forthcoming articles in Structural Biology and Crystallization CommunicationsOnline

The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.

See also Forthcoming articles in all IUCr journals.

Purification, crystallization and preliminary crystallographic studies of the complex of interferon-1 with its receptor

E. Magracheva, S. Pletnev, S. Kotenko, W. Li, A. Wlodawer and A. Zdanov

Synopsis: Interferon-1 and the extracellular domain of its receptor IFN-1R1 were expressed in insect cells and purified to homogenity. Ligand-receptor complexes of ligands expressed in insect cells and in E. coli were formed, purified by size-exclusion chromatography and crystallized. Preliminary X-ray studies showed that the crystals diffracted to better than 2.2 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of Lon from Thermococcus onnurineus NA1

Y. J. An, C.-R. Lee, S. Supangat, H. S. Lee, J.-H. Lee, S.-G. Kang and S.-S. Cha

Cloning, expression and crystallization of dihydrodipicolinate reductase from methicillin-resistant Staphylococcus aureus

S. Dommaraju, M. A. Gorman, C. Dogovski, F. G. Pearce, J. A. Gerrard, R. C. J. Dobson, M. W. Parker and M. A. Perugini

Synopsis: Given the recent rise in antimicrobial resistance, there is an urgent need to identify and characterize new antibiotic drug targets. One such target is dihydrodipicolinate reductase (DHDPR), which is an essential bacterial enzyme that catalyzes the second step in the lysine-biosynthesis pathway. In this paper, the cloning, expression, purification and crystallization of DHDPR from methicillin-resistant S. aureus are presented.

Crystallization and X-ray diffraction analysis of human CLEC5A (MDL-1), a dengue virus receptor

A. A. Watson and C. A. O'Callaghan

Synopsis: Recombinant human CLEC5A was crystallized in the trigonal space group P3₁ and X-ray diffraction data were collected to 1.56 Å resolution.

Crystallization of dihydrodipicolinate synthase from a clinical isolate of Streptococcus pneumoniae

N. E. Sibarani, M. A. Gorman, C. Dogovski, M. W. Parker and M. A. Perugini

Synopsis: The emergence of drug-resistant bacteria highlights the importance of identifying potential drug targets. Dihydrodipicolinate synthase (DHDPS) is a valid but as yet unexploited antimicrobial target that functions in the biosynthesis of (S)-lysine. In this study, the cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DHDPS from S. pneumoniae are described.

Crystallization and preliminary X-ray diffraction analysis of diaminopimelate epimerase from Escherichia coli

L. Hor, R. C. J. Dobson, C. Dogovski, C. A. Hutton and M. A. Perugini

Synopsis: Diaminopimelate (DAP) epimerase, an enzyme in the lysine-biosynthetic pathway, is a promising target for antibiotic development against pathogenic bacteria. Here, the cloning, expression, purification, crystallization and preliminary diffraction analysis of DAP epimerase from E. coli are reported.

Crystallization and preliminary X-ray crystallographic analysis of the N domain of p97/VCP in complex with the UBX domain of FAF1

H. Y. Shin, W. Kang, S. Y. Lee and J. K. Yang

Synopsis: The N domain of p97/VCP was crystallized in complex with the UBX domain of FAF1. X-ray diffraction data were collected to 2.60 Å resolution and the crystals belonged to space group C222₁.

Crystallization and preliminary X-ray diffraction data of -galactosidase from Saccharomyces cerevisiae

R. Fernández-Leiro, Á. Pereira-Rodríguez, M. E. Cerdán, M. Becerra and J. Sanz-Aparicio

Synopsis: -Galactosidase from S. cerevisiae has been purified and crystallized in glycosylated and deglycosylated states. X-ray diffraction data were collected to 1.95 Å resolution from the deglycosylated form.

Crystallization and preliminary X-ray crystallographic analysis of DNA gyrase GyrB subunit from Xanthomonas oryzae pv. oryzae

H. Y. Jung, K. J. Lee, K. H. Kim, J. H. Hyoung, M. R. Han, H. K. Kim, L.-W. Kang, Y.-J. Ahn and Y.-S. Heo

Synopsis: The GyrB subunit of DNA gyrase from X. oryzae pv. oryzae was expressed, purified and crystallized. Diffraction data were collected to a resolution of 2.10 Å.

