Acta Crystallographica Section F

Structural Biology Communications

Volume 70, Part 4 (April 2014)



[Issue Author Index][Volume Author Index]
[Cover illustration] Cover illustration: The trimeric structure of divalent cation tolerance protein CutA1 from Thermus thermophilus (Bagautdinov, p. 404).

IYCr crystallization series


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Acta Cryst. (2014). F70, 384-403  [ doi:10.1107/S2053230X14004816 ]

Mechanisms, kinetics, impurities and defects: consequences in macromolecular crystallization

A. McPherson and Y. G. Kuznetsov

Synopsis: New imaging techniques, particularly AFM, permitted the elucidation of the mechanisms for protein and virus crystal growth. They have also allowed direct visualization of crystal defect structure and the consequences of impurity incorporation.

Online 29 March 2014


structural communications


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Acta Cryst. (2014). F70, 404-413  [ doi:10.1107/S2053230X14003422 ]

The structures of the CutA1 proteins from Thermus thermophilus and Pyrococcus horikoshii: characterization of metal-binding sites and metal-induced assembly

B. Bagautdinov

Synopsis: The crystal structures of CutA1 from T. thermophilus HB8 with and without bound Na+ and CutA1 from P. horikoshii OT3 in complex with Na+ have been determined in order to understand metal binding and metal-driven assembly features of the proteins.

PDB references: 1nza, 1v6h and 4nyo

Online 25 March 2014


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Acta Cryst. (2014). F70, 414-417  [ doi:10.1107/S2053230X14003793 ]

1.55 Å resolution X-ray crystal structure of Rv3902c from Mycobacterium tuberculosis

B. G. Reddy, D. B. Moates, H.-B. Kim, T. J. Green, C.-Y. Kim, T. C. Terwilliger and L. J. DeLucas

Synopsis: The 1.55 Å resolution X-ray crystal structure of Rv3902c from M. tuberculosis reveals a novel fold.

PDB reference: 4o6g

Online 25 March 2014


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Acta Cryst. (2014). F70, 418-423  [ doi:10.1107/S2053230X14003884 ]

1.15 Å resolution structure of the proteasome-assembly chaperone Nas2 PDZ domain

C. R. Singh, S. Lovell, N. Mehzabeen, W. Q. Chowdhury, E. S. Geanes, K. P. Battaile and J. Roelofs

Synopsis: The proteasome-assembly chaperone Nas2 binds to the proteasome subunit Rpt5 using its PDZ domain. The structure of the Nas2 PDZ domain has been determined.

PDB reference: 4o06

Online 25 March 2014


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Acta Cryst. (2014). F70, 424-432  [ doi:10.1107/S2053230X14003926 ]

Structure of 4-pyridoxolactonase from Mesorhizobium loti

J. Kobayashi, Y. Yoshikane, T. Yagi, S. Baba, K. Mizutani, N. Takahashi and B. Mikami

Synopsis: The structure of 4-pyridoxolactonase from M. loti was determined.

PDB references: 3aj3, 3aj0 and 4keq

Online 25 March 2014


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Acta Cryst. (2014). F70, 433-437  [ doi:10.1107/S2053230X14004166 ]

Comparison of four different crystal forms of the Mycobacterium tuberculosis ESX-1 secreted protein regulator EspR

S. P. Gangwar, S. R. Meena and A. K. Saxena

Synopsis: Structural comparison of four crystal forms of Mycobacterium tuberculosis EspR protein.

PDB reference: 4ndw

Online 25 March 2014


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Acta Cryst. (2014). F70, 438-443  [ doi:10.1107/S2053230X14004774 ]

Structure of the kinase domain of Gilgamesh from Drosophila melanogaster

N. Han, C. Chen, Z. Shi and D. Cheng

Synopsis: The structure of the kinase domain of Gilgamesh from Drosophila melanogaster was determined at 2.85 Å resolution.

PDB reference: 4nt4

Online 25 March 2014


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Acta Cryst. (2014). F70, 444-449  [ doi:10.1107/S2053230X14004695 ]

Structure of human nucleosome containing the testis-specific histone variant TSH2B

T. Urahama, N. Horikoshi, A. Osakabe, H. Tachiwana and H. Kurumizaka

Synopsis: The crystal structure of human nucleosome containing the testis-specific TSH2B variant has been determined. The TSH2B Ser85 residue does not interact with H4 in the nucleosome, and induces a local structural difference between TSH2B and H2B in nucleosomes.

