(a) Elution profile of RlmJ from a HiLoad 16/60 Superdex 75 gel-filtration column. The major peak corresponds to monomeric RlmJ. Elution volumes of protein molecular-mass standards are indicated at the top. (b) PhastGel 8–25% gradient SDS–PAGE analysis of RlmJ purification. Lane 1, molecular-weight marker (labelled in kDa); lane 2, cell lysate; lane 3, flowthrough from Ni Sepharose column; lane 4, final wash fraction from Ni Sepharose column; lanes 5–7, elution fractions from Ni Sepharose column; lane 8, final concentrated RlmJ after HiLoad 16/60 Superdex 75 gel-filtration column.