forthcoming articles in Acta Crystallographica Section F

The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.

This list will generally be short, as papers in this journal are published online as soon as proofs are returned.

See also Forthcoming articles in all IUCr journals.


Accepted 27 February 2015

Expression, purification, crystallization and preliminary crystallographic analysis of a GH-20 [beta]-N-acetylglucosaminidase from the marine bacterium Vibrio harveyi

P. Meekrathok, M. Bürger, A. T. Porfetye, I. R. Vetter and W. Suginta

Synopsis: Vibrio harveyi [beta]-N-acetylglucosaminidase (VhGlcNAcase) is a glycoside hydrolase that degrades chitooligosacharides, yielding N-acetylglucosamine (GlcNAc) as the final product. Single crystals of VhGlcNAcase wild-type and D437A mutant were grown and diffracted to highest resolutions of 2.43 and 2.60 Å, respectively.


Accepted 26 February 2015

Crystallization and preliminary X-ray characterization of the eukaryotic replication terminator Reb1-Ter DNA complex

R. Jaiswal, S. K. Singh, D. Bastia and C. R. Escalante

Synopsis: This crystallization and data collection for a complex of Reb1 bound to Ter3 DNA are reported.


Accepted 26 February 2015

Structure of the omalizumab Fab

R. K. Jensen, M. Plum, L. Tjerrild, T. Jakob, E. Spillner and G. R. Andersen

Synopsis: The therapeutic antibody omalizumab inhibits binding of IgE to its receptors. Two crystal structures of the omalizumab Fab fragment have been determined at 1.9 and 3.0 Å resolution, respectively.


Accepted 25 February 2015

Crystallization of mutants of the Turnip yellow mosaic virus protease/ubiquitin hydrolase designed to prevent protease self-recognition

M. Ayach and S. Bressanelli

Synopsis: Mutants of Turnip yellow mosaic virus protease/ubiquitin hydrolase designed to prevent self-recognition were produced and crystallized as monomers.


Accepted 25 February 2015

Towards a molecular understanding of the apicomplexan actin motor - on a road to novel targets for malaria remedies?

E.-P. Kumpula and I. Kursula

Synopsis: In this review, our current structural understanding of the apicomplexan glideosome and actin regulation is described.


Accepted 25 February 2015

Structural basis of the substrate specificity of FPOD/FAOD family revealed by fructosyl peptide oxidase from Eupenicillium terrenum

W. Gan, F. Gao, K. Xing, M. Jia, H. Liu and W. Gong

Synopsis: The crystal structure of fructosyl peptide oxidase from Eupenicillium terrenum (EtFPOX) was determined at 1.9 Å resolution. This is the first structure of group I FPOD that prefers [alpha]-fructosyl peptide substrates.


Accepted 25 February 2015

Cloning, expression, crystallization and preliminary X-ray studies of a superfolder GFP fusion of cyanobacterial Psb32

P. Liauw, D. Kannchen, R. Gasper, N. Dyczmons-Nowaczyk, M. M. Nowaczyk and E. Hofmann

Synopsis: Psb32 from T. elongatus was expressed using a novel vector library for the generation of superfolder fluorescent fusions proteins. X-ray diffraction data were collected to a resolution of 2.3 Å from a crystal belonging to space group P6122.


Accepted 24 February 2015

The X-ray structure of human P-cadherin EC1-EC2 in a closed conformation provides insights into the type-I cadherin dimerization pathway

A. Dalle Vedove, A. P. Lucarelli, V. Nardone, A. Matino and E. Parisini

Synopsis: The crystal structure of human P-cadherin EC1-EC2 in closed conformation is reported at 1.62 Å resolution, showing a Trp2 double conformation in the binding pocket and a novel monomeric packing arrangement.


