forthcoming articles in Acta Crystallographica Section F

The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.

This list will generally be short, as papers in this journal are published online as soon as proofs are returned.

See also Forthcoming articles in all IUCr journals.


Accepted 21 October 2014

Crystallization and preliminary X-ray diffraction analysis of a tri-modular endo-[beta]-1,4-glucanase (Cel5B) from Bacillus halodurans

I. Venditto, H. Santos, J. Sandy, J. Sanchez-weatherby, L. M. A. Ferreira, K. Sakka, C. M. G. A. Fontes and S. Najmudin

Synopsis: The full-length Bacillus halodurans tri-modular endo-[beta]-1,4-glucanase has been crystallized and data were processed to 1.64 Å in the orthorhombic space group P 21 21 2. A molecular replacement solution has been found.


Accepted 21 October 2014

Expression, purification and X-ray crystallographic analysis of the Helicobacter pylori Blood Group Antigen Binding Adhesin BabA

S. Subedi, K. Moonens, E. Romão, A. Lo, G. Vandenbussche, J. Bugaytsova, S. Muyldermans, T. Borén and H. Remaut

Synopsis: Recombinant expression and nanobody aided crystallization of a soluble BabA truncate (BabA25-460) corresponding to the predicted adhesin domain.


Accepted 21 October 2014

Crystallization and preliminary X-ray characterization of the full-length bacteriophytochrome from the plant pathogen Xanthomonas campestris pv. campestris

S. Klinke, L. H. Otero, J. Rinaldi, S. Sosa, B. G. Guimarães, W. E. Shepard, F. A. Goldbaum and H. R. Bonomi

Synopsis: The tetra-domain bacteriophytochrome protein XccBphP from Xanthomonas campestris pv. campestris was overexpressed, purified and crystallized in complex with its cofactor biliverdin. A complete dataset was collected to 3.25 Å resolution in the spacegroup P43212 at the SOLEIL Synchrotron.


Accepted 20 October 2014

Expression, crystallization and preliminary crystallographic study of the functional mutant (N60K) of nonstructural protein 9 from Human coronavirus HKU1

X. Chen, Y. Tan, F. Wang, J. Wang, Q. Zhao, S. Li, S. Fu, C. Chen and H. Yang

Synopsis: This article describes the preliminary crystallographic data of a functional mutant (N60K) of nonstructural protein 9 from Human coronavirus HKU1.


Accepted 20 October 2014

Crystallization and preliminary X-ray diffraction analysis of the Sel1-like repeats of SEL1L

H. Jeong, H. Lee and C. Lee

Synopsis: The central SLR (sel1-like repeat) domain of mouse SEL1L was cloned, expressed, purified and crystallized. X-ray diffraction data were collected to 3.3 Å resolution.


Accepted 16 October 2014

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of PhaA from Ralstonia eutropha

E.-J. Kim and K.-J. Kim

Synopsis: The PhaA protein from R. eutropha (RePhaA), the first enzyme in the polyhydroxyalbutyrate (PHB) biosynthetic pathway, was crystallized and X-ray crystallographic analysis was performed.


Accepted 13 October 2014

Crystallization and preliminary X-ray study of biosynthetic alanine racemase from Pseudomonas aeruginosa PAO1

H. Zhou, Z. Li, G. Zhang, S. Xu, Z. Tang, X. Zhu, H. Dong and J. Ju

Synopsis: Biosynthetic alanine racemase from P. aeruginosa PAO1 was expressed in E. coli and purified. The purified enzyme was crystallized and preliminary X-ray crystallographic analysis was performed.


Accepted 13 October 2014

Optimization of the crystallizability of a single-chain antibody fragment

J. Skerlová, V. Král, M. Fábry, J. Sedlácek, V. Veverka and P. Rezácová

Synopsis: A combination of size-exclusion chromatography, Thermofluor assay and dynamic light scattering was used to find conditions optimal for the stability and oligomeric homogeneity of a single-chain variable antibody fragment, which resulted in significantly improved protein crystallizability.


Accepted 11 October 2014

Crystallization and preliminary crystallographic study of Feline infectious peritonitis virus main protease in complex with an inhibitor

J. Wang, F. Wang, Y. Tan, X. Chen, Q. Zhao, S. Fu, S. Li, C. Chen and H. Yang

Synopsis: This article describes the preliminary crystallographic data of a complex of Feline infectious peritonitis virus main protease with its inhibitor N3.


Accepted 3 October 2014

Crystallization and preliminary X-ray diffraction analysis of the phosphate-binding protein PhoX from Xanthomonas citri

V. R. Pegos, F. Javier Medrano and A. Balan


Accepted 3 October 2014

Crystallization and preliminary crystallographic study of Porcine epidemic diarrhea virus main protease in complex with an inhibitor

Y. Tan, F. Wang, X. Chen, J. Wang, Q. Zhao, S. Li, Z. Wang, S. Fu, C. Chen and H. Yang

Synopsis: This article describes the preliminary crystallographic data of a complex consisting of Porcine epidemic diarrhea virus main protease and its inhibitor N3.


