Crystallization screening: the influence of history on current practice

The rich history of crystallization and how that history influences current practices is described. The tremendous impact of crystallization screens on the field is discussed.

Monomer structure of a hyperthermophilic β-glucosidase mutant forming a dodecameric structure in the crystal form

A protein-engineering study revealed that the C-terminal domain of a thermostable β-glucosidase contributes to its polymeric state.

Structure of d(CCCCGGTACCGGGG)₂ at 1.65 Å resolution

The crystal structure of the tetradecanucleotide d(CCCCGGTACCGGGG)₂ as an A-DNA duplex and its solvent interactions are described.

Structure of Rhodococcus equi virulence-associated protein B (VapB) reveals an eight-stranded antiparallel β-barrel consisting of two Greek-key motifs

The structure of VapB, a member of the Vap protein family that is involved in virulence of the bacterial pathogen R. equi, was determined by SAD phasing and reveals an eight-stranded antiparallel β-barrel similar to avidin, suggestive of a binding function. Made up of two Greek-key motifs, the topology of VapB is unusual or even unique.

Recombinant production, crystallization and X-ray crystallographic structure determination of peptidyl-tRNA hydrolase from Salmonella typhimurium

S. typhimurium peptidyl-tRNA hydrolase was cloned, expressed, purified and crystallized. The crystals obtained diffracted to 1.6 Å resolution.

A new crystal form of a hyperthermophilic endocellulase

The hyperthermostable endocellulase from P. furiosus was crystallized at pH 5.5. The new crystal form has symmetry consistent with space group C2 and exhibits a structure different from that of the protein crystallized at pH 9.0.

Crystallization of dienelactone hydrolase in two space groups: structural changes caused by crystal packing

Crystallization of dienelactone hydrolase in an alternative space group results in more crystal contacts between neighbouring molecules and changes in the side-chain orientations at the new crystallographic interfaces.

Structure of D-tagatose 3-epimerase-like protein from Methanocaldococcus jannaschii

A D-tagatose 3-epimerase-like protein from M. jannaschii has been crystallized and resolved at 2.64 Å resolution using the SIRAS method.

Azide inhibition of urate oxidase

The structures of azide complexes of urate oxidase are reported.

Cloning, purification, crystallization and preliminary X-ray studies of the putative type VI secretion immunity protein Tli5 (PA5088) from Pseudomonas aeruginosa

Here, the expression, purification, crystallization and preliminary crystallographic analysis of putative protein PA5088 from Pseudomonas aeruginosa are reported.

Expression, purification and crystallization of the phosphate-binding PstS protein from Pseudomonas aeruginosa

The PstS protein from Pseudomonas aeruginosa (PA) has a dual role in bacterial phosphate uptake and extracellular assembly of adhesion fibers. Here the subcloning, expression, isolation, crystallization and crystallographic analysis of native PA PstS are presented.

Crystallization and preliminary crystallographic studies of the complement 1qA globular domain from zebrafish, Dare-C1qAgD

The crystal structure of zebrafish (D. rerio) complement 1qA globular domain (Dare-C1qAgD) was determined from X-ray diffraction data collected to 2.05 Å resolution. The crystal belonged to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 50.347, b = 85.059, c = 95.560 Å.

Crystallization and preliminary X-ray diffraction studies of La1 from Liocheles australasiae

A scorpion venom peptide from L. australasiae was crystallized in space group C2 and an S-SAD data set was collected to 1.9 Å resolution.

Purification, crystallization and preliminary X-ray diffraction analysis of Imp3 in complex with an Mpp10 peptide involved in yeast ribosome biogenesis

An Imp3–Mpp10 complex involved in yeast ribosome assembly has been crystallized.

Preliminary X-ray crystallographic analysis of an engineered glutamyl-tRNA synthetase from Escherichia coli

An engineered version of glutamyl-tRNA synthetase from E. coli (double mutant K236E/E328A) has been crystallized.

Overexpression, purification, crystallization and structure determination of AspB, a putative aspartate aminotransferase from Mycobacterium tuberculosis

Studies pertaining to the overexpression, purification, crystallization and preliminary X-ray work of AspB from M. tuberculosis are reported.

