Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 69, Part 6 (June 2013)

structural communications

Acta Cryst. (2013). F69, 634-639    [ doi:10.1107/S1744309113013018 ]

Structure of an A-form RNA duplex obtained by degradation of 6S RNA in a crystallization droplet

J. Kondo, A.-C. Dock-Bregeon, D. K. Willkomm, R. K. Hartmann and E. Westhof

Abstract: In the course of a crystallographic study of a 132 nt variant of Aquifex aeolicus 6S RNA, a crystal structure of an A-form RNA duplex containing 12 base pairs was solved at a resolution of 2.6 Å. In fact, the RNA duplex is part of the 6S RNA and was obtained by accidental but precise degradation of the 6S RNA in a crystallization droplet. 6S RNA degradation was confirmed by microscopic observation of crystals and gel electrophoresis of crystallization droplets. The RNA oligomers obtained form regular A-form duplexes containing three GoU wobble-type base pairs, one of which engages in intermolecular contacts through a ribose-zipper motif at the crystal-packing interface.

PDB reference: 4jrt

Keywords: A-form RNA; 6S RNA; RNA degradation.

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[ doi:10.1107/S1744309113013018/tz5032sup1.pdf ]
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