What macromolecular crystallogenesis tells us – what is needed in the future

Although the general rules governing protein crystal growth are known and a diversified panel of crystallization methods is available, producing focused protein crystals remains challenging. In the future, the supramolecular vision of crystallogenesis will break disciplinary boundaries and unify the field in a wider perspective beyond crystallization.

Crystallogenesis is a longstanding topic that has transformed into a discipline that is mainly focused on the preparation of crystals for practising crystallographers. Although the idiosyncratic features of proteins have to be taken into account, the crystallization of proteins is governed by the same physics as the crystallization of inorganic materials. At present, a diversified panel of crystallization methods adapted to proteins has been validated, and although only a few methods are in current practice, the success rate of crystallization has increased constantly, leading to the determination of $10 5 X-ray structures. These structures reveal a huge repertoire of protein folds, but they only cover a restricted part of macromolecular diversity across the tree of life. In the future, crystals representative of missing structures or that will better document the structural dynamics and functional steps underlying biological processes need to be grown. For the pertinent choice of biologically relevant targets, computerguided analysis of structural databases is needed. From another perspective, crystallization is a self-assembly process that can occur in the bulk of crowded fluids, with crystals being supramolecular assemblies. Life also uses selfassembly and supramolecular processes leading to transient, or less often stable, complexes. An integrated view of supramolecularity implies that proteins crystallizing either in vitro or in vivo or participating in cellular processes share common attributes, notably determinants and antideterminants that favour or disfavour their correct or incorrect associations. As a result, under in vivo conditions proteins show a balance between features that favour or disfavour association. If this balance is broken, disorders/diseases occur. Understanding crystallization under in vivo conditions is a challenge for the future. In this quest, the analysis of packing contacts and contacts within oligomers will be crucial in order to decipher the rules governing protein self-assembly and will guide the engineering of novel biomaterials. In a wider perspective, understanding such contacts will open the route towards supramolecular biology and generalized crystallogenesis.

Crystallogenesis in the time of physiology and chemistry
Protein 1 crystallization dates back to the 19th century, when physiology and chemistry were the leading sciences. The field started with the crystallization of various haemoglobins and plant globulins, and culminated in the period 1925-1936 with the crystallization of urease, pepsin and a few other enzymes (reviewed by Giegé, 2013). At this time crystallization was used as a purification tool and allowed James B. Sumner and John H. Northrop to show that the catalytic power of enzymes resides in the protein itself and not in organic catalysts adsorbed on the protein surface. Sumner and Northrop were rewarded with the 1946 Nobel Prize in Chemistry, which was shared with Wendell M. Stanley, who isolated Tobacco mosaic virus (TMV) in a crystalline form and subsequently showed that it retains activity after solubilization. These seminal discoveries opened the era of modern biochemistry and molecular biology. Furthermore, the observations on TMV meant the death of vitalism, when it became clear that viruses act as chemical molecules.
Important influences on crystal science were the physicochemical studies on the solubility of proteins in salts by Franz Hofmeister and the work by Wilhelm Ostwald on the transformation/ripening of solid materials in solution, for which he was awarded the 1909 Nobel Prize in Chemistry. While the relevance to biology of Ostwald ripening remained elusive for years, its detection during protein crystallization came late and its physicochemical understanding is recent (Streets & Quake, 2010). A typical example of ripening was found when exploring the phase diagram of Tomato bushy stunt virus (TBSV; Lorber & Witz, 2008;Fig. 1).
A paradigm change occurred in 1934 with the first X-ray photographs of crystalline pepsin (Bernal & Crowfoot, 1934), with the focus of macromolecular crystallization moving rapidly from physiology and chemistry to biology and physics.
2. Crystallogenesis in the time of biology and physics 2.1. A serious bottleneck when nascent molecular biology met X-ray crystallography In the early days of protein crystallography, when the methods for structure determination were first developed, the preparation of crystals appropriate for diffraction studies was not a major issue. The situation changed dramatically in the 1950s to 1960s, when the basic architectural elements of nucleic acids and proteins were discovered and when the nascent discipline of molecular biology aimed to understand the structure-function relationships in enzymology and the metabolic processes essential for life. This motivated structural biologists to attempt the targeted crystallization of enzymes, membrane proteins, tRNAs and supramolecular assemblies. However, given the multiparametric nature of the crystallization process and the difficulty in obtaining these compounds in the gram amounts required for the existing crystallization methods, it was rapidly realised that the growth of X-ray compatible crystals was a limiting factor. Thus, the current batch and dialysis methods were scaled down and vapour-diffusion and interface-diffusion methods were invented (Table 1). At the same time, new purification technologies were developed. Together, these allowed crystallization assays in the 10-50 ml range, so that projects could be started with amounts of protein in the 1-100 mg range. However, the success rate of crystallization (i.e. the number of successful trials compared with the total number of trials) was poor, and in practice increasing numbers of trials and much larger amounts of proteins were required. Furthermore, for most crystallized proteins the time between the first crystal and resolution of the three-dimensional structure was long, and could reach several years. As a result, only a few structures had been solved by 1980, corresponding to <0.1% of the presently deposited structures in the Protein Data Bank (PDB).

