Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 69, Part 5 (May 2013)

crystallization communications

Acta Cryst. (2013). F69, 540-543    [ doi:10.1107/S1744309113008051 ]

Crystallization and initial X-ray data of abscisic acid receptor PYL3 in the presence of (-)-ABA

X. Zhang, Q. Zhang and G. Wang

Abstract: Abscisic acid (ABA) modulates many complicated developmental processes and responses to environmental stimuli. Recently, several (+)-ABA signalling mechanisms by the RCAR/PYR1/PYL family of proteins (PYLs) have been proposed. However, the mechanism of the recognition and binding of the unnatural ligand (-)-ABA by PYLs has not yet been elucidated. In the present study, the expression, purification and crystallization of PYL3 in complex with (-)-ABA are reported. Diffraction data were refined to 2.65 Å resolution for this complex in space group P65. These findings will help to explain the stereospecificity of PYLs for (-)-ABA and to explore the selective ABA agonists.

Keywords: abscisic acid receptor; (-)-ABA; PYL3.

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