The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.
This list will generally be short, as papers in this journal are published online as soon as proofs are returned.
See also Forthcoming articles in all IUCr journals.
Synopsis: Six subunits of human prefoldin were overexpressed in E. coli. Prefoldin was reconstituted, purified and crystallized. X-ray diffraction data were collected at 4.7 Å resolution.
Synopsis: The structure of the SPRY domain of the human RNA helicase DDX1 was determined at 2.0 Å resolution. The SPRY domain provides a putative protein-protein interaction platform within DDX1 that differs in its structure and conserved regions from other SPRY domains.
Synopsis: The crystal structure of the dimerization domain of CEACAM7, a human cell adhesion protein, was determined at 1.47 Å resolution and the Kdimerization was measured by analytical ultracentrifugation.
Synopsis: High-resolution structure analyses of the N-glycosidase ribosome-inactivating protein from seeds of M. charantia shows that the enzyme preferentially cleaves nicotinamide from the oxidized form of the coenzyme NADP.
Synopsis: The actin-polymerizing protein Arp2/3 complex was crystallized in two new space groups. The new electron-density maps from these new crystal forms reveal some details that may help to elucidate how nucleation-promoting factors bind to the complex.
Synopsis: The crystal structure of c4763, a uropathogenic E. coli-specific protein, at a resolution of 1.45 Å reveals that it is a structural homologue of allophanate hydrolase, which is involved in the urea cycle. These results suggest that c4763 might be involved in urea utilization, which is necessary for bacterial survival in the urinary tract.
Synopsis: The crystallization of human kallistatin in the relaxed conformation is reported.
Synopsis: The crystallization of the novel virulence factors SghA and SghR is reported.
Synopsis: The structure of the L-amino-acid ligase RizA was determined at 2.8 Å resolution.
Synopsis: Over 250 proteins have been subjected to a standard dye-based thermal melt analysis, and the results suggest that at least a third of soluble protein samples can be made significantly more stable by changing the composition of their formulation.
Synopsis: The middle of syneptonemal complex protein 1, which is composed of a coiled-coil domain, was purified and crystallized. The crystals were found to belong to space group I4, with unit-cell parameters a = 41.95, b = 41.95, c = 318.78 Å. The crystals were obtained at 293 K and diffracted to a resolution of 3.0 Å.
Synopsis: The potentially structured core domain of the intrinsically disordered protein Knr4, residues 80-340, was expressed and crystallized using native and selenomethionine-labelled proteins.
Synopsis: The crystal structure of a 14-3-3-LKB1 fusion protein provides a novel potential binding mode of 14-3-3 to its ligands.
Synopsis: The high-resolution crystal structure of a cAMP-dependent protein kinase is reported.
Synopsis: An engineered human carbonic anhydrase IX (CA IX) mimic was co-crystallized with the disaccharide sucrose. Its binding provides a novel `interaction' site that can be used to design targeted inhibitors of CA IX, a key marker of hypoxic tumors.
Synopsis: The structure of a eukaryotic GUN4 is compared with prokaryotic GUN4 structures.
Synopsis: The crystal structure of the protein product of the C. acetobutylicum ATCC 824 gene CA_C0359 is structurally similar to YteR, an unsaturated rhamnogalacturonyl hydrolase from B. subtilis strain 168. Substrate modeling and electrostatic studies of the active site of the structure of CA_C0359 suggests that the protein can now be considered to be part of CAZy glycoside hydrolase family 105.
Synopsis: The biochemical characterization and X-ray crystallographic analysis of branching enzymes from the cyanobacterium Cyanothece sp. ATCC 51142 are reported.
Synopsis: Roquin mediates mRNA degradation by recognizing the constitutive-decay element. Crystal structures of the ROQ domain of Roquin-2 were determined in ligand-free and RNA-bound forms, in which Roquin-2 recognized RNA forming a stem-loop structure.
Synopsis: V. vulnificus VatD was expressed, purified and crystallized. Diffraction data were collected to 2.60 Å resolution for apo VatD and to 2.03 Å resolution for a VatD-desferrioxamine B-Fe3+ complex.
Synopsis: Crystallization and X-ray crystallographic studies of the archaeal ribosomal protein L11 from M. jannaschii are reported.
Synopsis: In vivo formation of protein crystals and their isolation and structural analysis by novel highly brilliant XFEL and synchrotron-radiation sources is described.
Synopsis: The details of the preparation of a large crystal of X-2 L110F, a mutated carbohydrate-binding module of a bacterial xylanase, in complex with a branched xyloglucan oligosaccharide ligand are described. Details include crystallization, H/D exchange, room-temperature X-ray and neutron data collection, initial joint X-ray/neutron refinement using phenix.refine and initial structure analysis.
Synopsis: The crystallization and X-ray diffraction analysis of RsmA from S. aureus are reported.
Synopsis: CoA binds in an extended conformation to the amino-terminal domain of the -subunit of GTP-specific succinyl-CoA synthetase.
Synopsis: The crystal structure of a serine protease inhibitor from B. bauhinioides was determined at 1.4 Å resolution and compared with the structures of other Kunitz-type inhibitors, including the closely related dual-specificity inhibitor BbCI.
Synopsis: This article describes preliminary crystallographic data for the I26A/N52A mutant of nonstructural protein 15 from Human coronavirus 229E.
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