forthcoming articles in Acta Crystallographica Section F

The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.

This list will generally be short, as papers in this journal are published online as soon as proofs are returned.

See also Forthcoming articles in all IUCr journals.


Accepted 17 April 2014

Crystallization and preliminary crystallographic analysis of human muscle phosphofructokinase, the main regulator of glycolysis

M. Kloos, A. Brüser, J. Kirchberger, T. Schöneberg and N. Sträter

Synopsis: Wild type human muscle Pfk was crystallized in space group P6222. Molecular replacement with the rabbit muscle homolog shows that a dimer of the physiological tetramer is present in the crystals.


Accepted 16 April 2014

Expression, purification and preliminary crystallographic analysis of Mycobacterium tuberculosis CysQ, a phosphatase involved in sulfur metabolism

A. Erickson, R. Sarsam and A. J. Fisher


Accepted 16 April 2014

Applications of the second virial coefficient: protein crystallization and solubility

W. W. Wilson and L. J. DeLucas


Accepted 15 April 2014

Crystallization and preliminary crystallographic studies of PotA, a membrane-associated ATPase of the spermidine-preferential uptake system in Thermotoga maritima

S. Sugiyama, K. Kashiwagi, K. Kakinouchi, H. Tomitori, K. Kanai, M. Murata, H. Adachi, H. Matsumura, K. Takano, S. Murakami, T. Inoue, Y. Mori and K. Igarashi

Synopsis: Native and SeMet-labelled PotA proteins from Thermotoga maritima were expressed, purified and crystallized, and X-ray diffraction data were collected to 2.7 Å resolution.


Accepted 15 April 2014

Expression, purification, and crystallization of a fungal type III polyketide synthase that produces the csypyrones

D. Yang, T. Mori, T. Matsui, M. Hashimoto, H. Morita, I. Fujii and I. Abe

Synopsis: CsyB from Aspergillus oryzae has been overexpressed in E. coli, purified, and crystallized. Diffraction data have been collected to a resolution of 1.71 Å.


Accepted 14 April 2014

Crystallization and preliminary X-ray crystallographic analysis of Streptomyces coelicolor A3(2) carboxyl-terminal region 4 of SigR

K. Y. Kim, S. Kim, J. K. Park, H. Song and S. Park

Synopsis: Carboxyl-terminal region 4 of SigR ([sigma]R4) from Streptomyces coelicolor A3(2) has been crystallized (P43212 with unit-cell parameters of a = b = 42.14 Å, c = 102.02 Å). Diffraction data have been collected to 2.60 Å resolution using synchrotron radiation.


Accepted 14 April 2014

Overproduction, purification, crystallization and preliminary X-ray characterization of the family 46 carbohydrate-binding module (CBM46) of endo-[beta]-1,4-glucanase B (CelB) from Bacillus halodurans

I. Venditto, H. Santos, L. M. A. Ferreira, K. Sakka, C. M. G. A. Fontes and S. Najmudin

Synopsis: A family 46 carbohydrate-binding module (BhCBM46) from the Bacillus halodurans endo-[beta]-1,4-glucanase B, CelB, was purified, crystallized and data were collected for both the native and seleno-methionine derivatives to 2.46 Å and 2.3 Å resolution, respectively.


Accepted 14 April 2014

Crystallization and preliminary crystallographic analysis of defective pollen wall (DPW) protein from Oryza sativa

W. Wang, Y. Ma, Y. Suo, L. Yan, D. Zhang and C. Miao

Synopsis: Mutations of defective pollen wall (DPW) cause abnormalities in the development of anthers and pollen grains of Oryza sativa. In this study, we cloned and expressed DPW[Delta]80 protein in Escherichia coli. Purified DPW[Delta]80 in complex with its co-factor NADPH formed crystals. Diffraction data were collected to 3.4 Å resolution.


Accepted 14 April 2014

Crystallization and preliminary X-ray diffraction analysis of AntE, a crotonyl-CoA carboxylase/reductase from Streptomyces sp. NRRL 2288

L. Zhang, J. Chen, T. Mori, Y. Yan, W. Liu and I. Abe

Synopsis: AntE, a crotonyl-CoA carboxylase/reductase involved in the biosynthesis of antimycin A from Streptomyces sp. NRRL 2288, has been overexpressed in E. coli, purified, and crystallized. Diffraction data have been collected to a resolution of 2.29 Å.


Accepted 13 April 2014

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of a ferredoxin/flavodoxin-NADP(H) oxidoreductase (Bc0385) from Bacillus cereus

S. Skråmo, H.-P. Hersleth, M. Hammerstad, K. K. Andersson and Å. K. Røhr

Synopsis: A ferredoxin/flavodoxin-NADP(H) oxidoreductase (FNR) from Bacillus cereus (Bc0385) belonging to the thioredoxin reductase-like class of FNRs, has been cloned, overexpressed, purified and crystallized. Diffraction data has been collected to 2.5 Å.


