Structure of the α-1,6/α-1,4-specific glucansucrase GTFA from Lactobacillus reuteri 121

Pijning, T.; Vujičić-Žagar, A.; Kralj, S.; Dijkhuizen, L.; Dijkstra, B.W.

doi:10.1107/S1744309112044168

Figure 6
Comparison of the active site of GTFA- [Delta]

N (blue, catalytic domain A; green, domain B) and GTF180- [Delta]

N. Relevant residues from GTFA- [Delta]

N are shown with blue or green C atoms and black labels and residues from GTF180- [Delta]

N are shown with white C atoms and grey labels. (a) Superposition of GTFA- [Delta]

N with the GTF180- [Delta]

N D1025N-sucrose complex (subsites -1 and +1; PDB entry 3hz3 ; Vujicic-Zagar et al., 2010

). The three catalytic residues and the tyrosine residue below subsite -1 are shown in stick representation. The sucrose bound to GTF180- [Delta]

N is shown with yellow C atoms. (b) Superposition of GTFA- [Delta]

N with the GTF180- [Delta]

N-maltose complex (subsites +1 and +2; PDB entry 3kll ; Vujicic-Zagar et al., 2010

). Maltose from the GTF180- [Delta]

N complex, with its reducing-end glucosyl unit (subsite +2) stacking with a conserved tryptophan residue, is shown with yellow C atoms. The O1 atom of the acceptor sugar residue, which possibly interacts with Asn1134 in GTFA- [Delta]

N, is indicated with an asterisk (*).