forthcoming articles in Acta Crystallographica Section F

The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.

This list will generally be short, as papers in this journal are published online as soon as proofs are returned.

See also Forthcoming articles in all IUCr journals.


Accepted 24 January 2015

Crystallization and preliminary X-ray diffraction analysis of a putative carbon-carbon bond hydrolase from Mycobacterium abscessus 103

Z. Zhang, Y.-L. Jiang, Y. Wu and Y.-X. He

Synopsis: The crystallization and preliminary X-ray diffraction analysis of a putative carbon-carbon bond hydrolase from M. abscessus 103 are reported.


Accepted 23 January 2015

Purification, crystallization and preliminary X-ray diffraction analysis of a soluble variant of monoglyceride lipase Yju3p from the yeast Saccharomyces cerevisiae

S. Rengachari, P. Aschauer and M. Oberer

Synopsis: A soluble variant of the monoglyceride lipase Yju3p was successfully expressed, purified and crystallized. Diffraction data were collected to 2.4 Å resolution.


Accepted 21 January 2015

Improved crystallization and diffraction of caffeine-induced death suppressor protein 1 (Cid1)

L. A. Yates, B. P. Durrant, M. Barber, K. Harlos, S. Fleurdepine, C. J. Norbury and R. J. C. Gilbert


Accepted 18 January 2015

Cwp84, a Clostridium difficile cysteine protease, exhibits conformational flexibility in the absence of its propeptide

W. J. Bradshaw, A. K. Roberts, C. C. Shone and K. R. Acharya

Synopsis: Two structures of Cwp84, a cysteine protease from the S-layer of C. difficile, are presented after propeptide cleavage. They reveal the movement of three loops, two in the active-site groove and one on the surface of the lectin-like domain, exposing a hydrophobic pocket.


Accepted 17 January 2015

Crystallization and preliminary X-ray analysis of Rv1674c from Mycobacterium tuberculosis

J. Li, X. Wang, W. Gong, C. Niu and M. Zhang

Synopsis: Rv1674c from M. tuberculosis, a possible DNA-binding sulfurtransferase, was purified and crystallized. The preliminary crystallographic analysis was performed.


Accepted 16 January 2015

The structure of tubulin-binding cofactor A from Leishmania major infers a mode of association during the early stages of microtubule assembly

K. L. Barrack, P. K. Fyfe and W. N. Hunter

Synopsis: The structure of a tubulin-binding co-factor from Leishmania major is reported and compared with yeast, plant and human orthologues.


Accepted 16 January 2015

X-ray structure of Plasmodium falciparum di­hydroorotate de­hydrogenase bound to a potent and selective N-phenyl-benzamide inhibitor reveals novel binding-site interactions

X. Deng, D. Matthews, P. K. Rathod and M. A. Phillips

Synopsis: The X-ray structure of the antimalarial drug target dihydroorotate dehydrogenase bound to a potent and selective benzamide based inhibitor shows novel binding site interactions.


Accepted 15 January 2015

Structures of Escherichia coli tryptophanase in holo and `semi-holo' forms

A. Kogan, L. Raznov, G. Y. Gdalevsky, R. Cohen-Luria, O. Almog, A. H. Parola and Y. Goldgur


Accepted 15 January 2015

Crystallization and preliminary X-ray diffraction analysis of the arginine repressor ArgR from Bacillus halodurans

J. Kang, Y. W. Park, H. K. Yeo and J. Y. Lee

Synopsis: Cubic crystals of ArgR from B. halodurans have been obtained. The diffraction was improved by the dehydration method. X-ray data have been collected to 2.35 Å resolution using synchrotron radiation.


Accepted 15 January 2015

Recombinant production, crystallization and crystal structure determination of di­hydroorotate de­hydrogenase from Leishmania Viannia braziliensis

R. Almeida Garcia Reis, E. Lorenzato, V. C. Silva and M. C. Nonato

Synopsis: The crystal structure of Leishmania braziliensis dihydroorotate dehydrogenase, a potential target for drug development against cutaneous leishmaniasis, is reported.


Accepted 13 January 2015

Purification, identification and preliminary crystallographic studies of an allergenic protein from Solanum melongena

A. Jain and D. M. Salunke

Synopsis: SM80.1, a 7S vicilin from S. melongena, was identified, purified and crystallized. The crystals belonged to space group P6322 and diffracted to 2.21 Å resolution.


Accepted 13 January 2015

Crystallization of two operator complexes from the Vibrio cholerae HigBA2 toxin-antitoxin module

S. Hadzi, A. Garcia-Pino, K. Gerdes, J. Lah and R. Loris

Synopsis: The HigA2 antitoxin and the HigBA2 toxin-antitoxin complex from V. cholerae were crystallized in complex with their operator box.


Accepted 13 January 2015

Production, crystallization and X-ray diffraction analysis of a complex between a fragment of the TssM T6SS protein and a camelid nanobody

V. S. Nguyen, S. Spinelli, A. Desmyter, T. T. H. Lè, C. Kellenberger, E. Cascales, C. Cambillau and A. Roussel

Synopsis: Crystals of a complex between a fragment of TssM obtained by limited proteolysis and a nanobody have been obtained and diffracted to 1.9 Å resolution.