Crystallization and preliminary X-ray diffraction data analysis of stenodactylin, a highly toxic type 2 ribosome-inactivating protein from Adenia stenodactyla

G. Tosi, S. Fermani, G. Falini, L. Polito, M. Bortolotti and A. Bolognesi

Purification, crystallization and preliminary X-ray diffraction analysis of Cif, a virulence factor secreted by Pseudomonas aeruginosa

C. D. Bahl, D. P. MacEachran, G. A. O'Toole and D. R. Madden

Synopsis: The P. aeruginosa virulence factor Cif, which inhibits cell-surface expression of the cystic fibrosis transmembrane conductance regulator, has been expressed, purified and crystallized.

Crystallization and preliminary crystallographic characterization of glutamine synthetase from Medicago truncatula

A. R. Seabra, H. Carvalho and P. J. B. Pereira

Synopsis: The enzyme glutamine synthetase from M. truncatula has been expressed, purified and crystallized. The crystals belonged to the monoclinic space group P2₁ and diffracted to 2.35 Å resolution.

Expression, purification, crystallization and preliminary X-ray analysis of maleylacetate reductase from Burkholderia sp. strain SJ98

A. Chauhan, Z. Islam, R. K. Jain and S. Karthikeyan

Synopsis: Purification and preliminary X-ray crystallographic analysis of maleylacetate reductase encoded by the pnpD gene is reported.

Purification, crystallization and preliminary crystallographic analysis of a thermostable endonuclease IV from Thermotoga maritima

R. C. Hughes, S. J. Tomanicek, J. D. Ng and L. Coates

Synopsis: The overexpression, purification and crystallization of endonuclease IV from T. maritima are reported. The crystals belonged to the hexagonal space group P6₁ and diffracted to 2.36 Å resolution.

Crystallization and preliminary X-ray analysis of NADH:rubredoxin oxidoreductase from Clostridium acetobutylicum

K. Nishikawa, Y. Shomura, S. Kawasaki, Y. Niimura and Y. Higuchi

Synopsis: NADH:rubredoxin oxidoreductase from C. acetobutylicum was expressed in E. coli, purified and crystallized. X-ray diffraction data were collected to a resolution of 2.1 Å.

Structure of glyceraldehyde-3-phosphate dehydrogenase from the archaeal hyperthermophile Methanocaldococcus jannaschii

A. D. Malay, Y. Bessho, M. J. Ellis, S. V. Antonyuk, R. W. Strange, S. S. Hasnain, A. Shinkai, B. Padmanabhan and S. Yokoyama

Structure of YqgQ protein from Bacillus subtilis, a conserved hypothetical protein

D. Lakshminarasimhan, S. Eswaramoorthy, S. K. Burley and S. Swaminathan

Synopsis: The crystal structure of the hypothetical protein YqgQ from B. subtilis is reported at 2.1 Å resolution.

The structure of BVU2987 from Bacteroides vulgatus reveals a superfamily of bacterial periplasmic proteins with possible inhibitory function

D. Das, R. D. Finn, D. Carlton, M. D. Miller, P. Abdubek, T. Astakhova, H. L. Axelrod, C. Bakolitsa, C. Chen, H.-J. Chiu, M. Chiu, T. Clayton, M. C. Deller, L. Duan, K. Ellrott, D. Ernst, C. L. Farr, J. Feuerhelm, J. C. Grant, A. Grzechnik, G. W. Han, L. Jaroszewski, K. K. Jin, H. E. Klock, M. W. Knuth, P. Kozbial, S. S. Krishna, A. Kumar, D. Marciano, D. McMullan, A. T. Morse, E. Nigoghossian, A. Nopakun, L. Okach, C. Puckett, R. Reyes, C. L. Rife, N. Sefcovic, H. J. Tien, C. B. Trame, H. van den Bedem, D. Weekes, T. Wooten, Q. Xu, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The crystal structure of the BVU2987 gene product from B. vulgatus (UniProt A6L4L1) reveals that members of the new Pfam family PF11396 (domain of unknown function; DUF2874) are similar to -lactamase inhibitor protein (BLIP) and YpmB.

