forthcoming articles in Acta Crystallographica Section F

The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.

This list will generally be short, as papers in this journal are published online as soon as proofs are returned.

See also Forthcoming articles in all IUCr journals.


Accepted 28 July 2014

Crystallization and preliminary X-ray diffraction analysis of Xaa-Pro dipeptidase from Xanthomonas campestris

A. Kumar, V. N. Are, B. Ghosh, U. Agrawal, S. N. Jamdar, R. D. Makde and S. M. Sharma

Synopsis: The expression, purification and crystallization of Xaa-Pro dipeptidase from Xanthomonas campestris (GenBank accession, NP_637763), and its preliminary X-ray diffraction analysis to 1.83 Å resolution are reported here.


Accepted 24 July 2014

Structure of Toxoplasma gondii fructose 1,6 bisphosphate aldolase

L. E. Boucher and J. Bosch

Synopsis: The structure of Toxoplasma gondii fructose 1,6 bisphosphate aldolase, a glycolytic enzyme and structural component of the invasion machinery, was determined to a resolution of 2.0 Å.


Accepted 23 July 2014

Purification, crystallization and preliminary crystallographic analysis of 16S rRNA methyltransferase RsmI from Escherichia coli

M. Zhao, L. Wang, H. Zhang, Y. Dong, Y. Gong, L. Zhang and J. Wang

Synopsis: Preliminary crystallographic analysis of 16S rRNA methyltransferase RsmI from Escherichia coli.


Accepted 22 July 2014

Purification, crystallization and preliminary X-ray crystallographic analysis of human IL-18 and its extracellular complexes

T. Kimura, N. Tsutsumi, K. Arita, M. Ariyoshi, H. Ohnishi, N. Kondo, M. Shirakawa, Z. Kato and H. Tochio

Synopsis: IL-18 and its two receptors, IL-18R[alpha] and IL-18R[beta], were purified for crystallization and biochemical analysis. IL-18 was crystallized in the free, IL-18R[alpha]-bound and IL-18R[alpha]/IL-18R[beta]-bound states and complete X-ray data sets suitable for further structural analysis were obtained from each crystal.


Accepted 22 July 2014

Crystallization and preliminary X-ray analysis of the C-terminal fragment of Ski7 from Saccharomyces cerevisiae

J.-Y. Lee, S. H. Park, B.-C. Jeong and H. K. Song

Synopsis: C-terminal domains 2 and 3 of Ski7 from Saccharomyces cerevisiae were purified and crystallized. The crystals diffract to 2.0 Å resolution.


Accepted 15 July 2014

Structure of glutathione S-transferase-1 from the major human hookworm parasite Necator americanus (Na-GST-1) in complex with glutathione

O. A. Asojo and C. Ceccarelli

Synopsis: The structure of Na-GST-1 in complex with GST is reported.


Accepted 15 July 2014

Structure of a DsbF homologue from Corynebacterium diphtheriae

S.-H. Um, J.-S. Kim, K. Lee and N.-C. Ha


Accepted 15 July 2014

Expression, purification, crystallization and preliminary X-ray analysis of glucose-1-phosphate uridylyltransferase (GalU) from Erwinia amylovora

M. Toccafondi, M. Cianci and S. Benini

Synopsis: The glucose-1-phosphate uridylyltransferase from E. amylovora has been cloned, expressed, purified and crystallized. Data were collected to 2.46 Å resolution in space group P62 and the structure was solved by molecular replacement using the structure of the enzyme from E. coli.


Accepted 11 July 2014

Carboplatin binding to histidine

S. W. M. Tanley, K. Diederichs, L. M. J. Kroon-Batenburg, C. Levy, A. M. M. Schreurs and J. R. Helliwell

Synopsis: An X-ray crystal structure showing the binding of purely carboplatin to histidine in a model protein has finally been obtained. This required extensive crystallization trials and various novel crystal structure analyses.


Accepted 10 July 2014

Expression, purification and preliminary crystallographic analysis of the cryptic polo-box domain of Caenorhabditis elegans ZYG-1

E. Shimanovskaya and G. Dong

Synopsis: The cryptic polo-box (CPB) domain of the polo-like kinase ZYG-1 from C. elegans was cloned, overexpressed, purified and crystallized. Anomalous X-ray diffraction data were collected to 2.54 Å resolution.


