Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 69, Part 3 (March 2013)

structural communications

Acta Cryst. (2013). F69, 228-236    [ doi:10.1107/S1744309113000961 ]

Structure of Vibrio cholerae ribosome hibernation promoting factor

H. De Bari and E. A. Berry

Abstract: The X-ray crystal structure of ribosome hibernation promoting factor (HPF) from Vibrio cholerae is presented at 2.0 Å resolution. The crystal was phased by two-wavelength MAD using cocrystallized cobalt. The asymmetric unit contained two molecules of HPF linked by four Co atoms. The metal-binding sites observed in the crystal are probably not related to biological function. The structure of HPF has a typical [beta]-[alpha]-[beta]-[beta]-[beta]-[alpha] fold consistent with previous structures of YfiA and HPF from Escherichia coli. Comparison of the new structure with that of HPF from E. coli bound to the Thermus thermophilus ribosome [Polikanov et al. (2012), Science, 336, 915-918] shows that no significant structural changes are induced in HPF by binding.

PDB reference: 4hei

Keywords: Vibrio cholerae; ribosome hibernation; hibernation promotion factor; ribosome-binding proteins; stationary phase; cold shock; MAD; cobalt; metal-binding site.

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