forthcoming articles in Acta Crystallographica Section F

The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.

This list will generally be short, as papers in this journal are published online as soon as proofs are returned.

See also Forthcoming articles in all IUCr journals.


Accepted 20 August 2014

Crystallization and preliminary X-ray diffraction analysis of cyclolavandulyl diphosphate synthase, a new member of the cis-isoprenyl diphosphate synthase superfamily

T. Tomita, T. Ozaki, K. Matsuda, M. Nishiyama and T. Kuzuyama

Synopsis: Cyclolavandulyl diphosphate synthase, which catalyzes both the condensation of two molecules of C5 dimethylallyl diphosphate and the subsequent cyclization, has been crystallized, and X-ray diffraction data have been collected and analyzed.


Accepted 18 August 2014

Expression, Crystallization and Preliminary X-ray Analysis of McbB, a Multi-functional Enzyme Involved in the beta-Carboline Skeleton Biosynthesis

H. Wang, H. Zhang, Y. Mi, J. Ju, Q. Chen and H. Zhang

Synopsis: McbB, a multi-functional enzyme responsible for catalysing Pictet-Spengler (PS) cyclization, decarboxylation, and oxidation reactions in the biosynthesis of [beta]-carboline, was expressed and crystallized. The crystals belong to monoclinic space group P21 with unit-cell parameters a = 66.06 Å, b = 85.48 Å, c = 106.19 Å, [alpha] = 90.00°, [beta] = 106.77°, [gamma] = 90.00°.


Accepted 18 August 2014

Structure of homoserine O-acetyltransferase from Staphylococcus aureus: the first Gram-positive ortholog structure

B. Thangavelu, A. Pavlovski and R. Viola


Accepted 18 August 2014

Crystallization and preliminary X-ray diffraction analyses of the redox-controlled complex of terminal oxygenase and ferredoxin components in the Rieske nonhaem iron oxygenase, carbazole 1,9a-dioxygenase

J. Matsuzawa, H. Aikawa, T. Umeda, Y. Ashikawa, C. Suzuki-Minakuchi, Y. Kawano, Z. Fujimoto, K. Okada, H. Yamane and H. Nojiri

Synopsis: A complex crystal was obtained between reduced terminal oxygenase and oxidized ferredoxin components of carbazole 1,9a-dioxygenase. The crystal belongs to space group P21 and diffracted to 2.25 Å resolution.


Accepted 14 August 2014

Crystallization and preliminary X-ray diffraction analysis of Lin1840 protein, a putative [beta]-glucosidase from Listeria innocua.

M. Nakajima, R. Yoshida, A. Miyanaga and H. Taguchi

Synopsis: Lin1840 protein from Listeria innocua has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to a resolution of 1.8 Å.


Accepted 13 August 2014

Crystallization of the Ets1-Runx1-CBF[beta]-DNA complex formed on the TCR[alpha] gene enhancer

M. Shiina, K. Hamada, T. Inoue-Bungo, M. Shimamura, S. Baba, K. Sato and K. Ogata

Synopsis: The Ets1-Runx1-CBF[beta]-DNA complex, a higher-order TF-DNA complex formed on the T-cell receptor (TCR) [alpha] gene enhancer, was crystallized.


Accepted 13 August 2014

Crystallization and preliminary X-ray crystallographic studies of CrArsM, an As(III) S-adenosylmethionine methyltransferase, from Chlamydomonas reinhardtii

C. Packianathan, J. K. Pillai, A. Riaz, P. Kandavelu, B. Sankaran and B. P. Rosen

Synopsis: The crystallization and preliminary crystallographic analysis of CrArsM, the As(III) S-adenosylmethionine (SAM) methyltransferase from Chlamydomonas reinhardtii, is described.


Accepted 13 August 2014

Crystallization and preliminary X-ray diffraction analysis of the BRPF1 bromodomain in complex with its H2AK5ac and H4K12ac histone peptide ligands

M. Y. Lubula, A. Poplawaski and K. C. Glass


Accepted 13 August 2014

Cloning, expression, purification and preliminary X-ray analysis of EstN2, a novel archaeal [alpha]/[beta]-hydrolase from Candidatus Nitrososphaera gargensis

H. Kaljunen, J. Chow, W. R. Streit and J. Mueller-Dieckmann

Synopsis: The chemolithoautotrophic nitrifying Thaumarchaeon Candidatus Nitrososphaera gargensis with unusual metabolic pathways was enriched by cultivation over 6 years. A novel [alpha]/[beta]-hydrolase was identified, isolated and crystallized for further functional analysis.


