forthcoming articles in Acta Crystallographica Section F

The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.

This list will generally be short, as papers in this journal are published online as soon as proofs are returned.

See also Forthcoming articles in all IUCr journals.

Accepted 23 July 2015

Expression, purification, crystallization and preliminary X-ray diffraction studies of the molecular chaperone prefoldin from Homo sapiens

Y. Aikawa, H. Kida, Y. Nishitani and K. Miki

Synopsis: Six subunits of human prefoldin were overexpressed in E. coli. Prefoldin was reconstituted, purified and crystallized. X-ray diffraction data were collected at 4.7 Å resolution.

Accepted 20 July 2015

Structure of the SPRY domain of human DDX1 helicase, a putative interaction platform within a DEAD-box protein

J. Kellner and A. Meinhart

Synopsis: The structure of the SPRY domain of the human RNA helicase DDX1 was determined at 2.0 Å resolution. The SPRY domain provides a putative protein-protein interaction platform within DDX1 that differs in its structure and conserved regions from other SPRY domains.

Accepted 15 July 2015

Structure of the N-terminal dimerization domain of CEACAM7

D. A. Bonsor, D. Beckett and E. J. Sundberg

Synopsis: The crystal structure of the dimerization domain of CEACAM7, a human cell adhesion protein, was determined at 1.47 Å resolution and the Kdimerization was measured by analytical ultracentrifugation.

Accepted 14 July 2015

Cleavage of nicotinamide adenine dinucleotide by the ribosome-inactivating protein from Momordica charantia

M. Vinkovic, G. Dunn, G. E. Wood, J. Husain, S. P. Wood and R. Gill

Synopsis: High-resolution structure analyses of the N-glycosidase ribosome-inactivating protein from seeds of M. charantia shows that the enzyme preferentially cleaves nicotinamide from the oxidized form of the coenzyme NADP.

Accepted 14 July 2015

Crystals of Arp2/3 complex in two new space groups with structural information about actin-related protein 2 and potential WASp binding sites

C. T. Jurgenson and T. D. Pollard

Synopsis: The actin-polymerizing protein Arp2/3 complex was crystallized in two new space groups. The new electron-density maps from these new crystal forms reveal some details that may help to elucidate how nucleation-promoting factors bind to the complex.

Accepted 7 July 2015

Crystal structure analysis of c4763, a uropathogenic Escherichia coli-specific protein

H. Kim, J. Choi, D. Kim and K. K. Kim

Synopsis: The crystal structure of c4763, a uropathogenic E. coli-specific protein, at a resolution of 1.45 Å reveals that it is a structural homologue of allophanate hydrolase, which is involved in the urea cycle. These results suggest that c4763 might be involved in urea utilization, which is necessary for bacterial survival in the urinary tract.

Accepted 3 July 2015

Crystallization and crystallographic studies of kallistatin

F. Lin, A. Zhou and Z. Wei

Synopsis: The crystallization of human kallistatin in the relaxed conformation is reported.

Accepted 3 July 2015

Cloning, expression, purification and crystallization of a pair of novel virulence factors, SghA and SghR, from Agrobacterium tumefaciens

F. Ye, C. Wang, Q. Fu, L. Zhang and Y. Gao

Synopsis: The crystallization of the novel virulence factors SghA and SghR is reported.

Accepted 1 July 2015

Structure of RizA, an L-amino-acid ligase from Bacillus subtilis

W. Kagawa, T. Arai, S. Ishikura, K. Kino and H. Kurumizaka

Synopsis: The structure of the L-amino-acid ligase RizA was determined at 2.8 Å resolution.

Accepted 1 July 2015

Formulation screening by differential scanning fluorimetry: how often does it work?

M. Ristic, N. Rosa, S. A. Seabrook and J. Newman

Synopsis: Over 250 proteins have been subjected to a standard dye-based thermal melt analysis, and the results suggest that at least a third of soluble protein samples can be made significantly more stable by changing the composition of their formulation.

Accepted 1 July 2015

X-ray crystallographic studies of the middle part of the human synaptonemal complex protein 1 coiled-coil domain

H. H. Park

Synopsis: The middle of syneptonemal complex protein 1, which is composed of a coiled-coil domain, was purified and crystallized. The crystals were found to belong to space group I4, with unit-cell parameters a = 41.95, b = 41.95, c = 318.78 Å. The crystals were obtained at 293 K and diffracted to a resolution of 3.0 Å.

Accepted 30 June 2015

Crystallographic studies of the structured core domain of Knr4 from Saccharomyces cerevisiae

S. Julien, P. Tondl, F. Durand, A. Dagkessamanskaia, H. van Tilbeurgh, J.-M. François, L. Mourey, D. Zerbib, H. Martin-Yken and L. Maveyraud

Synopsis: The potentially structured core domain of the intrinsically disordered protein Knr4, residues 80-340, was expressed and crystallized using native and selenomethionine-labelled proteins.

Accepted 30 June 2015

Structure of the 14-3-3[zeta]-LKB1 fusion protein provides insight into a novel ligand-binding mode of 14-3-3

S. Ding, R. Zhou and Y. Zhu

Synopsis: The crystal structure of a 14-3-3[zeta]-LKB1 fusion protein provides a novel potential binding mode of 14-3-3 to its ligands.

Accepted 29 June 2015

High-resolution crystal structure of cAMP-dependent protein kinase from Cricetulus griseus

D. Kudlinzki, V. L. Linhard, K. Saxena, S. Sreeramulu, S. Gande, U. Schieborr, M. Dreyer and H. Schwalbe

Synopsis: The high-resolution crystal structure of a cAMP-dependent protein kinase is reported.