Accepted 13 January 2015

Purification, crystallization and preliminary X-ray diffraction studies of the [beta]-catenin homolog HMP-2 from Caenorhabditis elegans

H.-J. Choi and W. I. Weis

Synopsis: The purification, crystallization and molecular-replacement structure solution of two crystal forms of the [beta]-catenin homolog HMP-2 from C. elegans are described.


Accepted 12 January 2015

Crystallization and preliminary X-ray crystallographic analysis of human myotubularin-related protein 1

S. M. Bong, S. W. Yang, J.-W. Choi, S. J. Kim and B. I. Lee

Synopsis: Human myotubularin-related protein 1 (MTMR1) was crystallized using polyethylene glycol 20 000 as a precipitant. Diffraction data have been collected to 2.0 Å resolution using synchrotron X-rays.


Accepted 11 January 2015

Crystallization and preliminary X-ray diffraction studies of the family 54 carbohydrate-binding module from laminarinase ([beta]-1,3-glucanase) Lic16A of Clostridium thermocellum

Y. A. Kislitsyn, V. R. Samygina, I. A. Dvortsov, N. A. Lunina, I. P. Kuranova and G. A. Velikodvorskaya

Synopsis: The paper describes the purification, crystallization and preliminary structural analysis of ctCBM54.


Accepted 9 January 2015

Crystallization and preliminary X-ray crystallographic studies of dipeptidyl peptidase 11 from Porphyromonas gingivalis

Y. Sakamoto, Y. Suzuki, I. Iizuka, C. Tateoka, S. Roppongi, M. Fujimoto, H. Gouda, T. Nonaka, W. Ogasawara and N. Tanaka

Synopsis: Dipeptidyl peptidase 11 from P. gingivalis (PgDPP11) was crystallized. X-ray diffraction data were collected to 1.82 Å resolution.


Accepted 9 January 2015

Cloning, refolding, purification and preliminary crystallographic analysis of the sensory domain of the Campylobacter chemoreceptor for multiple ligands (CcmL)

M. A. Machuca, Y. C. Liu, S. A. Beckham and A. Roujeinikova

Synopsis: A periplasmic sensory domain of the C. jejuni chemoreceptor for multiple ligands (CcmL) has been purified and co-crystallized with isoleucine by the hanging-drop vapour-diffusion method. A diffraction data set has been collected to 1.3 Å resolution.


Accepted 6 January 2015

Structural analysis of human dual-specificity phosphatase 22 complexed with a phosphotyrosine-like substrate

G. T. Lountos, S. Cherry, J. E. Tropea and D. S. Waugh

Synopsis: The crystal structure of a substrate-trapping mutant of human dual-specificity phosphatase 22 (DUSP22) in complex with the small-molecule substrate 4-nitrophenyl phosphate was determined at 1.34 Å resolution.


Accepted 6 January 2015

Structure of Cryptosporidium IMP de­hydrogenase bound to an inhibitor with in vivo antiparasitic activity

Y. Kim, M. Makowska-Grzyska, S. K. Gorla, D. R. Gollapalli, G. D. Cuny, A. Joachimiak and L. Hedstrom

Synopsis: Compound P131 displays antiparasitic activity in a mouse model of Cryptosporidium infection, validating IMP dehydrogenase (IMPDH) as a drug target. Here we report the structure of the enzyme-substrate-P131 complex at 2.05 Å resolution. The structure is an important step to further refine the design of IMPDH inhibitors.


Accepted 5 January 2015

Latest methods of fluorescence-based protein crystal identification

A. Meyer, C. Betzel and M. Pusey


Accepted 4 January 2015

Crystallization and preliminary X-ray crystallographic analysis of Z-ring-associated protein (ZapD) from Escherichia coli

S. H. Son and H. H. Lee

Synopsis: The E. coli ZapD protein was expressed in E. coli, purified and crystallized using lithium sulfate as a precipitant. X-ray diffraction data were collected to 2.95 Å resolution.


Accepted 1 January 2015

Preliminary X-ray crystallographic analysis of an engineered variant of human chimera-type galectin-3 with a shortened N-terminal domain

A. Flores-Ibarra, F. M. Ruiz, S. Vértesy, S. André, H.-J. Gabius and A. Romero

Synopsis: A variant of human galectin-3 containing sections of the N-terminal domain has been crystallized for the first time. The crystals belonged to the orthorhombic space group P212121 and diffracted to 3.3 Å resolution.


Accepted 1 January 2015

Crystallization and preliminary crystallographic analysis of the putative sugar-binding protein Msmeg_0515 (AgaE) from Mycobacterium smegmatis

F. M. Almourfi, H. F. Rodgers, S. E. Sedelnikova and P. J. Baker

Synopsis: The M. smegmatis gene Msmeg_0515 encoding the AgaE protein has been cloned, expressed in E. coli and purified. Crystals of AgaE have been grown that diffracted to beyond 1.5 Å resolution and belonged to space group P212121 with a single AgaE polypeptide in the asymmetric unit.