Better crystallization methods and still a bottleneck
For practical reasons and also as the outcome of interdisciplinarity, crystallization methods and strategies better adapted to proteins were invented and old forgotten methods were rejuvenated, for example crystallization in gelled media. This allowed the crystallization of ever-smaller amounts of protein via assays in ever-smaller volumes (from the microlitre to the nanolitre range). A diversified toolbox is now at the disposal of structural biologists (Table 1), enabling large-scale screening of crystallization parameters and growth optimization for enhancing the likelihood of obtaining well diffracting crystals (Giegé, 2013;Russo Krauss et al., 2013;Sauter et al., 2012). Note that the conventional methods (vapour diffusion, microbatch and incomplete factorial parameter screening) are still favoured by most experimentalists, and few structural biologists use more advanced methods. The latter, which are either physics-driven [for example counter-diffusion (Otá lora et al., 2009), gelled media (Lorber et al., 2009) Ostwald ripening of a cubic TBSV crystal in a precipitate in vitro (courtesy of B. Lorber). The crystal grew in a 20 ml drop; after 35 d its volume reached the mm 3 range. The clear halo around the crystal indicates that it grew at the expense of insoluble material. The images are at the same magnification. (Maeki et al., 2016) or stirring (Maki et al., 2008)] or biologydriven [for example the crystallization of fusion proteins (Ting et al., 2016) or novel co-crystallization strategies using chaperones selected from combinatorial libraries (Hipolito et al., 2014;Pardon et al., 2014)] are slowly infiltrating structural biology laboratories.
As a result, members of most protein classes and subclasses have been crystallized since the 1990s, and in the last two decades the number of successful crystallization attempts has increased tremendously, leading to the deposition of $1.1 Â 10 5 X-ray structures in the PDB. It could be falsely concluded that the crystallization bottleneck has been overcome. This is not the case, however, since these structures cover a restricted part of macromolecular diversity across the tree of life (Fig. 2a). Although the structures of many protein families from the three kingdoms of life are represented, the coverage is uneven. Clearly, the structures of membrane proteins, nucleic acids and protein-nucleic acid complexes are underrepresented (Fig. 2b) and those of other proteins are overrepresented (for example $550 structures of hen egg-white lysozyme and 250 of ferritins). Moreover, about half of the structures have been solved at medium or poor resolution, indicating that these structures were solved from crystals of rather weak diffraction quality (Fig. 2c).
Why is there this partial coverage of macromolecular diversity? It is likely to be owing to the idiosyncratic attributes of biomaterials, such as inherent plasticity, hydrophobicity of membrane proteins, or physicochemical and architectural characteristics of RNAs. Moreover, many proteins include unfolded domains or are intrinsically unstructured (Dyson & Wright, 2005). To add to the difficulties, the attributes of protein families are not clear-cut, such as the 'hydrophobicity' partly present in soluble proteins, implying that the crystallization methods developed for membrane proteins can be applied to soluble proteins (Caffrey, 2015).

