Figure 1
Sequence alignment of conserved sequence motifs I-IV of GH70 glucansucrases, adapted from Leemhuis et al. (2012BB17) and prepared with ESPript (Gouet et al., 1999BB6). NU, nucleophile; A/B, general acid/base; TS, transition-state stabilizer. Residues interacting with subsites -1, +1 and +2 are indicated with black boxes. The three residues from motif IV following the transition-state stabilizer are shown in yellow; the residue immediately following the transition-state stabilizer (Asn1134 in GTFA-[Delta]N) is indicated by a red triangle. LrGTFA, Lactobacillus reuteri 121 GTFA; LrGTF180, L. reuteri 180 GTF180; LrGTFO, L. reuteri ATC 55730 GTFO; LmDSRS, Leuconostoc mesenteroides NRRL B-512F dextransucrase; LmDSRBCB4, L. mesenteroides B-1299 DSRB CB4 dextransucrase; LmASR, L. mesenteroides B-1355 alternansucrase; SmGTF-SI, Streptococcus mutans glucosyltransferase GTF-SI; SoGTFR, S. oralis ATC10557 glucosyltransferase R; LmDSREG2, L. mesenteroides NRRL B-1299 DSR-E (glucan-binding domain and catalytic domain CD2).  [article HTML]

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