Accepted 8 July 2014

Purification, characterization and preliminary X-ray crystallographic studies of monodehydroascorbate reductase from Oryza sativa L. japonica

H. Do, I.-S. Kim, Y.-S. Kim, S.-Y. Shin, J.-J. Kim, J.-E. Mok, S.-I. Park, A. R. Wi, H. Park, J. H. Lee, H.-S. Yoon and H.-W. Kim

Synopsis: Monodehydroascorbate reductase (OsMDHAR) from O. sativa L. japonica was expressed as the active form using E. coli strain NiCo21 (DE3). X-ray diffraction data were collected to 1.9 Å resolution.


Accepted 5 July 2014

Crystallization of the C-terminal redox domain of the sulfur-assimilatory enzyme APR1 from Arabidopsis thaliana

F.-F. Chen, Y.-Y. Chang, C.-C. Cho and C.-H. Hsu

Synopsis: The redox domain of APR1 from A. thaliana was crystallized in the tetragonal space group P43212 or P41212 and diffraction data were collected to 2.70 Å resolution.


Accepted 5 July 2014

Crystallization and preliminary X-ray crystallographic analysis of YfcM: an important factor for EF-P hydroxylation

K. Kobayashi, T. Suzuki, N. Dohmae, R. Ishitani and O. Nureki

Synopsis: E. coli YfcM was expressed, purified and crystallized. Crystals of YfcM were obtained by the in situ proteolysis crystallization method. Using these crystals, an X-ray diffraction data set was collected at 1.45 Å resolution.


Accepted 5 July 2014

Expression, purification, crystallization and preliminary crystallographic analysis of human myotubularin-related protein 3

J. Y. Son, J. U. Lee, K.-Y. Yoo, W. Shin, D.-W. Im, S. J. Kim, S. E. Ryu and Y.-S. Heo

Synopsis: Human myotubularin-related protein 3 was cloned, expressed, purified and crystallized. X-ray diffraction data were collected to 3.3 Å resolution.


Accepted 3 July 2014

Structure of nucleoside diphosphate kinase from pacific shrimp (Litopenaeus vannamei) in binary complexes with purine and pyrimidine nucleoside diphosphates

A. A. López-Zavala, I. E. Quintero-Reyes, J. S. Carrasco-Miranda, V. Stojanoff, A. Weichsel, E. Rudiño-Piñera and R. R. Sotelo-Mundo


Accepted 2 July 2014

High-resolution crystal structures of alternate forms of the human CD44 hyaluronan-binding domain reveal a site for protein interaction

L.-K. Liu and B. Finzel

Synopsis: Two alternate crystal forms of the human CD44 hyaluronan-binding domain, one hexagonal and one monoclinic, have been determined at 1.60 and 1.08 Å resolution, respectively. A short peptide found in the monoclinic form may identify a site of CD44 for protein-protein interactions.


Accepted 2 July 2014

Cloning, purification, crystallization and preliminary X-ray studies of a carbohydrate-binding module (CBM_E1) derived from a sugarcane soil metagenome

B. M. Campos, T. M. Alvarez, M. V. Liberato, I. Polikarpov, H. J. Gilbert, A. C. M. Zeri and F. M. Squina


Accepted 2 July 2014

Microseed matrix screening for optimization in protein crystallization: what have we learned?

A. D'Arcy, T. Bergfors, S. W. Cowan-Jacob and M. Marsh

Synopsis: Microseed matrix screening is increasingly successful in the optimization of protein crystallization leads.


Accepted 1 July 2014

Cloning, purification and preliminary crystallographic analysis of the Helicobacter pylori pseudaminic acid biosynthesis N-acetyltransferase PseH

Y. C. Liu, A. I. Ud-Din and A. Roujeinikova

Synopsis: The N-acetyltransferase PseH from the H. pylori pseudaminic acid biosynthesis pathway has been purified and co-crystallized with acetyl-CoA by the hanging-drop vapour-diffusion method. A diffraction data set has been collected to 2.5 Å resolution.


Accepted 26 June 2014

Structural basis for the inhibition of poly(ADP-ribose) polymerases 1 and 2 by BMN 673, a potent inhibitor derived from dihydropyridophthalazinone

M. Aoyagi-Scharber, A. S. Gardberg, B. K. Yip, B. Wang, Y. Shen and P. A. Fitzpatrick

Synopsis: BMN 673, a novel PARP1/2 inhibitor in clinical development with substantial tumor cytotoxicity, forms extensive hydrogen-bonding and [pi]-stacking in the nicotinamide pocket, with its unique disubstituted scaffold extending towards the less conserved edges of the pocket. These interactions might provide structural insight into the ability of BMN 673 to both inhibit catalysis and affect DNA-binding activity.


