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Figure 1
Purification of recombinant AbDapF. The results of the purification steps were detected on an SDS–PAGE gel. (a) Lane M, molecular-weight markers (labelled in kDa); lane 1, crude cell lysate; lane 2, pellet; lane 3, supernatant; lanes 4–5, flowthrough; lanes 6–7, washing fractions; lanes 8–9, fractions eluted from a HisTrap FF column. (b) Lane M, molecular-weight markers (labelled in kDa); lanes 1–7, fractions eluted from a HiLoad 16/60 Superdex 75 size-exclusion column.

ISSN: 2053-230X
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