forthcoming articles in Acta Crystallographica Section F

The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.

This list will generally be short, as papers in this journal are published online as soon as proofs are returned.

See also Forthcoming articles in all IUCr journals.

Accepted 26 May 2015

Cloning, expression, purification and preliminary X-ray diffraction studies of galectin-11 from domestic sheep (Ovis aries)

D. Sakthivel, D. Littler, A. Shahine, S. Troy, M. Johnson, J. Rossjohn, D. Piedrafita and T. Beddoe

Synopsis: The cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of ruminant specific galectin-11 is described

Accepted 26 May 2015

Crystallization and preliminary X-ray diffraction analysis of N-terminal domain of Middle East respiratory syndrome coronavirus nucleocapsid protein

Y.-S. Wang, C.- Chang and M.-H. Hou

Synopsis: Crystals of the N-terminal domain of nucleocapsid protein from Middle East respiratory syndrome coronavirus that diffracted X-rays to a resolution of at least 2.63 Å.

Accepted 26 May 2015

Structure of the human MLH1 N-terminus: implications for predisposition to Lynch syndrome

H. Wu, H. Zeng, R. Lam, W. Tempel, I. D. Kerr and J. Min

Synopsis: The crystal structure of the Human MLH1 N-terminus is reported at 2.30 Å. The overall structure is described along with an analysis of two clinically important mutations.

Accepted 25 May 2015

Phormidium phycoerythrin forms hexamers in crystals: a preliminary crystallographic study

R. R. Sonani, M. Sharma, G. D. Gupta, V. Kumar and D. Madamwar

Synopsis: The preliminary structural analysis of marine phycoerythrin is described, showing distinct sequence features.

Accepted 22 May 2015

Molecular cloning, overexpression, purification, crystallization and preliminary X-ray analysis of a GH43 [beta]-xylosidase from Bacillus licheniformis

J. A. Diogo, L. M. Zanphorlin, H. H. Sato, M. T. Murakami and R. Ruller

Synopsis: A GH43 [beta]-xylosidase from the bacterium B. licheniformis was cloned, overexpressed in E. coli BL21(DE3) cells, purified and crystallized. The crystals belonged to the monoclinic space group C2 and diffracted to 2.49 Å resolution.

Accepted 22 May 2015

Batch crystallization of rhodopsin for structural dynamics using an X-ray free electron laser (X-FEL)

W. Wu, P. Nogly, J. Rheinberger, L. M. Kick, C. Gati, G. Nelson, X. Deupi, J. Standfuss, G. Schertler and V. Panneels

Synopsis: We present a new batch preparation method of high density micron-sized crystals of the G protein-coupled receptor (GPCR) rhodopsin for their use in time-resolved serial femtosecond crystallography (SFX) at a X-ray free electron laser (X-FEL) using a liquid jet.

Accepted 21 May 2015

Structure of the response regulator ChrA in the haem-sensing two-component system of Corynebacterium diphtheriae

A. Doi, H. Nakamura, Y. Shiro and H. Sugimoto

Synopsis: The structure of the full-length response regulator ChrA in an unphosphorylated state revealed a unique linker conformation and an interdomain reorientation.

Accepted 20 May 2015

Purification, crystallization and preliminary crystallographic studies of a putative carbohydrate-binding module from the Ruminococcus flavefaciens FD-1 endoglucanase Cel5A

A. J. Pires, T. Ribeiro, A. Thompson, I. Venditto, V. O. Fernandes, P. Bule, H. Santos, V. D. Alves, V. Pires, L. M. A. Ferreira, C. M. G. A. Fontes and S. Najmudin

Synopsis: A putative carbohydrate-binding module from a modular family 5 glycoside hydrolase from R. flavefaciens FD-1 was purified and crystallized and data were collected from cacodylate-derivative crystals to 1.02 and 1.29 Å resolution.