The quest to understand and optimize
Since the early days of biological crystallography, pioneers have aimed to understand protein crystal growth. Today, it is accepted that the general principles governing the crystallization of inorganic materials also apply to the protein field (Vekilov & Chernov, 2002) and that diffusive mass transport during growth should be favoured, a condition that is unfortunately not fulfilled during crystallization by conventional methods. In addition, protein-specific parameters were found to be crucial, the most important being the protein itself (Giegé, 2013). Importantly, the two-step nucleation mechanism proposed for protein crystallization also applies outside the protein field, as critically discussed by experts in materials science (Erdemir et al., 2009). Like inorganic crystals, protein crystals grow by screw dislocation at low supersaturation and by the formation of two-dimensional islands at higher supersaturation. However, uniform growth is not guaranteed in the currently used crystallization setups (i.e. in vapour diffusion), since supersaturation decreases during growth, so that a single crystal can grow by the two mechanisms, as explicitly seen with tRNA Phe crystals (Ng et al., 1997). This produces internal stress in the crystals and affects mosaicity, as has been shown with lysozyme (Chernov, 1999), and therefore adversely affects the practical use of many macromolecular crystals. A remedy could be crystal growth at constant supersaturation, as can be performed in flow cells, although this is unfortunately not user-friendly.  Table 1 An overview of the methods and strategies for protein crystallization.
Adapted from Tables 4, 6 and 7 in Giegé (2013). Newer references are given here for co-crystallization with macrocyclic peptides (Hipolito et al., 2014), co-crystallization with nanobodies (Pardon et al., 2014) and the 'adsorption and desorption' method (Guo et al., 2014); the names of the authors and details are given within these four references.
(a) Crystallization methods. In the 1990s, when the demand for macromolecular crystals became crucial, pioneers tried to understand the factors that make many proteins resistant to crystallization. A few explored the inter-protein contacts in crystal lattices and compared them with the contacts occurring in oligomer interfaces. The examination of rather small sets of structures ($200 PDB entries) showed subtle differences in size and amino-acid composition within contact patches, with a tendency for smaller patches rich in polar residues and looser interactions in packing contacts (Janin & Rodier, 1995). This suggested a harmful role for surface lysine residues in proteins that are resistant to crystallization (Dasgupta et al., 1997).
Practical considerations motivated researchers to seek crystallization predictors and strategies to optimize the success rate of crystallization. Thus, physicochemical properties affecting crystal growth were sought from the crystallization data sets (!500 proteins) collected by structural genomics consortia, for example for Thermotoga maritima (Canaves et al., 2004) or from bacterial and human proteomes (Price et al., 2009). This drew attention to well ordered surface patches that mediate inter-protein interactions and to less-ordered surface patches, with high surface entropy, that lower the crystallization propensity (Derewenda & Vekilov, 2006;Price et al., 2009). In the same way, basic knowledge on crystal architectures was exploited to engineer proteins for enhanced crystallizability. Thus, knowledge of the packing contacts in ferritins guided the mutagenesis of human ferritin to enhance crystallization (Lawson et al., 1991). Likewise, surface-entropy reduction strategies, in which surface lysine, glutamine or glutamate residues with mobile side chains are replaced by smaller alanine residues, produced crystallizable mutants of proteins (Goldschmidt et al., 2014). In parallel, analyses of crystallization databases helped to optimize crystallization screens (Fazio et al., 2014), for example using PEG-based cocktails (Chaikuad et al., 2015). Another promising approach came from the improved success rate of liganded protein or nucleic acid crystallizations guided by biophysical diagnostics (Chung, 2007;Da Veiga et al., 2016). Another advance came from knowledge of the physical chemistry of concentrated protein solutions, which revealed the practical importance of the second virial coefficient (B 22 ). This coefficient informs about the attractive or repulsive interactions that favour or disfavour protein association under pre-crystallization conditions. Thus, slightly negative B 22 values point to globally attractive interactions favouring crystallization and positive values indicate repulsive interactions favouring aggregation. Thereby, B 22 became a useful predictor of the likelihood of proteins to crystallize (George et al., 1997).

The present status of crystallogenesis
Today, crystallogenesis is a discipline merging biology (mainly biochemistry and molecular biology), physics, chemistry and associated technologies, in which basic and applied aspects are equally important. Taking into account the chemical and structural peculiarities of biological macromolecules and given the universality of crystal-growth rules, methods have been devised to enhance the preparation of protein crystals suitable for structural biology. Yet, protein crystallization remains challenging, since most proteins are recalcitrant to crystallization.
Scattered reports over many years have suggested that the scope of macromolecular crystallogenesis extends beyond structural biology, but this perspective remains scarcely investigated. The existence of protein crystals that form in vivo and of proteic crystallites associated with human pathologies also pose questions regarding their formation in complex fluids that differ from the current crystallization media, in which protein purity is essential.

