forthcoming articles in Acta Crystallographica Section F

The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.

This list will generally be short, as papers in this journal are published online as soon as proofs are returned.

See also Forthcoming articles in all IUCr journals.


Accepted 18 December 2014

Structure of the apo anti-influenza CH65 Fab

P. S. Lee, A. J. Arnell and I. A. Wilson


Accepted 18 December 2014

Expression, crystallization and preliminary crystallographic data analysis of VioD, a hydroxylase in the violacein-biosynthesis pathway

T. Ran, M. Gao, Q. Wei, J. He, L. Tang, W. Wang and D. Xu

Synopsis: VioD, a hydroxylase in violacein biosynthesis pathway, was crystallized and a complete 1.7 Å dataset was collected. The crystal belongs to space group P31 with unit-cell parameters a = b = 90.0, c = 94.5 Å, [alpha] = [beta] = 90, [gamma] = 120 o.


Accepted 17 December 2014

Preliminary X-ray analysis of the binding domain of the soybean vacuolar sorting receptor with a sorting determinant of seed storage protein

N. Maruyama, T. Goshi, S. Sugiyama, M. Niiyama, H. Adachi, K. Takano, S. Murakami, T. Inoue, Y. Mori, H. Matsumura and B. Mikami

Synopsis: Preliminary structural analysis of VSR in complex with the C-terminal sorting determinant of seed storage proteins


Accepted 17 December 2014

Crystallization and preliminary X-ray diffraction analysis of the N-terminal domain from Paenibacillus barcinonensis Xylanase 10C, containing the tandem CBM22-1-CBM22-2

M. Ángela Sainz-Polo, B. González, F. J. Pastor and J. Sanz-Aparicio

Synopsis: The Paenibacillus barcinonensis Xylanase 10C N-terminal domain, containing the tandem CBM22-1-CBM22-2, was overexpressed, purified and crystallized, and its preliminary X-ray diffraction analysis is reported at 2.43 Å resolution.


Accepted 15 December 2014

The three-dimensional structure of the cellobio­hydrolase Cel7A from Aspergillus fumigatus at 1.5 Å resolution

O. V. Moroz, M. Maranta, T. Shaghasi, P. V. Harris, K. S. Wilson and G. J. Davies

Synopsis: The structure of an industrially important thermally stable cellulase, A. fumigatus cellobiohydrolase I, has been solved in apo and product-complex forms at 1.8 and 1.5 Å resolution, respectively.


Accepted 15 December 2014

Recombinant expression, purification, crystallization and preliminary X-ray diffraction analysis of Haemophilus influenzae BamD and BamCD complex

J. Lei, X. Cai, X. Ma, L. Zhang, Y. Li, X. Dong, J. St Geme and G. Meng


Accepted 15 December 2014

Cloning, expression, refolding, purification and preliminary crystallographic analysis of the sensory domain of the Campylobacter chemoreceptor for aspartate A (CcaA)

M. A. Machuca, Y. C. Liu and A. Roujeinikova

Synopsis: A periplasmic sensory domain of the C. jejuni chemoreceptor for aspartate A (CcaA) has been purified and crystallized by the hanging-drop vapour-diffusion method. A diffraction data set has been collected to 1.4 Å resolution.


Accepted 11 December 2014

Systematic analysis of protein-detergent complexes applying dynamic light scattering to optimize solutions for crystallization trials

A. Meyer, K. Dierks, R. Hussein, K. Brillet, H. Brognaro and C. Betzel

Synopsis: Application of in situ dynamic light scattering to solutions of protein-detergent complexes permits characterization of these complexes in samples as small as 2 µl in volume.


Accepted 11 December 2014

Purification, crystallization and preliminary X-ray diffraction analysis of SpaD, a backbone-pilin subunit encoded by the fimbrial spaFED operon in Lactobacillus rhamnosus GG

P. Chaurasia, I. von Ossowski, A. Palva and V. Krishnan

Synopsis: SpaD, a backbone-pilin fragment from L. rhamnosus GG, was crystallized and X-ray diffraction data were collected to a resolution of 2.0 Å. In-house iodide-derivative data were collected to 2.2 Å resolution for phase determination by the single-wavelength anomalous dispersion method.


