Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 69, Part 10 (October 2013)



[Issue Author Index][Volume Author Index]
[Cover illustration] Cover illustration: Monoclinic casein kinase 1 [delta] (Zeringo et al., p. 1077).

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Acta Cryst. (2013). F69, 1071-1076  [ doi:10.1107/S1744309113014383 ]

Unexpected features in the Protein Data Bank entries 3qd1 and 4i8e: the structural description of the binding of the serine-rich repeat adhesin GspB to host cell carbohydrate receptor is not a solved issue

Y. A. Muller

Synopsis: A recently published crystallographic model of GspB in complex with a disaccharide was proposed to provide insight into the interaction of the S. gordonii adhesin with the cell sialyl-T antigen from host cells. However, a close inspection of this model revealed that in this complex a buffer molecule was mistaken for the disaccharide. As a consequence, this complex provides no information regarding the interaction of GspB with host cell receptors.

Online 28 September 2013


structural communications


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Acta Cryst. (2013). F69, 1077-1083  [ doi:10.1107/S1744309113023403 ]

A monoclinic crystal form of casein kinase 1 [delta]

N. A. Zeringo, L. Murphy, E. A. McCloskey, L. Rohal and J. J. Bellizzi

Synopsis: The crystal structure of a new P21 crystal form of the catalytic domain of mammalian casein kinase 1 [delta] is described and compared with previously deposited structures.

PDB reference: 4jjr

Online 28 September 2013


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Acta Cryst. (2013). F69, 1084-1088  [ doi:10.1107/S1744309113023993 ]

Structure of 2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase from Pseudomonas aeruginosa

S. K. Nelson, A. Kelleher, G. Robinson, S. Reiling and O. A. Asojo

Synopsis: The recombinant production, purification, crystallization and crystal structure of 2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase from P. aeruginosa is presented.

PDB reference: 4lu0

Online 28 September 2013


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Acta Cryst. (2013). F69, 1089-1093  [ doi:10.1107/S1744309113024986 ]

Structure of succinyl-CoA:3-ketoacid CoA transferase from Drosophila melanogaster

M. Zhang, H.-Y. Xu, Y.-C. Wang, Z.-B. Shi and N.-N. Zhang

Synopsis: The crystal structure of succinyl-CoA: 3-ketoacid CoA transferase from Drosophila melanogaster was determined at 2.64 Å resolution.

PDB reference: 4kgb

Online 28 September 2013


crystallization communications


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Acta Cryst. (2013). F69, 1094-1096  [ doi:10.1107/S1744309113023142 ]

Crystallization and preliminary X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1

L. Wan, S. J. Williams, X. Zhang, D. J. Ericsson, M. Koeck, P. N. Dodds, J. G. Ellis and B. Kobe

Synopsis: This report describes the crystallization and X-ray diffraction analysis of the flax cytokinin oxidase LuCKX1.1.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1097-1099  [ doi:10.1107/S1744309113023154 ]

Expression, purification and crystallization of the FP domain of the human F-box protein Fbxo7

J. Shang, G. Wang, Y. Yang, X. Huang and Z. Du

Synopsis: The 155 amino-acid FP domain of the human F-box protein Fbxo7 was successfully expressed in bacteria, purified and crystallized. Native and single-wavelength anomalous dispersion data sets have been collected.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1100-1102  [ doi:10.1107/S1744309113023348 ]

Preliminary X-ray diffraction analysis of thermostable [beta]-1,4-mannanase from Aspergillus niger BK01

W. Luo, J.-W. Huang, C.-H. Huang, T.-Y. Huang, H.-C. Chan, J.-R. Liu, R.-T. Guo and C.-C. Chen

Synopsis: The recombinant protein of acidic thermophilic [beta]-1,4-mannanase from Aspergillus niger BK01 was expressed, purified, and crystallized. X-ray diffraction data to 1.57 Å was collected and reported.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1103-1106  [ doi:10.1107/S1744309113024871 ]

