forthcoming articles in Acta Crystallographica Section F

The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.

This list will generally be short, as papers in this journal are published online as soon as proofs are returned.

See also Forthcoming articles in all IUCr journals.

Accepted 1 July 2015

Structure of RizA, an l-amino acid ligase from Bacillus subtilis

W. Kagawa, T. Arai, S. Ishikura, K. Kino and H. Kurumizaka

Synopsis: The structure of the l-amino acid ligase RizA was determined, at 2.8 Å resolution.

Accepted 1 July 2015

Formulation screening by differential scanning fluorimetry: how often does it work?

M. Ristic, N. Rosa, S. A. Seabrook and J. Newman

Synopsis: Over 250 proteins have been subjected to a standard dye-based thermal melt analysis, and the results suggest that at least a third of soluble protein samples can be made significantly more stable by changing the composition of their formulation.

Accepted 1 July 2015

Preliminary X-ray crystallographic studies of the middle part of the human SYCP1 coiled-coil domain

H. H. Park

Synopsis: The middle of the syneptonemal complex protein 1 (SYCP1), which is composed of a coiled-coil domain, was purified and crystallized. The crystals were found to belong to space group I4 with unit-cell parameters of a = 41.95, b = 41.95, and c = 318.78 Å. The crystals were obtained at 293 K and diffracted to a resolution of 3.0 Å.

Accepted 30 June 2015

Crystallization and crystallographic studies of the structured core domain of Knr4 from Saccharomyces cerevisiae

S. Julien, P. Tondl, F. Durand, A. Dagkessamanskaia, H. van Tilbeurgh, J.-M. François, L. Mourey, D. Zerbib, H. Martin-Yken and L. Maveyraud

Synopsis: The potentially structured core domain of the intrinsically disordered protein (IDP) Knr4, residues 80-340, was expressed and crystallized, using native and selenomethionyl-labelled proteins.

Accepted 30 June 2015

Structure of the fusion protein 14-3-3[zeta]-LKB1 provides insight into a novel ligand-binding mode of 14-3-3

S. Ding, R. Zhou and Y. Zhu

Synopsis: The crystal structure of fusion protein 14-3-3[zeta]-LKB1 provides a novel potential binding mode of 14-3-3 with its ligands.

Accepted 29 June 2015

High-resolution crystal structure of cAMP-dependent protein kinase (PKA) from Cricetulus griseus

D. Kudlinzki, V. L. Linhard, K. Saxena, S. Sreeramulu, S. Gande, U. Schieborr, M. Dreyer and H. Schwalbe

Synopsis: High resolution crystal structure of cAMP-dependent Protein Kinase (PKA)

Accepted 26 June 2015

Structure of GUN4 from Chlamydomonas reinhardtii

S. Tarahi Tabrizi, D. B. Langley, S. J. Harrop, A. P. Duff and R. D. Willows

Synopsis: Structure of a eukaryotic GUN4 compared with the prokaryotic GUN4 structures.

Accepted 24 June 2015

Structural analysis of Clostridium aceto­butylicum ATCC 824 glycoside hydrolase from CAZy family GH105

K. L. Germane, M. D. Servinsky, E. S. Gerlach, C. J. Sund and M. M. Hurley

Synopsis: The crystal structure of Clostridium acetobutylicum ATCC 824 gene CA_C0359 protein product is structurally similar to YteR, an unsaturated rhamnogalacturonyl hydrolase from Bacillus subtilis str 168. Substrate modeling and electrostatic studies of the active site of the structure of CA_C0359 suggests the protein can now be considered part of the CAZy glycoside hydrolase family 105.

Accepted 22 June 2015

Crystallization and preliminary X-ray crystallographic analysis of branching enzymes from Cyanothece sp. ATCC 51142

M. Hayashi, R. Suzuki, C. Colleoni, S. G. Ball, N. Fujita and E. Suzuki

Synopsis: Biochemical characterization and preliminary X-ray crystallographic analysis of branching enzymes from the cyanobacterium Cyanothece sp. ATCC 51142 are reported.