A pivotal period for breaking boundaries
In the present pivotal time for biological sciences, the challenges of crystallogenesis require more interdisciplinarity.  Among the known structures, 77% are solved at resolutions better than 2.5 Å , but only 9% are at resolutions better than 1.5 Å , notably $600 at subatomic resolution ( 1.0 Å ); only two proteins (the small crambin and an iron-sulfur protein) have been solved at ultrahigh resolution ($0.5 Å ).
Thus, the classical physicochemical picture of protein crystal growth should be expanded to be more focused on chemistry and biology. This should lead to novel ways to grow crystals to solve structures that are missing from the PDB. Advanced strategies could help to enhance the crystallization of partly unstructured proteins (for example upon the binding of selected ligands) and to grow crystals of proteins in apo and transiently liganded forms. Understanding why and how protein crystals grow in vivo is another challenge. This aspect is highlighted by the recent interest in such crystals in structural biology (Boudes et al., 2016). Finally, crystallization still needs to be refined for serial femtosecond and X-ray freeelectron laser crystallography (SFX and XFEL; Levantino et al., 2015) and for the renewed use of neutron crystallography (Blakeley et al., 2015).
The time is also ripe to unify the outcomes of macromolecular crystallogenesis, which so far mainly concern structural biology, to integrate topics that are seemingly beyond the periphery of structural biology. Shifting the foundations of the discipline towards chemistry could do this. This implies a supramolecular vision of protein crystallization. If protein crystallization is seen as a self-assembly process, it can be approached from the conceptual background of supramolecular chemistry. As emphasized by Jean-Marie Lehn: supramolecular chemistry aims at implementing highly complex chemical systems from molecular components held together by non-covalent intermolecular forces and effecting molecular recognition, catalysis and transport processes (Lehn, 2012). This applies perfectly to macromolecular crystals, including crystals of viruses and huge molecular machines (note that catalysis can occur in cristallo). Molecular recognition, catalysis and transport processes are also keywords in molecular and cellular biology. Logically, this means that protein crystallization and molecular biology are based on similar supramolecular rules. Knowledge of such rules would immediately find application in understanding protein crystal growth in living organisms (Doye & Poon, 2006). Furthermore, it is tempting to suggest that protein crystals represent simplified model systems, from which can be deciphered general rules underlying the more complex molecular assemblies and functional processes occurring in living organisms. Going one step ahead, one can anticipate that macromolecular crystallogenesis will provide insights into an emerging supramolecular biology.
4. What is needed in the future?

Prologue
Two lines of research trace the future of macromolecular crystallogenesis. Firstly, efforts to improve the growth of crystals for structural biology in order to better understand the universe of proteins and RNAs, to refine the molecular-based taxonomy of species by filling gaps in the three-dimensional space of the tree of life and to understand four-dimensional structures (with time as the fourth parameter). Secondly, progress towards the world of supramolecularity with crystals seen as self-assembled entities with peculiar stability and plasticity.
Future developments will depend on technological and computational advances and will also benefit from structures solved by cryo-EM. For a rational choice of crystallization conditions, novel tools will facilitate comparison of the outcomes of crystallization experiments (Bruno et al., 2014). Other tools are aimed towards a microscopic picture of the dynamics of protein crystals and a realistic modelling of crystallization conditions (Kuzmanic & Zagrovic, 2014) and, more radically, towards computational tools to guide crystallization (Altan et al., 2016). Furthermore, new methods will help in the preparation of nanocrystals suitable for data collection on SFX and XFEL instruments (Boudes et al., 2016). Also, the toolbox for mining structure and sequence databases is being enlarged, for example for automated evolution-based assessment of protein-protein interfaces (Baskaran et al., 2014). Finally, the tools developed in softmatter physics for studying colloidal assemblies will offer new insights into problems of protein crystallization (Fusco & Charbonneau, 2016).