Accepted 21 February 2015

Crystallization and preliminary X-ray diffraction analysis of interactions of Aeromonas hydrophila MtaN-1 with S-adenosylhomocysteine

Y. Xu, C.-S. Quan, X. Jin, X. Jin, J. Zhao, L. Jin, J.-S. Kim, J. Guo, S. Fan and N.-C. Ha


Accepted 21 February 2015

Crystallization and preliminary X-ray diffraction analysis of an endo-1,4-[beta]-D-glucanase from Aspergillus aculeatus F-50

Y. Chen, J.-W. Huang, C.-C. Chen, H.-L. Lai, J. Jin and R.-T. Guo

Synopsis: A GH12 endoglucanase from A. aculeatus F-50 was expressed in P. pastoris, crystallized and diffracted to a high resolution of 1.6 Å.


Accepted 21 February 2015

Purification, crystallization and preliminary X-ray analysis of the periplasmic haem-binding protein HutB from Vibrio cholerae

S. Agarwal, M. Biswas and J. Dasgupta

Synopsis: HutB from V. cholerae has been cloned, overexpressed, purified and crystallized in monomeric form. Crystals belonging to the space group P43212 produced diffraction data to 2.4 Å resolution.


Accepted 20 February 2015

Crystallization and preliminary X-ray crystallographic analysis of the sclerostin-neutralizing Fab AbD09097

V. Boschert, E.-M. Muth, A. Knappik, C. Frisch and T. D. Mueller

Synopsis: A neutralizing Fab antibody fragment directed against the Wnt antagonist sclerostin was crystallized and its structure has been determined by molecular replacement.


Accepted 12 February 2015

Identification and structure solution of fragment hits against kinetoplastid N-myristoyltransferase

D. A. Robinson and P. G. Wyatt

Synopsis: N-myristoyltransferase (NMT) has been shown to be an attractive target for the development of novel therapeutic agents for the treatment of human African trypanosomiasis (HAT). A fragment library has been screened using NMR spectroscopy and the binding mode of hits confirmed by X-ray crystallography using Leishmania major NMT (LmNMT) as a structural surrogate.


Accepted 9 February 2015

The structure of hookworm platelet inhibitor (HPI), a CAP superfamily member from Ancylostoma caninum

D. Ma, I. M. B. Francischetti, J. M. C. Ribeiro and J. F. Andersen

Synopsis: Hookworm platelet inhibitor belongs to the cysteine-rich/antigen-5/pathogenesis-related-1 (CAP) protein family. It has been linked with integrin-antagonist, antiplatelet activity found in extracts of Ancylostoma caninum hookworms.


Accepted 5 February 2015

Production, purification and crystallization of a trans-sialidase from Trypanosoma vivax

C. L. F. Haynes, P. Ameloot, H. Remaut, N. Callewaert, Y.G.-J. Sterckx and S. Magez

Synopsis: The production, purification, and crystallization of a recently identified trans-sialidase from Trypanosoma vivax is described. The purified enzyme is active and produces diffraction-quality crystals using the sitting drop vapour diffusion method. The crystals belong to space group P212121 and diffract to 2.5 Å.


Accepted 4 February 2015

Structure of a histidine triad family protein from Entamoeba histolytica, bound to sulfate, AMP and GMP

D. D. Lorimer, R. Choi, A. Abramov, S. Nakazawa-Hewitt, A. S. Gardberg, W. C. Van Voorhis, B. L. Staker, P. J. Myler and T. E. Edwards

Synopsis: Structures of the histidine triad family protein from Entamoeba histolytica were solved in complex with sulfate, AMP or GMP. The three structures show very similar nucleotide poses with the sulfate occupying the same space as the alpha phosphate of the nucleotides.


Accepted 27 January 2015

Structure of uridine diphosphate N-acetylglucosamine pyro­phosphorylase from Entamoeba histolytica

T. E. Edwards, A. S. Gardberg, I. Q. H. Phan, Y. Zhang, B. L. Staker, P. J. Myler and D. D. Lorimer

Synopsis: Uridine diphosphate N-acetylglucosamine pyrophosphorylase (UAP) synthesizes UDP-GlcNAc. Entamoeba histolytica UAP appears to lack an N-terminal extension partially involved in allosteric regulation by small molecule inhibitors of UAP from other parasites.


