The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.
This list will generally be short, as papers in this journal are published online as soon as proofs are returned.
See also Forthcoming articles in all IUCr journals.
Synopsis: Vibrio harveyi -N-acetylglucosaminidase (VhGlcNAcase) is a glycoside hydrolase that degrades chitooligosacharides, yielding N-acetylglucosamine (GlcNAc) as the final product. Single crystals of VhGlcNAcase wild-type and D437A mutant were grown and diffracted to highest resolutions of 2.43 and 2.60 Å, respectively.
Synopsis: This crystallization and data collection for a complex of Reb1 bound to Ter3 DNA are reported.
Synopsis: The therapeutic antibody omalizumab inhibits binding of IgE to its receptors. Two crystal structures of the omalizumab Fab fragment have been determined at 1.9 and 3.0 Å resolution, respectively.
Synopsis: Mutants of Turnip yellow mosaic virus protease/ubiquitin hydrolase designed to prevent self-recognition were produced and crystallized as monomers.
Synopsis: In this review, our current structural understanding of the apicomplexan glideosome and actin regulation is described.
Synopsis: The crystal structure of fructosyl peptide oxidase from Eupenicillium terrenum (EtFPOX) was determined at 1.9 Å resolution. This is the first structure of group I FPOD that prefers -fructosyl peptide substrates.
Synopsis: Psb32 from T. elongatus was expressed using a novel vector library for the generation of superfolder fluorescent fusions proteins. X-ray diffraction data were collected to a resolution of 2.3 Å from a crystal belonging to space group P6122.
Synopsis: The crystal structure of human P-cadherin EC1-EC2 in closed conformation is reported at 1.62 Å resolution, showing a Trp2 double conformation in the binding pocket and a novel monomeric packing arrangement.
Synopsis: A GH12 endoglucanase from A. aculeatus F-50 was expressed in P. pastoris, crystallized and diffracted to a high resolution of 1.6 Å.
Synopsis: HutB from V. cholerae has been cloned, overexpressed, purified and crystallized in monomeric form. Crystals belonging to the space group P43212 produced diffraction data to 2.4 Å resolution.
Synopsis: A neutralizing Fab antibody fragment directed against the Wnt antagonist sclerostin was crystallized and its structure has been determined by molecular replacement.
Synopsis: N-myristoyltransferase (NMT) has been shown to be an attractive target for the development of novel therapeutic agents for the treatment of human African trypanosomiasis (HAT). A fragment library has been screened using NMR spectroscopy and the binding mode of hits confirmed by X-ray crystallography using Leishmania major NMT (LmNMT) as a structural surrogate.
Synopsis: Hookworm platelet inhibitor belongs to the cysteine-rich/antigen-5/pathogenesis-related-1 (CAP) protein family. It has been linked with integrin-antagonist, antiplatelet activity found in extracts of Ancylostoma caninum hookworms.
Synopsis: The production, purification, and crystallization of a recently identified trans-sialidase from Trypanosoma vivax is described. The purified enzyme is active and produces diffraction-quality crystals using the sitting drop vapour diffusion method. The crystals belong to space group P212121 and diffract to 2.5 Å.
Synopsis: Structures of the histidine triad family protein from Entamoeba histolytica were solved in complex with sulfate, AMP or GMP. The three structures show very similar nucleotide poses with the sulfate occupying the same space as the alpha phosphate of the nucleotides.
Synopsis: Uridine diphosphate N-acetylglucosamine pyrophosphorylase (UAP) synthesizes UDP-GlcNAc. Entamoeba histolytica UAP appears to lack an N-terminal extension partially involved in allosteric regulation by small molecule inhibitors of UAP from other parasites.
Synopsis: Structures of aspartate aminotransferases from eukaryotic pathogens were solved in complex with pyridoxal phosphate. The four structures show either the open or the closed conformation of the enzyme. The structures highlight the challenges for structure based design of inhibitors targeting the active site, while showing options for inhibitor design targeting the N-terminal arm.
Synopsis: The structure of a tubulin-binding co-factor from Leishmania major is reported and compared with yeast, plant and human orthologues.
Synopsis: The X-ray structure of the antimalarial drug target dihydroorotate dehydrogenase bound to a potent and selective benzamide based inhibitor shows novel binding site interactions.
Synopsis: The crystal structure of Leishmania braziliensis dihydroorotate dehydrogenase, a potential target for drug development against cutaneous leishmaniasis, is reported.
Synopsis: Compound P131 displays antiparasitic activity in a mouse model of Cryptosporidium infection, validating IMP dehydrogenase (IMPDH) as a drug target. Here we report the structure of the enzyme-substrate-P131 complex at 2.05 Å resolution. The structure is an important step to further refine the design of IMPDH inhibitors.
Synopsis: C. parvum is the primary protozoan parasite from the Cryptosporidium genus responsible for cryptosporidiosis in humans. Here, the 2.0 Å resolution structure of the divalent-cation tolerance protein CutA1 encoded in the genome of C. parvum Iowa II is described.
Synopsis: Parasitic protozoans of the Plasmodium type are responsible for malaria, a disease that kills approximately one child every minute in Africa and 2000 people per day worldwide. Here, the solution structure of the 84-residue BolA protein PFE0790c from the deadliest Plasmodium species, P. falciparum, is reported.
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