PDB reference: 3wkj

Online 25 March 2014


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Acta Cryst. (2014). F70, 450-456  [ doi:10.1107/S2053230X14004051 ]

Structural characterization of a novel autonomous cohesin from Ruminococcus flavefaciens

M. Voronov-Goldman, M. Levy-Assaraf, O. Yaniv, G. Wisserman, S. Jindou, I. Borovok, E. A. Bayer, R. Lamed, L. J. W. Shimon and F. Frolow

Synopsis: The crystal structure of a standalone cohesin protein from the rumen bacterium R. flavefaciens has been determined at 2.44 Å resolution, revealing an elaborate set of secondary-structural elements.

PDB reference: 4n2o

Online 25 March 2014


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Acta Cryst. (2014). F70, 457-460  [ doi:10.1107/S2053230X14003811 ]

Structure of the Reston ebolavirus VP30 C-terminal domain

M. C. Clifton, R. N. Kirchdoerfer, K. Atkins, J. Abendroth, A. Raymond, R. Grice, S. Barnes, S. Moen, D. Lorimer, T. E. Edwards, P. J. Myler and E. O. Saphire

Synopsis: The crystal structure of the Reston ebolavirus VP30 C-terminal domain shows a rotated interface in comparison to the previous structure of the Zaire ebolavirus VP30 C-terminal domain.

PDB reference: 3v7o

Online 25 March 2014


crystallization communications


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Acta Cryst. (2014). F70, 461-463  [ doi:10.1107/S2053230X13032755 ]

Crystallization and preliminary X-ray diffraction analysis of domain-chimeric L-(2S,3S)-butanediol dehydrogenase

T. Shimegi, T. Ooyama, T. Ohtsuki, G. Kurisu, M. Kusunoki and S. Ui

Synopsis: Crystallization of a domain-chimeric L-2,3-butanediol dehydrogenase (L-BDH) designed to possess both the S-configuration specificity of L-BDH and the stability of meso-BDH.

Online 25 March 2014


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Acta Cryst. (2014). F70, 464-466  [ doi:10.1107/S2053230X14001940 ]

Crystallization and preliminary X-ray crystallographic analysis of [alpha]-glucosidase HaG from Halomonas sp. strain H11

X. Shen, W. Saburi, Z.-Q. Gai, K. Komoda, J. Yu, T. Ojima-Kato, Y. Kido, H. Matsui, H. Mori and M. Yao

Synopsis: The recombinant [alpha]-glucosidase HaG from Halomonas sp. Strain H11 was overexpressed, purified and crystallized to 2.15 Å, and its structure has been determined by molecular replacement.

Online 25 March 2014


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Acta Cryst. (2014). F70, 467-469  [ doi:10.1107/S2053230X14004014 ]

Expression, purification, crystallization and preliminary X-ray diffraction analysis of a mammalian type 10 adenylyl cyclase

S. Kleinboelting, J. van den Heuvel, C. Kambach, M. Weyand, M. Leipelt and C. Steegborn

Synopsis: Among the mammalian adenylyl cyclases, the soluble type 10 cyclase is unique in its activation by bicarbonate and calcium and its lack of a transmembrane region. Here, the recombinant production and crystallization of the catalytic core of the human type 10 adenylyl cyclase are described.

Online 25 March 2014


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Acta Cryst. (2014). F70, 470-472  [ doi:10.1107/S2053230X14004087 ]

Crystallization and preliminary neutron diffraction experiment of human farnesyl pyrophosphate synthase complexed with risedronate

T. Yokoyama, A. Ostermann, M. Mizuguchi, N. Niimura, T. E. Schrader and I. Tanaka

Synopsis: Neutron diffraction studies of human farnesyl pyrophosphate synthase are reported.

Online 25 March 2014


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Acta Cryst. (2014). F70, 473-475  [ doi:10.1107/S2053230X14004142 ]

Crystallization and preliminary X-ray analysis of a novel type of lipolytic hydrolase from Bacillus licheniformis

H. Ju, R. Pandian, K. Kim, K. K. Kim and T. D. Kim

Synopsis: The hydrolase BL28 from B. licheniformis was crystallized in the tetragonal space group P41212 and diffraction data were collected to 1.67 Å resolution.