Short-term perspectives within and outside structural biology
4.2.1. Towards a refined understanding of the structural landscape of proteins. Different types of crystals are needed: firstly, crystals representative of protein families that are under-represented in or missing from the PDB and, secondly, crystals of proteins from taxonomic branches that are poorly represented or absent in the PDB, as well as crystals of the novel proteins that are continually being discovered from microbiotes and metagenomes. Also, crystals of proteins encoded in the genomes of giant viruses are expected. These will offer a timely opportunity to enter into the new biology of these viruses (Claverie & Abergel, 2016). The resulting structures will provide new insights into evolution and will allow a better correlation of genome-based or organism-based phylogenies with structure-based phylogenies.
Analysis of genomic databases, which are much larger than the PDB, should provide guidelines for pertinent choices of the proteins to be crystallized (for example from the proteomes of eukaryotic human pathogens). Also, the crystallization of prokaryal-like mitochondrial proteins, which are likely to be different from their human cytosolic homologues, is of interest for rational drug design and applications in medicine. On the other hand, since many protein structures are partly disordered, stable domains should be dissected from genes and overexpressed for crystallization. Flexible or unstructured proteins could also be stabilized in natural or artificial complexes that are more prone to crystallization. Using macrocyclic peptides (Hipolito et al., 2014) or camelid antibody fragments (i.e. nanobodies; Pardon et al., 2014) as specific co-crystallization chaperones selected from combinatorial libraries may be strategies of universal application.

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Preparation of protein crystals of special biological interest in apo and liganded forms can be challenging. Such crystals with functional states that can be captured in cristallo are needed to uncover the dynamics of biological processes at the molecular level (for example during allosteric motions, conformational adaptations in complexes and enzymatic reactions). To develop plausible kinematic pathways based on sets of transient structures, crystal polymorphs are needed for each step in the functioning of the selected proteins. Resolution of their structures will allow functional effects (conserved in polymorphs) to be distinguished from packing 'artefacts' (not conserved). Finding the same effects in homologous proteins will provide additional support. Alternatively, timeresolved SFX and XFEL crystallography could bring quick answers, but is not of general application because only small motions can be detected in the crystalline environment and because few systems allow in situ initiation of their reactions concomitant with the X-ray pulses (Levantino et al., 2015).
Another aspect concerns the optimization of crystal perfection. For this purpose, several approaches are possible, most based on crystal growth under diffusive regimes (for example counter-diffusion and gelled media). To guarantee growth under a uniform regime, control of the thermodynamics and kinetics at all stages of crystallization is needed. Diagnostic tools [for example dynamic light scattering (DLS), small-angle X-ray scattering (SAXS), calorimetry, interferometries and microscopies] and computational database mining are essential to uncover the relative importance of the crystallization parameters. Finally, efficient methods to generate tiny crystals for SFX and XFEL crystallography and large crystals for neutron crystallography are needed. Protein nanocrystals can be found and characterized in reversible precipitates or produced on purpose, for example in special batch systems controlled by DLS (Schubert et al., 2015). Large crystals can be grown by methods based on dialysis or counterdiffusion (Fig. 3). In one strategy, crystallization occurs in a temperature-controlled flow-cell dialysis system (Junius et al., 2016). In another, large crystals are grown by counterdiffusion and Ostwald-like ripening in capillaries of large diameter. Because of their large diameter, diffusive mass transport is not optimal on Earth, but can be enhanced under so-called microgravity environments, thereby leading to enhanced crystal size and perfection (Ng et al., 2015).

Implications for materials science, biotechnology
and molecular medicine. A few applications of these ideas outside structural biology have already emerged and others are awaited. For instance, antibodies could be used as sensors of two-dimensional and three-dimensional organized crystalline surfaces. Recognition of crystals composed of relatively small organic molecules, such as cholesterol monohydrate, by selected monoclonal antibodies provided a proof of concept (Addadi et al., 2008). The concept may apply to protein crystals, but this awaits confirmation.
Self-assembly rules will guide the engineering of novel nanoscaled materials. For nucleic acids, the idea was proposed by Seeman in the 1980s and has been exploited for the preparation of self-assembled DNA crystals (Zheng et al., 2009) and of photonic crystals developed through DNAprogrammable assembly (Park et al., 2015). Such crystals can have multiple applications in biology and materials science (Jones et al., 2015). In the protein field, self-assembly processes are ubiquitous, but have attracted attention only recently. Seen from a soft-matter perspective, their study is essential to understand protein-condensation diseases (e.g. Alzheimer's disease) and biotechnological purification processes based on liquid-liquid phase separation, as well as being important for protein crystallization (McManus et al., 2016).
Engineering protein crystals is another approach. One method aims to deliver crystals of fusion proteins constituted of a cargo (the Cry3Aa protein from Bacillus thuringiensis) fused to reporter proteins. Such fusions grown in the bacterium can be taken up in vitro by different cell lines or delivered to mice in vivo via many modes of administration (Nair et al., 2015). In another strategy, protein crystals are  functionalized in vitro with metallic or organic compounds, as shown with ferritin and lysozyme crystals that were converted into catalytic, magnetic, luminescent or fluorescent nanoparticles (Abe et al., 2016).