Accepted 20 May 2015

Purification, crystallization and preliminary X-ray crystallographic analysis of a novel aldo-keto reductase from Thermotoga maritima in complex with NADP+

H. Hou, R. Li, X. Wang, Z. Yuan, X. Liu, Z. Chen and X. Xu

Synopsis: A novel thermostable aldo-keto reductase Tm1743 from Thermotoga maritima was overexpressed with an N-terminal His6 tag, purified and co-crystallized with NADP+. Degradation of the N-terminal vector-derived amino acids was identified by Western blot and Mass Spectrometry analysis.

Accepted 19 May 2015

Crystallization and preliminary X-ray diffraction studies of Mycobacterium tuberculosis [sigma]J

K. Goutam, A. K. Gupta and B. Gopal

Synopsis: The cloning, purification, crystallization and preliminary X-ray diffraction studies of M. tuberculosis [sigma]J are reported. M. tuberculosis [sigma]J is an extracytoplasmic function [sigma] factor that governs the cellular response to oxidative stress.

Accepted 19 May 2015

The MORPHEUS II protein crystallization screen

F. Gorrec

Synopsis: MORPHEUS II is a 96-condition initial crystallization screen formulated de novo. The screen incorporates reagents selected from the Protein Data Bank to yield crystals not observed in traditional conditions. In addition, the formulation facilitate optimization and cryoprotection of crystals.

Accepted 18 May 2015

Use of dynamic light scattering and small angle X-ray scattering to characterize new surfactants in solution conditions for membrane protein crystallization

M. Dahani, L.-A. Barret, S. Raynal, C. Jungas, P. Pernot, A. Polidori and F. Bonneté

Synopsis: Influence of fluorinated segment length on surfactant micelle form factors, intermicellar interaction parameters and surfactant phase diagram in solution conditions for MP crystallization.

Accepted 18 May 2015

Structure of recombinant prolidase from Thermococcus sibiricus in space group P21221

V. Timofeev, E. Slutskaya, M. Gorbacheva, K. Boyko, T. Rakitina, D. Korzhenevskiy, A. Lipkin and V. Popov

Synopsis: The crystal structure of the recombinant prolidase from T. sibiricus was determined by X-ray diffraction to a resolution of 2.6 Å and deposited in the Protein Data Bank.

Accepted 18 May 2015

Production, crystallization and preliminary X-ray analysis of LJL143, a protein from the saliva of the Leishmania vector Lutzomyia longipalpis

A. Kelleher, Z. Liu, C. Seid, B. Zhan and O. A. Asojo

Synopsis: LJL143, a salivary protein from L. longipalpis, was produced using P. pastoris and crystallized in space group P212121.

Accepted 15 May 2015

Structure of human collapsin response mediator protein 1: a possible role of its C-terminal tail

S.-H. Liu, S.-F. Huang, Y.-L. Hsu, S.-H. Pan, Y.-J. Chen and Y.-H. Lin

Synopsis: The crystal structure of a functional C-terminal domain of human collapsin response mediator protein 1 has been determined at 3 Å resolution.

Accepted 13 May 2015

The crystal and solution structure of YdiE from Escherichia coli

K. Nishimura, C. Addy, R. Shrestha, A. R. D. Voet, K. Y. J. Zhang, Y. Ito and J. R. H. Tame

Synopsis: YdiE, a conserved protein apparently involved in haem transport, is one of the smallest proteins produced by E. coli at only 63 residues in length. The N-terminal 20 residues of each chain in the homodimer were disordered, but crystals of the full-length protein allowed structure refinement to 1.5 Å resolution.

Accepted 11 May 2015

Towards Time-Resolved Serial Crystallography in a Microfluidic Device

A. S. Pawate, V. Srajer, J. Schieferstein, S. Guha, R. Henning, I. Kosheleva, M. Schmidt, Z. Ren, P. J. A. Kenis and S. L. Perry

Synopsis: An X-ray compatible microfluidic crystallization platform enables in situ time-resolved serial Laue crystallography.

Accepted 9 May 2015

Do Protein Crystals Nucleate within Dense Liquid Clusters?

