Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 68, Part 11 (November 2012)



[Issue Author Index][Volume Author Index]
[Cover illustration] Cover illustration: Structure of the cold-shock-like protein from Rickettsia rickettsii (Gerarden et al., p. 1284).

structural communications


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Acta Cryst. (2012). F68, 1271-1274  [ doi:10.1107/S1744309112037128 ]

High-resolution structure of AKR1a4 in the apo form and its interaction with ligands

F. Faucher and Z. Jia

Synopsis: Despite its high affinity for NADPH, AKR1a4 crystallized in the apo form, which is very rare for aldo-keto reductase enzymes.

PDB reference: 4gac

Online 26 October 2012


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Acta Cryst. (2012). F68, 1275-1278  [ doi:10.1107/S174430911203761X ]

Influence of intermolecular contacts on the structure of recombinant prolidase from Thermococcus sibiricus

A. A. Trofimov, E. A. Slutskaya, K. M. Polyakov, P. V. Dorovatovskii, V. M. Gumerov and V. O. Popov

Synopsis: The structure of recombinant prolidase from the hyperthermophilic archaeon Thermococcus sibiricus (Tsprol) was determined at 2.6 Å resolution.

PDB reference: 4fkc

Online 26 October 2012


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Acta Cryst. (2012). F68, 1279-1283  [ doi:10.1107/S1744309112037736 ]

Structure of glycerol-3-phosphate dehydrogenase (GPD1) from Saccharomyces cerevisiae at 2.45 Å resolution

D. A. Alarcon, M. Nandi, X. Carpena, I. Fita and P. C. Loewen

Synopsis: The first structure of a glycerol-3-phosphate dehydrogenase from S. cerevisiae, GPD1, is reported at 2.45 Å resolution.

PDB reference: 4fgw

Online 26 October 2012


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Acta Cryst. (2012). F68, 1284-1288  [ doi:10.1107/S174430911203881X ]

Solution structure of the cold-shock-like protein from Rickettsia rickettsii

K. P. Gerarden, A. M. Fuchs, J. M. Koch, M. M. Mueller, D. R. Graupner, J. T. O'Rorke, C. D. Frost, H. A. Heinen, E. R. Lackner, S. J. Schoeller, P. G. House, F. C. Peterson and C. T. Veldkamp

Synopsis: The solution structure of the cold-shock-like protein from R. rickettsii, the causative agent of Rocky Mountain spotted fever, is reported.

PDB reference: 2lss

Online 26 October 2012


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Acta Cryst. (2012). F68, 1289-1293  [ doi:10.1107/S1744309112039085 ]

Structure of the recombinant BPTI/Kunitz-type inhibitor rShPI-1A from the marine invertebrate Stichodactyla helianthus

R. García-Fernández, T. Pons, A. Meyer, M. Perbandt, Y. González-González, D. Gil, M. de los Angeles Chávez, C. Betzel and L. Redecke

Synopsis: The recombinant BPTI/Kunitz-type inhibitor rShPI-1A from the Caribbean sea anemone S. helianthus has been crystallized and its structure has been refined to 2.5 Å resolution and compared with known X-ray structures of BPTI-like Kunitz-type inhibitors.

PDB reference: 3ofw

Online 26 October 2012


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Acta Cryst. (2012). F68, 1294-1299  [ doi:10.1107/S1744309112041796 ]

Structure of Escherichia coli RutC, a member of the YjgF family and putative aminoacrylate peracid reductase of the rut operon

A. A. Knapik, J. J. Petkowski, Z. Otwinowski, M. T. Cymborowski, D. R. Cooper, M. Chruszcz, W. M. Krajewska and W. Minor

Synopsis: The structure of the putative aminoacrylate peracid reductase RutC of the rut operon, a member of the YjgF family, is reported.

PDB reference: 3v4d

Online 26 October 2012


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Acta Cryst. (2012). F68, 1300-1306  [ doi:10.1107/S1744309112042005 ]

Room-temperature X-ray diffraction studies of cisplatin and carboplatin binding to His15 of HEWL after prolonged chemical exposure

S. W. M. Tanley, A. M. M. Schreurs, L. M. J. Kroon-Batenburg and J. R. Helliwell

Synopsis: Binding of cisplatin to His15 in hen egg-white lysozyme in aqueous media is observed after prolonged chemical exposure for 15 months, in contrast to the lack of binding that was observed after 4 d in a previous study. Binding of carboplatin is seen in greater detail in the case of room-temperature data collection compared with cryo data collection.

