forthcoming articles in Acta Crystallographica Section F

The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.

This list will generally be short, as papers in this journal are published online as soon as proofs are returned.

See also Forthcoming articles in all IUCr journals.

Accepted 27 August 2015

Structural analysis of SepL, an enteropathogenic Escherichia coli type III secretion system gatekeeper protein

B. J. Burkinshaw, S. A. Souza and N. C. J. Strynadka

Synopsis: The X-ray crystal structure of the enteropathogenic Escherichia coli gatekeeper protein SepL has three X-bundle domains.

Accepted 27 August 2015

Structure of a catalytic dimer of the [alpha]- and [beta]-subunits of the F-ATPase from Paracoccus denitrificans at 2.3 Å resolution

E. Morales-Ríos, M. G. Montgomery, A. G. W. Leslie, J. J. García-Trejo and J. E. Walker

Synopsis: The structure has been determined at 2.3 Å resolution of the [alpha]-[beta] hetero-dimer of the F-ATPase from the [alpha]-proteobacterium, Paracoccus denitrificans. It corresponds to the "open" or "empty" catalytic interface found in other F-ATPases.

Accepted 25 August 2015

Extension of resolution and oligomerization state studies of 2,4'-di­hydroxyaceto­phenone dioxygenase from Alcaligenes sp. 4 HAP

J. Guo, P. Erskine, A. R. Coker, J. Gor, S. J. Perkins, S. P. Wood and J. B. Cooper

Synopsis: We have extended the resolution of the structure of the enzyme 2,4'-dihydroxyacetophenone dioxygenase to 1.88 Å and demonstrated by gel-filtration and analytical ultracentrifugation that in-situ chymotrypsinolysis, which is required for crystallization, converts the enzyme from a tetrameric to a dimeric form. A putative model of the tetramer obtaining by symmetric docking studies closely resembles the tetrameric assembly of the homologous rmlc-like cupin protein from R. eutropha: 3ebr.

Accepted 25 August 2015

Structures of exoglucanase from Clostridium cellulovorans: cello­tetraose binding and cleavage

L.-C. Tsai, I. Amiraslanov, H.-R. Chen, Y.-W. Chen, H.-L. Lee, P.-H. Liang and Y.-C. Liaw

Accepted 25 August 2015

An active site-tail interaction in the crystal structure of hexahistidine-tagged Thermoplasma acidophilum citrate synthase

J. R. Murphy, S. Donini and T. J. Kappock

Synopsis: Citrate synthase from the thermophilic euryarchaeon Thermoplasma acidophilum, fused to a hexahistidine tag, was purified and biochemically characterized. The structure of the unliganded enzyme at 2.2 Å resolution contains tail-active site contacts in half of the active sites.

Accepted 21 August 2015

Crystallization and preliminary X-ray analysis of the transcription activator protein C1 of bacteriophage P22 in complex with the PRE promoter element

A. Mondal, R. Chattopadhyaya, A. B. Datta and P. Parrack

Synopsis: Crystallization and preliminary data collection to 2.2 Å for the C1 protein of salmonella phage P22 as a complex with a 23-mer DNA is reported.

Accepted 19 August 2015

Structure of the HECT domain of human WWP2

W. Gong, X. Zhang, W. Zhang, J. Li and Z. Li

Synopsis: The crystal structure of the HECT domain of WWP2, an E3 ubiquitin-protein ligase, has been determined at 2.5 Å resolution and reveals a compact inverted "T" shape conformation.

Accepted 18 August 2015

Purification, crystallization, and X-ray crystallographic analysis of human RAB11(S20V)-a constitutively active GTP-binding form

C. M. Kim, J. Y. Choi and H. H. Park

Synopsis: RAB11(S20V), a constitutively active GTP-binding form of RAB1 1, was purified and crystallized. The crystals belong to space group I4 with unit-cell parameters of a = 74.11, b = 74.11, c = 149.44 Å. They were obtained at 293 K and diffracted to a resolution of 2.4 Å.

Accepted 17 August 2015

Structure and binding properties of a cameloid nanobody raised against KDM5B

A. Wiuf, L. H. Kristensen, O. Kristensen, J. Dorosz, J. Jensen and M. Gajhede

Synopsis: KDM5B is an important new putative cancer therapy target. Here we describe the binding properties and crystal structure of a nanobody raised against KDM5B.

