Structure of the Bacillus anthracis dTDP-L-rhamnose-biosynthetic enzyme dTDP-4-de­hydrorhamnose reductase (RfbD)

The crystal structure of dTDP-4-dehydro-β-L-rhamnose reductase (RfbD) from Bacillus anthracis was determined at 2.65 Å resolution in complex with NADP⁺. RfbD is the fourth enzyme of the dTDP-β-L-rhamnose-biosynthetic pathway and provides evidence that RfbD homologs in Gram-positive bacteria exhibit key differences in the residues important for oligomerization and metal utilization compared with those from many Gram-negative bacteria.

Crystal structure of a pyridoxal 5′-phosphate-dependent aspartate racemase derived from the bivalve mollusc Scapharca broughtonii

The crystal structure of a pyridoxal 5′-phosphate-dependent aspartate racemase derived from Scapharca broughtonii has been resolved at 1.90 Å resolution.

A low-cost method for visible fluorescence imaging

A very low cost method for carrying out imaging of trace fluorescently labeled protein crystallization plates is described.

Structure of the Bacillus anthracis dTDP-L-rhamnose-biosynthetic enzyme dTDP-4-de­hydrorhamnose 3,5-epimerase (RfbC)

The crystal structure of dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase (RfbC) from B. anthracis was determined at 1.63 Å resolution in complex with dTDP and pyrophosphate. RfbC is the third enzyme of the dTDP-L-rhamnose-biosynthetic pathway and only crystallized in the presence of the other three members of the pathway RfbA, RfbB and RfbD.

Swit_4259, an aceto­acetate de­carboxylase-like enzyme from Sphingomonas wittichii RW1

The acetoacetate decarboxylase-like superfamily contains a subset of proteins that are predicted to be involved in secondary metabolism based on gene context. Swit_4259, a protein of unknown function, belongs to this emerging family V subset, in which the overall fold resembles prototypical acetoacetate decarboxylases, but the active-site architecture leads to a divergence in function.

Crystal structure of an anti-idiotype variable lymphocyte receptor

The crystal structure of an anti-idiotype VLR, VLR39, at 1.5 Å resolution is presented and compared with those of other protein-specific VLRs. Binding studies with purified components confirms a VLR39–Fv interaction.

Meditope–Fab interaction: threading the hole

The structure–affinity relationship of complexes of the cetuximab Fab with meditope peptides modified at Arg8 is investigated.

Crystal structure of the second fibronectin type III (FN3) domain from human collagen α1 type XX

The crystal structure of a fibronectin type III domain from human collagen α1 type XX (residues Pro386–Pro466) was solved at 2.5 Å resolution.

The Pseudomonas syringae pv. actinidiae chemoreceptor protein F (PscF) periplasmic sensor domain: cloning, purification and X-ray crystallographic analysis

The sensor domain of the PscF chemoreceptor from the plant pathogen P. syringae pv. actinidiae has been cloned, expressed, purified and crystallized. Preliminary X-ray diffraction data from native and selenomethionine-labelled protein crystals were collected to 1.46 and 2.46 Å resolution, respectively.

Crystal structure of SAM-dependent methyltransferase from Pyrococcus horikoshii

The crystal structure of SAM-dependent methyltransferase from P. horikoshii in complex with the cofactor SAM was solved by X-ray diffraction. The monomeric structure consists of a Rossmann-like fold (domain I) and a substrate-binding domain (domain II). The cofactor binds at the interface between adjacent subunits.

DNA-binding domain of myelin-gene regulatory factor: purification, crystallization and X-ray analysis. Corrigendum

A corrigendum to the paper by Wu et al. [(2017), Acta Cryst. F73, 393–397] is published.