Accepted 12 August 2014

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of fructose-1,6-bisphosphate aldolase from Escherichia coli

L. Zhang, Z. Guo, J. Huang, M. Liu, Y. Wang and C. Ji

Synopsis: FBPA I from E. coli was expressed, purified and crystallized. The crystals diffracted to 2.0 Å resolution and belonged to space group C2.


Accepted 12 August 2014

Crystallization and preliminary X-ray analysis of the hypothetical deaminase RPB_0146 from Rhodopseudomonas palustris HaA2

G. Zhang, D. Yu, G. Yang, H. Dong, T. Zhang and X. Liu

Synopsis: Hypothetical deaminase RPB_0146 from R. palustris HaA2 was expressed in E. coli and purified. Crystallization and preliminarily X-ray crystallographic analysis were performed for the recombinant deaminase.


Accepted 12 August 2014

A Drunken Search in Crystallization Space

V. J. Fazio, T. S. Peat and J. Newman

Synopsis: We look to the PDB to find data to answer the question "How well do our current screens cover crystallization space?" and to try to find answers to the more general question about the most efficient strategy to employ in a crystallization campaign.


Accepted 11 August 2014

Purification and X-ray crystallographic analysis of 7-keto-8-aminopelargonic acid (KAPA) synthase from Mycobacterium smegmatis

S. Fan, D. Li, J. Fleming, Y. Hong, T. Chen, L. Zhou, L. Bi, D. Wang, X. Zhang and G. Chen

Synopsis: The cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of 7-keto-8-aminopelargonic acid (KAPA) synthase from M. smegmatis are described..


Accepted 7 August 2014

Human carbonic anhydrase II-cyanate inhibitor complex: putting the debate to rest

D. West, M. A. Pinard, C. Tu, D. N. Silverman and R. McKenna

Synopsis: A controversy has existed in the literature between crystallographic and spectroscopic data on the binding mode of cyanate to carbonic anhydrase II (CA II). To settle this ambiguity, the X-ray crystal complex structures of wild-type and variant V207I CA II with cyanate were redetermined to 1.7 and 1.5 Å resolution, respectively. The data clearly support that cyanate binds directly to the catalytic zinc and not as an outer sphere ligand mimicking the binding of CO2.


Accepted 5 August 2014

Expression, crystallization and preliminary X-ray crystallographic analysis of cystathionine [beta]-lyase from Acinetobacter baumannii OXA-23

D.-Q. Nguyen, H.-P.-T. Ngo, Y.-J. Ahn, S. H. Lee and L.-W. Kang

Synopsis: Cystathionine [beta]-lyase (CBL) from multidrug-resistant A. baumannii OXA-23 (Ab), an enzyme involved in the methionine-metabolism pathway and a novel antibacterial drug target, was cloned, expressed, purified and crystallized. We performed a preliminary X-ray crystallography to analyse the AbCBL crystal.


Accepted 4 August 2014

Expression, purification, crystallization and preliminary X-ray diffraction analysis of acylpeptide hydrolase from Deinococcus radiodurans

V. N. Are, B. Ghosh, A. Kumar, P. Yadav, D. Bhatnagar, S. N. Jamdar and R. D. Makde

Synopsis: The expression, purification and crystallization of acylpeptide hydrolase from D. radiodurans (GenBank accession No. NP_293889) and its preliminary X-ray diffraction analysis to 2.6 Å resolution are reported.


Accepted 2 August 2014

Crystallization and preliminary X-ray diffraction studies of an L-amino-acid oxidase from Lachesis muta venom

A. Ullah, R. Masood, P. J. Spencer, M. T. Murakami and R. K. Arni

Synopsis: An L-amino-acid oxidase from L. muta venom was crystallized and diffraction data were collected to 3.0 Å resolution.


Accepted 2 August 2014

Cloning, expression, purification, crystallization and preliminary X-ray diffraction of a lysine-specific permease from Pseudomonas aeruginosa

E. D. Nji, D. Li, D. A. Doyle and M. Caffrey

Synopsis: The integral membrane lysine permease from P. aeruginosa was cloned and overexpressed in E. coli as a GFP-fusion protein. Crystals of the tag-less transporter diffracted to 7.5 Å resolution.


