forthcoming articles in Acta Crystallographica Section F

The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.

This list will generally be short, as papers in this journal are published online as soon as proofs are returned.

See also Forthcoming articles in all IUCr journals.


Accepted 25 April 2015

Protein production, crystallization and preliminary crystallographic analysis of the N-terminal four immunoglobulin domains of Down syndrome cell adhesion molecule 1

L. Cheng, S.-A. Li, Y. Yu and Q. Chen

Synopsis: X-ray data of eight different Dscam1 Ig1-4 isoforms were collected to 1.9-4.0 Å, respectively. These structures will provide the opportunity to do extensive structural comparison of different Dscam1 isoforms and shed insights into its specificity.


Accepted 21 April 2015

Purification, crystallization and preliminary X-ray diffraction analysis of 3-keto­acyl-CoA thiolase A1887 from Ralstonia eutropha H16

J. Kim and K.-J. Kim

Synopsis: 3-Ketoacyl-CoA thiolase from R. eutropha (ReH16_A1887), an enzyme involved in [beta]-oxidation degradative pathways, has been crystallized, and X-ray diffraction data have been collected from the crystal and analyzed.


Accepted 21 April 2015

Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of NAD synthetase from methicillin resistant Staphylococcus aureus

G. K. Arbade and S. K. Srivastava

Synopsis: Nicotinamide adenine dinucleotide synthetase (NAD synthetase) from methicillin-resistant S. aureus was cloned, purified, crystallized and X-ray diffraction data were collected to a resolution of 2.0 Å.


Accepted 21 April 2015

Crystallization and preliminary X-ray diffraction analysis of the CSU pili CsuC-CsuA/B chaperone-major subunit pre-assembly complex from Acinetobacter baumannii

N. Pakharukova, M. Tuittila, S. Paavilainen and A. Zavialov

Synopsis: The Acinetobacter baumannii biofilm-forming CSU fimbriae are assembled via the archaic CsuC/CsuD chaperone/usher pathway. To determine the structural basis for the biogenesis of CSU fimbriae, native and SeMet-substituted CsuC-CsuA/B chaperone-major subunit pre-assembly complexes were produced in Escherichia coli, purified and crystallized. Initial phases were derived from single anomalous diffraction (SAD) experiment using the SeMet derivative.


Accepted 16 April 2015

Crystallization and preliminary crystallographic analysis of latent, active and recombinantly expressed aurone synthase, a polyphenol oxidase, from Coreopsis grandiflora

C. Molitor, S. G. Mauracher and A. Rompel

Synopsis: Latent and active aurone synthase purified from petals of C. grandiflora (cgAUS1) were crystallized. The crystal quality of recombinantly expressed latent cgAUS1 was significantly improved by co-crystallization with the polyoxotungstate Na6[TeW6O24] within the liquid-liquid phase-separation zone.


Accepted 16 April 2015

Structure of the Mre11 catalytic domain from Chaetomium thermophilum

F. U. Seifert, K. Lammens and K.-P. Hopfner

Synopsis: Crystal structure of the Mre11 nuclease dimer from the thermophilic eukaryote Chaetomium thermophilum. Crystals of Chaetomium thermophilum Mre11, which plays an important role in DNA double-strand break repair, diffracted up to 2.8 Å and the structure was solved by the molecular replacement technique.


Accepted 14 April 2015

Crystallization and preliminary X-ray crystallographic analysis of a nonstructural protein 15 mutant from Human coronavirus 229E

T. Huo and X. Liu

Synopsis: This article describes preliminary crystallographic data for the I26A/N52A mutant of nonstructural protein 15 from human coronavirus 229E.


Accepted 11 April 2015

Structure of the MarR family protein Rv0880 from Mycobacterium tuberculosis

Y.-R. Gao, N. Feng, T. Chen, D.-F. Li and L.-J. Bi

Synopsis: The crystal structure of the M. tuberculosis MarR family protein Rv0880 is described.


Accepted 9 April 2015

Crystallization and preliminary X-ray diffraction analysis of the CRISPR-Cas RNA-silencing Cmr complex

T. Osawa, H. Inanaga and T. Numata

Synopsis: A Cmr1-deficient functional Cmr (Cmr[Delta]1) complex composed of P. furiosus Cmr2-Cmr3, A. fulgidus Cmr4-Cmr5-Cmr6 and the 39-mer P. furiosus 7.01-crRNA was cocrystallized with single-stranded DNA complementary to the crRNA guide. X-ray diffraction data for the crystals were collected to 2.1 Å resolution using a synchrotron-radiation source.