PDB references: 4g49, 4g4a, 4g4b, 4g4c and 4g4h

Online 26 October 2012


crystallization communications


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Acta Cryst. (2012). F68, 1307-1310  [ doi:10.1107/S1744309112028540 ]

In situ X-ray data collection from highly sensitive crystals of Pseudomonas putida PtxS in complex with DNA

E. Pineda-Molina, A. Daddaoua, T. Krell, J. L. Ramos, J. M. García-Ruiz and J. A. Gavira

Synopsis: The crystallization of both native P. putida transcriptional regulator PtxS and its complex with its DNA recognition sequence using the counter-diffusion method are reported.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1311-1314  [ doi:10.1107/S1744309112030801 ]

Crystallization and preliminary X-ray analysis of the FliH-FliI complex responsible for bacterial flagellar type III protein export

Y. Uchida, T. Minamino, K. Namba and K. Imada

Synopsis: The FliH-FliI complex from the bacterial flagellar type III export apparatus has been expressed, purified and crystallized, and the crystals have been characterized by X-ray diffraction.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1315-1317  [ doi:10.1107/S1744309112036512 ]

Crystallization and preliminary crystallographic studies of the W2 domain of Drosophila melanogaster eukaryotic translation initiation factor 5C domain-containing protein

H. Zhao, H. Wang, H. Liu, M. Teng and X. Li

Synopsis: The crystallization and preliminary crystallographic studies of the carboxy-terminal domain of D. melanogaster eukaryotic translation initiation factor 5C domain-containing protein are reported.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1318-1322  [ doi:10.1107/S1744309112037219 ]

Purification, crystallization and preliminary crystallographic analysis of human cystathionine [beta]-synthase

I. Oyenarte, T. Majtan, J. Ereño, M. A. Corral-Rodríguez, J. P. Kraus and L. A. Martínez-Cruz

Synopsis: This article describes the crystallization and preliminary crystallographic analysis of a protein construct (hCBS516-525) that contains the full-length cystathionine [beta]-synthase from Homo sapiens (hCBS) and just lacks amino-acid residues 516-525.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1323-1328  [ doi:10.1107/S1744309112038638 ]

Purification, crystallization and preliminary crystallographic analysis of the full-length cystathionine [beta]-synthase from Apis mellifera

I. Oyenarte, T. Majtan, J. Ereño, M. A. Corral-Rodríguez, J. Klaudiny, J. Majtan, J. P. Kraus and L. A. Martínez-Cruz

Synopsis: This manuscript describes the crystallization of the full-length enzyme cystathionine [beta]-synthase (CBS) from Apis mellifera, which maintains 46% sequence identity with the human homolog. Mutations in CBS cause hereditary homocystinuria in humans.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1329-1332  [ doi:10.1107/S1744309112038523 ]

Purification, crystallization and preliminary X-ray diffraction analysis of an acidic phospholipase A2 with vasoconstrictor activity from Agkistrodon halys pallas venom

Z. Zou, F. Zeng, L. Zhang, L. Niu, M. Teng and X. Li

Synopsis: A vasoconstrictor PLA2 was purified from Agkistrodon halys pallas venom and the preliminary X-ray diffraction analysis had been described.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1333-1336  [ doi:10.1107/S1744309112038675 ]

Purification, crystallization and preliminary X-ray crystallographic analysis of the ETS domain of human Ergp55 in complex with the cfos promoter DNA sequence

S. P. Gangwar, S. R. Meena and A. K. Saxena

Synopsis: The ETS domain of human Ergp55 was purified and crystallized in native, complexes with E74, and cfos promoter DNA sequences. The X-ray intensity data set was collected on ETS-cfos promoter DNA complex crystal at 3.1 Å resolution to analyze the structure by molecular replacement technique.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1337-1340  [ doi:10.1107/S1744309112038705 ]

Crystallization and preliminary crystallographic analysis of 2-aminophenol 1,6-dioxygenase complexed with substrate and with an inhibitor

D.-F. Li, J.-Y. Zhang, Y. Hou, L. Liu, S.-J. Liu and W. Liu

Synopsis: The crystallization of 2-aminophenol 1,6-dioxygenase in complexes with its substrate and with an inhibitor is reported.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1341-1345  [ doi:10.1107/S1744309112038857 ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the dihydroorotase domain of human CAD

N. Lallous, A. Grande-García, R. Molina and S. Ramón-Maiques

Synopsis: Recombinant dihydroorotase domain of human CAD was expressed in E. coli, purified and crystallized. An X-ray diffraction data set was collected to 1.75 Å resolution.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1346-1350  [ doi:10.1107/S1744309112038936 ]

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Zucchini from Drosophila melanogaster