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of SpyCEP, a candidate antigen for a vaccine against Streptococcus pyogenes

F. Abate, E. Malito, F. Falugi, I. Margarit Y Ros and M. J. Bottomley

Synopsis: The cloning, expression and purification of different fragments of the S. pyogenes SpyCEP protein are reported. Preliminary X-ray diffraction analysis of a 1580-residue selenomethionine-labelled ectodomain fragment is described.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1107-1109  [ doi:10.1107/S1744309113023439 ]

Purification, crystallization and preliminary crystallographic studies of C-terminal RNA recognition motif (RRM-3) of human ELAV-type RNA-binding protein 3 (ETR-3)

M. Kashyap, A. Sharma and N. S. Bhavesh

Synopsis: The C-terminal RNA recognition motif of human ETR-3 protein involved in myotonic dystrophy was over-expressed, purified and crystallized. The crystals obtained diffracted X-rays to 3 Å resolution and belonged to space group P213.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1110-1113  [ doi:10.1107/S1744309113023452 ]

Cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of Rv0241c (HtdX) from Mycobacterium tuberculosis H37Rv

R. Biswas, D. Dutta and A. K. Das

Synopsis: A putative (3R)-hydroxyacyl-CoA dehydratase, HtdX from M. tuberculosis H37Rv, has been cloned, overexpressed, purified and crystallized to collect preliminary X-ray diffraction data.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1114-1119  [ doi:10.1107/S1744309113023609 ]

Crystallization and preliminary crystallographic analysis of GanB, a GH42 intracellular [beta]-galactosidase from Geobacillus stearothermophilus

H. V. Solomon, O. Tabachnikov, H. Feinberg, L. Govada, N. E. Chayen, Y. Shoham and G. Shoham

Synopsis: The GH42 [beta]-galactosidase GanB from G. stearothermophilus has been crystallized in the primitive orthorhombic space group P212121. Complete diffraction data sets have been measured for the wild-type enzyme (2.45 Å resolution) and its catalytic mutant (E323A; 2.50 Å resolution) for use in a full three-dimensional structural analysis of the GanB protein.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1120-1122  [ doi:10.1107/S1744309113023506 ]

Expression, crystallization and preliminary X-ray crystallographic analysis of peptide deformylase from Campylobacter jejuni

H. T. Tran, T.-V. Pham, H.-P.-T. Ngo, M. Hong, Y.-J. Ahn and L.-W. Kang

Synopsis: Peptide deformylase catalyzes the deformylation of N-formylated methionine in newly synthesized polypeptides in prokaryotes and some eukaryotic organelles. The synthetic codon-optimized def gene from C. jejuni was cloned and the protein expressed, purified and crystallized. A preliminary X-ray crystallography analysis of the C. jejuni peptide deformylase crystals was performed.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1123-1126  [ doi:10.1107/S1744309113023828 ]

Identification, characterization and preliminary X-ray diffraction analysis of the rolling-circle replication initiator protein from plasmid pSTK1

S. B. Carr, L. B. Mecia, S. E. V. Phillips and C. D. Thomas

Synopsis: A proteolytically stable fragment of a plasmid replication initiation protein from the thermophile G. stearothermophilus has been biochemically characterized, crystallized and diffraction data collected to a resolution of 2.5 Å.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1127-1130  [ doi:10.1107/S1744309113023877 ]

Overexpression, crystallization and preliminary X-ray crystallographic analysis of a putative xylose isomerase from Bacteroides thetaiotaomicron

J.-W. Cho, B.-G. Han, S. Y. Park, S. J. Kim, M.-D. Kim and B. I. Lee

Synopsis: A putative xylose isomerase from B. thetaiotaomicron has been crystallized (space group P1, unit-cell parameters a = 96.3, b = 101.7, c = 108.3 Å, [alpha] = 82.8, [beta] = 68.2, [gamma] = 83.0°). Diffraction data have been collected to 2.10 Å resolution using synchrotron X-rays.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1131-1134  [ doi:10.1107/S1744309113023956 ]