Accepted 8 April 2015

X-ray structure of cyanide-bound bovine heart cytochrome c oxidase in the fully oxidized state at 2.0 Å resolution

N. Yano, K. Muramoto, M. Mochizuki, K. Shinzawa- Itoh, E. Yamashita, S. Yoshikawa and T. Tsukihara

Synopsis: The X-ray structure of cyanide-bound bovine heart cytochrome c oxidase in the fully oxidized state was determined at 2.0 Å resolution. This structure shows that a cyanide ion bridges the two metals in a nearly symmetric end-on fashion.


Accepted 8 April 2015

Purification, crystallization and X-ray crystallographic studies of a Bacillus cereus MepR-like transcription factor, BC0657

M. U. Cho, M. I. Kim and M. Hong

Synopsis: A MepR-like transcription factor, BC0657, from B. cereus was expressed, purified and crystallized. X-ray diffraction data for the BC0657 crystals were collected to a resolution of 2.05 Å.


Accepted 7 April 2015

Structure of Chlamydomonas reinhardtii THB1, a group 1 truncated hemoglobin with a rare histidine-lysine heme ligation

S. L. Rice, L. E. Boucher, J. L. Schlessman, M. R. Preimesberger, J. Bosch and J. T. J. Lecomte

Synopsis: The structure of THB1, a monomeric 2/2 hemoglobin involved in nitrogen metabolism in C. reinhardtii, was solved to 1.9 Å resolution. The heme is coordinated by the proximal histidine and a distal lysine.


Accepted 3 April 2015

Expression, crystallization and X-ray diffraction analysis of a complex between B7-H6, a tumor cell ligand for the natural cytotoxicity receptor NKp30, and an inhibitory antibody

X. Xu, Y. Li, L. Gauthier, Q. Chen, E. Vivier and R. A. Mariuzza

Synopsis: Crystals of a complex between human B7-H6, a tumor cell ligand for NKp30, and an inhibitory monoclonal antibody have been obtained. The crystals diffracted to 2.5 Å resolution.


Accepted 2 April 2015

Functional characterization of heat-shock protein 90 from Oryza sativa and crystal structure of its N-terminal domain

S. Raman and K. Suguna

Synopsis: Binding studies and an activity assay were performed for heat-shock protein 90 from O. sativa. The N-terminal domain of Hsp90 was crystallized and its structure was determined in complex with AMPPCP.


Accepted 31 March 2015

The structure of a contact-dependent growth-inhibition (CDI) immunity protein from Neisseria meningitidis MC58

K. Tan, P. M. Johnson, L. Stols, B. Boubion, W. Eschenfeldt, G. Babnigg, C. S. Hayes, A. Joachimiak and C. W. Goulding

Synopsis: Contact-dependent growth inhibition (CDI) is an important mechanism of inter-cellular competition between Gram-negative bacteria. The X-ray crystal structure of an immunity protein from N. meningitidis MC58 is presented together with a structural model of the toxin-immunity protein complex.


Accepted 30 March 2015

High-resolution crystal structure of the human tumour suppressor PP32A (ANP32A) leucine-rich repeat domain

S. Zamora-Caballero, L. Siauciunaite-Gaubard and J. Bravo

Synopsis: The crystal structure of the LRR domain from PP32A has been solved to 1.6 Å resolution.


Accepted 29 March 2015

Expression, purification and crystallization of a family 55 [beta]-1,3-glucanase from Chaetomium thermophilum

A. C. Papageorgiou and D. Li

Synopsis: A [beta]-1,3-glucanase from the thermophilic fungus C. thermophilum was overexpressed in P. pastoris, purified and crystallized. In situ diffraction at a synchrotron and seeding were used to obtain reproducible crystals. A complete data set to 2.0 Å resolution was collected using an in-house X-ray generator.


Accepted 28 March 2015

Purification, crystallization and preliminary X-ray diffraction analysis of the Escherichia coli common pilus chaperone EcpB

J. A. Garnett, M. Diallo and S. J. Matthews


Accepted 26 March 2015

Overexpression, purification, crystallization and preliminary X-ray diffraction of the nisin resistance protein from Streptococcus agalactiae

S. Khosa, A. Hoeppner, D. Kleinschrodt and S. Smits

Synopsis: The antibiotic-resistance protein NSR from S. agalactiae was crystallized in two different crystal forms, which diffracted X-rays to a maximum resolution of 2.2 Å.