Crystallization and preliminary X-ray crystallographic analysis of blood coagulation factor V-activating proteinase (RVV-V) from Russell's viper venom

D. Nakayama, Y. Ben Ammar and S. Takeda

Synopsis: The crystallization and preliminary X-ray crystallographic analysis of blood coagulation factor V-activating proteinase are reported. The best crystal diffracted to 1.9 Å resolution.

Structure of dihydrodipicolinate synthase from Methanocaldococcus jannaschii

B. Padmanabhan, R. W. Strange, S. V. Antonyuk, M. J. Ellis, S. S. Hasnain, H. Iino, Y. Agari, Y. Bessho and S. Yokoyama

Structure of a putative -phosphoglucomutase (TM1254) from Thermotoga maritima

R. W. Strange, S. V. Antonyuk, M. J. Ellis, Y. Bessho, S. Kuramitsu, A. Shinkai, S. Yokoyama and S. S. Hasnain

Purification, crystallization and initial X-ray diffraction study of human REV7 in complex with a REV3 fragment

K. Hara, T. Shimizu, S. Unzai, S. Akashi, M. Sato and H. Hashimoto

Synopsis: The crystallization and initial X-ray diffraction of REV7 in complex with REV3 is reported.

Crystallization and preliminary X-ray crystallographic analysis of a new crystal form of hydroxylamine oxidoreductase from Nitrosomonas europaea

P. E. Cedervall, A. B. Hooper and C. M. Wilmot

Synopsis: A new crystal form of N. europaea hydroxylamine oxidoreductase (space group P2₁2₁2) diffracted to 2.25 Å resolution at a third-generation synchrotron X-ray source.

Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Staphylococcus aureus

S. K. Hong, K. H. Kim and E. E. Kim

Synopsis: Malonyl-CoA:acyl-carrier protein transacylase (MCAT; FabD) from S. aureus has been cloned, overexpressed, purified and crystallized. The crystal belonged to space group P2₁, with unit-cell parameters a = 41.608, b = 86.717, c = 43.163 Å, = = 90, = 106.330°, and data were collected to 1.2 Å resolution using synchrotron radiation.

Crystallization and preliminary X-ray analysis of neoagarobiose hydrolase from Saccharophagus degradans 2-40

S. Lee, J. Y. Lee, S. C. Ha, J. Jung, D. H. Shin, K.-H. Kim and I.-G. Choi

Crystallization and preliminary X-ray analysis of a monomeric mutant of Azami-Green (mAG), an Aequorea victoria green fluorescent protein-like green-emitting fluorescent protein from the stony coral Galaxea fascicularis

T. Ebisawa, A. Yamamura, Y. Kameda, K. Hayakawa, K. Nagata and M. Tanokura

Synopsis: A monomeric mutant of Azami-Green from G. fascicularis was expressed, purified and crystallized using the sitting-drop vapour-diffusion method. The crystal belonged to space group P1 and diffracted X-rays to 2.20 Å resolution.

Preliminary crystallographic studies of purine nucleoside phosphorylase from the cariogenic pathogen Streptococcus mutans

Q.-M. Hou, X. Liu, E. Brostromer, L.-F. Li and X.-D. Su

Structure of D-lactate dehydrogenase from Aquifex aeolicus complexed with NAD⁺ and lactic acid (or pyruvate)

S. V. Antonyuk, R. W. Strange, M. J. Ellis, Y. Bessho, S. Kuramitsu, Y. Inoue, S. Yokoyama and S. S. Hasnain

The structure of an archaeal ribose-5-phosphate isomerase from Methanocaldococcus jannaschii (MJ1603)

R. W. Strange, S. V. Antonyuk, M. J. Ellis, Y. Bessho, S. Kuramitsu, S. Yokoyama and S. S. Hasnain

Crystallization and preliminary X-ray diffraction studies of FAD synthetase from Corynebacterium ammoniagenes

B. Herguedas, M. Martínez-Júlvez, S. Frago, M. Medina and J. A. Hermoso

Synopsis: Native and selenomethionine-labelled FAD synthetase from C. ammoniagenes have been crystallized by the hanging-drop vapour-diffusion method. A MAD data set for SeMet-labelled FAD synthetase was collected to 2.42 Å resolution, while data sets were collected to 1.95 Å resolution for the native crystals.

