Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 69, Part 5 (May 2013)



[Issue Author Index][Volume Author Index]
[Cover illustration] Cover illustration: Structure of the CARD8 caspase-recruitment domain (Jin et al., p. 482).

structural communications


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Acta Cryst. (2013). F69, 472-476  [ doi:10.1107/S1744309113007392 ]

Structure of the catalytic domain of the Tannerella forsythia matrix metallopeptidase karilysin in complex with a tetrapeptidic inhibitor

T. Guevara, M. Ksiazek, P. D. Skottrup, N. Cerdà-Costa, S. Trillo-Muyo, I. de Diego, E. Riise, J. Potempa and F. X. Gomis-Rüth

Synopsis: The high-resolution crystal structure of the complex between the catalytic domain of karilysin and the tetrapeptide SWFP has been determined.

PDB reference: 4in9

Online 27 April 2013


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Acta Cryst. (2013). F69, 477-481  [ doi:10.1107/S1744309113008579 ]

Structure of the Yersinia pestis tip protein LcrV refined to 1.65 Å resolution

S. Chaudhury, K. P. Battaile, S. Lovell, G. V. Plano and R. N. De Guzman

Synopsis: Here, the crystal structure of Yersinia pestis tip protein LcrV is reported at a resolution of 1.65 Å.

PDB reference: 4jbu

Online 27 April 2013


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Acta Cryst. (2013). F69, 482-487  [ doi:10.1107/S1744309113010075 ]

The structure of the CARD8 caspase-recruitment domain suggests its association with the FIIND domain and procaspases through adjacent surfaces

T. Jin, M. Huang, P. Smith, J. Jiang and T. S. Xiao

Synopsis: The structure of the CARD8 caspase-recruitment domain suggests that its regulatory functions may be mediated by electrostatic attractions between the CARD8 CARD and its partner domains.

PDB reference: 4ikm

Online 27 April 2013


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Acta Cryst. (2013). F69, 488-493  [ doi:10.1107/S1744309113010622 ]

Expression, purification, crystallization and X-ray crystallographic studies of different redox states of the active site of thioredoxin 1 from the whiteleg shrimp Litopenaeus vannamei

A. A. Campos-Acevedo, K. D. Garcia-Orozco, R. R. Sotelo-Mundo and E. Rudiño-Piñera

Synopsis: Analysis of the different redox states of the catalytic cysteines in four crystallographic structures of thioredoxin 1 from the Pacific whiteleg shrimp L. vannamei highlights their reactivity and corroborates the existence of a structural dimer mediated by an interface of 12 residues which includes a disulfide bridge between the Cys73 residues of each monomer.

PDB references: 4aj6, 4aj8, 3zzx and 4aj7

Online 27 April 2013


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Acta Cryst. (2013). F69, 494-498  [ doi:10.1107/S1744309113010762 ]

Cocrystal structure of the ICAP1 PTB domain in complex with a KRIT1 peptide

W. Liu and T. J. Boggon

Synopsis: The cocrystal structure of the PTB domain of integrin cytoplasmic domain-associated protein-1 (ICAP1) in complex with a 29-residue fragment of Krev/Rap1 interaction trapped-1 (KRIT1) has been determined.

PDB reference: 4jif

Online 27 April 2013


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Acta Cryst. (2013). F69, 499-502  [ doi:10.1107/S1744309113010841 ]

Triosephosphate isomerase is a common crystallization contaminant of soluble His-tagged proteins produced in Escherichia coli

G. Kozlov, R. Vinaik and K. Gehring

Synopsis: Crystals of E. coli triosephosphate isomerase were obtained as a contaminant and its structure was determined to 1.85 Å resolution.

PDB reference: 4iot

Online 30 April 2013


crystallization communications


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Acta Cryst. (2013). F69, 503-505  [ doi:10.1107/S1744309113005344 ]

Crystallization and preliminary X-ray analysis of a UDP-MurNAc-tripeptide D-alanyl-D-alanine-adding enzyme (PaMurF) from Pseudomonas aeruginosa

V. Majce, K. M. Ruane, S. Gobec and D. I. Roper

Synopsis: P. aeruginosa UDP-MurNAc-tripeptide:D-Ala-D-Ala ligase (MurF) was crystallized in the presence of substrate and diffraction data were collected to 1.92 Å resolution.