Trends towards supramolecular crystallogenesis
4.3.1. Supramolecularity guided by chemistry and physical chemistry. Self-assembly and supramolecular processes are seminal attributes of life. Therefore, an integrated understanding of supramolecularity implies that macromolecular entities that crystallize or participate in biochemical processes require discrete physical and chemical determinants and antideterminants that favour or disfavour correct or incorrect molecular recognition. The search for determinants appears to be most immediate, and is well documented, in biochemistry (e.g. Giegé & Eriani, 2014), but is much less so in crystallogenesis. Antideterminants, on the other hand, are poorly known, although the first findings came from studies performed in the 1990s on the crystallization of ferritins (Lawson et al., 1991). Thus, analysis of the packing contacts in horse ferritin crystals and the sequence comparison of three ferritins indicated that Asp84 and Gln86 are crystallization determinants and that Lys86 is an antideterminant. Given the conceptual similarities to biochemistry, this suggests that the recognition patterns in proteins or nucleic acids interacting in crystal lattices and in solution are in part similar.
Approaching the question of molecular recognition from the perspective of physics should provide global answers. However, until now the noncovalent binding thermodynamics of inter-protein interactions have essentially been addressed separately for proteins in the crystalline state and in solution.
Consequently, an integrative understanding that merges both crystal and solution aspects has not yet emerged. Nevertheless, it has been proposed that proteins in the crystalline state are more stable than in solution (Drenth & Haas, 1992) and that low side-chain entropy of surface residues is a significant determinant of crystallization propensity, although this propensity is not strongly influenced by the overall thermo-dynamic stability of proteins (Price et al., 2009). Recently, based on softmatter physics, it has been shown that the geometrical asymmetry of patches on protein surfaces weakly affects crystallization, in contrast to the bondenergy asymmetry that markedly interferes with crystallization thermodynamics and kinetics (Fusco & Charbonneau, 2013).
A promising connection between solution and crystal physics came from a mechanism for controlled protein interactions mediated by multivalent metal cations that activate attractive patches on protein surfaces, thereby facilitating the formation of interprotein ion bridges in solution and in crystals (Roosen-Runge et al., 2014). The implication is that inter-protein ion bridges favour crystallization. A crystallization method termed 'metal-mediated synthetic symmetrization' supports this possibility, since the introduction of histidine or cysteine residues on protein surfaces for coordination with metal ions triggers crystallization (Laganowsky et al., 2011). This is reminiscent of the old observation on the role of Ca 2+ ions that mediate the crystallization of ferritins (Lawson et al., 1991).
Other openings have come from data mining of the PDB. Comparison of packing interfaces and biological interfaces in monomeric and homodimeric proteins showed that large crystal-packing contacts have interface areas and contact sizes similar to those of permanent homodimers ( Table 2). The properties of these packing contacts show similarities to the weak transient complexes occurring in nonpermanent homodimers, as reflected by the number of hydrogen bonds and noncovalent contacts (ionic, hydrophobic, and van der Waals). Moreover, packing contacts appear to be more loosely organized, with less hydrophobic interactions than in the permanent subunit contacts in homodimers (Luo et al., 2015). Generalizing these conclusions is presently premature since the analyses were performed on a limited number of structures. However, the trend is promising and calls for further computational studies to better comprehend the structural principles underlying packing or recognition rules. For this purpose, the amino-acid or nucleotide residues involved in crystal formation owing to direct or indirect (metal-ion or water-mediated) weak noncovalent interactions should be more systematically characterized on larger and well defined macromolecule families.
On the experimental side, the engineering of protein surfaces or the mutagenesis of packing contacts in crystals is needed to decode the recognition rules underlying protein crystallizability. Several proof-of-concept studies provide guidelines for the future. Thus, a preliminary study in the 1990s on the crystallizability of thymidylate synthase showed that mutating single surface amino acids yields crystal polymorphs with dramatically altered solubilities (McElroy et al.,  Table 2 Towards understanding the geometric and physicochemical properties of the protein surfaces seen in crystals: a precursory proof-of-concept study with a view to finding crystallization indicators. Adapted from Luo et al. (2015). Asterisks indicate statistical significance. 1992). Another study showed that the bovine pancreatic trypsin inhibitor crystallizes in polymorphs assembled from monomers or decamers as the result of oligomeric changes that occur under pre-crystallization conditions (Hamiaux et al., 2000). Also noteworthy was the engineering of the ParBlike nuclease by reductive methylation of surface lysine residues, which created new intermolecular and intramolecular contacts and thus resulted in well diffracting crystals with enhanced packing and protein stability (Shaw et al., 2007).
Neglected but essential aspects concern the solvent content of macromolecular crystals and solvent effects that influence the activity, stability and intermolecular interactions of macromolecules. Solvent constitutes about half of the crystal volume on average, and up to 80% in extreme cases. Disordered bulk solvent allows in cristallo molecular flexibility and in some cases even functional activity. However, part of the solvent is ordered and forms a hydration shell around proteins (Kim et al., 2016;Weichenberger & Rupp, 2014). This shell has a patchy organization, which is likely to be complementary to the patches on protein surfaces. According to SAXS and SANS data, solvent density modifications occur at protein surfaces, as seen for the green fluorescent protein.
Thus, the hydration shell is locally denser in the vicinity of acidic surface residues, while hydrophilic, hydrophobic and basic residues modify the density only mildly. These modifications result from the combined effects of residue-specific ion recruitments from the bulk solution and water structural rearrangements (Kim et al., 2016). Similarly, in crystals containing nucleic acids, both anions  and cations  stabilize the structure of DNA or RNA.