Accepted 23 June 2014

Structural analysis of the GH43 enzyme Xsa43E from Butyrivibrio proteoclasticus

M. Till, D. Goldstone, G. Card, G. T. Attwood, C. D. Moon and V. L. Arcus


Accepted 23 June 2014

Crystallization and preliminary X-ray diffraction analysis of the periplasmic domain of the Escherichia coli aspartate receptor Tar and its complex with aspartate

T. Mise, H. Matsunami, F. Samatey and I. Maruyama


Accepted 21 June 2014

Crystallization and preliminary X-ray analysis of the periplasmic domain of FliP, an integral membrane component of the bacterial flagellar type III protein-export apparatus

T. Fukumura, Y. Furukawa, T. Kawaguchi, Y. Saijo-Hamano, K. Namba, K. Imada and T. Minamino

Synopsis: The periplasmic domain of FliP of the bacterial flagellar type III export apparatus has been expressed, purified and crystallized, and the crystals have been characterized by X-ray diffraction.


Accepted 21 June 2014

Cloning, expression, purification, crystallization and preliminary crystallographic analysis of the C-terminal domain of Par-4 (PAWR)

U. K. Tiruttani Subhramanyam, J. Kubicek, U. B. Eidhoff and J. Labahn

Synopsis: The cloning, overexpression, purification, crystallization and preliminary crystallographic analysis of the C-terminal domain of prostate apoptosis response-4 protein (Par-4) are presented.


Accepted 20 June 2014

Purification, crystallization and preliminary X-ray crystallographic analysis of glycosyltransferase-1 from Bacillus cereus

Y.-C. Hsieh, H.-H. Chiu, Y.-C. Huang, H.-K. Fun, C.-Y. Lu, Y.-K. Li and C.-J. Chen

Synopsis: The purification, crystallization and preliminary X-ray crystallographic analysis of B. cereus glycosyltransferase-1 are reported.


Accepted 18 June 2014

Molecular replacement with a large number of molecules in the asymmetric unit

C. Jobichen and K. Swaminathan

Synopsis: A survey of the Protein Data Bank for entries with a large number of molecules in the asymmetric unit was performed and the strategies used for molecular replacement are described.


Accepted 14 June 2014

Chemical conversion of cisplatin and carboplatin with histidine in a model protein crystallized under sodium iodide conditions

S. W. M. Tanley and J. R. Helliwell

Synopsis: Crystals of HEWL with cisplatin and HEWL with carboplatin grown in sodium iodide conditions both show a partial chemical transformation of cisplatin or carboplatin to a transiodoplatin (PtI2X2) form. The binding is only at the N[delta] atom of His15. A further Pt species (PtI3X) is also seen, in both cases bound in a crevice between symmetry-related protein molecules.


Accepted 14 June 2014

The binding of platinum hexahalides (Cl, Br and I) to hen egg-white lysozyme and the chemical transformation of the PtI6 octahedral complex to a PtI3 moiety bound to His15

S. W. M. Tanley, L.-V. Starkey, L. Lamplough, S. Kaenket and J. R. Helliwell

Synopsis: The platinum hexahalides have an octahedral arrangement of six halogen atoms bound to a Pt centre, thus having an octahedral shape that could prove to be useful in poor electron-density maps. In a detailed characterization, PtI6 chemically transformed to a square-planar PtI3 complex bound to the N[delta] atom of His15 of HEWL was also observed, which was not observed for PtBr6 or PtCl6.


Accepted 10 June 2014

Crystallization and preliminary X-ray diffraction analysis of the S-adenosylhomocysteine hydrolase (SAHH) from Thermotoga maritima

M. He, Y. Zheng, C.-H. Huang, G. Qian, X. Xiao, T.-P. Ko, W. Shao and R.-T. Guo


Accepted 1 June 2014

Crystallization, preliminary X-ray crystallographic and cryo-electron microscopy analysis of a bifunctional enzyme fucokinase/L-fucose-1-P-guanylyltransferase from Bacteroides fragilis

C. Cheng, J. Gu, J. Su, W. Ding, J. Yin, W. Liang, X. Yu, J. Ma, P. G. Wang, Z. Xiao and Z.-J. Liu

Synopsis: The expression, purification, crystallization, preliminary X-ray crystallographic and cryo-EM analysis of the bifunctional enzyme fucokinase/L-fucose-1-P-guanylyltransferase are reported. A diffraction data set was collected to 3.7 Å resolution from the full-length native protein crystal using synchrotron X-rays. The preliminary cryo-EM image showed two parallel disc-shaped objects with a diameter of 10 nm.




































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