Journal of Applied Crystallography

Volume 41, Part 6 (December 2008)

teaching and education

J. Appl. Cryst. (2008). 41, 1161-1172 [ doi:10.1107/S0021889808032494 ]

A tutorial for learning and teaching macromolecular crystallography

A. Faust, S. Panjikar, U. Mueller, V. Parthasarathy, A. Schmidt, V. S. Lamzin and M. S. Weiss

Abstract: Five experiments have been designed to be used for teaching macromolecular crystallography. The three proteins used in this tutorial are all commercially available; they can be easily and reproducibly crystallized and mounted for diffraction data collection. For each of the five experiments the raw images and the processed data of a sample diffraction data set as well as the refined coordinates and phases are provided for teaching the steps of data processing and structure determination.

Keywords: macromolecular crystallography; protein crystallization; diffraction data collection; data processing; automated structure determination.


	Hyper-Text Markup Language (HTML) file The raw diffraction images for all five experiments of this tutorial, as well as the integrated and scaled data and the solved and refined structures are available from EMBL Hamburg.

	Hyper-Text Markup Language (HTML) file The raw diffraction images for all five experiments of this tutorial, as well as the integrated and scaled data and the solved and refined structures are available from Bessy.

	Portable Document Format (PDF) file (903.5 kbytes) [ doi:10.1107/S0021889808032494/kk5029sup1.pdf ] Tutorial 1: S-SAD on bovine insulin

	Portable Document Format (PDF) file (751.4 kbytes) [ doi:10.1107/S0021889808032494/kk5029sup2.pdf ] Tutorial 2: MAD on bromide-soaked thaumatin

	Portable Document Format (PDF) file (598.2 kbytes) [ doi:10.1107/S0021889808032494/kk5029sup3.pdf ] Tutorial 3: molecular replacement on monoclinic lysozyme

	Portable Document Format (PDF) file (301.6 kbytes) [ doi:10.1107/S0021889808032494/kk5029sup4.pdf ] Tutorial 4: identification of bound solvent ions in tetragonal lysozyme

	Portable Document Format (PDF) file (375.6 kbytes) [ doi:10.1107/S0021889808032494/kk5029sup5.pdf ] Tutorial 5: identification of a bound ligand in the active site of tetragonal lysozyme

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