Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 69, Part 6 (June 2013)

structural communications

Acta Cryst. (2013). F69, 618-623    [ doi:10.1107/S1744309113012931 ]

Structure of PatF from Prochloron didemni

A. F. Bent, J. Koehnke, W. E. Houssen, M. C. M. Smith, M. Jaspars and J. H. Naismith

Abstract: Patellamides are macrocyclic peptides with potent biological effects and are a subset of the cyanobactins. Cyanobactins are natural products that are produced by a series of enzymatic transformations and a common modification is the addition of a prenyl group. Puzzlingly, the pathway for patellamides in Prochloron didemni contains a gene, patF, with homology to prenylases, but patellamides are not themselves prenylated. The structure of the protein PatF was cloned, expressed, purified and determined. Prenylase activity could not be demonstrated for the protein, and examination of the structure revealed changes in side-chain identity at the active site. It is suggested that these changes have inactivated the protein. Attempts to mutate these residues led to unfolded protein.

PDB reference: 4bg2

Keywords: patellamide; cyanobactins; natural products; prenyltransferases.

tifdisplay filedownload file

Tagged Image Format File (TIF) image
[ doi:10.1107/S1744309113012931/gx5216sup1.tif ]
Full sequence alignment of PatF and related cyanobactin family members.


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