forthcoming articles in Structural Biology and Crystallization CommunicationsOnline

The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.

See also Forthcoming articles in all IUCr journals.

Crystallization and preliminary X-ray diffraction analysis of SeqA bound to a pair of hemimethylated GATC sites

Y. S. Chung and A. Guarné

Crystallization and preliminary X-ray analysis of vicenisaminyltransferase VinC

E. Nango, A. Minami, T. Kumasaka and T. Eguchi

Synopsis: The crystallization of VinC, a glycosyltransferase involved in the biosynthesis of the antitumour antibiotic vicenistatin, is reported.

Crystallization and preliminary X-ray diffraction experiments of arylmalonate decarboxylase from Alcaligenes bronchisepticus

M. Nakasako, R. Obata, R. Okubo, S. Nakayama, K. Miyamoto and H. Ohta

Synopsis: Crystals of arylmalonate decarboxylase from A. bronchisepticus were obtained which diffracted X-rays to a resolution of at least 3.0 Å.

Expression, purification, crystallization and structure determination of two glutathione S-transferase-like proteins from Shewanella oneidensis

B. Remmerie, K. Vandenbroucke, L. De Smet, W. Carpentier, D. De Vos, J. Stout, J. Van Beeumen and S. N. Savvides

Expression, purification and preliminary diffraction studies of PhnP

K. Podzelinska, S.-M. He, A. Soares, D. Zechel, B. Hove-Jensen and Z. Jia

Expression, purification, crystallization and preliminary X-ray analysis of Rv3117, a probable thiosulfate sulfurtransferase (CysA3) from Mycobacterium tuberculosis

S. J. Witholt, R. Sankaranarayanan, C. R. Garen, M. M. Cherney, L. T. Cherney and M. N. G. James

Synopsis: MtbCysA3, a probable rhodanese-like thiosulfate sulfurtransferase, has been expressed, purified and crystallized. Preliminary X-ray analysis shows a dimer in the asymmetric unit.

Isolation, purification, crystallization and preliminary crystallographic studies of sagitoxin, an oligomeric cardiotoxin from the venom of Naja naja saggitifera

R. Mir, M. Sinha, S. Sharma, N. Singh, P. Kaur, A. Srinivasan and T. P. Singh

Preliminary neutron crystallographic analysis of selectively CH₃-protonated deuterated rubredoxin from Pyrococcus furiosus

K. L. Weiss, F. Meilleur, M. P. Blakeley and D. A. A. Myles

Synopsis: The crystallization and preliminary neutron crystallographic analysis of selectively CH₃-protonated deuterated rubredoxin from P. furiosus is presented. This work represents the first reported use of selectively labeled material for phasing applications using neutron protein crystallography.

Purification, crystallization and preliminary X-ray diffraction analysis of the putative ABC transporter ATP-binding protein from Thermotoga maritima

A. S. Ethayathulla, Y. Bessho, A. Shinkai, B. Padmanabhan, T. P. Singh, P. Kaur and S. Yokoyama

Synopsis: The putative ABC transporter ATP-binding protein TM0222 from T. maritima was cloned, overproduced, purified and crystallized. A complete MAD diffraction data set has been collected to 2.3 Å resolution.

Crystallization and structure determination of the core-binding domain of bacteriophage lambda integrase

H. B. Kamadurai, R. Jain and M. P. Foster

Synopsis: The crystal structure of the core-binding domain of bacteriophage lambda integrase has been determined at 2.0 Å resolution.

Crystallization screening test for the whole-cell project on Thermus thermophilus HB8

H. Iino, H. Naitow, Y. Nakamura, N. Nakagawa, Y. Agari, M. Kanagawa, A. Ebihara, A. Shinkai, M. Sugahara, M. Miyano, N. Kamiya, S. Yokoyama, K. Hirotsu and S. Kuramitsu

Synopsis: Three conventional robots were subjected to a crystallization screening test involving 18 proteins from T. thermophilus HB8 using the sitting- and hanging-drop vapour-diffusion and microbatch methods. The number of diffraction-quality crystals and the amount of time required to obtain visible crystals depended greatly on the robots used. The combined use of different robots, especially for protein samples exhibiting low crystallization success rates, significantly increased the chance of obtaining diffraction-quality crystals.

Expression, crystallization and preliminary X-ray analysis of an outer membrane protein from Thermus thermophilus HB27

A. Brosig, J. Nesper, W. Welte and K. Diederichs

Synopsis: The predicted eight-stranded -barrel outer membrane protein TTC0834 from T. thermophilus HB27 was overexpressed in T. thermophilus HB8 and crystallized. Diffraction data were collected to a maximal resolution of 3.2 Å.

