Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 69, Part 11 (November 2013)



[Issue Author Index][Volume Author Index]
[Cover illustration] Cover illustration: Whirly protein WHY1 from Arabidopsis thaliana (Cappadocia et al., p. 1207).

structural communications


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Acta Cryst. (2013). F69, 1190-1195  [ doi:10.1107/S1744309113026857 ]

Structures of Saccharomyces cerevisiae D-arabinose dehydrogenase Ara1 and its complex with NADPH: implications for cofactor-assisted substrate recognition

X.-Q. Hu, P.-C. Guo, J.-D. Ma and W.-F. Li

Synopsis: Here, crystal structures of Ara1 in apo and NADPH-complexed forms are presented at 2.10 and 2.00 Å resolution, respectively.

PDB references: 4ijc and 4ijr

Online 26 October 2013


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Acta Cryst. (2013). F69, 1196-1201  [ doi:10.1107/S1744309113027371 ]

Structure of CT584 from Chlamydia trachomatis refined to 3.05 Å resolution

M. L. Barta, J. Hickey, K. E. Kemege, S. Lovell, K. P. Battaile and P. S. Hefty

Synopsis: The crystal structure of CT584 from C. trachomatis is presented at 3.05 Å resolution.

PDB reference: 4mlk

Online 26 October 2013


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Acta Cryst. (2013). F69, 1202-1206  [ doi:10.1107/S1744309113028170 ]

The tertiary structure of an i-type lysozyme isolated from the common orient clam (Meretrix lusoria)

Y. Kuwano, K. Yoneda, Y. Kawaguchi and T. Araki

Synopsis: The tertiary structure of invertebrate-type lysozyme from the common orient clam was determined and compared with that of V. philippinarum lysozyme.

PDB reference: 3ab6

Online 26 October 2013


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Acta Cryst. (2013). F69, 1207-1211  [ doi:10.1107/S1744309113028698 ]

A family portrait: structural comparison of the Whirly proteins from Arabidopsis thaliana and Solanum tuberosum

L. Cappadocia, J.-S. Parent, J. Sygusch and N. Brisson

Synopsis: The crystallization and crystal structure determination of the three Whirly proteins from A. thaliana is described.

PDB references: 4koo, 4kop and 4koq

Online 26 October 2013


crystallization communications


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Acta Cryst. (2013). F69, 1212-1215  [ doi:10.1107/S1744309113025761 ]

Crystallization and X-ray crystallographic analysis of the cholesterol-dependent cytolysin listeriolysin O from Listeria monocytogenes

S. Köster, M. Hudel, T. Chakraborty and Ö. Yildiz

Synopsis: The cholesterol-dependent cytolysin listeriolysin O from L. monocytogenes has been purified and crystallized. The orthorhombic crystals diffracted to beyond 2.2 Å resolution and contained one molecule in the asymmetric unit.

Online 17 October 2013


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Acta Cryst. (2013). F69, 1216-1219  [ doi:10.1107/S1744309113025803 ]

Crystallization and preliminary X-ray diffraction studies of Staphylococcus aureus homoserine dehydrogenase

V. Navratna and B. Gopal

Synopsis: The cloning, purification, crystallization and preliminary X-ray diffraction studies of S. aureus homoserine dehydrogenase, an enzyme that regulates the biosynthesis of several essential amino acids, are reported.

Online 17 October 2013


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Acta Cryst. (2013). F69, 1220-1223  [ doi:10.1107/S1744309113025578 ]

Purification, crystallization and preliminary crystallographic analysis of the globular domain of the human type V myosin Myo5a

H. Velvarska and D. Niessing

Synopsis: The globular cargo-binding domain of human Myo5a was cloned, expressed, purified and crystallized and the crystals were characterized. Special precautions that were necessary to harvest diffraction-quality crystals are described.

Online 17 October 2013


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Acta Cryst. (2013). F69, 1224-1226  [ doi:10.1107/S1744309113025566 ]

GPX3 from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis

K. Li, Q. Yang, W. Wang, X. Zhao and Z. Lou

Synopsis: The cytoplasmic domain of glutathione peroxidase 3 (GPX3) from A. thaliana was overexpressed in E. coli, purified and crystallized. Diffraction data were collected to a resolution of 2.8 Å.

Online 17 October 2013


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Acta Cryst. (2013). F69, 1227-1230  [ doi:10.1107/S1744309113025426 ]

Crystallization and preliminary X-ray study of alanine dehydrogenase from Bacillus pseudofirmus OF4

J. Wen, Z. Li, G. He, S. Xu, B. Zhao, X. Zhu, H. Dong and J. Ju

Synopsis: An alanine dehydrogenase from B. pseudofirmus OF4 was expressed in E. coli and purified. Crystallization and preliminary X-ray crystallographic analysis of the recombinant enzyme were performed.

