The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology and Crystallization Communications.
This list will generally be short, as papers in this journal are published online as soon as proofs are returned.
See also Forthcoming articles in all IUCr journals.
Synopsis: The protein ccTIM is an engineered variant of the triosephosphate isomerase (TIM) enzyme and has sequence features from both prokaryotic and eukaryotic members of the TIM protein family. Moreover, it is as active as a native protein despite sharing some biophysical characteristics with a molten globular variant. This inscrutable protein was crystallized using the microbatch method and a full X-ray diffraction data set was collected to 2.2 Å resolution using an Indian synchrotron-radiation source.
Synopsis: drFrnE, a disulfide oxidoreductase from Deinococcus radiodurans was crystallized and preliminary diffraction data obtained. Crystal belonged to space group P21221 and diffracted to 1.8 Å resolution.
Synopsis: Gliomedin has an extracellular domain, which mediates interactions between the myelinating Schwann cell and the axonal surface and plays a role in the development and maintenance of the nodes of Ranvier. We report here the purification and crystallization of this olfactomedin domain.
Synopsis: The recombinant production and structural analysis of catalytically active Type II dehydroquinase from P. aeruginosa is presented.
Synopsis: The crystal structure of the RNA chaperone, Hfq in complex with RNA is presented at 0.97 Å resolution.
Synopsis: VHH R303 binds the Listeria virulence factor InlB. Here, it is reported that crystals of R303 were obtained following in situ proteolysis with trypsin. Trypsin removed the flexible tag region of the protein, creating a homogenous protein preparation. The crystals diffracted to 1.3 Å resolution.
Synopsis: The flagellar accessory protein FlaH from M. jannaschii has been purified and crystallized. The crystal structure has been solved using the MAD method.
Synopsis: -Galactosidase from A. niger has been expressed in S. cerevisiae, purified and crystallized in its deglycosylated form. X-ray data have been collected to 1.8 Å resolution.
Synopsis: The structure of a short-chain dehydrogenase encoded within genomic islands of A. baumannii strains has been solved to 2.4 Å resolution. This classical SDR incorporates a flexible helical subdomain. The NADP-binding site and catalytic side chains are identified.
Synopsis: The cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of PdxK from P. falciparum are described.
Synopsis: The C-terminal domain of C. trachomatis CdsD was purified and crystallized. The preliminary X-ray diffraction studies are presented.
Synopsis: Strategies and procedures are surveyed for the optimization of macromolecular crystal growth conditions. The objective of optimization is to grow crystals of the greatest degree of perfection and that yield the most accurate and precise X-ray diffraction data.
Synopsis: A crystal of the full-length response regulator spr1814 of S. pneumoniae in complex with a phosphoryl analogue was obtained and diffraction data were collected to 2.0[1.9?] Å resolution.
Synopsis: The thiosulfate dehydrogenase TsdA from A. vinosum was recombinantly expressed, purified and crystallized. The crystals belonged to space group C2, with unit-cell parameters a = 79.2, b = 69.9, c = 57.9 Å, = 129.3°, and diffracted to a resolution of 1.98 Å.
Synopsis: The crystal structure of the N-terminal domain of lactoferrin-binding protein B (LbpB) from N. meningitidis is reported. Docking of the structure with lactoferrin suggests roles for LbpB in both host iron acquisition and neutralization of the toxic effects of the lactoferricin peptide.
Synopsis: All seven possible single-site lysine-to-serine mutations of ubiquitin were tested for crystallization behavior and were found to yield crystallization `hit rates' varying by two orders of magnitude. High-resolution structures of three mutants revealed that mutations can exert both promoting and permissive effects on crystallization.
Synopsis: The first crystal structure for an intracellular poly(3-hydroxybutyrate) depolymerase at 1.42 Å resolution was determined by molecular replacement using a search model with 24% sequence identity.
Synopsis: The CIDE domain of Drep2 was purified and crystallized in space group P212121, with unit-cell parameters a = 50.28, b = 88.70, c = 113.37 Å. The crystals diffracted to a resolution of 3.3 Å.
Synopsis: The SMase D from L. gaucho venom was expressed, purified, crystallized and diffraction data were collected to 2.6 Å resolution.
