Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 69, Part 12 (December 2013)



[Issue Author Index][Volume Author Index]
[Cover illustration] Cover illustration: Structure of the uracil complex of Vaccinia virus uracil DNA glycosylase (Schormann et al., p. 1328).

editorial


 

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Acta Cryst. (2013). F69, 1315  [ doi:10.1107/S1744309113031990 ]

Big changes are ahead - a new format for crystallization communications in Acta Cryst. F

M. S. Weiss and H. Einspahr

Synopsis: The Editors of Acta F look forward to 2014.

Online 30 November 2013


structural communications


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Acta Cryst. (2013). F69, 1316-1321  [ doi:10.1107/S1744309113029199 ]

Coiled-coil dimerization of the LOV2 domain of the blue-light photoreceptor phototropin 1 from Arabidopsis thaliana

A. S. Halavaty and K. Moffat

Synopsis: The X-ray structure of the light, oxygen or voltage domain 2 of phototropin 1 from A. thaliana (AtLOV2) in its dark-adapted state has been determined. The N-terminal flanking A'[alpha] helix of AtLOV2 plays a role in its dimerization.

PDB reference: 4hhd

Online 28 November 2013


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Acta Cryst. (2013). F69, 1322-1327  [ doi:10.1107/S174430911302962X ]

Structure of the secretion domain of HxuA from Haemophilus influenzae

S. Baelen, F. Dewitte, B. Clantin and V. Villeret

Synopsis: The structure of the secretion domain of HxuA from H. influenzae has been determined to 1.5 Å resolution.

PDB reference: 4i84

Online 28 November 2013


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Acta Cryst. (2013). F69, 1328-1334  [ doi:10.1107/S1744309113030613 ]

Structure of the uracil complex of Vaccinia virus uracil DNA glycosylase

N. Schormann, S. Banerjee, R. Ricciardi and D. Chattopadhyay

Synopsis: The crystal structure of the uracil complex of Vaccinia virus uracil DNA glycosylase (D4) has been determined at 2.03 Å resolution.

PDB reference: 4lzb

Online 28 November 2013


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Acta Cryst. (2013). F69, 1335-1338  [ doi:10.1107/S1744309113030005 ]

Structure of Acidothermus cellulolyticus family 74 glycoside hydrolase at 1.82 Å resolution

M. Alahuhta, W. S. Adney, M. E. Himmel and V. V. Lunin

Synopsis: The 1.82 Å resolution X-ray structure of a thermally tolerant glycoside hydrolase family 74 enzyme from A. cellulolyticus is reported.

PDB reference: 4lgn

Online 28 November 2013


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Acta Cryst. (2013). F69, 1339-1343  [ doi:10.1107/S1744309113031448 ]

Two high-resolution structures of the human E3 ubiquitin ligase Siah1

V. Rimsa, T. C. Eadsforth and W. N. Hunter

Synopsis: Two structures of human Siah1 at 1.95 and 1.58 Å resolution provide more complete models for this protein and identify conformational variability in the subdomain organization.

PDB references: 4c9z and 4ca1

Online 28 November 2013


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Acta Cryst. (2013). F69, 1344-1348  [ doi:10.1107/S1744309113030534 ]

Structure of the [beta]-form of human MK2 in complex with the non-selective kinase inhibitor TEI-L03090

A. Fujino, K. Fukushima, T. Kubota, Y. Matsumoto and M. Takimoto-Kamimura

Synopsis: The glycine-rich loop of MK2 (MAPKAP-K2) bound to the non-selective inhibitor TEI-L03090 adopts the [beta]-form, which is commonly found in other kinase complexes; however, it differs from the [alpha]-form which is adopted when MK2 is bound to the MK2-selective inhibitor TEI-I01800. These results show how conformational change of MK2 influences its selectivity.

PDB reference: 3wi6

Online 28 November 2013


crystallization communications


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Acta Cryst. (2013). F69, 1349-1353  [ doi:10.1107/S1744309113028054 ]

Crystallization and preliminary X-ray crystallographic analysis of the curli transporter CsgG

P. Goyal, N. Van Gerven, W. Jonckheere and H. Remaut

Synopsis: Structural insights into the type-VIII protein secretion system are described.