Online 17 October 2013


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Acta Cryst. (2013). F69, 1231-1234  [ doi:10.1107/S1744309113024858 ]

Crystallization and preliminary X-ray diffraction of the RNA demethylase ALKBH5

B. Zhou and Z. Han

Synopsis: ALKBH5 catalyzes the demethylation of N6-methyladenosine in RNA. The ALKBH5 protein was crystallized and X-ray diffraction data were collected to a resolution of 2.4 Å.

Online 17 October 2013


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Acta Cryst. (2013). F69, 1235-1238  [ doi:10.1107/S1744309113024846 ]

Crystallization and preliminary X-ray diffraction analysis of the lactonase VmoLac from Vulcanisaeta moutnovskia

J. Hiblot, G. Gotthard, C. Champion, E. Chabriere and M. Elias

Synopsis: A phosphotriesterase-like lactonase dubbed VmoLac isolated from the hyperthermophilic crenarchaeon V. moutnovskia was purified and crystallized. A diffraction data set was collected to 2.4 Å resolution.

Online 17 October 2013


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Acta Cryst. (2013). F69, 1239-1241  [ doi:10.1107/S174430911302486X ]

12-Fold symmetry of the putative portal protein from the Thermus thermophilus bacteriophage G20C determined by X-ray analysis

L. S. Williams, V. M. Levdikov, L. Minakhin, K. Severinov and A. A. Antson

Synopsis: Crystal data on a putative portal protein from the thermostable bacteriophage G20C indicate that it forms a 12-subunit assembly.

Online 17 October 2013


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Acta Cryst. (2013). F69, 1242-1245  [ doi:10.1107/S1744309113024822 ]

Preliminary crystallographic analysis of recombinant VapBC-15 toxin-antitoxin complex from Mycobacterium tuberculosis

U. Das, N. Kumar, S. Gourinath and A. Srinivasan

Synopsis: The VapBC-15 toxin-antitoxin protein complex was purified and crystallized using the vapour-diffusion technique. A diffraction data set was collected to 2.6 Å resolution.

Online 17 October 2013


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Acta Cryst. (2013). F69, 1246-1248  [ doi:10.1107/S1744309113026018 ]

Cloning, purification, crystallization and preliminary X-ray studies of the putative transcriptional regulator SPD0280 from Streptococcus pneumoniae D39

S. Zhang, X. Min, Z. Guo, H. Zhang, A. Huang, Y. Yin and D. Wang

Synopsis: The cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the putative transcriptional regulator SPD0280 from S. pneumoniae D39 are described.

Online 19 October 2013


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Acta Cryst. (2013). F69, 1249-1251  [ doi:10.1107/S174430911302472X ]

Cloning, overexpression, purification and crystallization of malate dehydrogenase from Thermus thermophilus

Y.-Y. Chang, C.-H. Hung, T.-S. Hwang and C.-H. Hsu

Synopsis: The malate dehydrogenase from T. thermophilus has been cloned, overexpressed, purified and crystallized. Crystals belonging to the orthorhombic space group P212121 produced diffraction data to 1.80 Å.

Online 30 October 2013


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Acta Cryst. (2013). F69, 1252-1255  [ doi:10.1107/S1744309113026146 ]

Cloning, purification and preliminary crystallographic analysis of the complex of Helicobacter pylori [alpha]-carbonic anhydrase with acetazolamide

J. K. Modak, S. A. Revitt-Mills and A. Roujeinikova

Synopsis: [alpha]-Carbonic anhydrase from H. pylori has been purified and crystallized by the hanging-drop vapour-diffusion method. A diffraction data set has been collected to 1.7 Å resolution.

Online 30 October 2013


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Acta Cryst. (2013). F69, 1256-1259  [ doi:10.1107/S1744309113026274 ]

Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Synechocystis sp. PCC 6803

Y. Liu, Y. Zhang, X. Cao and S. Xue

Synopsis: spMCAT from Synechocystis sp. PCC 6803, the first MCAT counterpart from a cyanobacterium, has been successfully cloned, purified and crystallized. A high-quality crystal with good diffraction has been obtained.

Online 30 October 2013


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Acta Cryst. (2013). F69, 1260-1263  [ doi:10.1107/S1744309113026444 ]

Crystallization and preliminary X-ray crystallographic studies of the CIDE-N domain of CIDE-3

S. M. Lee and H. H. Park

Synopsis: The CIDE-N domain of CIDE-3 was purified and crystallized. The crystals were found to belong to space group P32, with unit-cell parameters a = b = 63.35, c = 37.60 Å, [gamma] = 120°. The crystals were obtained at 293 K and diffracted to a resolution of 2.0 Å.