Accepted 1 August 2014

The structure of alanine racemase from Acinetobacter baumannii

E. Davis, E. Scaletti-Hutchinson, H. Opel-Reading, Y. Nakatani and K. Krause


Accepted 31 July 2014

Crystallization and preliminary X-ray crystallographic analysis of the PH-GRAM domain of human MTMR4

J. U. Lee, J. Y. Son, K.-Y. Yoo, W. Shin, D.-W. Im, S. J. Kim, S. E. Ryu and Y.-S. Heo

Synopsis: The PH-GRAM domain of human MTMR4 was cloned, expressed, purified and crystallized. X-ray diffraction data were collected to 3.20 Å resolution.


Accepted 30 July 2014

Crystallization and preliminary X-ray analysis of two variants of the Escherichia coli O157 ParE2-PaaA2 toxin-antitoxin complex

Y. G. J. Sterckx, S. Haesaerts, L. Van Melderen and R. Loris

Synopsis: A new crystal form of the complex of E. coli O157 PaaA2 and ParE2 is described. The crystals belonged to space group P41212 (or P43212) and diffracted to 2.8 Å resolution. SeMet-derivatized crystals of the complex diffracting to the same resolution were successfully obtained. Finally, crystallization of the complex of a PaaA2 truncation and ParE2 is also described.


Accepted 29 July 2014

Crystallization and preliminary X-ray diffraction analysis of the Pax9 paired domain bound to a DC5 enhancer DNA element

K. Narasimhan, A. Hilbig, B. Udayasuryan, S. Jayabal, P. R. Kolatkar and R. Jauch

Synopsis: Crystals of the Pax9 paired domain bound to a DC5 enhancer DNA element that diffract anisotropically to 3.0 Å resolution are reported.


Accepted 29 July 2014

Structure of Mycobacterium smegmatis Eis in complex with paromomycin

K. H. Kim, D. R. An, H. J. Yoon, J. K. Yang and S. W. Suh

Synopsis: The mycobacterial Eis proteins are aminoglycoside acetyltransferases. Here, the crystal structure of M. smegmatis Eis in the paromomycin-bound form is reported at 3.3 Å resolution.


Accepted 29 July 2014

Recombinant expression, purification, crystallization and preliminary X-ray diffraction analysis of the C-terminal DUF490963-1138 domain of TamB from Escherichia coli

I. Josts, R. Grinter, S. M. Kelly, K. Mosbahi, A. Roszak, R. Cogdell, B. O. Smith, O. Byron and D. Walker

Synopsis: The C-terminal DUF490963-1138 domain of TamB (previously YtfN) from E. coli strain K-12 has been overexpressed, purifed and crystallized. Preliminary diffraction data have been collected to 2.1 Å resolution.


Accepted 29 July 2014

Purification, crystallization and preliminary X-ray crystallographic analysis of TssL from Vibrio cholerae

J.-H. Jeong, J. H. Chang and Y. Kim

Synopsis: The crystallization of TssL from V. cholerae is reported.


Accepted 28 July 2014

Crystallization and preliminary X-ray diffraction analysis of Xaa-Pro dipeptidase from Xanthomonas campestris

A. Kumar, V. N. Are, B. Ghosh, U. Agrawal, S. N. Jamdar, R. D. Makde and S. M. Sharma

Synopsis: The expression, purification and crystallization of Xaa-Pro dipeptidase from X. campestris (GenBank accession No. NP_637763) and its preliminary X-ray diffraction analysis to 1.83 Å resolution are reported.


Accepted 24 July 2014

Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the chicken CD8[alpha][alpha]-BF2*0401 complex

Y. Liu, R. Chen, M. Tariq and C. Xia

Synopsis: A crystal of the chicken MHC class I molecule BF2*0401 in complex with chicken CD8[alpha][alpha] (CD8[alpha][alpha]-BF2*0401) diffracted to 2.8 Å resolution and belonged to space group P21, with unit-cell parameters a = 90.6, b = 90.8, c = 94.9 Å, [beta] = 98°.


Accepted 24 July 2014

Structure of Toxoplasma gondii fructose-1,6-bisphosphate aldolase

L. E. Boucher and J. Bosch

Synopsis: The structure of T. gondii fructose-1,6-bisphosphate aldolase, a glycolytic enzyme and structural component of the invasion machinery, was determined to a resolution of 2.0 Å.