S. Fukuhara, H. Nishimasu, L. Bonnefond, N. Matsumoto, R. Ishitani and O. Nureki

Synopsis: D. melanogaster Zucchini was expressed, purified and crystallized. X-ray diffraction data were collected to 1.75 Å resolution.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1351-1353  [ doi:10.1107/S1744309112038924 ]

Cloning, purification and preliminary X-ray crystallographic analysis of the OmpA-like domain of peptidoglycan-associated lipoprotein from Acinetobacter baumannii

J. H. Song, W. C. Lee, J. S. Park, S. I. Kim, J. C. Lee, C. Cheong and H.-Y. Kim

Synopsis: The crystallization of the OmpA-like domain of peptidoglycan-associated lipoprotein from A. baumannii is described.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1354-1358  [ doi:10.1107/S174430911203895X ]

Cloning, purification and preliminary X-ray data analysis of the human ID2 homodimer

M. V. Wong, P. Palasingam and P. R. Kolatkar

Synopsis: ID2 is an inhibitor of DNA binding that is known to be highly unstable. This study describes the cloning, expression and purification of ID2 for crystallization, culminating in the collection of diffraction data.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1359-1362  [ doi:10.1107/S1744309112039036 ]

Production and crystallization of a panel of structure-based mutants of the human myelin peripheral membrane protein P2

M. Lehtimäki, S. Laulumaa, S. Ruskamo and P. Kursula

Synopsis: The myelin protein P2 is a peripheral membrane protein functional in lipid bilayer binding and stacking. In order to study the fine details of P2 structure and function, 14 point mutants of human P2 were generated and crystallized; a total of eight different crystal forms were obtained, some of which diffracted to atomic resolution.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1363-1366  [ doi:10.1107/S1744309112040432 ]

Crystallization, X-ray diffraction analysis and SIRAS phasing of human [alpha]-L-iduronidase

N. Maita, H. Taniguchi and H. Sakuraba

Synopsis: Native and Hg-derivative diffraction data from human [alpha]-L-iduronidase crystals were collected to 2.3 and 3.1 Å resolution, respectively. SIRAS phasing gave a high-quality electron-density map.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1367-1370  [ doi:10.1107/S1744309112040444 ]

Crystallization and X-ray diffraction studies of crustacean proliferating cell nuclear antigen

J. S. Carrasco-Miranda, C. Cardona-Felix, A. A. Lopez-Zavala, E. de-la-Re-Vega, E. De la Mora, E. Rudiño-Piñera, R. R. Sotelo-Mundo and L. G. Brieba

Synopsis: Proliferating cell nuclear antigen from Litopenaeus vannamei was recombinantly expressed, purified and crystallized. Diffraction data were obtained and processed to 3 Å.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1371-1373  [ doi:10.1107/S1744309112040547 ]

Purification, crystallization, preliminary X-ray diffraction and molecular-replacement studies of catfish (Clarias magur) haemoglobin

S. M. Jaimohan, M. D. Naresh and A. B. Mandal

Synopsis: Purification, crystallization, preliminary X-ray diffraction and molecular-replacement studies have been carried out on Clarias magur haemoglobin.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1374-1377  [ doi:10.1107/S1744309112040626 ]

Characterization, crystallization and preliminary X-ray diffraction analysis of an (S)-specific esterase (pfEstA) from Pseudomonas fluorescens KCTC 1767: enantioselectivity for potential industrial applications

S. Kim, T. Duc Ngo, K. K. Kim and T. D. Kim

Synopsis: An enantioselective esterase (pfEstA) from P. fluorescens KCTC 1767 was crystallized in space group P212121 and diffraction data were collected to a resolution of 1.9 Å.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1378-1386  [ doi:10.1107/S1744309112041371 ]

Crystallization, optimization and preliminary X-ray characterization of a metal-dependent PI-PLC from Streptomyces antibioticus

M. R. Jackson and T. L. Selby

Synopsis: Crystallization and diffraction analysis of a Ca2+-dependent PI-PLC from Streptomyces is reported. Optimization of crystals was completed using a drop-pinning technique and heavy-atom soaks to achieve high-quality diffraction to 1.23 Å.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1387-1389  [ doi:10.1107/S1744309112041784 ]

Crystallization of uridine phosphorylase from Shewanella oneidensis MR-1 in the laboratory and under microgravity and preliminary X-ray diffraction analysis

T. N. Safonova, N. N. Mordkovich, K. M. Polyakov, V. A. Manuvera, V. P. Veiko and V. O. Popov

Synopsis: High-quality crystals of uridine phosphorylase from Shewanella oneidensis were grown under microgravity conditions. X-ray diffraction data were collected to a resolution of 0.95 Å.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1390-1393  [ doi:10.1107/S1744309112042066 ]

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analyses of threonyl-tRNA synthetase editing domain from Aeropyrum pernix

