Purification, crystallization and preliminary X-ray crystallographic analysis of diaminopimelate epimerase from Acinetobacter baumannii

Park, J.S.; Lee, W.C.; Song, J.H.; Kim, S.I.; Lee, J.C.; Cheong, C.; Kim, H.-Y.

doi:10.1107/S1744309112048506

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 1
Purification of recombinant AbDapF. The results of the purification steps were detected on an SDS–PAGE gel. (a) Lane M, molecular-weight markers (labelled in kDa); lane 1, crude cell lysate; lane 2, pellet; lane 3, supernatant; lanes 4–5, flowthrough; lanes 6–7, washing fractions; lanes 8–9, fractions eluted from a HisTrap FF column. (b) Lane M, molecular-weight markers (labelled in kDa); lanes 1–7, fractions eluted from a HiLoad 16/60 Superdex 75 size-exclusion column.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 69| Part 1| December 2013| Pages 42-44

doi:10.1107/S1744309112048506

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