forthcoming articles in Acta Crystallographica Section F

The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.

This list will generally be short, as papers in this journal are published online as soon as proofs are returned.

See also Forthcoming articles in all IUCr journals.


Accepted 16 September 2014

Crystallization and preliminary X-ray crystallographic analysis of an artificial molten-globular-like triosephosphate isomerase protein of mixed phylogenetic origin

V. D. Goyal, P. Yadav, A. Kumar, B. Ghosh and R. D. Makde

Synopsis: The protein ccTIM is an engineered variant of the triosephosphate isomerase (TIM) enzyme and has sequence features from both prokaryotic and eukaryotic members of the TIM protein family. Moreover, it is as active as a native protein despite sharing some biophysical characteristics with a molten globular variant. This inscrutable protein was crystallized using the microbatch method and a full X-ray diffraction data set was collected to 2.2 Å resolution using an Indian synchrotron-radiation source.


Accepted 15 September 2014

New crystal structures of adenylate kinase from Streptococcus pneumonia D39 in two conformations

T. T. Thach and S. Lee


Accepted 10 September 2014

Purification, crystallization, and preliminary crystallographic investigation of FrnE, a disulfide oxidoreductase from Deinococcus radiodurans

L. Panicker, H. S. Misra and S. C. Bihani

Synopsis: drFrnE, a disulfide oxidoreductase from Deinococcus radiodurans was crystallized and preliminary diffraction data obtained. Crystal belonged to space group P21221 and diffracted to 1.8 Å resolution.


Accepted 9 September 2014

Expression, purification, crystallization, and preliminary X-ray crystallographic analysis of the extracellular olfactomedin domain from gliomedin

H. Han and P. Kursula

Synopsis: Gliomedin has an extracellular domain, which mediates interactions between the myelinating Schwann cell and the axonal surface and plays a role in the development and maintenance of the nodes of Ranvier. We report here the purification and crystallization of this olfactomedin domain.


Accepted 8 September 2014

Structure of Type II Dehydroquinase from Pseudomonas aeruginosa

S. Reiling, A. Kelleher, M. M. Matsumoto, G. Robinson and O. A. Asojo

Synopsis: The recombinant production and structural analysis of catalytically active Type II dehydroquinase from P. aeruginosa is presented.


Accepted 5 September 2014

Structure of an Escherichia coli Hfq-RNA complex at 0.97 Å resolution

E. C. Schulz and O. Barabas

Synopsis: The crystal structure of the RNA chaperone, Hfq in complex with RNA is presented at 0.97 Å resolution.


Accepted 5 September 2014

In situ proteolysis, crystallization and preliminary X-ray diffraction analysis of a VHH that binds listeria internalin B

I. Huh, R. Gene, J. Kumaran, C. R. MacKenzie and C. L. Brooks

Synopsis: VHH R303 binds the Listeria virulence factor InlB. Here, it is reported that crystals of R303 were obtained following in situ proteolysis with trypsin. Trypsin removed the flexible tag region of the protein, creating a homogenous protein preparation. The crystals diffracted to 1.3 Å resolution.


Accepted 4 September 2014

Purification, crystallization and preliminary X-ray crystallographic analysis of the flagellar accessory protein FlaH from the methanogenic archaeon Methanocaldococcus jannaschii

V. A. Meshcheryakov, Y.-H. Yoon, H. Matsunami and M. Wolf

Synopsis: The flagellar accessory protein FlaH from M. jannaschii has been purified and crystallized. The crystal structure has been solved using the MAD method.


Accepted 3 September 2014

Purification, crystallization, preliminary X-ray diffraction and molecular-replacement studies of great cormorant (Phalacrocorax carbo) haemoglobin

G. Jagadeesan, P. Malathy, K. Gunasekaran, S. Harikrishna Etti and S. Aravindhan


Accepted 2 September 2014

Crystallization and preliminary X-ray diffraction data of [beta]-galactosidase from Aspergillus niger

A. Rico-Díaz, Á. Vizoso Vázquez, M. E. Cerdán, M. Becerra and J. Sanz-Aparicio

Synopsis: [beta]-Galactosidase from A. niger has been expressed in S. cerevisiae, purified and crystallized in its deglycosylated form. X-ray data have been collected to 1.8 Å resolution.


