Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 69, Part 7 (July 2013)



[Issue Author Index][Volume Author Index]
[Cover illustration] Cover illustration: Structure of Escherichia coli AdhP (ethanol-inducible dehydrogenase) with bound NAD (Thomas et al., p. 730).

editorial


 

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Acta Cryst. (2013). F69, 711  [ doi:10.1107/S1744309113017909 ]

The future of crystallization communications in Acta Cryst. F

M. S. Weiss and H. Einspahr

Synopsis: Editorial.

Online 30 June 2013


scientific comment


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Acta Cryst. (2013). F69, 712-718  [ doi:10.1107/S1744309113014152 ]

Crystallization reports are the backbone of Acta Cryst. F, but do they have any spine?

J. Newman, D. R. Burton, S. Caria, S. Desbois, C. L. Gee, V. J. Fazio, M. Kvansakul, B. Marshall, G. Mills, V. Richter, S. A. Seabrook, M. Wu and T. S. Peat

Synopsis: All the crystallization communications published in Acta Cryst. F in 2012 were analysed. Details of the analysis are presented, along with some suggestions for making this type of publication more useful.

Online 30 June 2013


structural communications


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Acta Cryst. (2013). F69, 719-722  [ doi:10.1107/S1744309113014036 ]

Structure of peroxiredoxin from the anaerobic hyperthermophilic archaeon Pyrococcus horikoshii

T. Nakamura, A. Mori, M. Niiyama, H. Matsumura, C. Tokuyama, J. Morita, K. Uegaki and T. Inoue

Synopsis: The crystal structure of the archaeal peroxiredoxin from P. horikoshii was determined at a resolution of 2.25 Å and compared with that of a homologous protein from A. pernix.

PDB reference: 3w6g

Online 27 June 2013


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Acta Cryst. (2013). F69, 723-729  [ doi:10.1107/S1744309113015182 ]

The structure of the TOG-like domain of Drosophila melanogaster Mast/Orbit

T. De la Mora-Rey, B. D. Guenther and B. C. Finzel

Synopsis: The crystal structure of Mast-M1 has been determined at 2.0 Å resolution and provides the first detailed structural description of a TOG-like domain.

PDB reference: 4g3a

Online 27 June 2013


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Acta Cryst. (2013). F69, 730-732  [ doi:10.1107/S1744309113015170 ]

Structure of Escherichia coli AdhP (ethanol-inducible dehydrogenase) with bound NAD

L. M. Thomas, A. R. Harper, W. A. Miner, H. O. Ajufo, K. M. Branscum, L. Kao and P. A. Sims

Synopsis: The crystal structure of E. coli AdhP (ethanol-inducible dehydrogenase) in complex with NAD is reported.

PDB reference: 4gkv

Online 27 June 2013


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Acta Cryst. (2013). F69, 733-737  [ doi:10.1107/S174430911301614X ]

Structure of a family 3a carbohydrate-binding module from the cellulosomal scaffoldin CipA of Clostridium thermocellum with flanking linkers: implications for cellulosome structure

O. Yaniv, E. Morag, I. Borovok, E. A. Bayer, R. Lamed, F. Frolow and L. J. W. Shimon

Synopsis: The structure of the cellulose-binding module of the C. thermocellum cellulosome-integrating protein A scaffoldin with portions of the C- and N-terminal flanking linker regions has been determined at 1.98 Å resolution.

PDB reference: 4jo5

Online 27 June 2013


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Acta Cryst. (2013). F69, 738-743  [ doi:10.1107/S1744309113016539 ]

Structures of MauG in complex with quinol and quinone MADH

E. T. Yukl, L. M. R. Jensen, V. L. Davidson and C. M. Wilmot

Synopsis: Crystal structures of MauG in complexes with MADH with its TTQ cofactor in the quinone and quinol forms are reported.

PDB references: 3sws, 3sxt and 4k3i

Online 27 June 2013


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Acta Cryst. (2013). F69, 744-752  [ doi:10.1107/S1744309113014206 ]

High-resolution structure of the Tiam1 PHn-CC-Ex domain

M. Joshi, L. Gakhar and E. J. Fuentes

Synopsis: The structures of two Tiam1 PHn-CC-Ex domain variants have been determined to resolutions of 1.98 and 2.15 Å. Biochemical data indicate that this domain binds the coiled-coil domain of Par3b. SAXS data are in excellent agreement with the structures, indicating that the solution and crystal structures are very similar.

PDB references: 4k2o and 4k2p

Online 27 June 2013


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Acta Cryst. (2013). F69, 753-758  [ doi:10.1107/S1744309113013468 ]

Conformational stability and crystal packing: polymorphism in Neurospora crassa CAT-3

A. Zárate-Romero, V. Stojanoff, S. P. Rojas-Trejo, W. Hansberg and E. Rudiño-Piñera

Synopsis: Comparisons between polymorphic structures in space groups P1, P212121 and P43212 highlight the conformational stability of tetrameric CAT-3 and reveal a distortion in the tetrameric structure that has not previously been described.