Crystallization and preliminary X-ray diffraction studies of D-threo-3-hydroxyaspartate dehydratase isolated from Delftia sp. HT23

Y. Matsumoto, Y. Yasutake, Y. Takeda, T. Tamura, A. Yokota and M. Wada

Synopsis: Improvement of the expression level, crystallization and preliminary X-ray diffraction studies of D-threo-3-hydroxyaspartate dehydratase isolated from Delftia sp. HT23 are reported.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1135-1139  [ doi:10.1107/S174430911302397X ]

Crystallization of the C-terminal head domain of the fibre protein from a siadenovirus, turkey adenovirus 3

A. K. Singh, M. Z. Ballmann, M. Benko, B. Harrach and M. J. van Raaij

Synopsis: The C-terminal domain of the fibre protein from turkey adenovirus 3, a siadenovirus, consisting of amino acids 304-454, has been crystallized. Diffraction data were obtained to 2.0 and 2.14 Å resolution for a native crystal and a selenomethionine-derivative crystal, respectively.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1140-1142  [ doi:10.1107/S174430911302410X ]

Crystallization and preliminary X-ray study of a (2R,3R)-2,3-butanediol dehydrogenase from Bacillus coagulans 2-6

X. Miao, X. Huang, G. Zhang, X. Zhao, X. Zhu and H. Dong

Synopsis: A (2R,3R)-2,3-butanediol dehydrogenase from B. coagulans 2-6 was expressed in E. coli BL21 (DE3) and purified. Crystallization and preliminary X-ray crystallographic analysis were performed for this recombinant enzyme.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1143-1146  [ doi:10.1107/S1744309113024172 ]

Crystallization and preliminary X-ray diffraction analysis of a high-affinity phosphate-binding protein endowed with phosphatase activity from Pseudomonas aeruginosa PAO1

A. Djeghader, G. Gotthard, A. Suh, D. Gonzalez, K. Scott, E. Chabriere and M. Elias

Synopsis: A phosphate-binding protein endowed with phosphatase activity, previously dubbed LapA, from P. aeruginosa PAO1 has been crystallized. The crystals diffracted to 0.87 Å resolution and belonged to space group P21.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1147-1150  [ doi:10.1107/S1744309113024184 ]

Crystallization and preliminary crystallographic analysis of a viral RNA-silencing suppressor encoded by Wuhan nodavirus

Y. Liu, Y. Zhao, Y. Zhang and H. Chen

Synopsis: A viral RNA-silencing suppressor encoded by Wuhan nodavirus has been overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.8 Å resolution.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1151-1154  [ doi:10.1107/S1744309113024263 ]

Crystallization and X-ray diffraction analysis of nylon hydrolase (NylC) from Arthrobacter sp. KI72

K. Nagai, K. Yasuhira, Y. Tanaka, D. Kato, M. Takeo, Y. Higuchi, S. Negoro and N. Shibata

Synopsis: Diffraction data were collected from a NylCp2 crystal to a resolution of 1.60 Å. The crystal belonged to space group C2221, with unit-cell parameters a = 70.84, b = 144.90, c = 129.05 Å.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1155-1158  [ doi:10.1107/S1744309113024330 ]

Expression, purification and preliminary X-ray crystallographic analysis of Arf1-GDP in complex with dimeric p23 peptide

P. Zheng, F. Gao, K. Deng, W. Gong and Z. Sun

Synopsis: The complex of Arf1-GDP and dimeric p23 peptide, which is likely to be involved in membrane binding of Arf1, has been crystallized and preliminary crystallographic analysis was performed.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1159-1162  [ doi:10.1107/S1744309113024366 ]

Purification, crystallization and preliminary X-ray crystallographic analysis of the transport unit of the monomeric autotransporter AIDA-I from Escherichia coli