Role of tyrosine 131 in the active site of paAzoR1, an azoreductase with specificity for the inflammatory bowel disease prodrug balsalazide

C.-J. Wang, N. Laurieri, A. Abuhammad, E. Lowe, I. Westwood, A. Ryan and E. Sim

Synopsis: Tyr131 plays an important role in determining the substrate specificity and thermal stability of azoreductase 1 from P. aeruginosa (pAzoR1). The structure shows that the substrate methyl red binds deeper in the active site of the mutant, which is reflected in the change in enzymic properties.

The taming of small heat-shock proteins: crystallization of the -crystallin domain from human Hsp27

E. V. Baranova, S. Beelen, N. B. Gusev and S. V. Strelkov

Purification, crystallization and preliminary X-ray analysis of the PCNA2-PCNA3 complex from Sulfolobus tokodaii strain 7

A. Kawai, S. Higuchi, M. Tsunoda, K. T. Nakamura and S. Miyamoto

Synopsis: A PCNA2-PCNA3 complex which has recently been identified from S. tokodaii strain 7 was overexpressed, purified and crystallized in two crystal forms.

Crystallization and preliminary X-ray diffraction analysis of the MIF4G domain of DAP5

F. Frank, G. Virgili, N. Sonenberg and B. Nagar

Synopsis: The MIF4G domain of DAP5 was crystallized in two distinct crystal forms. Diffraction patterns have been analyzed and preliminary analysis, including molecular replacement, is presented here.

Structure of hypothetical Mo-cofactor biosynthesis protein B (ST2315) from Sulfolobus tokodaii

S. V. Antonyuk, R. W. Strange, M. J. Ellis, Y. Bessho, S. Kuramitsu, A. Shinkai, S. Yokoyama and S. S. Hasnain

Structure of SurE protein from Aquifex aeolicus VF5 at 1.5 Å resolution

S. V. Antonyuk, M. J. Ellis, R. W. Strange, Y. Bessho, S. Kuramitsu, A. Shinkai, S. Yokoyama and S. S. Hasnain

Crystallization of selenomethionyl exo--1,3-galactanase from the basidiomycete Phanerochaete chrysosporium

T. Ishida, Z. Fujimoto, H. Ichinose, K. Igarashi, S. Kaneko and M. Samejima

Synopsis: Selenomethionyl exo--1,3-galactanase from P. chrysosporium K-3 produced in Pichia pastoris was crystallized. The crystals diffracted to a resolution of 1.8 Å and belonged to space group P2₁.

Purification, crystallization and preliminary X-ray analysis of a deletion mutant of a major buckwheat allergen

Y. Kezuka, T. Itagaki, R. Satoh, R. Teshima and T. Nonaka

Synopsis: A 16 kDa buckwheat protein (BWp16) is a major allergen responsible for immediate hypersensitivity reactions including anaphylaxis. An immunologically active mutant of BWp16 was prepared and a three-wavelength MAD data set was collected from a crystal of selenomethionine-labelled mutant protein.

Structure of the first PDZ domain of human PSD-93

M. Fiorentini, A. K. Nielsen, O. Kristensen, J. S. Kastrup and M. Gajhede

Expression, crystallization and preliminary X-ray diffraction analysis of a modification subunit of a putative type I restriction enzyme from Vibrio vulnificus YJ016

H.-J. Lee, K. Nishi, J.-M. Song and J.-S. Kim

Synopsis: The crystallization of an HsdM subunit, a component of the methyltransferase of a putative type I restriction enzyme, from V. vulnificus YJ016 and the collection of diffraction data to 1.86 Å resolution are reported.

The high-resolution structure of the extracellular domain of human CD69 using a novel polymer

P. Kolenko, T. Skálová, O. Vanek, A. Stepánková, J. Dusková, J. Hasek, K. Bezouska and J. Dohnálek

Synopsis: The structure of the extracellular domain of human CD69 was crystallized using a novel polymer precipitant: di[poly(ethylene glycol)] adipate. The structure was refined at 1.37 Å resolution.