Accepted 30 September 2014

Cloning, expression, purification, crystallization and preliminary X-ray characterization of allantoinase from Bacillus licheniformis ATCC 14580

M. Conejero-Muriel, A. I. Martínez-Gómez, S. Martínez-Rodríguez and J. A. Gavira

Synopsis: The cloning, expression, purification, crystallization and preliminary X-ray data collection of allantoinase from B. licheniformis (AllBali) are presented.


Accepted 30 September 2014

Structure of a bacterial microcompartment shell protein bound to a cobalamin cofactor

M. C. Thompson, C. S. Crowley, J. Kopstein, T. A. Bobik and T. O. Yeates


Accepted 29 September 2014

Use of a novel microtitration protocol to obtain diffraction-quality crystals of 4-hydroxy-2-oxoglutarate aldolase from Bos taurus

A. Huang, E. Baker and K. Loomes

Synopsis: A novel vapour-diffusion crystallization approach was utilized to produce crystals of the enzyme 4-hydroxy-2-oxoglutarate aldolase. This `microtitration' method incorporated ammonium sulfate titration, a miniaturization of the working volume (<10 µl) and a controlled rate of evaporation by utilizing a large reservoir, and yielded crystals with favourable diffraction parameters compared with crystals obtained using the conventional hanging-drop method.


Accepted 29 September 2014

Purification, crystallization and preliminary X-ray diffraction analysis of NADP-dependent glutamate dehydrogenase from Aspergillus niger

P. Prakash, A. S. Walvekar, N. S. Punekar and P. Bhaumik

Synopsis: NADP-dependent glutamate dehydrogenase from A. niger has been crystallized and its preliminary X-ray diffraction analysis at 2.9 Å resolution is reported.


Accepted 25 September 2014

Structure of the methanofuran/methanopterin-biosynthetic enzyme MJ1099 from Methanocaldococcus jannaschii

T. A. Bobik, E. J. Morales, A. Shin, D. Cascio, M. R. Sawaya, M. Arbing, T. O. Yeates and M. E. Rasche

Synopsis: The structure of the methanofuran/methanopterin-biosynthetic enzyme (MJ1099) from M. jannaschii was determined at 1.7 Å resolution using anomalous scattering methods.


Accepted 19 September 2014

Ovine [beta]-lactoglobulin at atomic resolution

G. Kontopidis, A. Nordle Gilliver and L. Sawyer

Synopsis: The 1.12 Å resolution crystal structure of a triclinic form of [beta]-lactoglobulin without ligand from the domestic sheep (O. aries) is reported.


Accepted 19 September 2014

Production, crystallization and preliminary X-ray diffraction of the G[alpha]s [alpha]-helical domain in complex with a nanobody

S. Triest, A. Wohlkönig, E. Pardon and J. Steyaert

Synopsis: A crystallization screen was set up for G[alpha]sAHD in complex with the specifically selected nanobody CA9177. A high-resolution (1.59 Å) data set was collected for one of the crystallization conditions.


Accepted 17 September 2014

Overproduction, crystallization and preliminary X-ray crystallographic analysis of Escherichia coli tRNA N6-threonylcarbamoyladenosine dehydratase

S. Kim, K. Y. Kim, J. K. Park, B. I. Lee, Y.-G. Kim and S. Park

Synopsis: TcdA, a recently characterized E. coli tRNA N6-threonylcarbamoyladenosine dehydratase, previously called CsdL or YgdL, was crystallized in space group P21. Diffraction data were collected to 2.70 Å resolution using synchrotron radiation.


Accepted 16 September 2014

Crystallization and preliminary X-ray crystallographic analysis of an artificial molten-globular-like triosephosphate isomerase protein of mixed phylogenetic origin

V. D. Goyal, P. Yadav, A. Kumar, B. Ghosh and R. D. Makde

Synopsis: The protein ccTIM is an engineered variant of the triosephosphate isomerase (TIM) enzyme and has sequence features from both prokaryotic and eukaryotic members of the TIM protein family. Moreover, it is as active as a native protein despite sharing some biophysical characteristics with a molten globular variant. This inscrutable protein was crystallized using the microbatch method and a full X-ray diffraction data set was collected to 2.2 Å resolution using a synchrotron-radiation source.


Accepted 15 September 2014

New crystal structures of adenylate kinase from Streptococcus pneumoniae D39 in two conformations

T. T. Thach and S. Lee


Accepted 11 September 2014

The structure of rice weevil pectin methylesterase

D. C. Teller, C. A. Behnke, K. Pappan, Z. Shen, J. C. Reese, G. R. Reeck and R. E. Stenkamp


Accepted 10 September 2014

Purification, crystallization and preliminary crystallographic investigation of FrnE, a disulfide oxidoreductase from Deinococcus radiodurans

L. Panicker, H. S. Misra and S. C. Bihani

Synopsis: drFrnE, a disulfide oxidoreductase from D. radiodurans, was crystallized and preliminary diffraction data were obtained. The crystal belonged to space group P21221 and diffracted to 1.8 Å resolution.