Online 25 March 2014


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Acta Cryst. (2014). F70, 476-481  [ doi:10.1107/S2053230X14004129 ]

Preliminary crystallographic analysis of a double mutant of the acetyl xylo-oligosaccharide esterase Axe2 in its dimeric form

S. Lansky, O. Alalouf, R. Salama, H. Dvir, Y. Shoham and G. Shoham

Synopsis: A rational double mutation in the serine acetyl xylo-oligosaccharide esterase from G. stearothermophilus T6 (Axe2) converts the enzyme from an octameric form to a dimeric form. The dimeric double mutant (Axe2-Y184F-W190P) has been crystallized in the orthorhombic space group P212121 and a complete X-ray diffraction data set has been measured for these crystals at 2.3 Å resolution for use in a full three-dimensional structural analysis of this form of the protein.

Online 25 March 2014


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Acta Cryst. (2014). F70, 482-484  [ doi:10.1107/S2053230X14004130 ]

Purification, crystallization and X-ray crystallographic studies on a putative methyltransferase, YtqB, from Bacillus subtilis

S. C. Park, W. S. Song, J. Wi and S. Yoon

Synopsis: A putative methyltransferase, YtqB, from B. subtilis was overexpressed, purified and crystallized. X-ray diffraction data were collected from a YtqB crystal to 1.68 Å resolution.

Online 25 March 2014


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Acta Cryst. (2014). F70, 485-488  [ doi:10.1107/S2053230X14004348 ]

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of (S)-3-hydroxy­butyryl-CoA dehydrogenase from Clostridium butyricum

E.-J. Kim and K.-J. Kim

Synopsis: 3-Hydroxybutyryl-CoA dehydrogenase from C. butyricum, an enzyme involved in n-butanol production, has been crystallized and X-ray diffraction data from the crystal have been collected and analyzed.

Online 25 March 2014


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Acta Cryst. (2014). F70, 489-492  [ doi:10.1107/S2053230X14004543 ]

Cloning, crystallization and preliminary X-ray diffraction analysis of an intact DNA methyltransferase of a type I restriction-modification enzyme from Vibrio vulnificus

L. Huynh Thi Yen, S.-Y. Park and J.-S. Kim

Synopsis: The single-chain methyltransferase of a type I restriction-modification system was expressed and crystallized, and diffraction data were collected to a resolution of 2.31 Å.

Online 25 March 2014


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Acta Cryst. (2014). F70, 493-496  [ doi:10.1107/S2053230X14004798 ]

Overexpression, crystallization and preliminary X-ray crystallographic analysis of the ectoine hydroxylase from Sphingopyxis alaskensis

A. Hoeppner, N. Widderich, E. Bremer and S. H. J. Smits

Synopsis: The ectoine hydroxylase from S. alaskensis was crystallized, yielding crystals diffracting to 2.1 Å resolution. The addition of iron prior to crystallization led to a different type of crystal, which may represent the iron-bound form of the enzyme.

Online 25 March 2014


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Acta Cryst. (2014). F70, 497-500  [ doi:10.1107/S2053230X14004865 ]

Purification, crystallization and preliminary X-ray diffraction analysis of ArsH from Synechocystis sp. strain PCC 6803

X. Zhang, X.-M. Xue, Y. Yan and J. Ye

Synopsis: ArsH from Synechocystis sp. strain PCC 6803 was overproduced, purified and crystallized. Diffraction data were processed to a resolution of 1.6 Å. The crystals belonged to the tetragonal space group I4122, with unit-cell parameters a = b = 127.94, c = 65.86 Å.

Online 25 March 2014


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Acta Cryst. (2014). F70, 501-504  [ doi:10.1107/S2053230X14005068 ]

Crystallization and preliminary X-ray crystallographic analysis of importin-[alpha] from Neurospora crassa

N. E. Bernardes, A. A. S. Takeda, F. Z. Freitas, M. C. Bertolini and M. R. M. Fontes

Synopsis: The nuclear import receptor importin-[alpha] from the filamentous fungus N. crassa has been crystallized in the presence of a classical NLS peptide. An X-ray diffraction data set was collected and a molecular-replacement solution was obtained.

