Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 69, Part 9 (September 2013)


structural communications



Acta Cryst. (2013). F69, 979-988    [ doi:10.1107/S1744309113021799 ]

Structure of the Aeropyrum pernix L7Ae multifunctional protein and insight into its extreme thermostability

M. W. Bhuiya, J. Suryadi, Z. Zhou and B. A. Brown II

Abstract: Archaeal ribosomal protein L7Ae is a multifunctional RNA-binding protein that directs post-transcriptional modification of archaeal RNAs. The L7Ae protein from Aeropyrum pernix (Ap L7Ae), a member of the Crenarchaea, was found to have an extremely high melting temperature (>383 K). The crystal structure of Ap L7Ae has been determined to a resolution of 1.56 Å. The structure of Ap L7Ae was compared with the structures of two homologs: hyperthermophilic Methanocaldococcus jannaschii L7Ae and the mesophilic counterpart mammalian 15.5 kD protein. The primary stabilizing feature in the Ap L7Ae protein appears to be the large number of ion pairs and extensive ion-pair network that connects secondary-structural elements. To our knowledge, Ap L7Ae is among the most thermostable single-domain monomeric proteins presently observed.

PDB reference: 2fc3

Keywords: Aeropyrum pernix; L7Ae; thermal stability; ion pairs; ribosomal proteins.


pdfdisplay filedownload file

Portable Document Format (PDF) file
[ doi:10.1107/S1744309113021799/be5231sup1.pdf ]
Supplementary material


Notes:

To open or display or play some files, you may need to set your browser up to use the appropriate software. See the full list of file types for an explanation of the different file types and their related mime types and, where available links to sites from where the appropriate software may be obtained.

The download button will force most browsers to prompt for a file name to store the data on your hard disk.

Where possible, images are represented by thumbnails.

 bibliographic record in  format

  Find reference:   Volume   Page   
  Search:     From   to      Advanced search

Copyright © International Union of Crystallography
IUCr Webmaster