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![[Figure 4]](to5062fig4mag.jpg)
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Figure 4
(Left) Simulated SANS curves obtained for BLG in D2O at 5 g l−1 with increasing urea concentration in solution (from bottom to top, open squares, circles, up-triangles, down-triangles and diamonds correspond to 0, 2, 4, 5 and 6 M urea, respectively) and their best fits obtained with GENFIT. All data were simulated at ambient pressure and temperature, at pD = 2.3, and at 20 mM ionic strength. The native BLG monomer and the unfolded chain are reported. The best fit parameters of the worm-like monomer were Kuhn length b = 4.6 ± 0.4 Å, inner cross section R = 4.1 ± 0.2 Å, number of statistical segments Nb = 90 ± 20 and relative mass density of the hydration shell dw = 0.951 ± 0.001. The best fit parameters of the unfolding free energy are ΔGunf,0 = 12 ± 1 kBT, ΔGunf,1 = −2.4 ± 0.2 kBT M−1 and ΔGunf,2 = 0.00 ± 0.03 kBT M−2. (Right) BLG folded monomer fraction in solution versus urea molar content as obtained from the calculated unfolding free energy. |
![[Figure 4]](to5062fig4.jpg)
 | JOURNAL OF APPLIED CRYSTALLOGRAPHY |
ISSN: 1600-5767
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