Accepted 29 December 2014

Structure of a CutA1 divalent-cation tolerance protein from Cryptosporidium parvum, the protozoal parasite responsible for cryptosporidiosis

G. W. Buchko, J. Abendroth, M. C. Clifton, H. Robinson, Y. Zhang, S. N. Hewitt, B. L. Staker, T. E. Edwards, W. C. Van Voorhis and P. J. Myler

Synopsis: C. parvum is the primary protozoan parasite from the Cryptosporidium species responsible for cryptosporidiosis in humans. Here, the 2.0 Å resolution structure of the divalent-cation tolerance protein CutA1 encoded in the genome of C. parvum Iowa II is described.


Accepted 26 December 2014

Structural basis for the recognition of the scaffold protein Frmpd4/Preso1 by the TPR domain of the adaptor protein LGN

H. Takayanagi, S. Yuzawa and H. Sumimoto


Accepted 24 December 2014

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the effector-interaction domain of the resistance protein RGA5-A from Oryza sativa L. japonica

D. Huang, Y. Zhang, Y. Zhao, J. Liu and Y.-L. Peng

Synopsis: RGA5-A_S, the effector-interaction domain of the resistance protein RGA5-A, was expressed, purified and crystallized, yielding crystals that diffracted to 2.43 Å resolution.


Accepted 23 December 2014

Solution-state NMR structure of the putative morphogene protein BolA (PFE0790c) from Plasmodium falciparum

G. Buchko, A. Yee, A. Semesi, P. J. Myler, C. H. Arrowsmith and R. Hui

Synopsis: Parasitic protozoans of the Plasmodium type are responsible for malaria, a disease that kills approximately one child every minute in Africa and ~2000 people per day worldwide. Here, the solution structure of the 84-residue BolA protein, PFE0790c, from the deadliest Plasmodium species, P. falciparum, is reported.


Accepted 22 December 2014

Structural analysis of ibuprofen binding to human adipocyte fatty-acid binding protein (FABP4)

J. M. González and S. Z. Fisher


Accepted 21 December 2014

The role of flexibility and molecular shape in the crystallization of proteins by surface mutagenesis

Y. D. Devedjiev

Synopsis: The role of surface flexibility and molecular shape in protein crystallization is investigated.


Accepted 20 December 2014

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of tomato [beta]-galactosidase 4

M. Eda, M. Ishimaru and T. Tada

Synopsis: Tomato [beta]-galactosidase 4 has been expressed in P. pastoris, purified and crystallized. Diffraction data have been collected to a resolution of 1.65 Å.


Accepted 18 December 2014

Expression, purification and preliminary crystallographic analysis of a haem-utilizing protein, HutX, from Vibrio cholerae

T. Su, K. Chi, K. Wang, L. Guo and Y. Huang

Synopsis: HutX from V. cholerae was expressed, purified and crystallized. The crystals were of sufficient quality to collect high-resolution data sets and carry out a preliminary X-ray crystallographic analysis.


Accepted 18 December 2014

Structure of the apo anti-influenza CH65 Fab

P. S. Lee, A. J. Arnell and I. A. Wilson


Accepted 18 December 2014

Expression, crystallization and preliminary crystallographic data analysis of VioD, a hydroxylase in the violacein-biosynthesis pathway

T. Ran, M. Gao, Q. Wei, J. He, L. Tang, W. Wang and D. Xu

Synopsis: VioD, a hydroxylase in the violacein-biosynthesis pathway, was crystallized and a complete 1.7 Å resolution data set was collected. The crystal belonged to space group P31, with unit-cell parameters a = b = 90.0, c = 94.5 Å, [alpha] = [beta] = 90, [gamma] = 120°.


Accepted 17 December 2014

Preliminary X-ray analysis of the binding domain of the soybean vacuolar sorting receptor complexed with a sorting determinant of a seed storage protein

N. Maruyama, T. Goshi, S. Sugiyama, M. Niiyama, H. Adachi, K. Takano, S. Murakami, T. Inoue, Y. Mori, H. Matsumura and B. Mikami

Synopsis: The preliminary structural analysis of VSR in complex with the C-terminal sorting determinant of a seed storage protein is reported.


Accepted 17 December 2014

Crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of Paenibacillus barcinonensis xylanase 10C containing the CBM22-1-CBM22-2 tandem

M. Á. Sainz-Polo, B. González, F. J. Pastor and J. Sanz-Aparicio

Synopsis: The P. barcinonensis xylanase 10C N-terminal domain, containing the CBM22-1-CBM22-2 tandem, was overexpressed, purified and crystallized, and its preliminary X-ray diffraction analysis is reported at 2.43 Å resolution.


Accepted 15 December 2014

Recombinant expression, purification, crystallization and preliminary X-ray diffraction analysis of Haemophilus influenzae BamD and BamCD complex

J. Lei, X. Cai, X. Ma, L. Zhang, Y. Li, X. Dong, J. St Geme and G. Meng




































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