Accepted 23 July 2014

Purification, crystallization and preliminary crystallographic analysis of the 16S rRNA methyltransferase RsmI from Escherichia coli

M. Zhao, L. Wang, H. Zhang, Y. Dong, Y. Gong, L. Zhang and J. Wang

Synopsis: Preliminary crystallographic analysis of the 16S rRNA methyltransferase RsmI from E. coli is reported.


Accepted 23 July 2014

X-ray structures of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana

T. Roret, H. Pégeot, J. Couturier, G. Mulliert, N. Rouhier and C. Didierjean

Synopsis: Cysteine desulfurases (CDs) are enzymes that provide sulfur for several biological processes, including the synthesis of iron-sulfur clusters. The difference in the capacity of the two existing groups of CDs to interact with various partners seems to depend on the flexibility of an extended lobe that harbours the catalytic cysteine.


Accepted 22 July 2014

Purification, crystallization and preliminary X-ray crystallographic analysis of human IL-18 and its extracellular complexes

T. Kimura, N. Tsutsumi, K. Arita, M. Ariyoshi, H. Ohnishi, N. Kondo, M. Shirakawa, Z. Kato and H. Tochio

Synopsis: IL-18 and its two receptors, IL-18R[alpha] and IL-18R[beta], were purified for crystallization and biochemical analysis. IL-18 was crystallized in free, IL-18R[alpha]-bound and IL-18R[alpha]/IL-18R[beta]-bound states and complete X-ray data sets suitable for further structural analysis were obtained from each crystal.


Accepted 22 July 2014

Crystallization and preliminary X-ray analysis of the C-terminal fragment of Ski7 from Saccharomyces cerevisiae

J.-Y. Lee, S. H. Park, B.-C. Jeong and H. K. Song

Synopsis: C-terminal domains 2 and 3 of Ski7 from S. cerevisiae were purified and crystallized. The crystals diffracted to 2.0 Å resolution.


Accepted 15 July 2014

Structure of glutathione S-transferase 1 from the major human hookworm parasite Necator americanus (Na-GST-1) in complex with glutathione

O. A. Asojo and C. Ceccarelli

Synopsis: The structure of Na-GST-1 in complex with GST is reported.


Accepted 15 July 2014

Structure of a DsbF homologue from Corynebacterium diphtheriae

S.-H. Um, J.-S. Kim, K. Lee and N.-C. Ha


Accepted 15 July 2014

Expression, purification, crystallization and preliminary X-ray analysis of glucose-1-phosphate uridylyltransferase (GalU) from Erwinia amylovora

M. Toccafondi, M. Cianci and S. Benini

Synopsis: The glucose-1-phosphate uridylyltransferase from E. amylovora has been cloned, expressed, purified and crystallized. Data were collected to 2.46 Å resolution in space group P62 and the structure was solved by molecular replacement using the structure of the enzyme from E. coli.


Accepted 11 July 2014

Carboplatin binding to histidine

S. W. M. Tanley, K. Diederichs, L. M. J. Kroon-Batenburg, C. Levy, A. M. M. Schreurs and J. R. Helliwell

Synopsis: An X-ray crystal structure showing the binding of purely carboplatin to histidine in a model protein has finally been obtained. This required extensive crystallization trials and various novel crystal structure analyses.


Accepted 10 July 2014

Expression, purification and preliminary crystallographic analysis of the cryptic polo-box domain of Caenorhabditis elegans ZYG-1

E. Shimanovskaya and G. Dong

Synopsis: The cryptic polo-box (CPB) domain of the polo-like kinase ZYG-1 from C. elegans was cloned, overexpressed, purified and crystallized. Anomalous X-ray diffraction data were collected to 2.54 Å resolution.


Accepted 8 July 2014

Purification, characterization and preliminary X-ray crystallographic studies of monodehydroascorbate reductase from Oryza sativa L. japonica

H. Do, I.-S. Kim, Y.-S. Kim, S.-Y. Shin, J.-J. Kim, J.-E. Mok, S.-I. Park, A. R. Wi, H. Park, J. H. Lee, H.-S. Yoon and H.-W. Kim

Synopsis: Monodehydroascorbate reductase (OsMDHAR) from O. sativa L. japonica was expressed as the active form using E. coli strain NiCo21 (DE3). X-ray diffraction data were collected to 1.9 Å resolution.