Accepted 27 June 2015

A sucrose-binding site provides a lead towards an isoform-specific inhibitor of the cancer-associated enzyme carbonic anhydrase IX

M. A. Pinard, M. Aggarwal, B. Mahon, C. Tu and R. McKenna

Synopsis: An engineered human carbonic anhydrase IX (CA IX) mimic was co-crystallized with the disaccharide sucrose. Its binding provides a novel `interaction' site that can be used to design targeted inhibitors of CA IX, a key marker of hypoxic tumors.

Accepted 26 June 2015

Structure of GUN4 from Chlamydomonas reinhardtii

S. Tarahi Tabrizi, D. B. Langley, S. J. Harrop, A. P. Duff and R. D. Willows

Synopsis: The structure of a eukaryotic GUN4 is compared with prokaryotic GUN4 structures.

Accepted 24 June 2015

Structural analysis of Clostridium aceto­butylicum ATCC 824 glycoside hydrolase from CAZy family GH105

K. L. Germane, M. D. Servinsky, E. S. Gerlach, C. J. Sund and M. M. Hurley

Synopsis: The crystal structure of the protein product of the C. acetobutylicum ATCC 824 gene CA_C0359 is structurally similar to YteR, an unsaturated rhamnogalacturonyl hydrolase from B. subtilis strain 168. Substrate modeling and electrostatic studies of the active site of the structure of CA_C0359 suggests that the protein can now be considered to be part of CAZy glycoside hydrolase family 105.

Accepted 22 June 2015

Crystallization and crystallographic analysis of branching enzymes from Cyanothece sp. ATCC 51142

M. Hayashi, R. Suzuki, C. Colleoni, S. G. Ball, N. Fujita and E. Suzuki

Synopsis: The biochemical characterization and X-ray crystallographic analysis of branching enzymes from the cyanobacterium Cyanothece sp. ATCC 51142 are reported.

Accepted 22 June 2015

Structure of human Roquin-2 and its complex with constitutive-decay element RNA

S. Sakurai, U. Ohto and T. Shimizu

Synopsis: Roquin mediates mRNA degradation by recognizing the constitutive-decay element. Crystal structures of the ROQ domain of Roquin-2 were determined in ligand-free and RNA-bound forms, in which Roquin-2 recognized RNA forming a stem-loop structure.

Accepted 18 June 2015

Expression, purification, crystallization and X-ray crystallographic analysis of the periplasmic binding protein VatD from Vibrio vulnificus M2799

N. Miyano, T. Igarashi, H. Kawano, K. Miyamoto, T. Tsuchiya, K. Tomoo and H. Tsujibo

Synopsis: V. vulnificus VatD was expressed, purified and crystallized. Diffraction data were collected to 2.60 Å resolution for apo VatD and to 2.03 Å resolution for a VatD-desferrioxamine B-Fe3+ complex.

Accepted 12 June 2015

Crystallographic analysis of archaeal ribosomal protein L11

I. Mitroshin, M. Garber and A. Gabdulkhakov

Synopsis: Crystallization and X-ray crystallographic studies of the archaeal ribosomal protein L11 from M. jannaschii are reported.

Accepted 12 June 2015

In vivo protein crystallization in combination with highly brilliant radiation sources offers novel opportunities for the structural analysis of post-translationally modified eukaryotic proteins

M. Duszenko, L. Redecke, C. N. Mudogo, B. P. Sommer, S. Mogk, D. Oberthuer and C. Betzel

Synopsis: In vivo formation of protein crystals and their isolation and structural analysis by novel highly brilliant XFEL and synchrotron-radiation sources is described.

Accepted 11 June 2015

Crystallization, neutron data collection, initial structure refinement and analysis of a xyloglucan heptamer bound to an engineered carbohydrate-binding module from xylanase

M. Ohlin, L. von Schantz, T. E. Schrader, A. Ostermann, D. Logan and S. Z. Fisher

Synopsis: The details of the preparation of a large crystal of X-2 L110F, a mutated carbohydrate-binding module of a bacterial xylanase, in complex with a branched xyloglucan oligosaccharide ligand are described. Details include crystallization, H/D exchange, room-temperature X-ray and neutron data collection, initial joint X-ray/neutron refinement using phenix.refine and initial structure analysis.

Accepted 9 June 2015

Crystallographic analysis of RsmA, a ribosomal RNA small subunit methyltransferase A from Staphylococcus aureus

Y. Liu, Y. Zhu, M. Teng and X. Li

Synopsis: The crystallization and X-ray diffraction analysis of RsmA from S. aureus are reported.

Accepted 9 June 2015

Structure of GTP-specific succinyl-CoA synthetase in complex with CoA

J. Huang, M. Malhi, J. Deneke and M. E. Fraser

Synopsis: CoA binds in an extended conformation to the amino-terminal domain of the [alpha]-subunit of GTP-specific succinyl-CoA synthetase.

Accepted 8 June 2015

Structure of BbKI, a di­sulfide-free plasma kallikrein inhibitor

D. Zhou, D. Hansen, I. G. Shabalin, A. Gustchina, D. F. Vieira, M. V. de Brito, A. P. U. Araújo, M. L. V. Oliva and A. Wlodawer

Synopsis: The crystal structure of a serine protease inhibitor from B. bauhinioides was determined at 1.4 Å resolution and compared with the structures of other Kunitz-type inhibitors, including the closely related dual-specificity inhibitor BbCI.

Accepted 14 April 2015

Crystallization and preliminary X-ray crystallographic analysis of a nonstructural protein 15 mutant from Human coronavirus 229E

T. Huo and X. Liu

Synopsis: This article describes preliminary crystallographic data for the I26A/N52A mutant of nonstructural protein 15 from Human coronavirus 229E.

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