Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 69, Part 1 (January 2013)



[Issue Author Index][Volume Author Index]
[Cover illustration] Cover illustration: A selection of crystals from articles published in Acta Cryst. F in 2012.

editorial


 

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Acta Cryst. (2013). F69, 1  [ doi:10.1107/S1744309112051950 ]

Crystals on the cover 2013

H. Einspahr and M. S. Weiss

Synopsis: Editorial.

Online 31 December 2012


structural communications


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Acta Cryst. (2013). F69, 2-5  [ doi:10.1107/S1744309112047422 ]

Exploring the cross-reactivity of S25-2: complex with a 5,6-dehydro-Kdo disaccharide

C. L. Brooks, K. Wimmer, P. Kosma, S. Müller-Loennies, L. Brade, H. Brade and S. V. Evans

Synopsis: The structure of the near germline antibody S25-2 in complex with an unnatural Kdo disaccharide highlights the remarkable plasticity in antigen recognition of germline antibodies.

PDB reference: 4hgw

Online 25 December 2012


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Acta Cryst. (2013). F69, 6-9  [ doi:10.1107/S1744309112048890 ]

Structure determination of LpxD from the lipopolysaccharide-synthesis pathway of Acinetobacter baumannii

J. Badger, B. Chie-Leon, C. Logan, V. Sridhar, B. Sankaran, P. H. Zwart and V. Nienaber

Synopsis: Crystal structures of the protein LpxD from A. baumannii were solved in apo forms that are suitable for structure-based antibacterial drug discovery.

PDB references: 4e75 and 4e79

Online 25 December 2012


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Acta Cryst. (2013). F69, 10-14  [ doi:10.1107/S1744309112047549 ]

Structure of Pisum sativum Rubisco with bound ribulose 1,5-bisphosphate

P. C. Loewen, A. L. Didychuk, J. Switala, R. Perez-Luque, I. Fita and M. C. Loewen

Synopsis: The crystallization and structure determination to 2.2 Å resolution is reported for a ribulose 1,5-bisphosphate-bound non-activated form of garden pea ribulose-1,5-bisphosphate carboxylase/oxygenase.

PDB reference: 4hhh

Online 25 December 2012


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Acta Cryst. (2013). F69, 15-19  [ doi:10.1107/S1744309112047276 ]

Structure of alanine racemase from Oenococcus oeni with bound pyridoxal 5'-phosphate

K. Palani, S. K. Burley and S. Swaminathan

Synopsis: Alanine racemase from O. oeni exists as a dimer in the crystal structure. Both monomers contribute to the two active sites present, one for each monomer.

PDB reference: 3co8

Online 25 December 2012


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Acta Cryst. (2013). F69, 20-24  [ doi:10.1107/S1744309112047562 ]

Use of europium ions for SAD phasing of lysozyme at the Cu K[alpha] wavelength

B. Vijayakumar and D. Velmurugan

Synopsis: Europium(III) ions bound to the surface of hen egg-white lysozyme were found to exhibit good anomalous signal facilitating SAD phasing using laboratory-source data and automated model building. The europium ion-binding sites were observed up to the 15[sigma] level.

PDB reference: 4h1p

Online 25 December 2012


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Acta Cryst. (2013). F69, 25-34  [ doi:10.1107/S1744309112044739 ]

The AEROPATH project targeting Pseudomonas aeruginosa: crystallographic studies for assessment of potential targets in early-stage drug discovery

L. Moynie, R. Schnell, S. A. McMahon, T. Sandalova, W. A. Boulkerou, J. W. Schmidberger, M. Alphey, C. Cukier, F. Duthie, J. Kopec, H. Liu, A. Jacewicz, W. N. Hunter, J. H. Naismith and G. Schneider

Synopsis: A focused strategy has been directed towards the structural characterization of selected proteins from the bacterial pathogen P. aeruginosa. The objective is to exploit the resulting structural data, in combination with ligand-binding studies, and to assess the potential of these proteins for early-stage antimicrobial drug discovery.