Accepted 5 July 2014

Crystallization of the C-terminal redox domain of the sulfur-assimilatory enzyme APR1 from Arabidopsis thaliana

F.-F. Chen, Y.-Y. Chang, C.-C. Cho and C.-H. Hsu

Synopsis: The redox domain of APR1 from A. thaliana was crystallized in the tetragonal space group P43212 or P41212 and diffraction data were collected to 2.70 Å resolution.


Accepted 5 July 2014

Crystallization and preliminary X-ray crystallographic analysis of YfcM: an important factor for EF-P hydroxylation

K. Kobayashi, T. Suzuki, N. Dohmae, R. Ishitani and O. Nureki

Synopsis: E. coli YfcM was expressed, purified and crystallized. Crystals of YfcM were obtained by the in situ proteolysis crystallization method. Using these crystals, an X-ray diffraction data set was collected at 1.45 Å resolution.


Accepted 5 July 2014

Expression, purification, crystallization and preliminary crystallographic analysis of human myotubularin-related protein 3

J. Y. Son, J. U. Lee, K.-Y. Yoo, W. Shin, D.-W. Im, S. J. Kim, S. E. Ryu and Y.-S. Heo

Synopsis: Human myotubularin-related protein 3 was cloned, expressed, purified and crystallized. X-ray diffraction data were collected to 3.3 Å resolution.


Accepted 3 July 2014

Structure of nucleoside diphosphate kinase from pacific shrimp (Litopenaeus vannamei) in binary complexes with purine and pyrimidine nucleoside diphosphates

A. A. López-Zavala, I. E. Quintero-Reyes, J. S. Carrasco-Miranda, V. Stojanoff, A. Weichsel, E. Rudiño-Piñera and R. R. Sotelo-Mundo


Accepted 2 July 2014

High-resolution crystal structures of alternate forms of the human CD44 hyaluronan-binding domain reveal a site for protein interaction

L.-K. Liu and B. Finzel

Synopsis: Two alternate crystal forms of the human CD44 hyaluronan-binding domain, one hexagonal and one monoclinic, have been determined at 1.60 and 1.08 Å resolution, respectively. A short peptide found in the monoclinic form may identify a site of CD44 for protein-protein interactions.


Accepted 2 July 2014

Cloning, purification, crystallization and preliminary X-ray studies of a carbohydrate-binding module (CBM_E1) derived from a sugarcane soil metagenome

B. M. Campos, T. M. Alvarez, M. V. Liberato, I. Polikarpov, H. J. Gilbert, A. C. M. Zeri and F. M. Squina


Accepted 2 July 2014

Microseed matrix screening for optimization in protein crystallization: what have we learned?

A. D'Arcy, T. Bergfors, S. W. Cowan-Jacob and M. Marsh

Synopsis: Microseed matrix screening is increasingly successful in the optimization of protein crystallization leads.


Accepted 1 July 2014

Cloning, purification and preliminary crystallographic analysis of the Helicobacter pylori pseudaminic acid biosynthesis N-acetyltransferase PseH

Y. C. Liu, A. I. Ud-Din and A. Roujeinikova

Synopsis: The N-acetyltransferase PseH from the H. pylori pseudaminic acid biosynthesis pathway has been purified and co-crystallized with acetyl-CoA by the hanging-drop vapour-diffusion method. A diffraction data set has been collected to 2.5 Å resolution.


Accepted 26 June 2014

Structural basis for the inhibition of poly(ADP-ribose) polymerases 1 and 2 by BMN 673, a potent inhibitor derived from dihydropyridophthalazinone

M. Aoyagi-Scharber, A. S. Gardberg, B. K. Yip, B. Wang, Y. Shen and P. A. Fitzpatrick

Synopsis: BMN 673, a novel PARP1/2 inhibitor in clinical development with substantial tumor cytotoxicity, forms extensive hydrogen-bonding and [pi]-stacking in the nicotinamide pocket, with its unique disubstituted scaffold extending towards the less conserved edges of the pocket. These interactions might provide structural insight into the ability of BMN 673 to both inhibit catalysis and affect DNA-binding activity.