Synopsis: The structure of the A. fumigatus old yellow enzyme EasA was determined to 1.8 Å resolution. This enzyme catalyzes the reduction of chanoclavine-I aldehyde to dihydrochanoclavine aldehyde and works in conjunction with festuclavine synthase at the branchpoint for ergot alkaloid pathways.
Synopsis: Cyclolavandulyl diphosphate synthase, which catalyzes both the condensation of two molecules of C5 dimethylallyl diphosphate and the subsequent cyclization, has been crystallized and X-ray diffraction data have been collected and analyzed.
Synopsis: McbB, a multifunctional enzyme responsible for catalysing Pictet-Spengler cyclization, decarboxylation and oxidation reactions in the biosynthesis of -carboline, was expressed and crystallized. The crystals belonged to the monoclinic space group P21, with unit-cell parameters a = 66.06, b = 85.48, c = 106.19 Å, = 90.00, = 106.77, = 90.00°.
Synopsis: A crystal was obtained of the complex between reduced terminal oxygenase and oxidized ferredoxin components of carbazole 1,9a-dioxygenase. The crystal belonged to space group P21 and diffracted to 2.25 Å resolution.
Synopsis: Lin1840 from L. innocua has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to a resolution of 1.8 Å.
Synopsis: The Ets1-Runx1-CBF-DNA complex, a higher-order TF-DNA complex formed on the T-cell receptor gene enhancer, was crystallized.
Synopsis: The crystallization and preliminary crystallographic analysis of CrArsM, an arsenic(III) S-adenosylmethionine methyltransferase from C. reinhardtii, is described.
Synopsis: The chemolithoautotrophic nitrifying thaumarchaeon Ca. Nitrososphaera gargensis with unusual metabolic pathways was enriched by cultivation over six years. A novel /-hydrolase was identified, isolated and crystallized for further functional analysis.
Synopsis: FBPA I from E. coli was expressed, purified and crystallized. The crystals diffracted to 2.0 Å resolution and belonged to space group C2.
Synopsis: The hypothetical deaminase RPB_0146 from R. palustris HaA2 was expressed in E. coli and purified. Crystallization and preliminarily X-ray crystallographic analysis of the recombinant deaminase were performed.
Synopsis: We look to the PDB to find data to answer the question `How well do our current screens cover crystallization space?' and to try to find answers to the more general question about the most efficient strategy to employ in a crystallization campaign.
Synopsis: The cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of 7-keto-8-aminopelargonic acid (KAPA) synthase from M. smegmatis are described..
Synopsis: A controversy has existed in the literature between crystallographic and spectroscopic data on the binding mode of cyanate to carbonic anhydrase II (CA II). To settle this ambiguity, the X-ray crystal structures of the complexes of wild-type and V207I variant CA II with cyanate were redetermined to 1.7 and 1.5 Å resolution, respectively. The data clearly support that cyanate binds directly to the catalytic zinc and not as an outer sphere ligand mimicking the binding of CO2.
Synopsis: Cystathionine -lyase from multidrug-resistant A. baumannii OXA-23 (AbCBL), an enzyme involved in the methionine-metabolism pathway and a novel antibacterial drug target, was cloned, expressed, purified and crystallized. Preliminary X-ray crystallography was performed to analyse the AbCBL crystal.
Synopsis: An L-amino-acid oxidase from L. muta venom was crystallized and diffraction data were collected to 3.0 Å resolution.
Synopsis: The integral membrane lysine permease from P. aeruginosa was cloned and overexpressed in E. coli as a GFP-fusion protein. Crystals of the tag-less transporter diffracted to 7.5 Å resolution.
Synopsis: Crystals of the Pax9 paired domain bound to a DC5 enhancer DNA element that diffract anisotropically to 3.0 Å resolution are reported.
Synopsis: IL-18 and its two receptors, IL-18R and IL-18R, were purified for crystallization and biochemical analysis. IL-18 was crystallized in free, IL-18R-bound and IL-18R/IL-18R-bound states and complete X-ray data sets suitable for further structural analysis were obtained from each crystal.
Synopsis: The cryptic polo-box (CPB) domain of the polo-like kinase ZYG-1 from C. elegans was cloned, overexpressed, purified and crystallized. Anomalous X-ray diffraction data were collected to 2.54 Å resolution.
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