Accepted 22 June 2015

Structure of human roquin-2 and its complex with constitutive decay element RNA

S. Sakurai, U. Ohto and T. Shimizu

Synopsis: Roquin mediates mRNA degradation by recognizing the constitutive decay element. We determined the crystal structures of ROQ domain of Roquin-2 in the ligand-free and RNA bound forms, where roquin-2 recognized RNA forming stem-loop structure.

Accepted 18 June 2015

Expression, purification, crystallization and X-ray crystallographic analysis of the periplasmic binding protein VatD from Vibrio vulnificus M2799

N. Miyano, T. Igarashi, H. Kawano, K. Miyamoto, T. Tsuchiya, K. Tomoo and H. Tsujibo

Synopsis: V. vulnificus VatD was expressed, purified and crystallized. Diffraction data were collected to 2.60 Å resolution for apo VatD and to 2.03 Å resolution for the VatD-desferrioxamine B-Fe3+ complex.

Accepted 12 June 2015

Crystallization and crystallographic analysis of an archaeal ribosomal protein L11

I. Mitroshin, M. Garber and A. Gabdulkhakov

Synopsis: Crystallization and X-ray crystallographic studies of the archaeal ribosomal protein L11 from M. jannaschii are reported.

Accepted 12 June 2015

in vivo protein crystallization in combination with highly brilliant radiation sources offers novel opportunities for structural analysis of post-translationally modified eukaryotic proteins

M. Duszenko, L. Redecke, C. N. Mudogo, B. P. Sommer, S. Mogk, D. Oberthuer and C. Betzel

Synopsis: in vivo protein crystal formation, isolated and structural analysis by novel highly brilliant XFEL and synchrotron radiation sources.

Accepted 11 June 2015

Crystallization, neutron data collection, initial structure refinement and analysis of a xyloglucan heptamer bound to an engineered carbohydrate-binding module from xylanase

M. Ohlin, L. von Schantz, T. E. Schrader, A. Ostermann, D. Logan and S. Z. Fisher

Synopsis: The details of large crystal preparation of X-2 L110F, a mutated carbohydrate-binding module (CBM) of a bacterial xylanase, in complex with a branched xyloglucan oligosaccharide ligand are described. Details include crystallization, H/D exchange, room-temperature X-ray and neutron data collection, initial joint X-ray/neutron refinement using phenix.refine and initial structure analysis. It is shown that it is possible to grow large crystals from only 7 mg starting material and to obtain high-resolution neutron data in ~10 d. This is the first neutron structure of a CBM and detailed analysis will add to the growing understanding of protein-carbohydrate interactions at a molecular level.

Accepted 9 June 2015

Crystallographic analysis of RsmA, a ribosomal RNA small subunit methyltransferase A from Staphylococcus aureus

Y. Liu, Y. Zhu, M. Teng and X. Li

Synopsis: The crystallization and X-ray diffraction analysis of RsmA from S. aureus are reported.

Accepted 9 June 2015

Structure of GTP-specific succinyl-CoA synthetase in complex with CoA

J. Huang, M. Malhi, J. Deneke and M. E. Fraser

Synopsis: CoA binds in an extended conformation to the amino-terminal domain of the [alpha]-subunit of GTP-specific succinyl-CoA synthetase.

Accepted 8 June 2015

Overproduction, crystallization and X-ray diffraction data analysis of ectoine synthase from the cold-adapted marine bacterium Sphingopyxis alaskensis

S. Kobus, N. Widderich, A. Hoeppner, E. Bremer and S. H. J. Smits

Synopsis: Ectoine synthase from S. alaskensis, which catalyzes the production of the stress protectant ectoine, was crystallized, yielding crystals that diffracted to 1.0 Å resolution.

Accepted 8 June 2015

Crystallographic studies of SarV, a global regulator from Staphylococcus aureus

Y. Song, F. Zhang, X. Li, J. Zang and X. Zhang

Synopsis: The cloning, expression and crystallographic studies of SarV from S. aureus are reported.