Accepted 2 September 2014

Structure of a short-chain dehydrogenase/reductase (SDR) within a genomic island from a clinical strain of Acinetobacter baumannii

B. S. Shah, S. G. Tetu, S. J. Harrop, I. T. Paulsen and B. C. Mabbutt

Synopsis: The structure of a short-chain dehydrogenase encoded within genomic islands of A. baumannii strains has been solved to 2.4 Å resolution. This classical SDR incorporates a flexible helical subdomain. The NADP-binding site and catalytic side chains are identified.


Accepted 2 September 2014

Purification, Crystallization and Preliminary X-ray Diffraction Analysis of Pyridoxal Kinase from Plasmodium falciparum (PfPdxK)

T. Kronenberger, S. Lunev, C. Wrenger and M. R. Groves

Synopsis: The cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of PdxK from P. falciparum are described.


Accepted 1 September 2014

Crystallization and preliminary X-ray diffraction studies of the C-terminal domain of Chlamydia trachomatis CdsD

G. Meriläinen and R. K. Wierenga

Synopsis: The C-terminal domain of C. trachomatis CdsD was purified and crystallized. The preliminary X-ray diffraction studies are presented.


Accepted 31 August 2014

Optimization of crystallization conditions for biological macromolecules

A. McPherson and B. Cudney

Synopsis: Strategies and procedures are surveyed for the optimization of macromolecular crystal growth conditions. The objective of optimization is to grow crystals of the greatest degree of perfection and that yield the most accurate and precise X-ray diffraction data.


Accepted 28 August 2014

Crystallization and preliminary X-ray diffraction analysis of full-length spr1814, a response regulator of Streptococcus pneumoniae, in complex with a phosphoryl analogue

A. Park, J. S. Oh, Y. M. Chi and S. W. Jeong

Synopsis: A crystal of the full-length response regulator spr1814 of S. pneumoniae in complex with a phosphoryl analogue was obtained and diffraction data were collected to 2.0[1.9?] Å resolution.


Accepted 28 August 2014

Structure of Streptococcus agalactiae glyceraldehyde-3-phosphate dehydrogenase holoenzyme reveals a novel surface

C. A. Ayres, N. Schormann, O. Senkovich, A. Fry, S. Banerjee, G. C. Ulett and D. Chattopadhyay


Accepted 27 August 2014

Production, crystallization and preliminary crystallographic analysis of Allochromatium vinosum thiosulfate dehydrogenase TsdA, an unusual acidophilic c-type cytochrome

J. A. Brito, A. Gutierres, K. Denkmann, C. Dahl and M. Archer

Synopsis: The thiosulfate dehydrogenase TsdA from A. vinosum was recombinantly expressed, purified and crystallized. The crystals belonged to space group C2, with unit-cell parameters a = 79.2, b = 69.9, c = 57.9 Å, [beta] = 129.3°, and diffracted to a resolution of 1.98 Å.


Accepted 27 August 2014

The structure of lactoferrin-binding protein B from Neisseria meningitidis suggests roles in iron acquisition and neutralization of host defences

C. L. Brooks, E. Arutyunova and M. J. Lemieux

Synopsis: The crystal structure of the N-terminal domain of lactoferrin-binding protein B (LbpB) from N. meningitidis is reported. Docking of the structure with lactoferrin suggests roles for LbpB in both host iron acquisition and neutralization of the toxic effects of the lactoferricin peptide.


Accepted 26 August 2014

Enhancing ubiquitin crystallization through surface-entropy reduction

P. J. Loll, P. Xu, J. T. Schmidt and S. L. Melideo

Synopsis: All seven possible single-site lysine-to-serine mutations of ubiquitin were tested for crystallization behavior and were found to yield crystallization `hit rates' varying by two orders of magnitude. High-resolution structures of three mutants revealed that mutations can exert both promoting and permissive effects on crystallization.


