The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology Communications.
Crystal structure of a thiolase from Escherichia coli at 1.8 Å resolution
Thiolases catalyze the Claisen condensation of two acetyl-CoA molecules to acetoacetyl-CoA as well as the reverse degradative reaction. The present research work revealed that various structural aspects of E. coli thiolase including enzyme kinetics, substrate binding, tetrameric organization and active-site water network.
Crystal structure of the cyan fluorescent protein Cerulean-S175G
The crystal structure of Cerulean-S175G was compared with those of Cerulean and SCFP3A, which are notable variants of enhanced cyan fluorescent protein (ECFP). A detailed comparison of the three structures revealed that the notable conformational changes of ECFP variants can be understood mainly in terms of the interaction between the Trp66 residue of the chromophore and residues 145–148 of β-strand 7.
Structural insights into the catalytic reaction trigger and inhibition of D-3-hydroxybutyrate dehydrogenase
Crystal structures of A. faecalis D-3-hydroxybutyrate dehydrogenase in complex with NAD+ and D-3-hydroxybutyrate, malonate or methylmalonate reveal the mechanisms of the catalytic reaction and its inhibition.