Accepted 8 June 2015

Crystallographic analysis of NosA, which catalyzes terminal amide formation in the biosynthesis of nosiheptide

Y. Wang, S. Liu, P. Yao, Y. Yu, Y. Zhang, W. Lan, C. Wang, J. Ding, W. Liu and C. Cao

Synopsis: The crystallization of the enzyme NosA, which catalyzes nosiheptide maturation, is reported.

Accepted 8 June 2015

Structure of BbKI, a di­sulfide-free plasma kallikrein inhibitor

D. Zhou, D. Hansen, I. G. Shabalin, A. Gustchina, D. F. Vieira, M. V. de Brito, A. P. U. Araújo, M. L. V. Oliva and A. Wlodawer

Synopsis: The crystal structure of a serine protease inhibitor from B. bauhinioides was determined at 1.4 Å resolution and compared with the structures of other Kunitz-type inhibitors, including the closely related dual-specificity inhibitor BbCI.

Accepted 3 June 2015

Crystallographic analysis of FAD-dependent glucose de­hydrogenase

H. Komori, K. Inaka, N. Furubayashi, M. Honda and Y. Higuchi

Synopsis: The crystallization and crystallographic study of an FAD-dependent glucose dehydrogenase from A. terreus are reported.

Accepted 2 June 2015

Structures of Pseudomonas aeruginosa [beta]-keto­acyl-(acyl-carrier-protein) synthase II (FabF) and a C164Q mutant provide templates for antibacterial drug discovery and identify a buried potassium ion and a ligand-binding site that is an artefact of the crystal form

B. Baum, L. S. M. Lecker, M. Zoltner, E. Jaenicke, R. Schnell, W. N. Hunter and R. Brenk

Synopsis: Three crystal structures of recombinant P. aeruginosa FabF are reported: the apoenzyme, an active-site mutant and a complex with a fragment of a natural product inhibitor. The characterization provides reagents and new information to support antibacterial drug discovery.

Accepted 1 June 2015

Crystallographic studies of aspartate racemase from Lactobacillus sakei NBRC 15893

T. Fujii, T. Yamauchi, M. Ishiyama, Y. Gogami, T. Oikawa and Y. Hata

Synopsis: The aspartate racemase from L. sakei NBRC 15893 has been crystallized by the sitting-drop vapour-diffusion method. The crystal diffracted to 2.6 Å resolution.

Accepted 31 May 2015

Structure of [alpha]-carbonic anhydrase from the human pathogen Helicobacter pylori

M. E. Compostella, P. Berto, F. Vallese and G. Zanotti

Synopsis: The crystal structure of [alpha]-carbonic anhydrase, an enzyme present in the periplasm of H. pylori and a potential drug target, is described.

Accepted 30 May 2015

The soluble Y115E-Y117E variant of human glutaminyl cyclase is a valid target for X-ray and NMR screening of inhibitors against Alzheimer disease

F. DiPisa, C. Pozzi, M. Benvenuti, M. Andreini, G. Marconi and S. Mangani

Synopsis: An easy to produce, soluble variant of human glutaminyl cyclase has been validated for NMR and X-ray crystallographic screening of candidate inhibitors for lead development against neurodegenerative disorders.

Accepted 26 May 2015

Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries)

D. Sakthivel, D. Littler, A. Shahine, S. Troy, M. Johnson, J. Rossjohn, D. Piedrafita and T. Beddoe

Synopsis: The cloning, expression, purification, crystallization and crystallographic studies of ruminant-specific galectin-11 are described.

Accepted 26 May 2015

Crystallographic analysis of the N-terminal domain of Middle East respiratory syndrome coronavirus nucleocapsid protein

Y.-S. Wang, C. Chang and M.-H. Hou

Synopsis: Crystals of the N-terminal domain of the nucleocapsid protein from Middle East respiratory syndrome coronavirus that diffracted X-rays to a resolution of at least 2.63 Å are described.

Accepted 26 May 2015

Structure of the human MLH1 N-terminus: implications for predisposition to Lynch syndrome

H. Wu, H. Zeng, R. Lam, W. Tempel, I. D. Kerr and J. Min

Synopsis: The crystal structure of the human MLH1 N-terminus is reported at 2.30 Å resolution. The overall structure is described along with an analysis of two clinically important mutations.