Accepted 26 August 2014

Crystallization and preliminary crystallographic analysis of poly(3-hydroxybutyrate) depolymerase from Bacillus thuringiensis

Y.-L. Wang, Y.-T. Lin, C.-L. Chen, G.-C. Shaw and S.-H. Liaw

Synopsis: The first crystal structure for an intracellular poly(3-hydroxybutyrate) depolymerase at 1.42 Å resolution was determined by molecular replacement using a search model with 24% sequence identity.


Accepted 25 August 2014

Purification, crystallization and preliminary X-ray crystallographic studies of Drep2 CIDE domain

S. M. Lee and H. H. Park

Synopsis: The CIDE domain of Drep2 was purified and crystallized in space group P212121, with unit-cell parameters a = 50.28, b = 88.70, c = 113.37 Å. The crystals diffracted to a resolution of 3.3 Å.


Accepted 25 August 2014

Crystallization and preliminary X-ray diffraction analysis of a novel sphingomyelinase D from Loxosceles gaucho venom

A. Ullah, G. S. Magalhães, R. Masood, R. B. Mariutti, M. A. Coronado, M. T. Murakami, K. C. Barbaro and R. K. Arni

Synopsis: The SMase D from L. gaucho venom was expressed, purified, crystallized and diffraction data were collected to 2.6 Å resolution.


Accepted 21 August 2014

Structure of an Aspergillus fumigatus old yellow enzyme (EasA) involved in ergot alkaloid biosynthesis

A. S. Chilton, A. L. Ellis and A. L. Lamb

Synopsis: The structure of the A. fumigatus old yellow enzyme EasA was determined to 1.8 Å resolution. This enzyme catalyzes the reduction of chanoclavine-I aldehyde to dihydrochanoclavine aldehyde and works in conjunction with festuclavine synthase at the branchpoint for ergot alkaloid pathways.


Accepted 20 August 2014

Crystallization and preliminary X-ray diffraction analysis of cyclolavandulyl diphosphate synthase, a new member of the cis-isoprenyl diphosphate synthase superfamily

T. Tomita, T. Ozaki, K. Matsuda, M. Nishiyama and T. Kuzuyama

Synopsis: Cyclolavandulyl diphosphate synthase, which catalyzes both the condensation of two molecules of C5 dimethylallyl diphosphate and the subsequent cyclization, has been crystallized and X-ray diffraction data have been collected and analyzed.


Accepted 18 August 2014

Expression, crystallization and preliminary X-ray analysis of McbB, a multifunctional enzyme involved in [beta]-carboline skeleton biosynthesis

H. Wang, H. Zhang, Y. Mi, J. Ju, Q. Chen and H. Zhang

Synopsis: McbB, a multifunctional enzyme responsible for catalysing Pictet-Spengler cyclization, decarboxylation and oxidation reactions in the biosynthesis of [beta]-carboline, was expressed and crystallized. The crystals belonged to the monoclinic space group P21, with unit-cell parameters a = 66.06, b = 85.48, c = 106.19 Å, [alpha] = 90.00, [beta] = 106.77, [gamma] = 90.00°.


Accepted 18 August 2014

Structure of homoserine O-acetyltransferase from Staphylococcus aureus: the first Gram-positive ortholog structure

B. Thangavelu, A. Pavlovski and R. Viola


Accepted 18 August 2014

Crystallization and preliminary X-ray diffraction analyses of the redox-controlled complex of terminal oxygenase and ferredoxin components in the Rieske nonhaem iron oxygenase carbazole 1,9a-dioxygenase

J. Matsuzawa, H. Aikawa, T. Umeda, Y. Ashikawa, C. Suzuki-Minakuchi, Y. Kawano, Z. Fujimoto, K. Okada, H. Yamane and H. Nojiri

Synopsis: A crystal was obtained of the complex between reduced terminal oxygenase and oxidized ferredoxin components of carbazole 1,9a-dioxygenase. The crystal belonged to space group P21 and diffracted to 2.25 Å resolution.