Accepted 11 December 2014

Purification, crystallization and preliminary X-ray crystallographic studies of Rv3899c from Mycobacterium tuberculosis

Y. Song, J. Liu, D.-F. Li, H. Li, S. Wang, D.-C. Wang, J. Zhou and L. Bi

Synopsis: The cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of Rv3899c from M. tuberculosis are described.


Accepted 5 December 2014

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes

M. Caffrey

Synopsis: A comprehensive and up-to-date review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes is reported. Recent applications of the method for in situ serial crystallography at X-ray free-electron lasers and synchrotrons are described.


Accepted 5 December 2014

Structures of the N-acetyltransferase domain of Xylella fastidiosa N-acetyl-L-glutamate synthase/kinase with and without a His tag bound to N-acetyl-L-glutamate

G. Zhao, Z. Jin, N. M. Allewell, M. Tuchman and D. Shi

Synopsis: Structures of the catalytic N-acetyltransferase (NAT) domain of the bifunctional N-acetyl-L-glutamate synthase/kinase (NAGS/K) from Xylella fastidiosa bound to N-acetyl-L-glutamate (NAG) with and without an N-terminal His tag have been solved and refined at 1.7 and 1.4 Å resolution, respectively.


Accepted 2 December 2014

Crystallization and preliminary X-ray analysis of the NAD+-reducing [NiFe] hydrogenase from Hydrogenophilus thermoluteolus TH-1

M. Taketa, H. Nakagawa, M. Habukawa, H. Osuka, K. Kihira, H. Komori, N. Shibata, M. Ishii, Y. Igarashi, H. Nishihara, K.-S. Yoon, S. Ogo, Y. Shomura and Y. Higuchi

Synopsis: An NAD+-reducing [NiFe] hydrogenase was purified and crystallized. An X-ray diffraction data set was collected to a resolution of 2.58 Å.


Accepted 2 December 2014

Three-dimensional structure of a variant `Termamyl-like' Geobacillus stearothermophilus [alpha]-amylase at 1.9 Å resolution

W. A. Offen, A. Viksoe-Nielsen, T. V. Borchert, K. S. Wilson and G. J. Davies

Synopsis: The three-dimensional single-crystal X-ray structure of a thermally stable and industrially relevant bacterial [alpha]-amylase variant has been determined at 1.9 Å resolution.


Accepted 28 November 2014

Crystallization and preliminary X-ray crystallographic analysis of the CARD domain of apoptosis repressor with CARD (ARC)

S. H. Kim and H. H. Park

Synopsis: The CARD domain of apoptosis repressor with CARD (ARC) was purified and crystallized. Crystals were obtained at 293 K and diffracted to a resolution of 2.1 Å; they belonged to space group P61 or P65, with unit-cell parameters a = 98.28, b = 98.28, c = 51.86 Å, [alpha] = 90, [beta] = 90, [gamma] = 120°.


Accepted 28 November 2014

Structures of Drosophila melanogaster Rab2 and Rab3 bound to GMPPNP

J. A. Lardong, J. H. Driller, H. Depner, C. Weise, A. Petzoldt, M. C. Wahl, S. J. Sigrist and B. Loll

Synopsis: Crystal structures of Rab2 and Rab3 from D. melanogaster in complex with GMPPNP are presented.


Accepted 27 November 2014

Expression, purification and crystallization of a membrane-associated, catalytically active type I signal peptidase from Staphylococcus aureus

Y. T. Ting, G. Batot, E. N. Baker and P. G. Young

Synopsis: The type I signal peptidase from S. aureus Newman strain was crystallized as a fusion protein with maltose-binding protein. Preliminary X-ray data showed that the crystals diffracted to 2.05 Å resolution.


Accepted 27 November 2014

Cleaning protocols for crystallization robots: preventing protease contamination

A. Naschberger, B. G. Fürnrohr, T. Dunzendorfer-Matt, C. A. Bonagura, D. Wright, K. Scheffzek and B. Rupp

Synopsis: A protease in the common commercial enzyme cleaner Zymit used for the cleaning of crystallization robots cannot be completely inactivated by EDTA.


Accepted 26 November 2014

Overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of SF173 from Shigella flexneri

H.-N. Kim, J.-G. An, Y.-S. Lee, S.-H. Seok, H.-S. Yoo and M.-D. Seo

Synopsis: This article describes the preliminary crystallographic data of SF173 from Shigella flexneri.