Supramolecularity guided by evolution and integra-
tive biology. Several facts directly related to evolution have to be taken into account to understand macromolecular crystallization. Firstly, evolution has only explored a small part of the potential diversity of the sequence space of proteins and nucleic acids. Ancient sequences were likely to be short and compatible with folding into more or less stable conformers. In the course of evolution these sequences became larger, mainly by the fusion of structural domains. This is well exemplified when comparing proteins with the same function from bacteria and higher eukarya. Secondly, protein crystals have been observed in many organisms from all kingdoms of life, indicating that crowded biological fluids are not inevitably harmful for protein crystallization (Doye & Poon, 2006). This fact, which seemingly contradicts the current belief that purity favours crystallization, was long neglected. Nevertheless, most proteins remain soluble in vivo. This contrasting truth has been conceptualized in the 'evolutionary negative design' principle (Doye et al., 2004). Thus, protein sequences and physicochemical parameters are proposed to have evolved to avoid crystallization in biological media. If the molecular composition and physicochemical parameters of these media are modified, crystallization or aggregation of proteins may occur. This implies a balance between features that favour or disfavour self-association for each protein present in a given biological fluid. If this balance is broken by modification of protein concentration, mutation or a change in the physicochemical properties of the fluids, functional disorders may occur (for example in human diseases associated with crystalline or aggregated phases). Such functional disorders often occur in expression systems in which inclusion bodies can contain aggregated or microcrystalline proteins, especially when the expression levels of the target proteins are too high.
Furthermore, and as a consequence of sequence properties, evolution has determined the architecture, structural stability and dynamics of proteins, as well as the structural organization of multi-macromolecular systems. Symmetry and especially asymmetry are often invoked when describing such systems. Thus, structural symmetry provides stability, as in spherical viruses or in ferritins, but is rare, while asymmetry is frequent and occurs in dynamic systems (Blundell et al., 2002). In other words, as quoted by art historians, 'symmetry signifies rest and binding and asymmetry motion and loosening' (McManus, 2005). These attributes apply to crystallogenesis, since the crystallization of symmetric structures is easier than that of asymmetric structures, as reflected by the over-representation of symmetric structures in the PDB. Consequently, symmetry should stabilize crystals and help crystallization. This conjecture was verified by crystallizing proteins after symmetrization (Laganowsky et al., 2011). Likewise, deliberate reduction of asymmetry should help crystallization, for example by the removal of post-translational modifications. This was first verified in the 1990s by the crystallization of glycoproteins after enzymatic deglycosylation (Baker et al., 1994). However, this is at the expense of lost biological information, and hence novel methods to overcome this bottleneck are awaited. This is especially true for post-transcriptionally modified RNAs.
When supramolecular crystallogenesis is seen from the viewpoint of integrative biology, one has to consider the consequences of cellular crowding on protein self-assembly. This is challenging, since the total concentration of protein and RNA inside, for example, an Escherichia coli cell is $300-400 mg ml À1 (Ellis, 2001). Despite such conditions, individual proteins and even large macromolecular assemblies can crystallize in crowded media (Doye & Poon, 2006). On the other hand, proteins participate in many inter-protein associations in crowded media, as is found in interactomes. For instance, many binary protein-protein interactions have been identified for the 726 proteins encoded in the small genome of the syphilis spirochete Trypanema pallidom (Titz et al., 2008). Yet, precise characterization of the contact patches that mediate these interactions is difficult, since only 36 crystal structures from the spirochete are present in the PDB. Other interactomes list partners of important target proteins, such as the phosphatase from Plasmodium falciparum, an enzyme that is essential for the viability of the parasite. This enzyme interacts with 134 partners (Hollin et al., 2016), but among the 530 known three-dimensional structures of plasmodial proteins that of the phosphatase is missing as well as those of most of its interaction partners. These examples show that many more three-dimensional structures are needed to assess the structural basis of the protein-protein interactions in interactomes.

