Figure 2
Crystallization of lysozyme at 293 K in the lipidic cubic phase prepared with monoolein as the hosting lipid. (a) A view of a suitably positioned 200 nl mesophase (m) bolus surrounded by 1 µl of precipitant (p) solution in a 6 mm-diameter well (w). (b) A close-up view in normal light of the mesophase immediately upon setup. The edge (e) of the mesophase bolus is marked with an arrow. (c) An expanded view in normal light of the mesophase 30 min after setup. Small crystals are obvious as dark flecks around the perimeter of the bolus. (d) As in (c), viewed between crossed polarizers. (e) An expanded view of the mesophase in normal light 60 min after setup. Crystals are clearly visible as dark flecks around the perimeter of the bolus. (f) As in (e), viewed between crossed polarizers. Crystals are apparent as bright flecks on a dark background. (g) An expanded view in normal light of crystals growing in the lipidic mesophase 85 min after setup.  [article HTML]