Accepted 25 March 2015

A preliminary X-ray study of 3-de­oxy-D-manno-oct-2-ulosonic acid 8-phosphate phosphatase (YrbI) from Burkholderia pseudomallei

J. Park, D. Lee, M.-S. Kim, D. Y. Kim and D. H. Shin

Synopsis: In order to understand the structure-function relationship of 3-deoxy-D-manno-oct-2-ulosonic acid 8-phosphate phosphatase (YrbI) from B. pseudomallei, its crystallographic study has been performed. Here, the cloning, overexpression, purification, crystallization and preliminary X-ray study of this enzyme are reported.


Accepted 24 March 2015

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of CofB, the minor pilin subunit of CFA/III from human enterotoxigenic Escherichia coli

K. Kawahara, H. Oki, S. Fukakusa, T. Maruno, Y. Kobayashi, D. Motooka, T. Taniguchi, T. Honda, T. Iida, S. Nakamura and T. Ohkubo

Synopsis: The CFA/III minor pilin subunit, CofB, has been crystallized. An N-terminal hydrophobic portion of CofB was truncated to enable initial crystallization. Dehydration of preliminary crystals yielded higher resolution diffraction patterns.


Accepted 24 March 2015

Preliminary crystallographic analysis of the Arabidopsis thaliana BAG5-calmodulin protein complex

B. Cui, S. Fang, Y. Xing, Y. Shen and X. Yang

Synopsis: A. thaliana BAG5 (AtBAG5) and calmodulin were expressed and purified separately and then co-crystallized. The preliminary X-ray diffraction studies of the protein complex are reported in order to provide structural insights into the mode of interaction between AtBAG5 and calmodulin.


Accepted 24 March 2015

Crystallographic study of a novel DNA-binding domain of human HLTF involved in the template-switching pathway to avoid the replication arrest caused by DNA damage

Y. Ikegaya, K. Hara, A. Hishiki, H. Yokoyama and H. Hashimoto

Synopsis: Crystallization and diffraction studies of the HIRAN domain of human HLTF are reported.


Accepted 21 March 2015

Crystallization and preliminary X-ray diffraction analysis of the two distinct types of zebrafish [beta]2-microglobulin

Z. Chen, N. Zhang, S. Lu, M. Tariq, J. Wang and C. Xia

Synopsis: Diffraction data from crystals of zebrafish (D. rerio) [beta]2-microglobulin type I and type II (Dare-[beta]2m-I and Dare-[beta]2m-II) were collected to 1.6 and 1.9 Å resolution, respectively.


Accepted 20 March 2015

Multiple crystal forms of N,N'-di­acetylchitobiose de­acetylase from Pyrococcus furiosus

T. Nakamura, M. Niiyama, W. Hashimoto, K. Ida, M. Abe, J. Morita and K. Uegaki

Synopsis: The crystal packing of N,N'-diacetylchitobiose deacetylase from P. furiosus varied depending on the presence of cadmium and whether the native protein or its selenomethionine derivative was used. The cadmium complex was involved in the crystal contacts in Cd-containing conditions.


Accepted 12 March 2015

Structure of human dual-specificity phosphatase 7, a potential cancer drug target

G. T. Lountos, B. P. Austin, J. E. Tropea and D. S. Waugh

Synopsis: The crystal structure of the catalytic domain of human dual-specificity phosphatase 7 (C232S) is presented at 1.67 Å resolution.


Accepted 27 February 2015

Iron superoxide dismutases in eukaryotic pathogens: new insights from Apicomplexa and Trypanosoma structures

I. Q. H. Phan, D. R. Davies, N. S. Moretti, D. Shanmugam, I. Cestari, A. Anupama, J. W. Fairman, T. E. Edwards, K. Stuart, S. Schenkman and P. J. Myler

Synopsis: Superoxide dismutases (SODs) catalyze the conversion of superoxide to molecular oxygen, thus protecting organisms against toxic radicals. Iron-dependent SODs from T. cruzi, L. major and B. bovis exist as multiple isoforms with distinct structural features.


Accepted 9 February 2015

The structure of hookworm platelet inhibitor (HPI), a CAP superfamily member from Ancylostoma caninum

D. Ma, I. M. B. Francischetti, J. M. C. Ribeiro and J. F. Andersen

Synopsis: Hookworm platelet inhibitor belongs to the cysteine-rich/antigen-5/pathogenesis-related 1 (CAP) protein family. It has been linked to integrin-antagonist, antiplatelet activity found in extracts of A. caninum hookworms.




































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