Online 30 April 2013


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Acta Cryst. (2013). F69, 506-509  [ doi:10.1107/S1744309113006398 ]

Expression, purification, crystallization and preliminary X-ray structure analysis of wild-type and L(M196)H-mutant Rhodobacter sphaeroides reaction centres

A. G. Gabdulkhakov, T. Y. Fufina, L. G. Vasilieva, U. Mueller and V. A. Shuvalov

Synopsis: The expression, purification, crystallization and preliminary crystallographic characterization of wild-type and L(M196)H-mutant reaction centres of Rba. sphaeroides strain RV are reported.

Online 30 April 2013


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Acta Cryst. (2013). F69, 510-514  [ doi:10.1107/S1744309113006945 ]

Purification, crystallization and preliminary X-ray analysis of the strawberry allergens Fra a 1E and Fra a 3 in the presence of catechin

A. Casañal, U. Zander, F. Dupeux, V. Valpuesta and J. A. Marquez

Synopsis: The crystallization and preliminary X-ray diffraction analysis of the strawberry PR-10 proteins Fra a 1E and Fra a 3 in the presence of the natural flavonoid (+)-catechin are presented.

Online 30 April 2013


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Acta Cryst. (2013). F69, 515-519  [ doi:10.1107/S1744309113007045 ]

Purification, crystallization and preliminary crystallographic analysis of 3-hydroxyacyl-CoA dehydrogenase from Caenorhabditis elegans

Y. Xu and F. Sun

Synopsis: The 3-hydroxyacyl-CoA dehydrogenase from Ceanorhabditis elegans was overexpressed, purified and crystallized in two different space groups (P1 and P212121). The molecular dimers found in one asymmetric unit from both space groups are identical but each packs in a distinct manner.

Online 30 April 2013


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Acta Cryst. (2013). F69, 520-523  [ doi:10.1107/S1744309113007161 ]

Preliminary X-ray crystallographic studies of an N-terminal domain of unknown function from a putative glycosyltransferase from Streptococcus parasanguinis

H. Zhang, F. Zhu, L. Ding, M. Zhou, R. Wu and H. Wu

Synopsis: A streptococcal domain of unknown function 1792 has been crystallized.

Online 30 April 2013


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Acta Cryst. (2013). F69, 524-527  [ doi:10.1107/S1744309113007148 ]

Expression, purification and preliminary crystallographic analysis of the T6SS effector protein Tse3 from Pseudomonas aeruginosa

D. Lu, G. Shang, Q. Yu, H. Zhang, Y. Zhao, H. Cang, L. Gu, S. Xu and Y. Huang

Synopsis: Tse3, one of the effectors of the type VI secretion system in Pseudomonas aeruginosa, has been crystallized and diffracted to 1.5 Å resolution.

Online 30 April 2013


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Acta Cryst. (2013). F69, 528-531  [ doi:10.1107/S1744309113007288 ]

Expression, crystallization and preliminary X-ray crystallographic analysis of aldehyde dehydrogenase (ALDH) from Bacillus cereus

H.-P.-T. Ngo, S.-H. Hong, D.-K. Oh and L.-W. Kang

Synopsis: Aldehyde dehydrogenase (ALDH) catalyses the oxidation of aldehydes using NAD(P)+ as a cofactor. The aldh gene from B. cereus was cloned; the protein was expressed, purified and crystallized, and a preliminary X-ray crystallography analysis was performed.

Online 30 April 2013


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Acta Cryst. (2013). F69, 532-534  [ doi:10.1107/S174430911300763X ]

Crystallization and preliminary X-ray diffraction analysis of the TetR-family transcriptional repressor YhgD from Bacillus halodurans

H. K. Yeo, Y. W. Park, J. Kang and J. Y. Lee

Synopsis: Triclinic crystals of YhgD from B. halodurans have been obtained. X-ray data have been collected to 1.9 Å resolution using synchrotron radiation.