Crystallization and preliminary X-ray analysis of RabX3, a tandem GTPase from Entamoeba histolytica

EhRabX3, a tandem GTPase from Entamoeba histolytica, was cloned, expressed, purified and crystallized. An X-ray diffraction data set was collected from a native crystal to 3.3 Å resolution. The crystal belonged to monoclinic space group C2.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of glyceraldehyde-3-phosphate de­hydrogenase from Streptococcus agalactiae NEM316

The plasminogen binding protein glyceraldehyde-3-phosphate dehydrogenase from S. agalactiae was overexpressed, purified and crystallized, and its preliminary X-ray diffraction analysis is reported at 2.6 Å resolution.

Crystallization and preliminary crystallographic analysis of histamine de­hydrogenase from Natrinema gari BCC 24369

Histamine dehydrogenase isolated from the newly identified halophilic archaeon N. gari BCC 24369 was purified and crystallized. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 211.9, b = 58.6, c = 135.4 Å, β = 103.0°, and diffracted to a resolution of 2.4 Å.

Preliminary X-ray diffraction analysis of a thermophilic β-1,3–1,4-glucanase from Clostridium thermocellum

The catalytic domain of a thermophilic GH16 β-1,3–1,4-glucanase from C. thermocellum has been crystallized and diffracted to a high resolution of 1.95 Å.

Crystallization and preliminary X-ray crystallographic analysis of the C-terminal domain of guanylate kinase-associated protein from Rattus norvegicus

The C-terminal helical domain of GKAP was expressed, purified and crystallized in fusion with maltose-binding protein. Diffraction data were collected to 2.0 Å resolution.

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of the (S)-3-hydroxybutyryl-CoA de­hydrogenase PaaH1 from Ralstonia eutropha H16

The (S)-3-hydroxybutyryl-CoA dehydrogenase PaaH1 from R. eutropha (RePaaH1), an enzyme involved in n-butanol biosynthesis, has been crystallized, and X-ray diffraction data have been collected and analyzed.

Cloning, overexpression, purification and preliminary X-ray analysis of the protein kinase domain of enhanced disease resistance 1 (EDR1) from Arabidopsis thaliana

EDR1 mediates resistance to biotic and antibiotic insults in A. thaliana. A catalytically inactive form of the catalytic domain was crystallized. The crystals belonged to space group P3₂21, contained two molecules per asymmetric unit and diffracted to 2.5 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of Xyn30D from Paenibacillus barcinonensis

The xylanase Xyn30D from P. barcinonensis was overexpressed, purified and crystallized, and its preliminary X-ray diffraction analysis is reported at 2.3 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of Gos1p, a yeast SNARE protein

Gos1 protein (Golgi SNAP receptor complex member 1) is involved in the SNAREs complexes which are the core machinery driving membrane fusion between cargo-carrying vesicles and their target membranes in the secretory and endocytic pathways in yeast. In this paper, the crystallization method of truncated versions of the Gos1 protein from Saccharomyces cerevisiae is reported and preliminary data on its X-ray analysis are provided.

Crystallization of the carb­oxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp34

The crystallization of three C-terminal fragments of the bacteriophage T4 protein gp34 is reported. Diffraction data have been obtained for three native crystal forms and two selenomethionine derivatives, one of which contained high-quality anomalous signal.

Purification, crystallization and preliminary X-ray crystallographic analysis of the UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase (MurF) from Acinetobacter baumannii

The UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase from A. baumannii has been crystallized and preliminary X-ray diffraction data have been collected.

Purification, crystallization and room-temperature X-ray diffraction of inositol de­hydrogenase LcIDH2 from Lactobacillus casei BL23

The preliminary results of the crystallization and room-temperature diffraction of an inositol dehydrogenase are reported.

Purification, crystallization and preliminary X-ray analysis of a putative nucleotide phospho­hydrolase, YpgQ, from Bacillus subtilis

A putative nucleotide phosphohydrolase, YpgQ, from B. subtilis was expressed, purified and crystallized. The YpgQ crystal diffracted X-rays to a resolution of 2.3 Å.

Crystallization and preliminary analysis of the NqrA and NqrC subunits of the Na⁺-translocating NADH:ubi­quinone oxidoreductase from Vibrio cholerae

The NqrA and NqrC subunits of the respiratory Na⁺-NQR complex were crystallized and analyzed by X-ray crystallography at 1.9 and 1.8 Å resolution, respectively.