Accepted 14 August 2014

Crystallization and preliminary X-ray diffraction analysis of Lin1840, a putative [beta]-glucosidase from Listeria innocua

M. Nakajima, R. Yoshida, A. Miyanaga and H. Taguchi

Synopsis: Lin1840 from L. innocua has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to a resolution of 1.8 Å.


Accepted 13 August 2014

Crystallization of the Ets1-Runx1-CBF[beta]-DNA complex formed on the TCR[alpha] gene enhancer

M. Shiina, K. Hamada, T. Inoue-Bungo, M. Shimamura, S. Baba, K. Sato and K. Ogata

Synopsis: The Ets1-Runx1-CBF[beta]-DNA complex, a higher-order TF-DNA complex formed on the T-cell receptor [alpha] gene enhancer, was crystallized.


Accepted 13 August 2014

Crystallization and preliminary X-ray crystallographic studies of CrArsM, an arsenic(III) S-adenosylmethionine methyltransferase from Chlamydomonas reinhardtii

C. Packianathan, J. K. Pillai, A. Riaz, P. Kandavelu, B. Sankaran and B. P. Rosen

Synopsis: The crystallization and preliminary crystallographic analysis of CrArsM, an arsenic(III) S-adenosylmethionine methyltransferase from C. reinhardtii, is described.


Accepted 13 August 2014

Crystallization and preliminary X-ray diffraction analysis of the BRPF1 bromodomain in complex with its H2AK5ac and H4K12ac histone-peptide ligands

M. Y. Lubula, A. Poplawaski and K. C. Glass


Accepted 13 August 2014

Cloning, expression, purification and preliminary X-ray analysis of EstN2, a novel archaeal [alpha]/[beta]-hydrolase from Candidatus Nitrososphaera gargensis

H. Kaljunen, J. Chow, W. R. Streit and J. Mueller-Dieckmann

Synopsis: The chemolithoautotrophic nitrifying thaumarchaeon Ca. Nitrososphaera gargensis with unusual metabolic pathways was enriched by cultivation over six years. A novel [alpha]/[beta]-hydrolase was identified, isolated and crystallized for further functional analysis.


Accepted 12 August 2014

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of fructose-1,6-bisphosphate aldolase from Escherichia coli

L. Zhang, Z. Guo, J. Huang, M. Liu, Y. Wang and C. Ji

Synopsis: FBPA I from E. coli was expressed, purified and crystallized. The crystals diffracted to 2.0 Å resolution and belonged to space group C2.


Accepted 12 August 2014

Crystallization and preliminary X-ray analysis of the hypothetical deaminase RPB_0146 from Rhodopseudomonas palustris HaA2

G. Zhang, D. Yu, G. Yang, H. Dong, T. Zhang and X. Liu

Synopsis: The hypothetical deaminase RPB_0146 from R. palustris HaA2 was expressed in E. coli and purified. Crystallization and preliminarily X-ray crystallographic analysis of the recombinant deaminase were performed.


Accepted 12 August 2014

A drunken search in crystallization space

V. J. Fazio, T. S. Peat and J. Newman

Synopsis: We look to the PDB to find data to answer the question `How well do our current screens cover crystallization space?' and to try to find answers to the more general question about the most efficient strategy to employ in a crystallization campaign.


Accepted 11 August 2014

Purification and X-ray crystallographic analysis of 7-keto-8-aminopelargonic acid (KAPA) synthase from Mycobacterium smegmatis

S. Fan, D. Li, J. Fleming, Y. Hong, T. Chen, L. Zhou, L. Bi, D. Wang, X. Zhang and G. Chen

Synopsis: The cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of 7-keto-8-aminopelargonic acid (KAPA) synthase from M. smegmatis are described..