Crystallization and preliminary X-ray structural studies of human prouroguanylin

L. Ito, Y. Hidaka, M. Okumura, H. Konishi and H. Yamaguchi

Crystallization and preliminary crystallographic analysis of merohedrally twinned crystals of MJ0729, a CBS-domain protein from Methanococcus jannaschii

P. Fernández-Millán, D. Kortazar, M. Lucas, M. L. Martínez-Chantar, E. Astigarraga, J. A. Fernández, O. Sabas, A. Albert, J. M. Mato and L. A. Martínez-Cruz

Synopsis: Trigonal crystals of MJ0729 showing different degrees of merohedral twinning that may vary from perfect hemihedral twinning to perfect tetartohedral twinning were obtained upon slight variation of the pH.

Crystallization and preliminary X-ray crystallographic analysis of full-length yeast tropomyosin 2 from Saccharomyces cerevisiae

V. Meshcheryakov, Y. Nitanai, R. Maytum, M. A. Geeves and Y. Maeda

Synopsis: Crystals of full-length yeast tropomyosin 2 from S. cerevisiae have been obtained.

Water-mediated variability in the structure of relaxed-state haemoglobin

P. S. Kaushal, R. Sankaranarayanan and M. Vijayan

Synopsis: Partial dehydration of high-salt horse methaemoglobin crystals tends to shift the structure from the R state to the R2 state, in agreement with previous observations that movements in the molecule resulting from changes in water content mimic those involved in protein action.

Crystallization and preliminary X-ray diffraction analysis of biotin acetyl-CoA carboxylase ligase (BirA) from Mycobacterium tuberculosis

V. Gupta, R. K. Gupta, G. Khare, A. Surolia, D. M. Salunke and A. K. Tyagi

Synopsis: The cloning, purification, crystallization and preliminary X-ray characterization of the crystals of biotin acetyl-CoA carboxylase ligase from M. tuberculosis is reported.

Structure of a SARS coronavirus-derived peptide bound to the human major histocompatibility complex class I molecule HLA-B*1501

G. Røder, O. Kristensen, J. S. Kastrup, S. Buus and M. Gajhede

Synopsis: The three-dimensional structure of a SARS coronavirus-derived peptide, VQQESSFVM, bound to the human major histocompatibility complex (MHC) class I antigen HLA-B*1501 is presented.

Purification, crystallization and preliminary crystallographic analysis of biotin protein ligase from Staphylococcus aureus

N. R. Pendini, S. W. Polyak, G. W. Booker, J. C. Wallace and M. C. J. Wilce

Synopsis: The biotin protein ligase from S. aureus has been overexpressed in E. coli, purified, crystallized by the hanging-drop vapour-diffusion method and analysed using X-ray diffraction.

Cloning, purification and crystallization of MotB, a stator component of the proton-driven bacterial flagellar motor

J. O'Neill and A. Roujeinikova

Synopsis: The cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of H. pylori MotB, a peptidoglycan-binding component of the stator of the bacterial flagellar motor, are reported.

Expression, purification, crystallization and primary diffraction analysis of CapF, a capsular polysaccharide-synthesis enzyme from Staphylococcus aureus

T. Miyafusa, Y. Tanaka, M. Kuroda, T. Ohta and K. Tsumoto

Crystallization and preliminary X-ray diffraction analysis of human chondroadherin

A. Pramhed, L. Addis, V. Tillgren, C. Wenglén, D. Heinegård and D. T. Logan

Crystallization and preliminary X-ray analysis of recombinant Glomerella cingulata cutinase

M.-P. Nyon, D. W. Rice, J. M. Berrisford, H.-Z. Huang, A. J. G. Moir, C. J. Craven, S. Nathan, N. M. Mahadi and F. D. Abu Bakar

Synopsis: Recombinant G. cingulata cutinase has been overexpressed and the protein has been purified and crystallized in the absence or presence of inhibitors.

Structure of an N-terminally truncated selenophosphate synthetase from Aquifex aeolicus

E. Matsumoto, S. Sekine, R. Akasaka, Y. Otta, K. Katsura, M. Inoue, T. Kaminishi, T. Terada, M. Shirouzu and S. Yokoyama

Synopsis: The crystal structure of an N-terminally (25 residues) truncated fragment of selenophosphate synthetase (SPS-N) from Aquifex aeolicus has been determined at 2.0 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of the carbohydrate-recognizing domain (VP8*) of bovine rotavirus strain NCDV

X. Yu, A. Guillon, A. J. Szyczew, M. J. Kiefel, B. S. Coulson, M. von Itzstein and H. Blanchard

Synopsis: NCDV VP8*_64-224 was expressed in E. coli, purified and crystallized in the presence of a sialic acid derivative. X-ray diffraction data were obtained to a resolution of 2.0 Å and the crystallographic structure was determined by molecular replacement.

Crystallization and preliminary X-ray diffraction analysis of the subunit Yke2 of the Gim complex from Saccharomyces cerevisiae

R. Pérez de Diego, M. Ortiz-Lombardía and J. Bravo

Synopsis: Crystallization of the -prefoldin subunit Yke2 is reported. This protein is a novel and potentially important target for anti-cancer therapeutics.

