forthcoming articles

The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology Communications.

See also Forthcoming articles in all IUCr journals.

Accepted 23 July 2016

Crystal structure of pyruvate de­carboxylase from Zymobacter palmae


Accepted 19 July 2016

Structural analysis of point mutations at the Vaccinia virus A20/D4 interface

The vaccinia virus D4/A20 complex is the DNA polymerase co-factor. The heterodimer interface has been analysed using 3 new crystal structures of the complex where key residues forming a cation-π interaction have been mutated.

Accepted 19 July 2016

The quorum-quenching lactonase from Geobacillus caldoxylosilyticus: purification, characterization, crystallization and crystallographic analysis

A Lactonase, named GcL, from Geobacillus caldoxylosilyticus has been isolated, purified, characterized, and crystallized. Here we show that GcL is a thermostable enzyme that is extremely proficient to degrade lactones. Its structure is expected to provide insights regarding the catalytic mechanism, and highlight the structural determinants involved in the high proficiency of this enzyme.

Accepted 18 July 2016

Crystal structure of Plasmodium falciparum proplasmepsin IV: the plasticity of proplasmepsins

The study of the crystal structure of proplasmepsin IV from Plasmodium falciparum and its comparison with the structures of its mature enzyme and of other proplasmepsins shows the flexibility that characterize the aspartic protease zymogens from malaria parasites.

Accepted 16 July 2016

pHluorin-assisted expression, purification, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit from the Escherichia coli type I restriction-modification system EcoR124I

The C-terminal domain of the HsdR subunit of the E. coli type I restriction-modification system EcoR124I was purified and crystallized in form of a pHluorin (GFP) fusion protein and X-ray diffraction data were collected to a resolution of 2.45 Å.

Accepted 16 July 2016

Surface-layer protein from Caulobacter crescentus: expression, purification and X-ray crystallographic analysis

The large C-terminal domain from the C. crescentus surface-layer protein RsaA has been crystallized. Initial RsaA crystals have been shown to diffract to ∼2.5 Å resolution.

Accepted 13 July 2016

A putative siderophore-interacting protein from the marine bacterium Shewanella frigidimarina NCIMB400: cloning, expression, purification, crystallization and X-ray diffraction analysis

The gene encoding a putative siderophore-interacting protein (SIP) from the marine bacterium S. frigidimarina was successfully cloned, followed by expression and purification of the gene product. Optimized crystals diffracted to 1.35 Å resolution and preliminary crystallographic analysis is promising with respect to structure determination and increased insight into the poorly understood molecular mechanisms underlying iron acquisition.

Accepted 8 July 2016

Crystal structure of AibC, a reductase involved in alternative de novo isovaleryl coenzyme A biosynthesis in Myxococcus xanthu

The crystal structure of AibC, the terminal reductase in myxococcal de novo isovaleryl coenzyme A biosynthesis, has been determined at 2.55 Å resolution.

Accepted 7 July 2016

Structural analysis of a function-associated loop mutant of the substrate-recognition domain of Fbs1 ubiquitin ligase

The crystal structure of the substrate-recognition domain of an Fbs1 mutant was determined at a resolution of 2.3 Å. Comparison of the wild-type and mutant Fbs1 structures provides insight into the structural features of this carbohydrate-binding site.

Accepted 6 July 2016

Crystal structure of Rv3899c184–410, a hypothetical protein from Mycobacterium tuberculosis

The crystal structure of Rv3899c184–410 was found to contain a compact helix bundle and an α/β/α sandwich folding domain.

Accepted 1 July 2016

Characterization of the NTPR and BD1 interacting domains of the human PICH–BEND3 complex

The interaction between PICH and BEND3 is dissected and the domains involved in their interaction have been crystallized.

Accepted 1 July 2016

Structure of the lutein-binding domain of human StARD3 at 1.74 Å resolution and model of a complex with lutein

The structure of a START-domain protein known to bind lutein in the human retina is reported to an improved resolution limit. Rigid-body docking demonstrates that at least a portion of lutein must protrude from the large tunnel-like cavity characteristic of this helix-grip protein and suggests a mechanism for lutein binding specificity.

Accepted 16 June 2016

Crystal structures of a subunit of the formyl­glycinamide ribonucleotide amidotransferase, PurS, from Thermus thermophilus, Sulfolobus tokodaii and Methanocaldococcus jannaschii

Crystal structures of a subunit of the formylglycinamide ribonucleotide amidotransferase, PurS, from T. thermophilus, S. tokodaii and M. jannaschii are determined and their structural characteristics are analyzed.

Accepted 16 June 2016

Crystal structure of the antigen-binding fragment of a monoclonal antibody specific for the multidrug-resistance-linked ABC transporter human P-glycoprotein

The monoclonal antibody UIC2 binds specifically to the extracellular region of the human P-glycoprotein in a conformationally sensitive manner. The crystal structure of the Fab fragment of UIC2 is reported.


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