Accepted 12 April 2014

GlmU (N-acetylglucosamine-1-phosphate uridyltransferase) bound to three magnesium ions and ATP at the active site

N. Vithani, V. Bais and B. Prakash


Accepted 11 April 2014

Expression, purification, crystallization and >preliminary X-ray diffraction analysis of the C-terminal NHL domain of human TRIM2

X. Guan, J. Li, X. Lü, Y. Dong, W. Chen and X. Li


Accepted 10 April 2014

Crystallization and preliminary X-ray analysis of the ergothioneine-biosynthetic methyltransferase EgtD

A. Vit, L. Misson, W. Blankenfeldt and F. P. Seebeck

Synopsis: The SAM-dependent methyltransferase EgtD, an enzyme involved in ergothioneine biosynthesis, has been crystallized and native data have been collected as well as anomalous diffraction data at the K edge of a crystal of seleno-L-methionine-labelled protein.


Accepted 8 April 2014

Structure of tyrosine aminotransferase from Leishmania infantum

M. A. Moreno, A. Abramov, J. Abendroth, A. Alonso, S. Zhang, P. J. Alcolea, T. Edwards, D. Lorimer, P. J. Myler and V. Larraga


Accepted 7 April 2014

The purification, crystallization and preliminary X-ray diffraction analysis of two isoforms of meso-diaminopimelate decarboxylase from Arabidopsis thaliana

M. R. Oliver, J. M. Crowther, M. M. Leeman, S. A. Kessans, R. A. North, K. A. Donovan, M. D. W. Griffin, H. Suzuki, A. O. Hudson, M. Kasanmascheff and R. C. J. Dobson

Synopsis: Diaminopimelate decarboxylase catalyses the last step in lysine biosynthesis: the decarboxylation of meso-diaminopimelate to form the essential amino acid S-lysine. Here, the crystallization of the two putative diaminopimelate decarboxylase isoforms from Arabidopsis thaliana, DapDc1 and DapDc2, are reported; the crystals diffracted to beyond 2.00 and 2.27 Å resolution, respectively.


Accepted 7 April 2014

Expression, purification and crystallization of MnSOD from Arabidopsis thaliana

A. T. Marques, S. P. Santos, M. G. Rosa, M. A. A. Rodrigues, I. A. Abreu, C. Frazão and C. V. Romão

Synopsis: Manganese superoxide dismutase (MnSOD) from A. thaliana was expressed, purified and crystallized. An X-ray diffraction data set was collected to a resolution of 1.95 Å.


Accepted 4 April 2014

Purification, crystallization and phase determination of the DR1998 haem b catalase from Deinococcus radiodurans

P. T. Borges, C. S. Miranda, S. P. Santos, J. N. Carita, C. Frazão and C. V. Romão

Synopsis: Crystals of the DR1998 catalase from the radiation-resistant bacterium D. radiodurans have been produced. The purification of the protein from the native source, its crystallization and preliminary X-ray crystallographic analysis are reported.


Accepted 3 April 2014

Purification, crystallization and preliminary X-ray characterization of the third ScaB cohesin in complex with an ScaA X-dockerin from Acetivibrio cellulolyticus

K. Cameron, V. D. Alves, P. Bule, L. M. A. Ferreira, C. M. G. A. Fontes and S. Najmudin

Synopsis: A ScaA X-dockerin derivative with an engineered restricted single binding mode complexed with the third ScaB cohesin from A. cellulolyticus has been crystallized and data were collected to a resolution of 2.41 Å.


Accepted 2 April 2014

Structure of a His170Tyr mutant of thermostable pNPPase from Geobacillus stearothermophilus

T. Shen, Z. Guo and C. Ji


Accepted 1 April 2014

Crystallization and preliminary X-ray crystallographic analysis of a bacterial Asn-transamidosome

T. Suzuki, K. Yamashita, Y. Tanaka, I. Tanaka and M. Yao

Synopsis: A bacterial Asn-transamidosome composed of two GatCABs, one AspRS dimer and two tRNAAsns from P. aeruginosa was crystallized and the crystal diffracted to 3.73 Å resolution.


Accepted 26 March 2014

Purification, crystallization and preliminary X-ray analysis of a putative nucleotide phosphohydrolase, YpgQ, from Bacillus subtilis

Y. J. Jeon, W. S. Song and S. Yoon

Synopsis: A putative nucleotide phosphohydrolase, YpgQ, from B. subtilis was expressed, purified and crystallized. The YpgQ crystal diffracted X-rays to a resolution of 2.3 Å.


Accepted 19 March 2014

Crystallization and preliminary X-ray diffraction analysis of proximal thread matrix protein 1 (PTMP1) from Mytilus galloprovincialis

M. H. Suhre, T. Scheibel, C. Steegborn and M. Gertz

Synopsis: The full-length proximal thread matrix protein 1 (PTMP1) from M. galloprovincialis was recombinantly produced, purified and crystallized. Diffraction analysis of the obtained crystals showed that they belonged to space group P21 and diffracted to 1.95 Å resolution.




































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