forthcoming articles

The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology Communications.

See also Forthcoming articles in all IUCr journals.

Accepted 7 August 2018

Redox manipulation of the manganese metal in human manganese superoxide dismutase for neutron diffraction

>Human mitochondrial manganese superoxide dismutase (MnSOD) is a major player in combating reactive oxygen species (ROS) in the human body. We have found methods to control the redox state of the active site metal of large perdeuterated MnSOD crystals. Neutron diffraction data from these crystals was collected to study the effect of redox state on proton location. These methods can be applied to other crystal systems where information on the location of protons at specific chemical states is needed.

Accepted 3 August 2018

Crystallization of ectonucleotide phosphodiesterase/pyrophosphatase-3 and orientation of the SMB domains in the full-length ectodomain

Abstract Well diffracting crystals of the rat NPP3 ectodomain were obtained for a construct lacking the SMB domains while the structure of the full-length ectodomain reveals the orientation of the SMB domains but has a partially disordered active site structure probably due to the absence of the active site zinc ions.

Accepted 3 August 2018

Comparative structure analysis of ETSi domain of ERG3 and its complex with E74 promoter DNA sequence

X-ray structure analysis of ETSi domain of human ERG3 and its complex with E74promoter DNA sequence

Accepted 30 July 2018

Crystal structure of Arabidopsis thaliana peroxiredoxin A C119S mutant

The crystal structure of antioxidant enzyme peroxiredoxin A C119S mutant from Arabidopsis thaliana.

Accepted 24 July 2018

Mosquito-larvicidal binary toxin receptor protein (Cqm1): crystallization and X-ray crystallographic analysis

Crystallization and X-ray diffraction analysis of Cqm1 protein, known for its role as receptor for mosquito-larvicidal binary toxin, is reported. The article also highlights the use of differential scanning fluorimetry for screening small-molecule ligands that could contribute to protein crystallization.

Accepted 24 July 2018

Cryo-neutron crystal structure of left-handed Z-DNA d(CGCGCG)

Accepted 23 July 2018

Re-refinement of Plasmodium falciparum orotidine 5′-monophosphate de­carboxylase provides a clearer picture of an important malarial drug target

Accepted 21 July 2018

Staphylococcus aureus lipase: purification, kinetic characterization, crystallization and crystallographic study

Staphylococcus aureus lipase

Accepted 18 July 2018

TssA from Aeromonas hydrophila: expression, purification and crystallographic studies

Crystals of different domain constructs of the TssA subunit from A. hydrophila have been crystallized in forms suitable for X-ray analysis.

Accepted 18 July 2018

X-ray structure of alisporivir in complex with cyclophilin A at 1.5 Å resolution

The crystal structure of a non-immunosuppressive cyclic peptide inhibitor bound to its target, the human cyclophilin A.

Accepted 17 July 2018

Investigation into the binding of dyes within protein crystals

The binding of 28 dyes and stains to a dozen crystalline proteins, and to four in particular, was investigated by X-ray crystallography. Crystals of 31 different complexes were studied. Although firmly incorporated into the protein crystals in substantial amounts, the dyes are disordered and are possibly bound in a similar manner to conventional detergents.

Accepted 16 July 2018

Characterization and structure determination of a llama-derived nanobody targeting the J-base binding protein 1

A camelid single-domain antibody fragment (nanobody) is shown to have high affinity towards its recognition target, the J-base binding protein 1. The serendipitous crystallization of this nanobody alone, solution of its crystal structure and its refinement to 1.64 Å resolution are described. Ensemble refinement suggests that in the ligand-free state one of the complementarity-determining regions (CDRs) is flexible, while the other two adopt well defined conformations.

Accepted 12 July 2018

Ab initio structure solution of a proteolytic fragment using ARCIMBOLDO

The crystal structure of a proteolytic fragment of an MeaB- and MMAA-like GTPase from M. smegmatis was determined using the ab initio phasing program ARCIMBOLDO.

Accepted 10 July 2018

MBP-binding DARPins facilitate the crystallization of an MBP fusion protein

Designed ankyrin-repeat proteins (DARPins) that bind to maltose-binding protein (MBP) with high affinity can facilitate the crystallization of an MBP fusion protein. The use of MBP-specific DARPins increases the probability of obtaining crystals.

Accepted 9 July 2018

Serendipitous crystallization and structure determination of bacterioferritin from Achromobacter

This study reports the structure of bacterioferritin from Achromobacter sp. Dh1f, which was serendipitously crystallized.

Accepted 9 July 2018

MSMEG_6292, a Mycobacterium smegmatis RNA polymerase secondary channel-binding protein: purification, crystallization and X-ray diffraction analysis

The M. smegmatis Gre-factor homologue MSMEG_6292 is a RNA polymerase secondary channel-binding protein. To understand its structure and function, it was cloned, expressed, purified and crystallized, and crystallographic diffraction data were collected for both the native protein and a platinum derivative.

Accepted 9 July 2018

Structure of the Fc fragment of the NIST reference antibody RM8671

The structure of the Fc fragment of the NIST reference antibody RM8671 is described in an orthorhombic crystal form. The molecular conformation is compared with those of precedents using a CH3-based reference frame and the pronounced asymmetry is linked to packing interactions.

Accepted 6 July 2018

TssA from Burkholderia cenocepacia: expression, purification, crystallization and crystallographic analysis

Analysis of the noncrystallographic symmetry of crystals of the C-terminal domain of B. cenocepacia TssA indicates a quaternary structure of 32 subunits in D16 symmetry.

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