The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.
This list will generally be short, as papers in this journal are published online as soon as proofs are returned.
See also Forthcoming articles in all IUCr journals.
Synopsis: The structure of the l-amino acid ligase RizA was determined, at 2.8 Å resolution.
Synopsis: Over 250 proteins have been subjected to a standard dye-based thermal melt analysis, and the results suggest that at least a third of soluble protein samples can be made significantly more stable by changing the composition of their formulation.
Synopsis: The middle of the syneptonemal complex protein 1 (SYCP1), which is composed of a coiled-coil domain, was purified and crystallized. The crystals were found to belong to space group I4 with unit-cell parameters of a = 41.95, b = 41.95, and c = 318.78 Å. The crystals were obtained at 293 K and diffracted to a resolution of 3.0 Å.
Synopsis: The potentially structured core domain of the intrinsically disordered protein (IDP) Knr4, residues 80-340, was expressed and crystallized, using native and selenomethionyl-labelled proteins.
Synopsis: The crystal structure of fusion protein 14-3-3-LKB1 provides a novel potential binding mode of 14-3-3 with its ligands.
Synopsis: High resolution crystal structure of cAMP-dependent Protein Kinase (PKA)
Synopsis: Structure of a eukaryotic GUN4 compared with the prokaryotic GUN4 structures.
Synopsis: The crystal structure of Clostridium acetobutylicum ATCC 824 gene CA_C0359 protein product is structurally similar to YteR, an unsaturated rhamnogalacturonyl hydrolase from Bacillus subtilis str 168. Substrate modeling and electrostatic studies of the active site of the structure of CA_C0359 suggests the protein can now be considered part of the CAZy glycoside hydrolase family 105.
Synopsis: Biochemical characterization and preliminary X-ray crystallographic analysis of branching enzymes from the cyanobacterium Cyanothece sp. ATCC 51142 are reported.
Synopsis: Roquin mediates mRNA degradation by recognizing the constitutive decay element. We determined the crystal structures of ROQ domain of Roquin-2 in the ligand-free and RNA bound forms, where roquin-2 recognized RNA forming stem-loop structure.
Synopsis: V. vulnificus VatD was expressed, purified and crystallized. Diffraction data were collected to 2.60 Å resolution for apo VatD and to 2.03 Å resolution for the VatD-desferrioxamine B-Fe3+ complex.
Synopsis: Crystallization and X-ray crystallographic studies of the archaeal ribosomal protein L11 from M. jannaschii are reported.
Synopsis: in vivo protein crystal formation, isolated and structural analysis by novel highly brilliant XFEL and synchrotron radiation sources.
Synopsis: The details of large crystal preparation of X-2 L110F, a mutated carbohydrate-binding module (CBM) of a bacterial xylanase, in complex with a branched xyloglucan oligosaccharide ligand are described. Details include crystallization, H/D exchange, room-temperature X-ray and neutron data collection, initial joint X-ray/neutron refinement using phenix.refine and initial structure analysis. It is shown that it is possible to grow large crystals from only 7 mg starting material and to obtain high-resolution neutron data in 10 d. This is the first neutron structure of a CBM and detailed analysis will add to the growing understanding of protein-carbohydrate interactions at a molecular level.
Synopsis: The crystallization and X-ray diffraction analysis of RsmA from S. aureus are reported.
Synopsis: CoA binds in an extended conformation to the amino-terminal domain of the -subunit of GTP-specific succinyl-CoA synthetase.
Synopsis: Ectoine synthase from S. alaskensis, which catalyzes the production of the stress protectant ectoine, was crystallized, yielding crystals that diffracted to 1.0 Å resolution.
Synopsis: The cloning, expression and crystallographic studies of SarV from S. aureus are reported.
Synopsis: The crystallization of the enzyme NosA, which catalyzes nosiheptide maturation, is reported.
Synopsis: The crystal structure of a serine protease inhibitor from B. bauhinioides was determined at 1.4 Å resolution and compared with the structures of other Kunitz-type inhibitors, including the closely related dual-specificity inhibitor BbCI.
Synopsis: The crystallization and crystallographic study of an FAD-dependent glucose dehydrogenase from A. terreus are reported.
Synopsis: Three crystal structures of recombinant P. aeruginosa FabF are reported: the apoenzyme, an active-site mutant and a complex with a fragment of a natural product inhibitor. The characterization provides reagents and new information to support antibacterial drug discovery.
Synopsis: The aspartate racemase from L. sakei NBRC 15893 has been crystallized by the sitting-drop vapour-diffusion method. The crystal diffracted to 2.6 Å resolution.
Synopsis: The crystal structure of -carbonic anhydrase, an enzyme present in the periplasm of H. pylori and a potential drug target, is described.
Synopsis: An easy to produce, soluble variant of human glutaminyl cyclase has been validated for NMR and X-ray crystallographic screening of candidate inhibitors for lead development against neurodegenerative disorders.
Synopsis: The cloning, expression, purification, crystallization and crystallographic studies of ruminant-specific galectin-11 are described.
Synopsis: Crystals of the N-terminal domain of the nucleocapsid protein from Middle East respiratory syndrome coronavirus that diffracted X-rays to a resolution of at least 2.63 Å are described.
Synopsis: The crystal structure of the human MLH1 N-terminus is reported at 2.30 Å resolution. The overall structure is described along with an analysis of two clinically important mutations.
Synopsis: Crystallographic analysis of marine phycoerythrin is described, showing distinct sequence features.
Synopsis: A GH43 -xylosidase from the bacterium B. licheniformis was cloned, overexpressed in E. coli BL21(DE3) cells, purified and crystallized. The crystals belonged to the monoclinic space group C2 and diffracted to 2.49 Å resolution.
Synopsis: The halogenase HalY, which is highly similar to PltA involved in pyoluteorin biosynthesis, was expressed in E. coli and purified as a monomer. X-ray diffraction data were collected to a resolution of 1.7 Å from a crystal belonging to space group P21.
Synopsis: The structure of the full-length response regulator ChrA in an unphosphorylated state reveals a unique linker conformation and an interdomain reorientation.
Synopsis: A putative carbohydrate-binding module from a modular family 5 glycoside hydrolase from R. flavefaciens FD-1 was purified and crystallized, and data were collected from cacodylate-derivative crystals to 1.02 and 1.29 Å resolution.
Synopsis: Crystallographic X-ray diffraction studies of M. tuberculosis J are reported. M. tuberculosis J is an extracytoplasmic function factor that governs the cellular response to oxidative stress.
Synopsis: The crystal structure of recombinant prolidase from T. sibiricus was determined by X-ray diffraction to a resolution of 2.6 Å and deposited in the Protein Data Bank.
Synopsis: The crystal structure of a functional C-terminal domain of human collapsin response mediator protein 1 has been determined at 3 Å resolution.
Synopsis: This article describes preliminary crystallographic data for the I26A/N52A mutant of nonstructural protein 15 from Human coronavirus 229E.
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