S. Ahmad, A. S. K. Sravankumar, S. P. Kruparani and R. Sankaranarayanan

Synopsis: The editing domain of threonyl-tRNA synthetase from the archaeon Aeropyrum pernix has been overexpressed, purified and crystallized. The crystal diffracted to a resolution of 1.66 Å.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1394-1397  [ doi:10.1107/S1744309112041401 ]

Expression, purification, crystallization and preliminary X-ray structure analysis of Vibrio cholerae uridine phosphorylase in complex with thymidine

A. A. Lashkov, A. G. Gabdulkhakov, I. I. Prokofev, T. A. Seregina, S. E. Sotnichenko, A. V. Lyashenko, A. A. Shtil, A. S. Mironov, C. Betzel and A. M. Mikhailov

Synopsis: The expression and purification of uridine phosphorylase from V. cholerae and the crystallization and preliminary X-ray structure analysis of the complex of this enzyme with thymidine are reported.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1398-1401  [ doi:10.1107/S1744309112040031 ]

Crystallization and diffraction of an Isl1-Ldb1 complex

M. S. Gadd, D. A. Jacques, J. M. Guss and J. M. Matthews

Synopsis: A complex comprising the LIM domains of the LIM-homeodomain protein Isl1 tethered to a peptide region of Ldb1 has been engineered, purified and crystallized. Crystals of this intramolecular complex diffracted to 3.10 Å resolution.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1402-1405  [ doi:10.1107/S174430911204184X ]

Crystallization and first data collection of the putative transfer protein TraN from the Gram-positive conjugative plasmid pIP501

N. Goessweiner-Mohr, C. Fercher, M. Y. Abajy, E. Grohmann and W. Keller

Synopsis: The successful purification, crystallization and first data collection to 1.8 Å resolution of the putative transfer protein TraN from the Gram-positive conjugative plasmid pIP501 are reported.

Online 30 October 2012


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Acta Cryst. (2012). F68, 1406-1408  [ doi:10.1107/S1744309112037918 ]

Purification, crystallization and preliminary X-ray diffraction studies of the ATP-binding subunit of an ABC transporter from Geobacillus kaustophilus

M. Manjula, K. J. Pampa, S. Madan Kumar, N. Kunishima and N. K. Lokanath

Synopsis: An ABC transporter from G. kaustophilus has been crystallized in space group I222. X-ray diffraction data have been collected to 1.60 Å resolution.

Online 30 October 2012


laboratory communications


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Acta Cryst. (2012). F68, 1409-1414  [ doi:10.1107/S1744309112034938 ]

Ellman's reagent in promoting crystallization and structure determination of Anabaena CcbP

X.-X. Fan, Y.-F. Zhou, X. Liu, L.-F. Li and X.-D. Su

Synopsis: Ellman's reagent oxidized the free sulfhydryl group of cysteine in Anabaena CcbP protein, facilitating its crystallization.

PDB reference: 3urg

Online 30 October 2012


obituaries


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Acta Cryst. (2012). F68, 1415-1416  [ doi:10.1107/S1744309112044132 ]

Dame Louise Napier Johnson (1940-2012)

K. S. Wilson and D. I. Stuart

Synopsis: Obituary for Dame Louise Napier Johnson.

Online 31 October 2012


addenda and errata


 

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Acta Cryst. (2012). F68, 1417  [ doi:10.1107/S1744309112033246 ]

Crystallization and preliminary X-ray diffraction analysis of the high molecular weight ketoacyl reductase FabG4 complexed with NADH. Corrigendum

D. Dutta, S. Bhattacharyya and A. K. Das

Synopsis: A corrigendum to the article by Dutta et al. [(2012) Acta Cryst. F68, 786-789].

Online 31 October 2012


 

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Acta Cryst. (2012). F68, 1418  [ doi:10.1107/S1744309112036482 ]

Crystallization and preliminary X-ray crystallographic analysis of an ice-binding protein (FfIBP) from Flavobacterium frigoris PS1. Addendum

H. Do, J. H. Lee, S. G. Lee and H. J. Kim

Synopsis: An addendum to the article by Do et al. [(2012) Acta Cryst. F68, 806-809].

Online 31 October 2012


 

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Acta Cryst. (2012). F68, 1419  [ doi:10.1107/S1744309112039875 ]

Cloning, expression, crystallization and preliminary X-ray crystallographic analysis of aspartyl aminopeptidase from the apeB gene of Pseudomonas aeruginosa. Retraction

S. Natarajan and R. Mathews

Synopsis: The article by Natarajan & Mathews [(2012) (2012) Acta Cryst. F68, 207-210] is retracted.

Online 31 October 2012


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