Accepted 23 June 2014

Structural analysis of the GH43 enzyme Xsa43E from Butyrivibrio proteoclasticus

M. Till, D. Goldstone, G. Card, G. T. Attwood, C. D. Moon and V. L. Arcus


Accepted 23 June 2014

Crystallization and preliminary X-ray diffraction analysis of the periplasmic domain of the Escherichia coli aspartate receptor Tar and its complex with aspartate

T. Mise, H. Matsunami, F. Samatey and I. Maruyama


Accepted 21 June 2014

Crystallization and preliminary X-ray analysis of the periplasmic domain of FliP, an integral membrane component of the bacterial flagellar type III protein-export apparatus

T. Fukumura, Y. Furukawa, T. Kawaguchi, Y. Saijo-Hamano, K. Namba, K. Imada and T. Minamino

Synopsis: The periplasmic domain of FliP of the bacterial flagellar type III export apparatus has been expressed, purified and crystallized, and the crystals have been characterized by X-ray diffraction.


Accepted 21 June 2014

Cloning, expression, purification, crystallization and preliminary crystallographic analysis of the C-terminal domain of Par-4 (PAWR)

U. K. Tiruttani Subhramanyam, J. Kubicek, U. B. Eidhoff and J. Labahn

Synopsis: The cloning, overexpression, purification, crystallization and preliminary crystallographic analysis of the C-terminal domain of prostate apoptosis response-4 protein (Par-4) are presented.


Accepted 20 June 2014

Purification, crystallization and preliminary X-ray crystallographic analysis of glycosyltransferase-1 from Bacillus cereus

Y.-C. Hsieh, H.-H. Chiu, Y.-C. Huang, H.-K. Fun, C.-Y. Lu, Y.-K. Li and C.-J. Chen

Synopsis: The purification, crystallization and preliminary X-ray crystallographic analysis of B. cereus glycosyltransferase-1 are reported.


Accepted 18 June 2014

Molecular replacement with a large number of molecules in the asymmetric unit

C. Jobichen and K. Swaminathan

Synopsis: A survey of the Protein Data Bank for entries with a large number of molecules in the asymmetric unit was performed and the strategies used for molecular replacement are described.


Accepted 14 June 2014

Chemical conversion of cisplatin and carboplatin with histidine in a model protein crystallized under sodium iodide conditions

S. W. M. Tanley and J. R. Helliwell

Synopsis: Crystals of HEWL with cisplatin and HEWL with carboplatin grown in sodium iodide conditions both show a partial chemical transformation of cisplatin or carboplatin to a transiodoplatin (PtI2X2) form. The binding is only at the N[delta] atom of His15. A further Pt species (PtI3X) is also seen, in both cases bound in a crevice between symmetry-related protein molecules.


Accepted 14 June 2014

The binding of platinum hexahalides (Cl, Br and I) to hen egg-white lysozyme and the chemical transformation of the PtI6 octahedral complex to a PtI3 moiety bound to His15

S. W. M. Tanley, L.-V. Starkey, L. Lamplough, S. Kaenket and J. R. Helliwell

Synopsis: The platinum hexahalides have an octahedral arrangement of six halogen atoms bound to a Pt centre, thus having an octahedral shape that could prove to be useful in poor electron-density maps. In a detailed characterization, PtI6 chemically transformed to a square-planar PtI3 complex bound to the N[delta] atom of His15 of HEWL was also observed, which was not observed for PtBr6 or PtCl6.


Accepted 10 June 2014

Crystallization and preliminary X-ray diffraction analysis of the S-adenosylhomocysteine hydrolase (SAHH) from Thermotoga maritima

M. He, Y. Zheng, C.-H. Huang, G. Qian, X. Xiao, T.-P. Ko, W. Shao and R.-T. Guo


Accepted 1 June 2014

Crystallization, preliminary X-ray crystallographic and cryo-electron microscopy analysis of a bifunctional enzyme fucokinase/L-fucose-1-P-guanylyltransferase from Bacteroides fragilis

C. Cheng, J. Gu, J. Su, W. Ding, J. Yin, W. Liang, X. Yu, J. Ma, P. G. Wang, Z. Xiao and Z.-J. Liu

Synopsis: The expression, purification, crystallization, preliminary X-ray crystallographic and cryo-EM analysis of the bifunctional enzyme fucokinase/L-fucose-1-P-guanylyltransferase are reported. A diffraction data set was collected to 3.7 Å resolution from the full-length native protein crystal using synchrotron X-rays. The preliminary cryo-EM image showed two parallel disc-shaped objects with a diameter of 10 nm.




































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