Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 69, Part 6 (June 2013)


structural communications



Acta Cryst. (2013). F69, 618-623    [ doi:10.1107/S1744309113012931 ]

Structure of PatF from Prochloron didemni

A. F. Bent, J. Koehnke, W. E. Houssen, M. C. M. Smith, M. Jaspars and J. H. Naismith

Abstract: Patellamides are macrocyclic peptides with potent biological effects and are a subset of the cyanobactins. Cyanobactins are natural products that are produced by a series of enzymatic transformations and a common modification is the addition of a prenyl group. Puzzlingly, the pathway for patellamides in Prochloron didemni contains a gene, patF, with homology to prenylases, but patellamides are not themselves prenylated. The structure of the protein PatF was cloned, expressed, purified and determined. Prenylase activity could not be demonstrated for the protein, and examination of the structure revealed changes in side-chain identity at the active site. It is suggested that these changes have inactivated the protein. Attempts to mutate these residues led to unfolded protein.

PDB reference: 4bg2

Keywords: patellamide; cyanobactins; natural products; prenyltransferases.


tifdisplay filedownload file

Tagged Image Format File (TIF) image (434.2 kbytes)
[ doi:10.1107/S1744309113012931/gx5216sup1.tif ]
Full sequence alignment of PatF and related cyanobactin family members.


Notes:

To open or display or play some files, you may need to set your browser up to use the appropriate software. See the full list of file types for an explanation of the different file types and their related mime types and, where available links to sites from where the appropriate software may be obtained.

The download button will force most browsers to prompt for a file name to store the data on your hard disk.

Where possible, images are represented by thumbnails.

 bibliographic record in  format

  Find reference:   Volume   Page   
  Search:     From   to      Advanced search

Copyright © International Union of Crystallography
IUCr Webmaster