Accepted 25 May 2015

Phormidium phycoerythrin forms hexamers in crystals: a crystallographic study

R. R. Sonani, M. Sharma, G. D. Gupta, V. Kumar and D. Madamwar

Synopsis: Crystallographic analysis of marine phycoerythrin is described, showing distinct sequence features.

Accepted 22 May 2015

Molecular cloning, overexpression, purification and crystallographic analysis of a GH43 [beta]-xylosidase from Bacillus licheniformis

J. A. Diogo, L. M. Zanphorlin, H. H. Sato, M. T. Murakami and R. Ruller

Synopsis: A GH43 [beta]-xylosidase from the bacterium B. licheniformis was cloned, overexpressed in E. coli BL21(DE3) cells, purified and crystallized. The crystals belonged to the monoclinic space group C2 and diffracted to 2.49 Å resolution.

Accepted 22 May 2015

Purification and crystallographic analysis of a FAD-dependent halogenase from Streptomyces sp. JCM9888

Y. Zhao, B. Yan, T. Yang, J. Jiang, H. Wei and X. Zhu

Synopsis: The halogenase HalY, which is highly similar to PltA involved in pyoluteorin biosynthesis, was expressed in E. coli and purified as a monomer. X-ray diffraction data were collected to a resolution of 1.7 Å from a crystal belonging to space group P21.

Accepted 21 May 2015

Structure of the response regulator ChrA in the haem-sensing two-component system of Corynebacterium diphtheriae

A. Doi, H. Nakamura, Y. Shiro and H. Sugimoto

Synopsis: The structure of the full-length response regulator ChrA in an unphosphorylated state reveals a unique linker conformation and an interdomain reorientation.

Accepted 20 May 2015

Purification and crystallographic studies of a putative carbohydrate-binding module from the Ruminococcus flavefaciens FD-1 endoglucanase Cel5A

A. J. Pires, T. Ribeiro, A. Thompson, I. Venditto, V. O. Fernandes, P. Bule, H. Santos, V. D. Alves, V. Pires, L. M. A. Ferreira, C. M. G. A. Fontes and S. Najmudin

Synopsis: A putative carbohydrate-binding module from a modular family 5 glycoside hydrolase from R. flavefaciens FD-1 was purified and crystallized, and data were collected from cacodylate-derivative crystals to 1.02 and 1.29 Å resolution.

Accepted 19 May 2015

Crystallographic studies of the extracytoplasmic function [sigma] factor [sigma]J from Mycobacterium tuberculosis

K. Goutam, A. K. Gupta and B. Gopal

Synopsis: Crystallographic X-ray diffraction studies of M. tuberculosis [sigma]J are reported. M. tuberculosis [sigma]J is an extracytoplasmic function [sigma] factor that governs the cellular response to oxidative stress.

Accepted 18 May 2015

Structure of recombinant prolidase from Thermococcus sibiricus in space group P21221

V. Timofeev, E. Slutskaya, M. Gorbacheva, K. Boyko, T. Rakitina, D. Korzhenevskiy, A. Lipkin and V. Popov

Synopsis: The crystal structure of recombinant prolidase from T. sibiricus was determined by X-ray diffraction to a resolution of 2.6 Å and deposited in the Protein Data Bank.

Accepted 15 May 2015

Structure of human collapsin response mediator protein 1: a possible role of its C-terminal tail

S.-H. Liu, S.-F. Huang, Y.-L. Hsu, S.-H. Pan, Y.-J. Chen and Y.-H. Lin

Synopsis: The crystal structure of a functional C-terminal domain of human collapsin response mediator protein 1 has been determined at 3 Å resolution.

Accepted 14 April 2015

Crystallization and preliminary X-ray crystallographic analysis of a nonstructural protein 15 mutant from Human coronavirus 229E

T. Huo and X. Liu

Synopsis: This article describes preliminary crystallographic data for the I26A/N52A mutant of nonstructural protein 15 from Human coronavirus 229E.

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