Accepted 26 November 2014

Crystallization and preliminary X-ray analysis of the type IV restriction endonuclease ScoMcrA from Streptomyces coelicolor, which cleaves both Dcm-methylated DNA and phosphoro­thioated DNA

G. Liu, Z. Zhang, G. Zhao, Z. Deng, G. Wu and X. He

Synopsis: The type IV restriction endonuclease ScoMcrA, which cleaves both Dcm-methylated DNA and phosphorothioated DNA, was crystallized and the crystals diffracted to 3.35 Å resolution. The space group and the unit-cell parameters were determined.


Accepted 24 November 2014

Purification, crystallization and preliminary crystallographic analysis of DR0248, an MNT-HEPN fused protein from Deinococcus radiodurans

G. Pesce, S. Pellegrino, S. McSweeney, A. Goncalves and D. de Sanctis

Synopsis: DR0248, a MNT-HEPN fused protein from D. radiodurans, was expressed, purified and crystallized in two crystal forms. MAD phases were obtained from zinc identified in the protein by X-ray fluorescence.


Accepted 23 November 2014

Crystallization and preliminary X-ray analysis of the C-terminal fragment of PorM, a subunit of the Porphyromonas gingivalis type IX secretion system

J. Stathopulos, C. Cambillau, E. Cascales, A. Roussel and P. Leone

Synopsis: Crystals of the C-terminal part of PorM obtained by limited proteolysis of the purified recombinant protein and grown from PEG solutions were tetragonal (space group P43212) and diffracted to 2.85 Å resolution.


Accepted 22 November 2014

Crystallization and preliminary crystallographic studies of a novel noncatalytic carbohydrate-binding module from the Ruminococcus flavefaciens cellulosome

I. Venditto, A. Goyal, A. Thompson, L. M. A. Ferreira, C. M. G. A. Fontes and S. Najmudin

Synopsis: A novel carbohydrate-binding module (CBM-Rf6A) of a modular family 9 glycoside hydrolase from R. flavefaciens was purified and crystallized, and data were collected for the native protein and a selenomethionine derivative to 1.75 and 1.5 Å resolution, respectively. The crystals belonged to orthorhombic and cubic space groups, respectively, and the structure was solved by a single-wavelength anomalous dispersion experiment.


Accepted 22 November 2014

Zinc-substituted pseudoazurin solved by S/Zn-SAD phasing

R. Gessmann, M. Papadovasilaki, E. Drougkas and K. Petratos

Synopsis: The Cu2+ ion of the blue copper protein pseudoazurin from A. faecalis was replaced by a Zn2+ ion and the resulting protein was phased by SAD using the anomalous signal of the S and Zn atoms. The Zn2+ ion lies closer to the plane of its strong ligand atoms (His40 N[delta]1, Cys78 S[gamma] and His81 N[delta]1) and attracts its axial ligand Met86 S[delta] closer.


Accepted 20 November 2014

Expression and crystallization of a bacterial glycoside hydrolase family 116 [beta]-glucosidase from Thermoanaerobacterium xylanolyticum

S. Sansenya, R. Mutoh, R. Charoenwattanasatien, G. Kurisu and J. R. Ketudat Cairns

Synopsis: A thermostable glycoside hydrolase family 116 [beta]-glucosidase from T. xylanolyticum was expressed in E. coli, purified and crystallized. The crystal diffracted to 3.15 Å resolution with P21 symmetry.


Accepted 18 November 2014

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the histone-like HU protein from Spiroplasma melliferum KC3

K. Boyko, M. Gorbacheva, T. Rakitina, D. Korzhenevskiy, A. Vanyushkina, D. Kamashev, A. Lipkin and V. Popov

Synopsis: The HU protein from the parasitic mycoplasma S. melliferum KC3 was cloned, overexpressed in E. coli and purified. Crystals of the protein that diffracted to 1.36 Å resolution were obtained for the first time.


Accepted 18 November 2014

Protein purification, crystallization and preliminary X-ray diffraction analysis of L-arabinose isomerase from Lactobacillus fermentum CGMCC2921

Z. Xu, S. Li, J. Liang, X. Feng and H. Xu

Synopsis: L-Arabinose isomerase from L. fermentum CGMCC2921 was heterologously expressed, purified and crystallized, and preliminary X-ray diffraction analysis was successfully carried out.




































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