PDB references: 4es6, 4etr, 4exb, 4exa, 4avr, 4avy, 4b79, 4avf, 2xub, 4b7c and 4b7x

Online 25 December 2012


crystallization communications


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Acta Cryst. (2013). F69, 35-38  [ doi:10.1107/S1744309112049184 ]

Expression, purification and crystallization of the ectodomain of the envelope glycoprotein E2 from Bovine viral diarrhoea virus

O. Iourin, K. Harlos, K. El Omari, W. Lu, J. Kadlec, M. Iqbal, C. Meier, A. Palmer, I. Jones, C. Thomas, J. Brownlie, J. M. Grimes and D. I. Stuart

Synopsis: The cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the ectodomain of BVDV E2 are described.

Online 15 December 2012


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Acta Cryst. (2013). F69, 39-41  [ doi:10.1107/S1744309112045484 ]

Overexpression, crystallization and preliminary X-ray crystallographic analysis of the variable lymphocyte receptor 2913 ectodomain fused with internalin B

J. Y. Lee, H. S. Kim, I. W. Baek, J. M. Back, M. R. Han, S.-Y. Kong, J. H. Kim, R. N. Kirchdoerfer, J.-O. Kim, M. D. Cooper, I. A. Wilson, H.-J. Kim and B. W. Han

Synopsis: The VLR2913 ectodomain fused with internalin B was crystallized and diffraction data were collected to a maximum resolution of 2.04 Å.

Online 20 December 2012


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Acta Cryst. (2013). F69, 42-44  [ doi:10.1107/S1744309112048506 ]

Purification, crystallization and preliminary X-ray crystallographic analysis of diaminopimelate epimerase from Acinetobacter baumannii

J. S. Park, W. C. Lee, J. H. Song, S. I. Kim, J. C. Lee, C. Cheong and H.-Y. Kim

Synopsis: The crystallization and preliminary X-ray crystallographic analysis of diaminopimelate epimerase from A. baumannii are reported.

Online 20 December 2012


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Acta Cryst. (2013). F69, 45-48  [ doi:10.1107/S1744309112048002 ]

Crystallization and preliminary X-ray analysis of human MTH1 with a homogeneous N-terminus

Y. Koga, M. Inazato, T. Nakamura, C. Hashikawa, M. Chirifu, A. Michi, T. Yamashita, S. Toma, A. Kuniyasu, S. Ikemizu, Y. Nakabeppu and Y. Yamagata

Synopsis: A mutant human MTH1 protein [hMTH1(G2K)] with a homogeneous N-terminus produced high-quality crystals which diffracted to near 1.1 Å resolution using synchrotron radiation.

Online 20 December 2012


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Acta Cryst. (2013). F69, 49-52  [ doi:10.1107/S1744309112047124 ]

Purification, crystallization and preliminary X-ray diffraction analysis of the Staphylococcus epidermidis extracellular serine protease Esp

K. Vengadesan, K. Macon, S. Sugumoto, Y. Mizunoe, T. Iwase and S. V. L. Narayana

Synopsis: The expression, purification, crystallization and preliminary crystallographic characterization of the extracellular serine protease Esp from S. epidermidis are reported.

Online 20 December 2012


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Acta Cryst. (2013). F69, 53-56  [ doi:10.1107/S1744309112048257 ]

Preliminary crystallographic analysis of a polyadenylate synthase from Megavirus

A. Lartigue, S. Jeudy, L. Bertaux and C. Abergel

Synopsis: The preliminary crystallographic analysis of Megavirus chilensis Mg561, which has a predicted polyadenylate synthase function, is reported. The crystals belonged to space group P212121, with two monomers per asymmetric unit.