Online 30 April 2013


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Acta Cryst. (2013). F69, 535-539  [ doi:10.1107/S1744309113007811 ]

Cloning, overexpression, purification and crystallization of the CRN12 coiled-coil domain from Leishmania donovani

V. K. Srivastava, A. K. Rana, A. A. Sahasrabuddhe, C. M. Gupta and J. V. Pratap

Synopsis: The L. donovani coronin CRN12 coiled-coil domain (5.8 kDa) was cloned, overexpressed and purified to homogeneity. Crystals of recombinant L. donovani coronin CRN12 coiled-coil domain were grown by vapour diffusion using a hanging-drop setup.

Online 30 April 2013


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Acta Cryst. (2013). F69, 540-543  [ doi:10.1107/S1744309113008051 ]

Crystallization and initial X-ray data of abscisic acid receptor PYL3 in the presence of (-)-ABA

X. Zhang, Q. Zhang and G. Wang

Synopsis: The complex of the abscisic acid receptor PYL3 with (-)-ABA was crystallized and refined to obtain high-quality diffraction data. Diffraction data were collected and processed at 2.65 Å resolution.

Online 30 April 2013


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Acta Cryst. (2013). F69, 544-546  [ doi:10.1107/S1744309113008063 ]

Purification, crystallization and preliminary X-ray crystallographic analysis of the lumenal domain of the ER-vesicle protein Erv41p from Saccharomyces cerevisiae

E. I. Biterova, M. Svärd, D. D. D. Possner and J. E. Guy

Synopsis: The lumenal domain of Erv41p from S. cerevisiae has successfully been expressed, purified and crystallized. X-ray diffraction data were collected to a resolution of 2.0 Å.

Online 30 April 2013


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Acta Cryst. (2013). F69, 547-550  [ doi:10.1107/S1744309113008075 ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of a core fragment of FlgG, a bacterial flagellar rod protein

Y. Saijo-Hamano, H. Matsunami, K. Namba and K. Imada

Synopsis: A core fragment of Salmonella FlgG has been expressed, purified and crystallized, and X-ray diffraction data sets were collected to 2.0 Å resolution.

Online 30 April 2013


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Acta Cryst. (2013). F69, 551-554  [ doi:10.1107/S1744309113008233 ]

Crystallization and preliminary crystallographic analysis of an Escherichia coli-selected mutant of the nuclease domain of the metallonuclease colicin E7

A. Czene, E. Tóth, B. Gyurcsik, H. Otten, J.-C.N. Poulsen, L. Lo Leggio, S. Larsen, H. E. M. Christensen and K. Nagata

Synopsis: An N-terminally truncated mutant of the colicin E7 nuclease domain was crystallized and diffraction data set was collected to 1.6 Å resolution.

Online 30 April 2013


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Acta Cryst. (2013). F69, 555-558  [ doi:10.1107/S1744309113008385 ]

SAD phasing towards structure determination of a thermostable Rieske ferredoxin with a novel stabilizing disulfide bridge

T. M. Bandeiras, M. C. Freitas, D. Petrasch, A. Kletzin and C. Frazão

Synopsis: Crystallization and in-house SAD phasing unveils the structure of a unique thermophilic Rieske ferredoxin with a stabilizing disulfide bridge.

Online 30 April 2013


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Acta Cryst. (2013). F69, 559-561  [ doi:10.1107/S1744309113009664 ]

Expression, purification and crystallization of the C-terminal LRR domain of Streptococcus pyogenes protein 0843

T. Haikarainen, V. Loimaranta, C. Prieto-Lopez, P. Battula, J. Finne and A. C. Papageorgiou

Synopsis: The C-terminal LRR domain of S. pyogenes protein 0843 was overexpressed in E. coli, purified and crystallized. A complete data set to 1.59 Å resolution was collected using synchrotron radiation.

