out on Clarias magur haemoglobin.

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An enantioselective esterase (pfEstA) from P. fluorescens KCTC 1767 was crystallized in space group P212121 and diffraction data were collected to a resolution of 1.9 Å.

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Crystallization and diffraction analysis of a Ca2+-dependent PI-PLC from Streptomyces is reported. Optimization of crystals was completed using a drop-pinning technique and heavy-atom soaks to achieve high-quality diffraction to 1.23 Å.

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High-quality crystals of uridine phosphorylase from Shewanella oneidensis were grown under microgravity conditions. X-ray diffraction data were collected to a resolution of 0.95 Å.

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The editing domain of threonyl-tRNA synthetase from the archaeon Aeropyrum pernix has been overexpressed, purified and crystallized. The crystal diffracted to a resolution of 1.66 Å.

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The expression and purification of uridine phosphorylase from V. cholerae and the crystallization and preliminary X-ray structure analysis of the complex of this enzyme with thymidine are reported.

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A complex comprising the LIM domains of the LIM-homeodomain protein Isl1 tethered to a peptide region of Ldb1 has been engineered, purified and crystallized. Crystals of this intramolecular complex diffracted to 3.10 Å resolution.

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The successful purification, crystallization and first data collection to 1.8 Å resolution of the putative transfer protein TraN from the Gram-positive conjugative plasmid pIP501 are reported.

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An ABC transporter from G. kaustophilus has been crystallized in space group I222. X-ray diffraction data have been collected to 1.60 Å resolution.

laboratory communications


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Ellman's reagent oxidized the free sulfhydryl group of cysteine in Anabaena CcbP protein, facilitating its crystallization.

obituaries


addenda and errata




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