Online 25 March 2014


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Acta Cryst. (2014). F70, 505-508  [ doi:10.1107/S2053230X14005081 ]

Expression, crystallization and preliminary X-ray crystallographic analysis of D-alanine-D-alanine ligase from OXA-23-producing Acinetobacter baumannii K0420859

K. H. Huynh, H.-T. Tran, T.-V. Pham, H.-P.-T. Ngo, S.-S. Cha, K. M. Chung, S. H. Lee and L.-W. Kang

Synopsis: OXA-23-producing A. baumannii K0420859 can resist the action of carbapenem, and causes bacteraemia, pneumonia, other respiratory-tract and urinary-tract infections in humans. As a novel target for an antibacterial drug against A. baumannii OXA-23, D-alanine-D-alanine ligase from A. baumannii was purified and crystallized, and a primary X-ray crystallographic analysis was performed.

Online 25 March 2014


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Acta Cryst. (2014). F70, 509-512  [ doi:10.1107/S2053230X14005093 ]

Preliminary crystallographic analysis of the ankyrin-repeat domain of Arabidopsis thaliana AKT1: identification of the domain boundaries for protein crystallization

A. Chaves-Sanjuán, M. J. Sánchez-Barrena, J. M. González-Rubio and A. Albert

Synopsis: The construct optimization, purification, crystallization and preliminary crystallographic analysis of the ankyrin-repeat domain of Arabidopsis thaliana potassium transporter AKT1 is reported.

Online 25 March 2014


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Acta Cryst. (2014). F70, 513-516  [ doi:10.1107/S2053230X1400510X ]

Crystallization and preliminary X-ray crystallographic studies of transglutaminase 2 in complex with Ca2+

T.-H. Jang and H. H. Park

Synopsis: Human transglutaminase 2 (TG2) in complex with Ca2+ was crystallized and the crystals were found to belong to the orthorhombic space group C2221, with unit-cell parameters a = 133.08, b = 216.30, c = 166.26 Å. The crystals were obtained at 20°C and diffracted to a resolution of 3.4 Å.

Online 25 March 2014


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Acta Cryst. (2014). F70, 517-521  [ doi:10.1107/S2053230X14005196 ]

Recombinant production, purification and crystallization of the Toxoplasma gondii coronin WD40 domain

J. P. Kallio and I. Kursula

Synopsis: The T. gondii coronin WD40 domain with the conserved region was recombinantly produced, purified and crystallized. A diffraction data set was collected to 1.65 Å resolution.

Online 25 March 2014


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Acta Cryst. (2014). F70, 522-525  [ doi:10.1107/S2053230X14005548 ]

Crystallization and preliminary crystallographic analysis of manganese lipoxygenase

A. Wennman, E. H. Oliw and S. Karkehabadi

Synopsis: Manganese lipoxygenase of Geaumannomyces graminis was crystallized and X-ray diffraction data were collected to a resolution of 2.6 Å.

Online 25 March 2014


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Acta Cryst. (2014). F70, 526-529  [ doi:10.1107/S2053230X14005342 ]

Successful data recovery from oscillation photographs containing strong polycrystalline diffraction rings from an unknown small-molecule contaminant: preliminary structure solution of Salmonella typhimurium pyridoxal kinase (PdxK)

G. Deka, J. N. Kalyani, J. F. Benazir, H. S. Savithri and M. R. N. Murthy

Synopsis: Pyridoxal kinase (PdxK) from S. typhimurium was cloned, purified and crystallized. Successful structure solution was accomplished in spite of strong polycrystalline noise in the oscillation images.

Online 25 March 2014


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Acta Cryst. (2014). F70, 530-534  [ doi:10.1107/S2053230X14005408 ]

Frq2 from Drosophila melanogaster: cloning, expression, purification, crystallization and preliminary X-ray analysis

S. Baños-Mateos, A. Chaves-Sanjuán, A. Mansilla, A. Ferrús and M. J. Sánchez-Barrena

Synopsis: The calcium-binding protein Frq2 from D. melanogaster has been crystallized and X-ray diffraction data from two different crystal forms have been collected.

Online 25 March 2014


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Acta Cryst. (2014). F70, 535-537  [ doi:10.1107/S2053230X14005470 ]

Crystallization and preliminary X-ray crystallographic analysis of PBPD2 from Listeria monocytogenes

H. J. Cha, J.-H. Jeong and Y.-G. Kim

Synopsis: The crystallization of PBPD2 from L. monocytogenes is described.

Online 25 March 2014


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Acta Cryst. (2014). F70, 538-542  [ doi:10.1107/S2053230X13020414 ]

Notes for authors

Online 31 March 2014


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