A preliminary X-ray study of D,D-heptose-1,7-bisphosphate phosphatase from Burkholderia thailandensis E264

M.-S. Kim and D. H. Shin

The open and closed conformations of two SpoIIAA-like proteins (YP_749275.1 and YP_001095227.1) provide insights into membrane association and ligand binding

A. Kumar, A. Lomize, K. K. Jin, D. Carlton, M. D. Miller, L. Jaroszewski, P. Abdubek, T. Astakhova, H. L. Axelrod, H.-J. Chiu, T. Clayton, D. Das, M. C. Deller, L. Duan, J. Feuerhelm, J. C. Grant, A. Grzechnik, G. W. Han, H. E. Klock, M. W. Knuth, P. Kozbial, S. S. Krishna, D. Marciano, D. McMullan, A. T. Morse, E. Nigoghossian, L. Okach, R. Reyes, C. L. Rife, N. Sefcovic, H. J. Tien, C. B. Trame, H. van den Bedem, D. Weekes, Q. Xu, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: The determination of the crystal structures of two orthologous proteins from different Shewanella species has uncovered a resemblance to CRAL-TRIO carrier proteins, which suggests that they function as transporters of small nonpolar molecules. One protein adopts an open conformation, while the other adopts a closed structure that may act as a conformational switch in the transport of ligands at the membrane surface.

Cloning, recombinant production, crystallization and preliminary X-ray diffraction analysis of SDF2-like protein from Arabidopsis thaliana

J. Radzimanowski, S. Ravaud, A. Schott, S. Strahl and I. Sinning

Crystallization and preliminary X-ray diffraction studies of the carbohydrate-recognition domain of SIGN-R1, a receptor for microbial polysaccharides and sialylated antibody on splenic marginal zone macrophages

N. Silva-Martin, J. D. Schauer, C. G. Park and J. A. Hermoso

Synopsis: The carbohydrate-recognition domain of the SIGN-R1 receptor from M. musculus has been crystallized by the hanging-drop vapour-diffusion method. A native data set has been collected to 1.87 Å resolution.

Structure of human glycolate oxidase in complex with the inhibitor 4-carboxy-5-[(4-chlorophenyl)sulfanyl]-1,2,3-thiadiazole

J.-M. Bourhis, C. Vignaud, N. Pietrancosta, F. Guéritte, D. Guénard, F. Lederer and Y. Lindqvist

Preliminary crystallographic analysis of mouse Elf3 C-terminal DNA-binding domain in complex with type II TGF- receptor promoter DNA

V. B. Agarkar, N. D. Babayeva, A. Rizzino and T. H. Tahirov

Synopsis: The cloning, expression, purification and crystallization of the mouse Elf3 C-terminal DNA-binding domain in complex with mouse type II TGF- receptor promoter DNA are reported. The crystals were characterized and an X-ray diffraction data set was collected to a resolution of 2.2 Å.

Structure of the first representative of Pfam family PF09410 (DUF2006) reveals a structural signature of the calycin superfamily that suggests a role in lipid metabolism

H.-J. Chiu, C. Bakolitsa, A. Skerra, A. Lomize, D. Carlton, M. D. Miller, S. S. Krishna, P. Abdubek, T. Astakhova, H. L. Axelrod, T. Clayton, M. C. Deller, L. Duan, J. Feuerhelm, J. C. Grant, S. K. Grzechnik, G. W. Han, L. Jaroszewski, K. K. Jin, H. E. Klock, M. W. Knuth, P. Kozbial, A. Kumar, D. Marciano, D. McMullan, A. T. Morse, E. Nigoghossian, L. Okach, J. Paulsen, R. Reyes, C. L. Rife, H. van den Bedem, D. Weekes, Q. Xu, K. O. Hodgson, J. Wooley, M.-A. Elsliger, A. M. Deacon, A. Godzik, S. A. Lesley and I. A. Wilson

Synopsis: NE1406, the first structural representative of PF09410, reveals a lipocalin-like fold with features that suggest involvement in lipid metabolism. In addition, NE1406 provides potential structural templates for two other protein families (PF07143 and PF08622).

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the collagen-binding region of RspB from Erysipelothrix rhusiopathiae

A. S. Devi, Y. Ogawa, Y. Shimoji and K. Ponnuraj

Synopsis: The expression, purification and crystallization of the collagen-binding region of the E. rhusiopathiae surface protein RspB is described. The crystals diffracted to 2.2 Å resolution using synchrotron radiation.

Crystallization and X-ray structure of cold-shock protein E from Salmonella typhimurium

H. P. Morgan, M. A. Wear, I. McNae, M. P. Gallagher and M. D. Walkinshaw

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