Structure of Pisum sativum Rubisco with bound ribulose 1,5-bisphosphate

Loewen, P.C.; Didychuk, A.L.; Switala, J.; Perez-Luque, R.; Fita, I.; Loewen, M.C.

doi:10.1107/S1744309112047549

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 1
Preparation of diffracting crystals of PsRubisco. (a) Purification of PsRubisco. SDS–PAGE showing ammonium sulfate (AS) fractions. The 40% fraction contained the highest proportion of Rubisco and was used directly for crystallization after dialysis to remove AS. L, large subunit; S, small subunit. (b) Crystals of PsRubisco. The approximate dimensions of the PsRubisco crystals were 50 × 50 × 20 µm.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 69| Part 1| December 2013| Pages 10-14

doi:10.1107/S1744309112047549

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