Accepted 17 August 2015

Crystallization and initial crystallographic analysis of covalent DNA-cleavage complexes of Staphyloccocus aureus DNA gyrase with QPT-1, moxifloxacin and etoposide

V. Srikannathasan, A. Wohlkonig, A. Shillings, O. Singh, P. F. Chan, J. Huang, M. N. Gwynn, A. P. Fosberry, P. Homes, M. Hibbs, A. J. Theobald, C. Spitzfaden and B. D. Bax

Synopsis: The antibacterial compound QPT-1, the antibacterial drug moxifloxacin and the anticancer drug etoposide were each co-crystallized with DNA and S. aureus DNA gyrase. The base pairs at the ends of DNA duplexes were modified to optimize the crystal contacts and to give diffraction data to between 2.45 and 3.15 Å resolution from six DNA-complex crystals.

Accepted 14 August 2015

Crystallization and preliminary X-ray diffraction studies of frutalin, an [alpha]-D-galactose-specific lectin from Artocarpus incisa seeds

A. C. O. Monteiro-Moreira, H. D'Muniz Pereira, A. E. Vieira Neto, F. B. Mendes Batista Moreno, M. Duarte Pinto Lobo, F. D. Sousa and R. A. Moreira

Synopsis: Frutalin, an [alpha]-D-galactose-binding lectin of biomedical interest from A. incisa seeds, has been crystallized and its preliminary X-ray diffraction analysis at 1.81 Å resolution is reported.

Accepted 8 August 2015

Analysis of crystallization data in the Protein Data Bank

J. Kirkwood, D. Hargreaves, S. O'Keefe and J. Wilson

Synopsis: In a large-scale study using data from the Protein Data Bank, some of the many reported findings regarding the crystallization of proteins were investigated.

Accepted 6 August 2015

Purification, crystallization, crystallographic analysis and phasing of the CRISPR-associated protein Csm2 from Thermotoga maritima

G. Gallo, G. Augusto, G. Rangel, A. Zelanis, M. A. Mori, C. Barbosa Campos and M. Würtele

Synopsis: Csm2 is a major component of type IIIA CRISPR ribonucleoprotein complexes that confer prokaryotes with immunity against phages and plasmids via an RNA-guided interference mechanism. The Csm2 protein from T. maritima was recombinantly expressed, purified and crystallized, and its structure was solved via cadmium single-wavelength anomalous diffraction phasing.

Accepted 4 August 2015

Structure of the response regulator RPA3017 involved in red-light signaling in Rhodopseudomonas palustris

X. Yang, X. Zeng, K. Moffat and X. Yang

Synopsis: The crystal structure of the response regulator RPA3017 from the photosynthetic bacterium R. palustris has been determined at 1.9 Å resolution. RPA3017 is part of a two-component system that involves two tandem bacteriophytochromes involved in red-light signaling.

Accepted 28 July 2015

Structure of DnmZ, a nitrososynthase in the Streptomyces peucetius anthracycline-biosynthetic pathway

L. Sartor, C. Ibarra, A. Al-Mestarihi, B. O. Bachmann and J. L. Vey

Synopsis: The anthracycline-biosynthetic enzyme DnmZ has been structurally characterized in ligand-free and thymidine diphosphate-bound forms.

Accepted 1 July 2015

Formulation screening by differential scanning fluorimetry: how often does it work?

M. Ristic, N. Rosa, S. A. Seabrook and J. Newman

Synopsis: Over 250 proteins have been subjected to a standard dye-based thermal melt analysis, and the results suggest that at least a third of soluble protein samples can be made significantly more stable by changing the composition of their formulation.

Accepted 27 June 2015

A sucrose-binding site provides a lead towards an isoform-specific inhibitor of the cancer-associated enzyme carbonic anhydrase IX

M. A. Pinard, M. Aggarwal, B. Mahon, C. Tu and R. McKenna

Synopsis: An engineered human carbonic anhydrase IX (CA IX) mimic was co-crystallized with the disaccharide sucrose. Its binding provides a novel `interaction' site that can be used to design targeted inhibitors of CA IX, a key marker of hypoxic tumors.

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