Online 30 October 2013


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Acta Cryst. (2013). F69, 1264-1267  [ doi:10.1107/S174430911302647X ]

Recombinant production, crystallization and preliminary structural characterization of Schistosoma japonicum profilin

N. Vervaet, J. P. Kallio, S. Meier, E. Salmivaara, M. Eberhardt, S. Zhang, X. Sun, Z. Wu, P. Kursula and I. Kursula

Synopsis: Profilin from the helminthic parasite S. japonicum was expressed, purified and crystallized. The protein was found to be folded and to possess the expected secondary-structure content as determined by circular-dichroism spectroscopy.

Online 30 October 2013


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Acta Cryst. (2013). F69, 1268-1271  [ doi:10.1107/S1744309113026559 ]

Preliminary crystallographic analysis of RraB from Escherichia coli

H. Shen, H. Liu, H. Wang, M. Teng and X. Li

Synopsis: The crystallization and preliminary X-ray diffraction analysis of the ribonuclease regulator RraB from E. coli are reported.

Online 30 October 2013


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Acta Cryst. (2013). F69, 1272-1274  [ doi:10.1107/S1744309113026638 ]

Crystallization and preliminary X-ray diffraction analysis of MJ0458, an adenylate kinase from Methanocaldococcus jannaschii

X. Wang, Y. Yuan, M. Teng, L. Niu and Y. Gao

Synopsis: MJ0458 protein, an adenylate kinase from Methanocaldococcus jannaschii which is a rich resource of unique enzymes, was crystallized and a set of X-ray diffraction data to 2.70 Å resolution was collected on the beamline BL-17U of the Shanghai Synchrotron Radiation Facility (SSRF). The crystal belonged to space group P41212 or P43212. The unit-cell parameters were a = b = 76.18, c = 238.70 Å, [alpha] = [beta] = [gamma] = 90°.

Online 30 October 2013


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Acta Cryst. (2013). F69, 1275-1280  [ doi:10.1107/S1744309113026614 ]

Crystallization and preliminary X-ray diffraction analyses of the TIR domains of three TIR-NB-LRR proteins that are involved in disease resistance in Arabidopsis thaliana

L. Wan, X. Zhang, S. J. Williams, T. Ve, M. Bernoux, K. H. Sohn, J. D. G. Jones, P. N. Dodds and B. Kobe

Synopsis: To investigate the molecular basis of immune signalling initiated by the TIR domains of plant disease-resistance proteins, the crystallization and preliminary X-ray diffraction analyses of the TIR domains of three proteins involved in disease resistance in A. thaliana are reported.

Online 30 October 2013


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Acta Cryst. (2013). F69, 1281-1283  [ doi:10.1107/S1744309113026663 ]

Expression, crystallization and preliminary X-ray crystallographic studies of SCP3 coiled-coil domain

E. K. Seo, T. W. Kim and H. H. Park

Synopsis: The C-terminal coiled-coil domain of SCP3, which is part of the lateral element of the synaptonemal complex, was purified and crystallized. The crystals were found to belong to space group C2, with unit-cell parameters a = 121.29, b = 43.08, c = 57.42 Å, [beta] = 100.71°. The crystals were obtained at 293 K and diffracted to a resolution of 3.2 Å.

Online 30 October 2013


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Acta Cryst. (2013). F69, 1284-1287  [ doi:10.1107/S1744309113026754 ]

Crystallization and preliminary X-ray diffraction studies of the reduced form of the terminal oxygenase component of the Rieske nonhaem iron oxygenase system carbazole 1,9a-dioxygenase

J. Matsuzawa, T. Umeda, H. Aikawa, C. Suzuki, Z. Fujimoto, K. Okada, H. Yamane and H. Nojiri

Synopsis: The reduced form of the terminal oxygenase component of carbazole 1,9a-dioxygenase from Janthinobacterium sp. J3 was crystallized. The crystals belonged to space group P65 and diffracted to 1.74 Å resolution.

Online 30 October 2013


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Acta Cryst. (2013). F69, 1288-1290  [ doi:10.1107/S1744309113026729 ]

The base of the ribosomal P stalk from Methanococcus jannaschii: crystallization and preliminary X-ray studies

I. Mitroshin, A. Gabdulkhakov and M. Garber

Synopsis: Crystallization and preliminary X-ray studies of a ternary complex consisting of the ribosomal protein L11, the two-domain N-terminal fragment of the ribosomal protein P0 and a specific fragment of 23S rRNA from the archaeon M. jannaschii are reported.

Online 30 October 2013


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Acta Cryst. (2013). F69, 1291-1294  [ doi:10.1107/S1744309113027164 ]

Cloning, expression, purification, crystallization and preliminary crystallographic analysis of the putative NlpC/P60 endopeptidase, TTHA0266, from Thermus thermophilus HB8

J. E. M. M. Wong and M. Blaise

Synopsis: The crystallization and preliminary crystallographic analysis of the putative NlpC/P60 endopeptidase, TTHA0266 is reported. The crystal belonged to space group P61 and the structure was solved with the single-wavelength anomalous dispersion method.