D. Maes, M. A. Vorontsova, M. A. C. Potenza, T. Sanvito, M. Sleutel, M. Giglio and P. G. Vekilov

Synopsis: We characterize the evolutions of protein-rich clusters and nucleating crystals by dynamic light scattering (DLS), confocal depolarized dynamic light scattering (cDDLS) and depolarized oblique illumination dark-field microscopy. Our observations indicate that the protein-rich clusters are locations for crystal nucleation, moreover we directly detect newly nucleated crystals within protein-rich clusters.

Accepted 7 May 2015

Expression, purification, crystallization and preliminary crystallographic study of the Aspergillus terreus aromatic prenyltransferase AtaPT

B. Gao, R. Chen, X. Liu, J. Dai and F. Sun

Synopsis: A novel aromatic prenyltransferase from A. terreus, named AtaPT, was found to prenylate diverse novel aromatic compounds. The expression and crystallization of AtaPT are reported here.

Accepted 6 May 2015

The structure of a GFP-based antibody (fluorobody) to TLH, a toxin from Vibrio parahaemolyticus

Y. Chen, X. Huang, R. Wang, S. Wang and N. Shi

Synopsis: The expression, purification and crystal structure of a recombinant fluorobody to TLH are reported.

Accepted 6 May 2015

The structure of nerve growth factor in complex with lysophosphatidylinositol

H.-L. Sun and T. Jiang

Synopsis: The crystal structure of mouse nerve growth factor (NGF) complexed with lysophosphatidylinositol was solved and the structural determinants of NGF for LysoPI molecule recognition were identified. The influence of LysoPI in the interactions between NGF and its two receptors is modelled.

Accepted 5 May 2015

Structural characterization of the principal mRNA-export factor Mex67-Mtr2 from Chaetomium thermophilum

S. Aibara, E. Valkov, M. H. Lamers, L. Dimitrova, E. Hurt and M. Stewart

Synopsis: The crystal structures of the individual domains of the Mex67-Mtr2 complex from C. thermophilum have been determined and their arrangement in solution has been studied by SAXS.

Accepted 3 May 2015

Trace fluorescent labeling for protein crystallization

M. Pusey, J. Barcena, M. Morris, A. Singhal, Q. Yuan and J. Ng

Synopsis: The presence of a covalently bound fluorescent probe at concentrations in the < 0.5% range do not affect the outcomes of macromolecule crystallization screening experiments. Additionally, the fluoresence can be used to determine new, not immediately apparent, lead crystallization conditions.

Accepted 1 May 2015

Synthesis, purification and preliminary crystallographic studies of the C-terminal sterol carrier protein type 2 (SCP-2) domain of human hydroxysteroid de­hydrogenase-like protein 2

Z. Cheng, Y. Li, C. Sui, X. Sun and Y. Xie

Synopsis: The C-terminal sterol carrier protein type 2 (SCP-2) domain of human hydroxysteroid dehydrogenase-like protein 2 (including residues Lys318-Arg416) was produced in E. coli, purified and crystallized, and X-ray diffraction data were collected to 2.10 Å resolution.

Accepted 1 May 2015

Structure of human saposin A at lysosomal pH

C. H. Hill, R. J. Read and J. E. Deane

Synopsis: A 1.8 Å resolution structure of the sphingolipid activator protein saposin A has been determined at pH 4.8, the physiologically relevant lysosomal pH for hydrolase enzyme activation and lipid-transfer activity.

Accepted 14 April 2015

Crystallization and preliminary X-ray crystallographic analysis of a nonstructural protein 15 mutant from Human coronavirus 229E

T. Huo and X. Liu

Synopsis: This article describes preliminary crystallographic data for the I26A/N52A mutant of nonstructural protein 15 from Human coronavirus 229E.

Accepted 24 March 2015

Preliminary crystallographic analysis of the Arabidopsis thaliana BAG5-calmodulin protein complex

B. Cui, S. Fang, Y. Xing, Y. Shen and X. Yang

Synopsis: A. thaliana BAG5 (AtBAG5) and calmodulin were expressed and purified separately and then co-crystallized. The preliminary X-ray diffraction studies of the protein complex are reported in order to provide structural insights into the mode of interaction between AtBAG5 and calmodulin.

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