I. Gawarzewski, B. Tschapek, A. Hoeppner, J. Jose, S. H. J. Smits and L. Schmitt

Synopsis: Here, the purification, crystallization and preliminary X-ray crystallographic studies of the transport unit of AIDA-I are reported.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1163-1166  [ doi:10.1107/S1744309113024391 ]

Expression, crystallization and preliminary X-ray crystallographic analysis of cellobiose 2-epimerase from Dictyoglomus turgidum DSM 6724

T.-V. Pham, S.-H. Hong, M.- Hong, H.-P.-T. Ngo, D.-K. Oh and L.-W. Kang

Synopsis: Cellobiose 2-epimerase epimerizes and isomerizes [beta]-1,4- and [alpha]-1,4-gluco-oligosaccharides. The cellobiose 2-epimerase gene from D. turgidum was cloned and the protein was expressed, purified and crystallized. A preliminary X-ray crystallography analysis of the cellobiose 2-epimerase crystals was performed.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1167-1170  [ doi:10.1107/S1744309113024408 ]

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of the TIR domain from the Brucella melitensis TIR-domain-containing protein TcpB

M. Alaidarous, T. Ve, M. O. Ullah, E. Valkov, A. Mansell, M. A. Schembri, M. J. Sweet and B. Kobe

Synopsis: In this study, the B. melitensis TIR-domain-containing protein (TcpB) was cloned, expressed, purified and crystallized, and X-ray diffraction data were collected to 2.6 Å resolution.

Online 28 September 2013


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Acta Cryst. (2013). F69, 1171-1176  [ doi:10.1107/S174430911302441X ]

Crystallization and preliminary structural characterization of the two actin isoforms of the malaria parasite

S. P. Bhargav, J. Vahokoski, E.-P. Kumpula and I. Kursula

Synopsis: The two Plasmodium actin isoforms were expressed, purified and crystallized in complex with the gelsolin G1 domain. Crystals diffracting to high resolution were obtained and data sets for actin I and II were collected to 1.19 and 2.2 Å resolution, respectively.

Online 30 September 2013


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Acta Cryst. (2013). F69, 1177-1181  [ doi:10.1107/S1744309113024639 ]

Cloning to crystallization of dihydrodipicolinate synthase from the intracellular pathogen Legionella pneumophila

T. Siddiqui, J. J. Paxman, C. Dogovski, S. Panjikar and M. A. Perugini

Synopsis: Dihydrodipicolinate synthase (DHDPS) catalyses the rate-limiting step in the biosynthesis of meso-diaminopimelate and lysine. Here, the cloning, expression, purification and crystallization of DHDPS from the intracellular pathogen Legionella pneumophila are described.

Online 30 September 2013


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Acta Cryst. (2013). F69, 1182-1185  [ doi:10.1107/S1744309113024676 ]

Purification, crystallization and preliminary crystallographic analysis of the SpaA backbone-pilin subunit from probiotic Lactobacillus rhamnosus GG

D. Singh, I. von Ossowski, A. Palva and V. Krishnan

Synopsis: SpaA pilin, which forms the pilus shaft of SpaCBA pili in L. rhamnosus GG, was crystallized and X-ray diffraction data were collected to a resolution of 2.0 Å.

Online 30 September 2013


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Acta Cryst. (2013). F69, 1186-1189  [ doi:10.1107/S1744309113024810 ]

Crystallization and preliminary X-ray diffraction studies of Tyr167His mutant [alpha]-cyclodextrin glucanotransferase from Bacillus macerans

Y. Yue, S. Liu, H. Li, B. Song, T. Xie, Y. Sun, Y. Chao and S. Qian

Synopsis: The constructed Y167H mutant [alpha]-cyclodextrin glucanotransferase was successfully expressed and purified. Single crystals were grown with two kinds of morphology in different crystallization conditions.

Online 30 September 2013


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