The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.
This list will generally be short, as papers in this journal are published online as soon as proofs are returned.
See also Forthcoming articles in all IUCr journals.
Synopsis: X-ray data of eight different Dscam1 Ig1-4 isoforms were collected to 1.9-4.0 Å, respectively. These structures will provide the opportunity to do extensive structural comparison of different Dscam1 isoforms and shed insights into its specificity.
Synopsis: 3-Ketoacyl-CoA thiolase from R. eutropha (ReH16_A1887), an enzyme involved in -oxidation degradative pathways, has been crystallized, and X-ray diffraction data have been collected from the crystal and analyzed.
Synopsis: Nicotinamide adenine dinucleotide synthetase (NAD synthetase) from methicillin-resistant S. aureus was cloned, purified, crystallized and X-ray diffraction data were collected to a resolution of 2.0 Å.
Synopsis: The Acinetobacter baumannii biofilm-forming CSU fimbriae are assembled via the archaic CsuC/CsuD chaperone/usher pathway. To determine the structural basis for the biogenesis of CSU fimbriae, native and SeMet-substituted CsuC-CsuA/B chaperone-major subunit pre-assembly complexes were produced in Escherichia coli, purified and crystallized. Initial phases were derived from single anomalous diffraction (SAD) experiment using the SeMet derivative.
Synopsis: Latent and active aurone synthase purified from petals of C. grandiflora (cgAUS1) were crystallized. The crystal quality of recombinantly expressed latent cgAUS1 was significantly improved by co-crystallization with the polyoxotungstate Na6[TeW6O24] within the liquid-liquid phase-separation zone.
Synopsis: Crystal structure of the Mre11 nuclease dimer from the thermophilic eukaryote Chaetomium thermophilum. Crystals of Chaetomium thermophilum Mre11, which plays an important role in DNA double-strand break repair, diffracted up to 2.8 Å and the structure was solved by the molecular replacement technique.
Synopsis: This article describes preliminary crystallographic data for the I26A/N52A mutant of nonstructural protein 15 from human coronavirus 229E.
Synopsis: The crystal structure of the M. tuberculosis MarR family protein Rv0880 is described.
Synopsis: A Cmr1-deficient functional Cmr (Cmr1) complex composed of P. furiosus Cmr2-Cmr3, A. fulgidus Cmr4-Cmr5-Cmr6 and the 39-mer P. furiosus 7.01-crRNA was cocrystallized with single-stranded DNA complementary to the crRNA guide. X-ray diffraction data for the crystals were collected to 2.1 Å resolution using a synchrotron-radiation source.
Synopsis: The X-ray structure of cyanide-bound bovine heart cytochrome c oxidase in the fully oxidized state was determined at 2.0 Å resolution. This structure shows that a cyanide ion bridges the two metals in a nearly symmetric end-on fashion.
Synopsis: A MepR-like transcription factor, BC0657, from B. cereus was expressed, purified and crystallized. X-ray diffraction data for the BC0657 crystals were collected to a resolution of 2.05 Å.
Synopsis: The structure of THB1, a monomeric 2/2 hemoglobin involved in nitrogen metabolism in C. reinhardtii, was solved to 1.9 Å resolution. The heme is coordinated by the proximal histidine and a distal lysine.
Synopsis: Crystals of a complex between human B7-H6, a tumor cell ligand for NKp30, and an inhibitory monoclonal antibody have been obtained. The crystals diffracted to 2.5 Å resolution.
Synopsis: Binding studies and an activity assay were performed for heat-shock protein 90 from O. sativa. The N-terminal domain of Hsp90 was crystallized and its structure was determined in complex with AMPPCP.
Synopsis: Contact-dependent growth inhibition (CDI) is an important mechanism of inter-cellular competition between Gram-negative bacteria. The X-ray crystal structure of an immunity protein from N. meningitidis MC58 is presented together with a structural model of the toxin-immunity protein complex.
Synopsis: The crystal structure of the LRR domain from PP32A has been solved to 1.6 Å resolution.
Synopsis: A -1,3-glucanase from the thermophilic fungus C. thermophilum was overexpressed in P. pastoris, purified and crystallized. In situ diffraction at a synchrotron and seeding were used to obtain reproducible crystals. A complete data set to 2.0 Å resolution was collected using an in-house X-ray generator.
Synopsis: The antibiotic-resistance protein NSR from S. agalactiae was crystallized in two different crystal forms, which diffracted X-rays to a maximum resolution of 2.2 Å.
Synopsis: In order to understand the structure-function relationship of 3-deoxy-D-manno-oct-2-ulosonic acid 8-phosphate phosphatase (YrbI) from B. pseudomallei, its crystallographic study has been performed. Here, the cloning, overexpression, purification, crystallization and preliminary X-ray study of this enzyme are reported.
Synopsis: The CFA/III minor pilin subunit, CofB, has been crystallized. An N-terminal hydrophobic portion of CofB was truncated to enable initial crystallization. Dehydration of preliminary crystals yielded higher resolution diffraction patterns.
Synopsis: A. thaliana BAG5 (AtBAG5) and calmodulin were expressed and purified separately and then co-crystallized. The preliminary X-ray diffraction studies of the protein complex are reported in order to provide structural insights into the mode of interaction between AtBAG5 and calmodulin.
Synopsis: Crystallization and diffraction studies of the HIRAN domain of human HLTF are reported.
Synopsis: Diffraction data from crystals of zebrafish (D. rerio) 2-microglobulin type I and type II (Dare-2m-I and Dare-2m-II) were collected to 1.6 and 1.9 Å resolution, respectively.
Synopsis: The crystal packing of N,N'-diacetylchitobiose deacetylase from P. furiosus varied depending on the presence of cadmium and whether the native protein or its selenomethionine derivative was used. The cadmium complex was involved in the crystal contacts in Cd-containing conditions.
Synopsis: The crystal structure of the catalytic domain of human dual-specificity phosphatase 7 (C232S) is presented at 1.67 Å resolution.
Synopsis: Superoxide dismutases (SODs) catalyze the conversion of superoxide to molecular oxygen, thus protecting organisms against toxic radicals. Iron-dependent SODs from T. cruzi, L. major and B. bovis exist as multiple isoforms with distinct structural features.
Synopsis: Hookworm platelet inhibitor belongs to the cysteine-rich/antigen-5/pathogenesis-related 1 (CAP) protein family. It has been linked to integrin-antagonist, antiplatelet activity found in extracts of A. caninum hookworms.
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