Online 30 October 2013


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Acta Cryst. (2013). F69, 1295-1298  [ doi:10.1107/S1744309113027784 ]

Overexpression, crystallization and preliminary X-ray crystallographic analysis of release factor eRF1-1 from Arabidopsis thaliana

Y. An, Y. Lou and Y. Xu

Synopsis: Overexpression,crystallization and preliminary X-ray crystallographic analysis of release factor eRF1-1 from Arabidopsis thaliana.

Online 30 October 2013


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Acta Cryst. (2013). F69, 1299-1302  [ doi:10.1107/S1744309113028017 ]

Purification, crystallization and preliminary crystallographic analysis of KatB, a manganese catalase from Anabaena PCC 7120

S. C. Bihani, D. Chakravarty and A. Ballal

Synopsis: KatB, a manganese catalase from Anabaena PCC7120 was crystallized and preliminary diffraction data obtained. Crystal belonged to space group P41212 (or enantiomer) and diffracted to 2.2 Å resolution.

Online 30 October 2013


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Acta Cryst. (2013). F69, 1303-1306  [ doi:10.1107/S1744309113028042 ]

Expression, purification and crystallization of acetyl-CoA hydrolase from Neisseria meningitidis

Y. B. Khandokar, A. Londhe, S. Patil and J. K. Forwood

Synopsis: The crystallization of acetyl-CoA hydrolase from N. meningitidis is reported.

Online 30 October 2013


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Acta Cryst. (2013). F69, 1307-1309  [ doi:10.1107/S174430911302842X ]

Cloning, overexpression, purification and preliminary X-ray analysis of the catalytic domain of the ethylene receptor ETR1 from Arabidopsis thaliana

S. Panneerselvam, H. Kaljunen and J. Mueller-Dieckmann

Synopsis: The catalytic domain of the histidine kinase of the ethylene receptor ETR1 from A. thaliana was expressed and crystallized. The crystals belonged to space group I222 or I212121.

Online 30 October 2013


addenda and errata


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Acta Cryst. (2013). F69, 1310  [ doi:10.1107/S1744309113029114 ]

Crystallization and preliminary X-ray study of a thermostable alanine racemase from Thermoanaerobacter tengcongensis MB4. Corrigendum

H. Dong, S. Xu, X. Lu, G. He, R. Zhao, S. Chen, S. Fu and J. Ju

Synopsis: A correction is made to the article by Dong et al. (2013). Acta Cryst. F69, 660-662.

Online 31 October 2013


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Acta Cryst. (2013). F69, 1311  [ doi:10.1107/S1744309113029138 ]

Crystallization and preliminary X-ray analysis of an alanine dehydrogenase from Bacillus megaterium WSH-002. Corrigendum

X. Lu, Q. Yi, G. Zhang, X. Zhu, H. Zhou and H. Dong

Synopsis: A correction is made to the article by Lu et al. (2013). Acta Cryst. F69, 934-936.

Online 31 October 2013


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Acta Cryst. (2013). F69, 1312  [ doi:10.1107/S174430911302914X ]

Production, crystallization and X-ray diffraction analysis of two nanobodies against the Duffy binding-like (DBL) domain DBL6[epsilon]-FCR3 of the Plasmodium falciparum VAR2CSA protein. Corrigendum

A. Vuchelen, E. Pardon, J. Steyaert, B. Gamain, R. Loris, N. A. J. van Nuland and S. Ramboarina

Synopsis: A correction to the article by Vuchelen et al. (2013). Acta Cryst. F69, 270-274.

Online 31 October 2013


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Acta Cryst. (2013). F69, 1313  [ doi:10.1107/S1744309113029126 ]

Production and crystallization of [alpha]-phosphoglucomutase from Lactococcus lactis. Corrigendum

P. Nogly, R. Castro, M. de Rosa, A. R. Neves, H. Santos and M. Archer

Synopsis: A correction is made to the article by Nogly et al. (2012). Acta Cryst. F68, 1113-1115.

Online 31 October 2013


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Acta Cryst. (2013). F69, 1314  [ doi:10.1107/S1744309113029321 ]

Crystallization and preliminary X-ray study of the deaminase AmnE from Pseudomonas sp. AP-3. Corrigendum

D. Yu, Y. Jiang, J. Hou, S. Chen, G. Zhang, X. Liu, H. Dong and B. Yu

Synopsis: A correction is made to the article by Yu et al. (2013). Acta Cryst. F69, 812-814.

Online 31 October 2013


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