Online 20 December 2012


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Acta Cryst. (2013). F69, 57-60  [ doi:10.1107/S1744309112049032 ]

Overexpression, crystallization and preliminary X-ray crystallographic analysis of the phosphotriesterase from Mycobacterium tuberculosis

L. Zhang, R. Chen, Z. Dong and X. Li

Synopsis: Crystals of the phosphotriesterase from M. tuberculosis were obtained and diffraction data were collected and processed to 2.27 Å resolution. An analytical ultracentrifugation experiment suggested that mPHP exists as dimers in solution.

Online 20 December 2012


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Acta Cryst. (2013). F69, 61-64  [ doi:10.1107/S174430911204804X ]

Crystallization and preliminary X-ray diffraction studies of Drosophila melanogaster G[alpha]o-subunit of heterotrimeric G protein in complex with the RGS domain of CG5036

S. Tishchenko, A. Gabdulkhakov, U. Tin, O. Kostareva, C. Lin and V. L. Katanaev

Synopsis: D. melanogaster G[alpha]o-subunit and the RGS domain of its interacting partner CG5036 have been overproduced and purified; the crystallization and preliminary X-ray crystallographic analysis of the complex of the two proteins are reported.

Online 20 December 2012


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Acta Cryst. (2013). F69, 65-68  [ doi:10.1107/S1744309112045095 ]

Crystallization and preliminary X-ray crystallographic analysis of UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase (VvGT5) from the grapevine Vitis vinifera

E. Mizohata, T. Okuda, S. Hatanaka, T. Nakayama, M. Horikawa, T. Nakayama, E. Ono and T. Inoue

Synopsis: The UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase (VvGT5) from the grapevine V. vinifera was purified and crystallized. The best crystal diffracted X-rays to 2.2 Å resolution and belonged to space group P6122.

Online 25 December 2012


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Acta Cryst. (2013). F69, 69-72  [ doi:10.1107/S1744309112048774 ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of Arabidopsis thaliana Deg8

X. Shan, W. Sun, H. Fan, M. Jia, F. Gao and W. Gong

Synopsis: Crystals of Deg8, an ATP-independent serine endopeptidase from A. thaliana, were monoclinic, belonging to space group C2 with unit-cell parameters a = 129.5, b = 124.2, c = 93.3 Å, [beta] = 132.4°, and diffracted to 2.0 Å resolution.

Online 25 December 2012


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Acta Cryst. (2013). F69, 73-76  [ doi:10.1107/S174430911205049X ]

Crystallization and preliminary X-ray diffraction analysis of the organophosphorus hydrolase OPHC2 from Pseudomonas pseudoalcaligenes

G. Gotthard, J. Hiblot, D. Gonzalez, E. Chabrière and M. Elias

Synopsis: An organophosphorus hydrolase from P. pseudoalcaligenes named OPHC2 has been crystallized. Combined with biochemical characterization, it is expected that the structure of this protein will provide insight into the catalytic mechanism of organophosphorus hydrolysis and will highlight the role of key residues involved in substrate specificity.

Online 25 December 2012


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Acta Cryst. (2013). F69, 77-79  [ doi:10.1107/S1744309112049330 ]

Crystallization and preliminary X-ray diffraction analysis of YisP protein from Bacillus subtilis subsp. subtilis strain 168

Y. Hu, S. Jia, F. Ren, C.-H. Huang, T.-P. Ko, D. A. Mitchell, R.-T. Guo and Y. Zheng

Synopsis: A bacteria biofilm formation involved enzyme, BsYisP, from Bacillus subtilis subsp. subtilis strain 168, was crystallized and diffracted to 1.92 Å.

Online 25 December 2012


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Acta Cryst. (2013). F69, 80-82  [ doi:10.1107/S1744309112049408 ]

Crystallization and preliminary X-ray diffraction analysis of the Nif3-family protein MJ0927 from Methanocaldococcus jannaschii

S.-M. Kuan, H.-C. Chen, C.-H. Huang, C.-H. Chang, S.-C. Chen, C. S. Yang and Y. Chen

Synopsis: The hypothetical protein MJ0927 of the Nif3 family has been crystallized and X-ray diffraction data have been collected to a resolution of 2.47 Å.

Online 25 December 2012


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