Online 30 April 2013


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Acta Cryst. (2013). F69, 562-565  [ doi:10.1107/S1744309113009925 ]

Crystallization and preliminary X-ray analysis of the CRP-cAMP-DNA (full length) complex

J. Huang, J. Liu, W. Tao, Z. Yang, R. Qiu, S. Yu and C. Ji

Synopsis: The Escherichia coli cyclic AMP receptor protein (CRP) crystals were obtained and diffracted at a 2.9 Å resolution, which belonged to the space group P3121, with unit-cell parameters a = b = 76.03, c = 144.00 Å. The asymmetric unit was found to contain one protein molecule and a half 38 bp full-length double-stranded DNA fragment with a Matthews coefficient of 2.62 Å3 Da-1 and a solvent content of 53.14%.

Online 30 April 2013


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Acta Cryst. (2013). F69, 566-569  [ doi:10.1107/S1744309113010427 ]

Cloning, expression, purification and preliminary X-ray diffraction studies of a mycobacterial protein implicated in bacterial survival in macrophages

A. E. Shahine, P. Y. Chan, D. Littler, J. Vivian, R. Brammananth, P. K. Crellin, R. L. Coppel, J. Rossjohn and T. Beddoe

Synopsis: The cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of MSMEG_5817 is described.

Online 30 April 2013


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Acta Cryst. (2013). F69, 570-573  [ doi:10.1107/S1744309113010750 ]

Cloning, expression, purification, crystallization and preliminary X-ray analysis of EaLsc, a levansucrase from Erwinia amylovora

L. Caputi, M. Cianci and S. Benini

Synopsis: EaLsc, a levansucrase from E. amylovora, has been cloned, expressed, purified and crystallized. X-ray crystallographic analysis and data collection to 2.77 Å resolution have been carried out. The structure was solved by molecular replacement and refinement is in progress.

Online 30 April 2013


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Acta Cryst. (2013). F69, 574-577  [ doi:10.1107/S1744309113010749 ]

Purification, crystallization and preliminary X-ray diffraction analysis of Omp6, a protoilludene synthase from Omphalotus olearius

M. B. Quin, G. Wawrzyn and C. Schmidt-Dannert

Synopsis: The expression, purification and crystallization of Omp6, a protoilludene synthase from O. olearius, are described.

Online 30 April 2013


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Acta Cryst. (2013). F69, 578-580  [ doi:10.1107/S1744309113010683 ]

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of Rv2606c from Mycobacterium tuberculosis H37Rv

S. Kim and K.-J. Kim

Synopsis: The pyridoxal biosynthesis lyase (PdxS) from Mycobacterium tuberculosis was crystallized and diffracted to a resolution of 1.8 Å. The crystals were found to belong to space group I222 or I212121, with unit-cell parameters a = 110.75, b = 126.08, c = 180.82 Å, [alpha] = [beta] = [gamma] = 90°.

Online 30 April 2013


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Acta Cryst. (2013). F69, 581-584  [ doi:10.1107/S1744309113010725 ]

Purification, crystallization and preliminary X-ray analysis of the effector domain of AlsR, an LysR-type transcriptional regulator from Bacillus subtilis

C. Frädrich, J. Krausze, N. Quade, D. Heinz, D. Jahn and E. Härtig

Synopsis: The cloning, purification, crystallization and preliminary crystallographic analysis of the effector-binding domain of the transcriptional regulator AlsR from B. subtilis are described.

Online 30 April 2013


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Acta Cryst. (2013). F69, 585-587  [ doi:10.1107/S1744309113011202 ]

Crystallization and preliminary X-ray diffraction analysis of the Cmr2-Cmr3 subcomplex in the CRISPR-Cas RNA-silencing effector complex

T. Osawa, H. Inanaga and T. Numata

Synopsis: The Cmr2-Cmr3 subcomplex from P. furiosus was co-crystallized with 3'-AMP. X-ray diffraction data for the crystals were collected to 2.6 Å resolution using a synchrotron-radiation source.

Online 30 April 2013


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Acta Cryst. (2013). F69, 588-591  [ doi:10.1107/S174430911301110X ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of Bifidobacterium adolescentis xylose isomerase

C. V. dos Reis, A. Bernardes and I. Polikarpov

Synopsis: The xylose isomerase enzyme from B. adolescentis was expressed in E. coli, purified and crystallized. A native X-ray diffraction data set was collected to 1.7 Å resolution.

Online 30 April 2013


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