Accepted 9 September 2014

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the extracellular olfactomedin domain of gliomedin

H. Han and P. Kursula

Synopsis: Gliomedin has an extracellular domain which mediates interactions between the myelinating Schwann cell and the axonal surface, and plays a role in the development and maintenance of the nodes of Ranvier. Here, the purification and crystallization of this olfactomedin domain are reported.


Accepted 8 September 2014

Structure of type II dehydroquinase from Pseudomonas aeruginosa

S. Reiling, A. Kelleher, M. M. Matsumoto, G. Robinson and O. A. Asojo

Synopsis: The recombinant production and structural analysis of catalytically active type II dehydroquinase from P. aeruginosa are presented.


Accepted 5 September 2014

Structure of an Escherichia coli Hfq-RNA complex at 0.97 Å resolution

E. C. Schulz and O. Barabas

Synopsis: The crystal structure of the RNA chaperone Hfq in complex with RNA is presented at 0.97 Å resolution.


Accepted 5 September 2014

In situ proteolysis, crystallization and preliminary X-ray diffraction analysis of a VHH that binds listeria internalin B

I. Huh, R. Gene, J. Kumaran, C. R. MacKenzie and C. L. Brooks

Synopsis: VHH R303 binds the Listeria virulence factor InlB. Crystals of R303 were obtained following in situ proteolysis with trypsin. Trypsin removed the flexible tag region of the protein, creating a homogeneous protein preparation. The crystals diffracted to 1.3 Å resolution.


Accepted 4 September 2014

Purification, crystallization and preliminary X-ray crystallographic analysis of the flagellar accessory protein FlaH from the methanogenic archaeon Methanocaldococcus jannaschii

V. A. Meshcheryakov, Y.-H. Yoon, H. Matsunami and M. Wolf

Synopsis: The flagellar accessory protein FlaH from M. jannaschii has been purified and crystallized. The crystal structure has been solved using the MAD method.


Accepted 3 September 2014

Crystallization, room-temperature X-ray diffraction and preliminary analysis of Kaposi's sarcoma herpesvirus LANA bound to DNA

J. Hellert, J. Krausze, T. F. Schulz and T. Lührs

Synopsis: Rational mutagenesis of the latency-associated nuclear antigen residues 1008-1146 from Kaposi's sarcoma herpesvirus has allowed its crystallization in complex with DNA. A complete data set has been collected at room temperature at a synchrotron-radiation source.


Accepted 3 September 2014

Purification, crystallization, preliminary X-ray diffraction and molecular-replacement studies of great cormorant (Phalacrocorax carbo) haemoglobin

G. Jagadeesan, P. Malathy, K. Gunasekaran, S. Harikrishna Etti and S. Aravindhan


Accepted 2 September 2014

Crystallization and preliminary X-ray diffraction data of [beta]-galactosidase from Aspergillus niger

A. Rico-Díaz, Á. Vizoso Vázquez, M. E. Cerdán, M. Becerra and J. Sanz-Aparicio

Synopsis: [beta]-Galactosidase from A. niger has been expressed in S. cerevisiae, purified and crystallized in its deglycosylated form. X-ray data have been collected to 1.8 Å resolution.


Accepted 2 September 2014

Purification, crystallization and preliminary X-ray diffraction analysis of pyridoxal kinase from Plasmodium falciparum (PfPdxK)

T. Kronenberger, S. Lunev, C. Wrenger and M. R. Groves

Synopsis: The cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of PdxK from P. falciparum are described.


Accepted 31 August 2014

Optimization of crystallization conditions for biological macromolecules

A. McPherson and B. Cudney

Synopsis: Strategies and procedures for the optimization of macromolecular crystal-growth conditions are surveyed. The objective of optimization is to grow crystals with the greatest degree of perfection and that yield the most accurate and precise X-ray diffraction data.


Accepted 12 August 2014

Crystallization and preliminary X-ray analysis of the hypothetical deaminase RPB_0146 from Rhodopseudomonas palustris HaA2

G. Zhang, D. Yu, G. Yang, H. Dong, T. Zhang and X. Liu

Synopsis: The hypothetical deaminase RPB_0146 from R. palustris HaA2 was expressed in E. coli and purified. Crystallization and preliminarily X-ray crystallographic analysis of the recombinant deaminase were performed.


Accepted 2 August 2014

Crystallization and preliminary X-ray diffraction studies of an L-amino-acid oxidase from Lachesis muta venom

A. Ullah, R. Masood, P. J. Spencer, M. T. Murakami and R. K. Arni

Synopsis: An L-amino-acid oxidase from L. muta venom was crystallized and diffraction data were collected to 3.0 Å resolution.




































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