PDB references: 3zj4, 3zj5 and 4bim

Online 27 June 2013


crystallization communications


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Acta Cryst. (2013). F69, 759-761  [ doi:10.1107/S1744309113012487 ]

Purification, crystallization and preliminary X-ray crystallographic study of the tRNA pseudouridine synthase TruB from Streptococcus pneumoniae

W. Yang, S. Zhao, L. Jin, Z. Guo, S. Zhang, H. Zhang and D. Wang

Synopsis: The RNA pseudouridine synthase TruB catalyses the isomerization of uridine to pseudouridine at residue 55 of elongator tRNAs. Here, the expression, purification, crystallization and preliminary crystallographic analysis of TruB from Streptococcus pneumoniae are reported.

Online 28 June 2013


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Acta Cryst. (2013). F69, 762-765  [ doi:10.1107/S1744309113014012 ]

Protein expression, crystallization and preliminary X-ray crystallographic analysis of the isolated Shigella flexneri VapC toxin

K. Xu, E. Dedic, P. Cob-Cantal, C. Dienemann, A. Bøggild, K. S. Winther, K. Gerdes and D. E. Brodersen

Synopsis: The purification and crystallization of the isolated toxin VapC (MvpT) from the VapBC toxin-antitoxin complex of the pathogen S. flexneri are reported. The crystals belonged to space group H3 and diffracted to 1.9 Å resolution.

Online 28 June 2013


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Acta Cryst. (2013). F69, 766-770  [ doi:10.1107/S174430911301419X ]

Crystallization and X-ray diffraction analysis of the N-terminal domain of the Toll-like receptor signalling adaptor protein TRIF/TICAM-1

M. O. Ullah, T. Ve, J. Dkhar, M. Alaidarous, D. J. Ericsson, M. J. Sweet, A. Mansell and B. Kobe

Synopsis: This report describes the crystallization and X-ray diffraction analysis of a regulatory domain of the Toll-like receptor signalling adaptor TRIF/TICAM-1 and its SeMet-labelled mutant containing two additional Met residues. This domain is unrelated in sequence to any protein of known structure.

Online 28 June 2013


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Acta Cryst. (2013). F69, 771-775  [ doi:10.1107/S1744309113014863 ]

Expression, derivatization, crystallization and experimental phasing of an extracellular segment of the human Robo1 receptor

R. Barak and Y. Opatowsky

Synopsis: Robo receptors participate in the orchestration of several developmental responses, most notably axonal guidance in the central nervous system (CNS). Here, the expression, derivatization, crystallization, and experimental phasing of the extracellular juxtamembrane segment of the human Robo1 receptor are described.

Online 28 June 2013


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Acta Cryst. (2013). F69, 776-778  [ doi:10.1107/S174430911301498X ]

Crystallization and preliminary X-ray diffraction analysis of a thermostable GDSL-family esterase, EstL5, from Geobacillus thermodenitrificans T2

Z. Yang, Y. Zhang, R. Qiu, J. Huang and C. Ji

Synopsis: As a thermostable esterase with cold adaptation, EstL5 might be of significant industrial interest and value in scientific research. In order to provide new insights into the structure-function relationship of EstL5 and to help better understand the biochemical data, a project to determine the three-dimensional structure of this enzyme was initiated.

Online 28 June 2013


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Acta Cryst. (2013). F69, 779-782  [ doi:10.1107/S1744309113015030 ]

The mannose-specific lectin domains of Flo1p from Saccharomyces cerevisiae and Lg-Flo1p from S. pastorianus: crystallization and preliminary X-ray diffraction analysis of the adhesin-carbohydrate complexes

F. S. Ielasi, P. Goyal, M. Sleutel, A. Wohlkonig and R. G. Willaert

Synopsis: The N-terminal carbohydrate-binding domains of Flo1p and Lg-Flo1p from two different Saccharomyces spp. were overexpressed in E. coli, purified and crystallized in different conditions. A preliminary X-ray analysis of the crystallized flocculin-protein complexes is reported in this work.

Online 28 June 2013


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Acta Cryst. (2013). F69, 783-787  [ doi:10.1107/S1744309113015200 ]

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of human histidine triad nucleotide-binding protein 2 (hHINT2)

R. Dolot, A. Wlodarczyk, G. D. Bujacz and B. Nawrot

Synopsis: Human histidine triad nucleotide-binding protein 2 (hHINT2) was cloned, expressed in E. coli, purified and crystallized. The initially obtained crystals of hHINT2 were used for a diffraction experiment and data were collected to 2.83 Å resolution.