Online 28 November 2013


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Acta Cryst. (2013). F69, 1354-1356  [ doi:10.1107/S1744309113027000 ]

Purification, crystallization and preliminary X-ray studies of MbtN (Rv1346) from Mycobacterium tuberculosis

A.-F. Chai, J. M. Johnston, R. D. Bunker, E. M. M. Bulloch, G. L. Evans, J. S. Lott and E. N. Baker

Synopsis: MbtN (Rv1346) from M. tuberculosis was cloned, overexpressed, purified and crystallized. The crystals diffracted to 2.3 Å resolution.

Online 28 November 2013


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Acta Cryst. (2013). F69, 1357-1359  [ doi:10.1107/S1744309113028704 ]

Crystallization and preliminary X-ray crystallographic analysis of human peptidylarginine deiminase type I

M. Unno, S. Kinjo, K. Kizawa and H. Takahara

Synopsis: Human peptidylarginine deiminase type I was crystallized using polyethylene glycol as a precipitant. The crystals belonged to space group P61, with unit-cell parameters a = b = 90.3, c = 372.3 Å, and contained two molecules in the asymmetric unit.

Online 28 November 2013


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Acta Cryst. (2013). F69, 1360-1362  [ doi:10.1107/S1744309113028935 ]

Crystallization and preliminary X-ray diffraction analysis of an active-site mutant of `loopless' family GH19 chitinase from Bryum coronatum in a complex with chitotetraose

T. Ohnuma, N. Umemoto, T. Taira, T. Fukamizo and T. Numata

Synopsis: A selenomethionine-labelled active-site mutant of `loopless' family GH19 chitinase from B. coronatum has been co-crystallized with chitotetraose. X-ray diffraction data were collected to 1.58 Å resolution using a synchrotron-radiation source.

Online 28 November 2013


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Acta Cryst. (2013). F69, 1363-1367  [ doi:10.1107/S1744309113028959 ]

Crystallization of the C-terminal domain of the bacteriophage T5 L-shaped fibre

C. Garcia-Doval, D. Luque, J. R. Castón, P. Boulanger and M. J. van Raaij

Synopsis: The trimeric L-shaped fibre protein from bacteriophage T5 has been analysed by electron microscopy and a C-terminal domain has been crystallized. Diffraction data have been obtained for two native crystal forms and one selenomethionine derivative.

Online 28 November 2013


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Acta Cryst. (2013). F69, 1368-1370  [ doi:10.1107/S1744309113029072 ]

Two-dimensional crystallization of intact F-ATP synthase isolated from bovine heart mitochondria

S. Maeda, K. Shinzawa-Itoh, K. Mieda, M. Yamamoto, Y. Nakashima, Y. Ogasawara, C. Jiko, K. Tani, A. Miyazawa, C. Gerle and S. Yoshikawa

Synopsis: Two-dimensional crystallization conditions for intact bovine F-ATP synthase have been established.

Online 29 November 2013


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Acta Cryst. (2013). F69, 1371-1373  [ doi:10.1107/S1744309113029230 ]

Crystallization and preliminary crystallographic analysis of the chimeric protein LKB1-14-3-3[zeta]

S. Ding, R. Zhou and Y. Zhu

Synopsis: The chimeric protein LKB1-14-3-3[zeta] was purified and crystallized. The X-ray diffraction were collected to a resolution of 2.9-Å.

Online 29 November 2013


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Acta Cryst. (2013). F69, 1374-1379  [ doi:10.1107/S1744309113029308 ]

Crystallization of the C-terminal domain of the fibre protein from snake adenovirus 1, an atadenovirus

A. K. Singh, R. Menéndez-Conejero, C. San Martín and M. J. van Raaij

Synopsis: A C-terminal domain of the fibre protein from snake adenovirus 1, an atadenovirus, has been crystallized. Diffraction data have been obtained to high resolution for several native crystal forms and a selenomethionine derivative.

Online 29 November 2013


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Acta Cryst. (2013). F69, 1380-1383  [ doi:10.1107/S1744309113029734 ]

Crystallization and preliminary crystallographic analysis of a surface antigen glycoprotein, SAG19, from Eimeria tenella

N. Z. Ramly, S. N. Rouzheinikov, S. E. Sedelnikova, P. J. Baker, Y.-P. Chow, K.-L. Wan, S. Nathan and D. W. Rice

Synopsis: Recombinant SAG19 from E. tenella has been overexpressed, purified and crystallized in a form suitable for X-ray analysis.