Accepted 27 January 2015

Structures of aspartate aminotransferases from Trypanosoma brucei, Leishmania major and Giardia lamblia

J. Abendroth, R. Choi, A. Wall, M. C. Clifton, C. M. Lukacs, B. L. Staker, W. van Voorhis, P. Myler, D. D. Lorimer and T. E. Edwards

Synopsis: Structures of aspartate aminotransferases from eukaryotic pathogens were solved in complex with pyridoxal phosphate. The four structures show either the open or the closed conformation of the enzyme. The structures highlight the challenges for structure based design of inhibitors targeting the active site, while showing options for inhibitor design targeting the N-terminal arm.


Accepted 16 January 2015

The structure of tubulin-binding cofactor A from Leishmania major infers a mode of association during the early stages of microtubule assembly

K. L. Barrack, P. K. Fyfe and W. N. Hunter

Synopsis: The structure of a tubulin-binding co-factor from Leishmania major is reported and compared with yeast, plant and human orthologues.


Accepted 16 January 2015

X-ray structure of Plasmodium falciparum di­hydroorotate de­hydrogenase bound to a potent and selective N-phenyl-benzamide inhibitor reveals novel binding-site interactions

X. Deng, D. Matthews, P. K. Rathod and M. A. Phillips

Synopsis: The X-ray structure of the antimalarial drug target dihydroorotate dehydrogenase bound to a potent and selective benzamide based inhibitor shows novel binding site interactions.


Accepted 15 January 2015

Recombinant production, crystallization and crystal structure determination of di­hydroorotate de­hydrogenase from Leishmania viannia braziliensis

R. Almeida Garcia Reis, E. Lorenzato, V. C. Silva and M. C. Nonato

Synopsis: The crystal structure of Leishmania braziliensis dihydroorotate dehydrogenase, a potential target for drug development against cutaneous leishmaniasis, is reported.


Accepted 6 January 2015

Structure of Cryptosporidium IMP de­hydrogenase bound to an inhibitor with in vivo antiparasitic activity

Y. Kim, M. Makowska-Grzyska, S. K. Gorla, D. R. Gollapalli, G. D. Cuny, A. Joachimiak and L. Hedstrom

Synopsis: Compound P131 displays antiparasitic activity in a mouse model of Cryptosporidium infection, validating IMP dehydrogenase (IMPDH) as a drug target. Here we report the structure of the enzyme-substrate-P131 complex at 2.05 Å resolution. The structure is an important step to further refine the design of IMPDH inhibitors.


Accepted 29 December 2014

Structure of a CutA1 divalent-cation tolerance protein from Cryptosporidium parvum, the protozoal parasite responsible for cryptosporidiosis

G. W. Buchko, J. Abendroth, M. C. Clifton, H. Robinson, Y. Zhang, S. N. Hewitt, B. L. Staker, T. E. Edwards, W. C. Van Voorhis and P. J. Myler

Synopsis: C. parvum is the primary protozoan parasite from the Cryptosporidium genus responsible for cryptosporidiosis in humans. Here, the 2.0 Å resolution structure of the divalent-cation tolerance protein CutA1 encoded in the genome of C. parvum Iowa II is described.


Accepted 23 December 2014

Solution-state NMR structure of the putative morphogene protein BolA (PFE0790c) from Plasmodium falciparum

G. Buchko, A. Yee, A. Semesi, P. J. Myler, C. H. Arrowsmith and R. Hui

Synopsis: Parasitic protozoans of the Plasmodium type are responsible for malaria, a disease that kills approximately one child every minute in Africa and ~2000 people per day worldwide. Here, the solution structure of the 84-residue BolA protein PFE0790c from the deadliest Plasmodium species, P. falciparum, is reported.




































Copyright © International Union of Crystallography
IUCr Webmaster