Online 28 June 2013


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Acta Cryst. (2013). F69, 788-791  [ doi:10.1107/S174430911301525X ]

Cloning, expression, purification, crystallization and preliminary crystallographic analysis of the kinase domain of AtMAP4Kalpha2 from Arabidopsis thaliana

L. Shen, X. Du, Q. Su, M. Li and Z. Zhou

Synopsis: The kinase domain of AtMAP4Kalpha2 was cloned, expressed, purified and crystallized. The crystals diffracted to 1.9 Å resolution and belonged to space group C2221, with unit-cell parameters a = 55.27, b = 82.93, c = 133.15 Å.

Online 28 June 2013


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Acta Cryst. (2013). F69, 792-795  [ doi:10.1107/S1744309113015583 ]

Crystallization and preliminary crystallographic studies of GRASP65 GRASP domain from Rattus norvegicus

X. Li, Y. Feng and X. Liu

Synopsis: The GRASP65 GRASP domain from R. norvegicus has been expressed, purified and crystallized. The crystals belonged to space group P21212, with unit-cell parameters a = 44.99, b = 104.29, c = 37.93 Å, and diffracted to 2.0 Å resolution.

Online 28 June 2013


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Acta Cryst. (2013). F69, 796-799  [ doi:10.1107/S1744309113016710 ]

X-ray diffraction studies on merohedrally twinned [Delta]1-62NtNBCe1-A crystals of the sodium/bicarbonate cotransporter

H. S. Gill, L. Dutcher, W. F. Boron, S. Patel and L. M. Guay-Woodford

Synopsis: A truncated mutant missing the first 62 residues of the N-terminal, cytoplasmic domain of the sodium-bicarbonate NBCe1-A cotransporter crystallizes in space group P31 with pseudo-P3121 symmetry and a hemihedral twin fraction of 33.0%. Twinned fractions and twin-pair statistics over binned resolutions confirm that the calculated twin fraction is associated with hemihedral twinning and not to non-crystallographic symmetry.

Online 28 June 2013


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Acta Cryst. (2013). F69, 800-803  [ doi:10.1107/S1744309113015613 ]

Purification, crystallization and preliminary X-ray analysis of the AAA+ [sigma]54 activator domain of FlrC from Vibrio cholerae

S. Dey and J. Dasgupta

Synopsis: The AAA+ [sigma]54 interaction domain of FlrC from V. cholerae has been cloned, overexpressed, purified and crystallized in a heptameric form. Crystals belonging to space group P212121 produced diffraction data to 2.8 Å resolution.

Online 30 June 2013


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Acta Cryst. (2013). F69, 804-808  [ doi:10.1107/S1744309113016266 ]

Preliminary crystallographic analysis of the N-terminal PDZ-like domain of periaxin, an abundant peripheral nerve protein linked to human neuropathies

H. Han and P. Kursula

Synopsis: The N-terminal PDZ-like domain of the human peripheral nervous system myelin protein periaxin has been crystallized and preliminary diffraction data are reported. The periaxin crystals diffracted X-rays to 2.85 Å resolution using synchrotron radiation.

Online 30 June 2013


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Acta Cryst. (2013). F69, 809-811  [ doi:10.1107/S1744309113016692 ]

Expression, crystallization and preliminary X-ray analysis of rice L-galactose dehydrogenase

M. Momma and Z. Fujimoto

Synopsis: Rice L-galactose dehydrogenase was crystallized and X-ray diffraction data were collected to 1.2 Å resolution.

Online 30 June 2013


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Acta Cryst. (2013). F69, 812-814  [ doi:10.1107/S1744309113016709 ]

Crystallization and preliminary X-ray study of the deaminase AmnE from Pseudomonas sp. AP-3

D. Yu, Y. Jiang, J. Hou, S. Chen, G. Zhang, X. Liu, H. Dong and B. Yu

Synopsis: The deaminase AmnE from Pseudomonas sp. AP-3 was expressed in E. coli and purified. Crystallization and preliminary X-ray crystallographic analysis were performed for this recombinant enzyme.

Online 30 June 2013


laboratory communications


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Acta Cryst. (2013). F69, 815-820  [ doi:10.1107/S1744309113014425 ]

Mimer: an automated spreadsheet-based crystallization screening system

D. E. Brodersen, G. R. Andersen and C. B. F. Andersen

Synopsis: An automated spreadsheet-based solution for macromolecular crystallization screening is presented that allows easy formulation and optimization of crystal screens via multiple approaches, including incomplete-factorial and grid screening.

Online 29 June 2013


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Acta Cryst. (2013). F69, 821-826  [ doi:10.1107/S1744309113013651 ]

A strategy for selecting the pH of protein solutions to enhance crystallization

C.-Y. Zhang, Z.-Q. Wu, D.-C. Yin, B.-R. Zhou, Y.-Z. Guo, H.-M. Lu, R.-B. Zhou and P. Shang

Synopsis: The pH of a solution is an important parameter in crystallization that needs to be controlled in order to ensure success. In this study, the effects of the buffer and protein solution pH values on the results of screening are systematically investigated.

Online 29 June 2013


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