Online 29 November 2013


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Acta Cryst. (2013). F69, 1384-1388  [ doi:10.1107/S174430911302976X ]

Crystallization and preliminary X-ray analysis of the ATPase domain of the [sigma]54-dependent transcription activator NtrC1 from Aquifex aeolicus bound to the ATP analog ADP-BeFx

T. A. Sysoeva, N. Yennawar, M. Allaire and B. T. Nixon

Synopsis: This study reports the crystallization of a new nucleotide state of the ATPase domain of a bacterial transcription activator NtrC1 from the hyperthermophilic bacterium Aquifex aeolicus. Wild-type NtrC1 ATPase domain was crystallized in the presence of the ATP analog ADP-BeFx-Mg and the crystals diffracted anisotropically to at best 3.2, 5.2 and 3.2 Å resolution in the a*, b* and c* directions, respectively.

Online 29 November 2013


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Acta Cryst. (2013). F69, 1389-1392  [ doi:10.1107/S1744309113029990 ]

Crystallization and sulfur SAD phasing of AggA, the major subunit of aggregative adherence fimbriae type I from the Escherichia coli strain that caused an outbreak of haemolytic-uraemic syndrome in Germany

N. Pakharukova, M. Tuittila and A. Zavialov

Synopsis: Donor-strand complemented AggA subunit of aggregative adherence fimbriae type I from Shiga toxin-producing E. coli O104:H4 was crystallized. Diffraction data were collected to 1.55 Å resolution. Initial phases were derived from a sulfur single anomalous diffraction experiment.

Online 29 November 2013


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Acta Cryst. (2013). F69, 1393-1396  [ doi:10.1107/S1744309113030133 ]

Crystallization of the CHAP domain of the endolysin from Staphylococcus aureus bacteriophage K

M. Sanz-Gaitero, R. Keary, C. Garcia-Doval, A. Coffey and M. J. van Raaij

Synopsis: The N-terminal cysteine, histidine-dependent amidohydrolase (peptidase) domain of the S. aureus bacteriophage K endolysin LysK has been crystallized. Native and derivative data to a resolution of 1.7-1.8 Å have been obtained, which allowed structure solution.

Online 29 November 2013


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Acta Cryst. (2013). F69, 1397-1400  [ doi:10.1107/S1744309113030480 ]

Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa

T. Furuyama, Y. Ishii, N. Ohya, K. Tateda, N. D. Hanson and A. Shimizu-Ibuka

Synopsis: The class B carbapenemase IMP-18 from P. aeruginosa was overexpressed in E. coli, purified and crystallized. The crystals belonged to space group P41212 and diffracted to 2 Å resolution.

Online 29 November 2013


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Acta Cryst. (2013). F69, 1401-1404  [ doi:10.1107/S1744309113030649 ]

Preliminary crystallographic studies of BRCA1 BRCT-ABRAXAS complex

D. C. Badgujar, U. Sawant, Vikrant, L. Yadav, M. V. Hosur and A. K. Varma

Synopsis: The crystallization and preliminary X-ray diffraction analysis of BRCA1 BRCT with ABRAXAS phosphopeptide are reported. Binding affinity using ITC has been compared.

Online 29 November 2013


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Acta Cryst. (2013). F69, 1405-1410  [ doi:10.1107/S1744309113030650 ]

Liquid-liquid diffusion crystallization improves the X-ray diffraction of EndoS, an endo-[beta]-N-acetyl­glucosaminidase from Streptococcus pyogenes with activity on human IgG

B. Trastoy, J. V. Lomino, L.-X. Wang and E. J. Sundberg

Synopsis: A comparative study of vapor diffusion versus liquid-liquid diffusion methods used for the crystallization of EndoS is reported. X-ray diffraction data to 2.6 and 1.9 Å resolution were collected for wild-type endoglycosidase and glycosynthase constructs of EndoS, respectively.

Online 29 November 2013


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Acta Cryst. (2013). F69, 1411-1414  [ doi:10.1107/S1744309113030819 ]

Crystallization and preliminary X-ray crystallographic analysis of the inhibitory domain of the tomato mosaic virus resistance protein Tm-1

M. Kato, Y. Kezuka, C. Kobayashi, K. Ishibashi, T. Nonaka, M. Ishikawa and E. Katoh

Synopsis: A crystal of Tm-1, which is an inhibitor protein of Tomato mosaic virus RNA replication, was obtained using the hanging-drop vapour-diffusion method at 293 K and diffracted X-rays to 2.7 Å resolution. It belonged to space group P1, with unit-cell parameters a = 77.97, b = 105.28, c = 110.62 Å, [alpha] = 94.6, [beta] = 109.3, [gamma] = 108.0°.