Accepted 7 August 2014

Human carbonic anhydrase II-cyanate inhibitor complex: putting the debate to rest

D. West, M. A. Pinard, C. Tu, D. N. Silverman and R. McKenna

Synopsis: A controversy has existed in the literature between crystallographic and spectroscopic data on the binding mode of cyanate to carbonic anhydrase II (CA II). To settle this ambiguity, the X-ray crystal structures of the complexes of wild-type and V207I variant CA II with cyanate were redetermined to 1.7 and 1.5 Å resolution, respectively. The data clearly support that cyanate binds directly to the catalytic zinc and not as an outer sphere ligand mimicking the binding of CO2.


Accepted 5 August 2014

Expression, crystallization and preliminary X-ray crystallographic analysis of cystathionine [beta]-lyase from Acinetobacter baumannii OXA-23

D.-Q. Nguyen, H.-P.-T. Ngo, Y.-J. Ahn, S. H. Lee and L.-W. Kang

Synopsis: Cystathionine [beta]-lyase from multidrug-resistant A. baumannii OXA-23 (AbCBL), an enzyme involved in the methionine-metabolism pathway and a novel antibacterial drug target, was cloned, expressed, purified and crystallized. Preliminary X-ray crystallography was performed to analyse the AbCBL crystal.


Accepted 2 August 2014

Crystallization and preliminary X-ray diffraction studies of an L-amino-acid oxidase from Lachesis muta venom

A. Ullah, R. Masood, P. J. Spencer, M. T. Murakami and R. K. Arni

Synopsis: An L-amino-acid oxidase from L. muta venom was crystallized and diffraction data were collected to 3.0 Å resolution.


Accepted 2 August 2014

Cloning, expression, purification, crystallization and preliminary X-ray diffraction of a lysine-specific permease from Pseudomonas aeruginosa

E. D. Nji, D. Li, D. A. Doyle and M. Caffrey

Synopsis: The integral membrane lysine permease from P. aeruginosa was cloned and overexpressed in E. coli as a GFP-fusion protein. Crystals of the tag-less transporter diffracted to 7.5 Å resolution.


Accepted 29 July 2014

Crystallization and preliminary X-ray diffraction analysis of the Pax9 paired domain bound to a DC5 enhancer DNA element

K. Narasimhan, A. Hilbig, B. Udayasuryan, S. Jayabal, P. R. Kolatkar and R. Jauch

Synopsis: Crystals of the Pax9 paired domain bound to a DC5 enhancer DNA element that diffract anisotropically to 3.0 Å resolution are reported.


Accepted 22 July 2014

Purification, crystallization and preliminary X-ray crystallographic analysis of human IL-18 and its extracellular complexes

T. Kimura, N. Tsutsumi, K. Arita, M. Ariyoshi, H. Ohnishi, N. Kondo, M. Shirakawa, Z. Kato and H. Tochio

Synopsis: IL-18 and its two receptors, IL-18R[alpha] and IL-18R[beta], were purified for crystallization and biochemical analysis. IL-18 was crystallized in free, IL-18R[alpha]-bound and IL-18R[alpha]/IL-18R[beta]-bound states and complete X-ray data sets suitable for further structural analysis were obtained from each crystal.


Accepted 10 July 2014

Expression, purification and preliminary crystallographic analysis of the cryptic polo-box domain of Caenorhabditis elegans ZYG-1

E. Shimanovskaya and G. Dong

Synopsis: The cryptic polo-box (CPB) domain of the polo-like kinase ZYG-1 from C. elegans was cloned, overexpressed, purified and crystallized. Anomalous X-ray diffraction data were collected to 2.54 Å resolution.


Accepted 10 June 2014

Crystallization and preliminary X-ray diffraction analysis of the S-adenosylhomocysteine hydrolase (SAHH) from Thermotoga maritima

M. He, Y. Zheng, C.-H. Huang, G. Qian, X. Xiao, T.-P. Ko, W. Shao and R.-T. Guo




































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