Structure of Deinococcus radiodurans tunicamycin-resistance protein (TmrD), a phosphotransferase

U. Kapp, S. Macedo, D. R. Hall, I. Leiros, S. M. McSweeney and E. Mitchell

Synopsis: The 1.95 Å resolution X-ray crystal structure of tunicamycin-resistance protein (TmrD) from D. radiodurans is described.

Purification, crystallization and preliminary X-ray analysis of CMS1MS2: a cysteine proteinase from Carica candamarcensis latex

M. T. R. Gomes, R. D. Teixeira, H. A. L. Ribeiro, A. P. Turchetti, C. F. Junqueira, M. T. P. Lopes, C. E. Salas and R. A. P. Nagem

Synopsis: CMS1MS2, a cysteine proteinase from C. candamarcensis, displays high amidase activity against the substrate BAPNA. The enzyme was purified and crystallized by the hanging-drop method and preliminary diffraction data were collected to 1.8 Å resolution.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the central zinc-binding domain of the human Mcm10 DNA-replication factor

N. Y. Jung, W. J. Bae, J. H. Chang, Y. C. Kim and Y. Cho

Synopsis: Mcm10 is a highly conserved nuclear protein that plays a key role in the initiation and elongation processes of DNA replication by providing a physical link between the Mcm2-7 complex and DNA polymerases. In this study, the central domain of human Mcm10 was crystallized using the hanging-drop vapour-diffusion method in the presence of PEG 3350.

Crystallization of the focal adhesion kinase targeting (FAT) domain in a primitive orthorhombic space group

A. T. Magis, K. M. Bailey, E. V. Kurenova, J. A. Hernández Prada, W. G. Cance and D. A. Ostrov

Structure of a Trypanosoma brucei /-hydrolase fold protein with unknown function

E. A. Merritt, M. Holmes, F. S. Buckner, W. C. Van Voorhis, E. Quartly, E. M. Phizicky, A. Lauricella, J. Luft, G. DeTitta, H. Neely, F. Zucker and W. G. J. Hol

Synopsis: T. brucei gene Tb10.6k15.0140 codes for an /-hydrolase fold protein of unknown function. The 2.2 Å crystal structure shows that members of this sequence family retain a conserved Ser residue at the expected site of a catalytic nucleophile, but that trypanosomatid sequences lack structural homologs for the other expected residues of the catalytic triad.

Dimerization effect of sucrose octasulfate on rat FGF1

N. Kulahin, V. Kiselyov, A. Kochoyan, O. Kristensen, J. S. Kastrup, V. Berezin, E. Bock and M. Gajhede

Synopsis: The work describes the sucrose octasulfate-mediated dimerization of rat FGF1 by gel-filtration experiments and crystal structure determination.

High-resolution structure of the antibiotic resistance protein NimA from Deinococcus radiodurans

H.-K.S. Leiros, C. Tedesco and S. M. McSweeney

Synopsis: In this paper, the 1.2 Å atomic resolution crystal structure of the 5-nitroimidazole antibiotic resistance protein NimA from Deinococcus radiodurans (DrNimA) is presented.

Expression, purification and preliminary crystallographic analysis of recombinant human small glutamine-rich tetratricopeptide-repeat protein

S. Dutta, M. Kotaka and Y.-J. Tan

Synopsis: The production and crystallization of the tetratricopeptide-repeat domain of human small glutamine-rich tetratricopeptide-repeat protein are reported. A 2.4 Å native diffraction data set has been obtained.

Purification, crystallization and preliminary X-ray crystallographic analysis of Rab27a GTPase in complex with exophilin4/Slp2-a effector

L. M. G. Chavas, K. Ihara, M. Kawasaki, R. Kato, T. Izumi and S. Wakatsuki

Synopsis: The GppNHp-bound Rab27a GTPase in complex with exophilin4/Slp2-a has been purified and crystallized. Preliminary crystallographic analyses have been performed at 1.8 Å resolution.

Purification, crystallization and preliminary crystallographic analysis of DehI, a group I -haloacid dehalogenase from Pseudomonas putida strain PP3

J. W. Schmidberger, J. A. Wilce, A. J. Weightman and M. C. J. Wilce

Synopsis: The -haloacid dehalogenase DehI from P. putida strain PP3 was cloned into a vector with an N-terminal His tag and expressed in E. coli Nova Blue strain. Purified protein was crystallized in a primitive monoclinic form and a complete native data set was collected and analysed.

Purification, crystallization and preliminary X-ray diffraction analysis of adenosine triphosphate sulfurylase (ATPS) from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774

O. Y. Gavel, A. V. Kladova, S. A. Bursakov, J. M. Dias, S. Texeira, V. L. Shnyrov, J. J. G. Moura, I. Moura, M. J. Romão and J. Trincão

Synopsis: Native zinc-containing ATP sulfurylase from D. desulfuricans ATCC 27774 was purified to homogeneity and crystallized. Diffraction data were collected to 2.5 Å resolution.

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