Online 29 November 2013


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Acta Cryst. (2013). F69, 1415-1417  [ doi:10.1107/S1744309113030923 ]

Crystallization and preliminary X-ray diffraction studies of the (R)-selective amine transaminase from Aspergillus fumigatus

M. Thomsen, L. Skalden, G. J. Palm, M. Höhne, U. T. Bornscheuer and W. Hinrichs

Synopsis: The (R)-selective amine transaminase from A. fumigatus was purified and crystallized. Diffraction data to 1.84 Å resolution for S-SAD phasing as well as high-resolution data to 1.27 Å were collected from a single crystal belonging to space group C2221.

Online 29 November 2013


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Acta Cryst. (2013). F69, 1418-1421  [ doi:10.1107/S1744309113030972 ]

Expression, purification, crystallization and preliminary X-ray analysis of the receiver domain of Staphylococcus aureus LytR protein

A. Shala, K. H. Patel, D. Golemi-Kotra and G. F. Audette

Synopsis: The expression, purification, crystallization and preliminary X-ray diffraction analysis of the N-terminal receiver domain of LytR, LytRN, from S. aureus is reported.

Online 29 November 2013


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Acta Cryst. (2013). F69, 1422-1424  [ doi:10.1107/S1744309113031138 ]

Cloning, expression, purification, crystallization and preliminary X-ray studies of argininosuccinate lyase (Rv1659) from Mycobacterium tuberculosis

A. Paul, A. Mishra, A. Surolia and M. Vijayan

Synopsis: Argininosuccinate lyase from M. tuberculosis has been cloned, purified and crystallized, and preliminary X-ray studies on the crystals have been carried out.

Online 29 November 2013


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Acta Cryst. (2013). F69, 1425-1430  [ doi:10.1107/S1744309113031114 ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the aspartate transcarbamoylase domain of human CAD

A. Ruiz-Ramos, N. Lallous, A. Grande-García and S. Ramón-Maiques

Synopsis: The recombinant aspartate transcarbamoylase domain of human CAD was expressed in E. coli, purified and crystallized in the presence and absence of the inhibitor PALA. X-ray diffraction data sets were collected for both crystal forms at 2.1 Å resolution.

Online 29 November 2013


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Acta Cryst. (2013). F69, 1431-1435  [ doi:10.1107/S1744309113023592 ]

Purification, crystallization and preliminary crystallographic analysis of the ligand-binding regions of the PctA and PctB chemoreceptors from Pseudomonas aeruginosa in complex with amino acids

M. Rico-Jiménez, F. Muñoz-Martínez, T. Krell, J. A. Gavira and E. Pineda-Molina

Synopsis: To determine the molecular basis underlying the ligand specificity of the ligand-binding regions of Pseudomonas chemotactic transducer A and B, complexes with amino acids have been purified and crystals have been produced.

Online 29 November 2013


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Acta Cryst. (2013). F69, 1436-1439  [ doi:10.1107/S174430911303145X ]

Crystallization and preliminary X-ray diffraction analysis of D53H mutant Escherichia coli cAMP receptor protein

J. Huang, T. Wu, Z. Guo, T. Lou, S. Yu, W. Gong and C. Ji

Synopsis: The Escherichia coli cyclic AMP receptor protein (CRP) is a prokaryotic global transcription activator protein that controls the expression of many different genes. cAMP-free D53H CRP crystals were obtained and diffracted at a resolution of 2.9 Å. Based on the systematic absences of the crystals, the likely space group is P212121 with the unit-cell parameters a = 76.66, b = 152.14, and c = 176.11 Å. The asymmetric unit was confirmed to contain four protein dimers with a Matthews coefficient of 2.71 Å3 Da-1 and a solvent content of 54.68%.

Online 29 November 2013


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Acta Cryst. (2013). F69, 1440-1442  [ doi:10.1107/S1744309113025050 ]

Overexpression, crystallization and preliminary X-ray crystallographic analysis of glucuronoxylan xylanohydrolase (Xyn30A) from Clostridium thermocellum

A. K. Verma, A. Goyal, F. Freire, P. Bule, I. Venditto, J. L. A. Brás, H. Santos, V. Cardoso, C. Bonifácio, A. Thompson, M. J. Romão, J. A. M. Prates, L. M. A. Ferreira, C. M. G. A. Fontes and S. Najmudin

Synopsis: The glycoside hydrolase family 30 module from the cellulosomal C. thermocellum XynGH30 was purified and crystallized and data were collected to 1.4 Å resolution.

Online 30 November 2013


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