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Altogether, this points to the significance of the patchy organization of protein surfaces. It is likely that surface patches represent evolutionary adaptations to sustain the multiple interactions that proteins make during cellular life, either for structural or functional reasons. This leads to a universe of interactions, which are essentially uncharacterized, and gives sense to the variable strength of inter-protein or inter-patch contacts. Obviously, the interplay of these interactions is crucial for crystallization (Fusco & Charbonneau, 2013).
These considerations emphasize the need to understand the organization of matter in living systems. Clearly, the location of proteins is not completely disordered in biological fluids and their ordering is necessarily enhanced in crystals. In between, proteins can occur in a variety of mesophases (lyotropic membranes, two-dimensional crystals, liquid crystals, spherulites and other paracrystallites). In such intermediate states between solid and liquid, the principal molecular properties of ordered structures are maintained, but structural rigidity decreases while kinetic disorder and entropy increase. Such possibilities were suspected long ago (Bernal & Crowfoot, 1933), but only recently has their biological importance been appreciated (Hyde, 2015) with attempts to gain knowledge from a soft condensed matter perspective (McManus et al., 2016). At present, most questions about the fate and organization of matter under biologically relevant conditions await answers. For example: what are the proteins that are recalcitrant to crystallization in cellulo? Or can all proteins crystallize in vivo? To what extent are contact patches involved in crystal packing also involved in interactome interactions? What are the proteins most commonly found in macromolecular assemblies? What are the transient assemblies mostly found in vivo?
Given the diversity of possible interactions, it is worthwhile identifying the supramolecular parameters that modulate the strength of the inter-patch contacts and deciphering their exact chemical nature. This requires precise computational analyses of protein surfaces and more crystals (with interactome-guided selection of the proteins) for structural analyses.

Epilogue: towards supramolecular biology and generalized crystallogenesis
To conclude, a visionary sentence, written 25 years ago in a paper on the stability of protein crystals, must be highlighted: Protein crystals are not only important for the crystallographer, but they have more virtue. One day they may even play a role in the material sciences or in electric circuits . . . (Drenth & Haas, 1992). Today, this prophecy has been fulfilled and new disciplines percolate macromolecular crystallogenesis, such as supramolecular chemistry (Uhlenheuer et al., 2010) and soft-matter sciences (Fusco & Charbonneau, 2016). In a wider perspective, self-assembly recognition rules open the route to supramolecular biology. Here, one is faced with a continuum of interactions, from strong to weak (essentially transient), occurring in cellular mesophases. Phase transitions play crucial roles in such self-assembly processes (Lee et al., 2013). Understanding packing and oligomer contacts should provide operational models for a wider understanding of functional in cellulo interactions. To this end, physical and chemical determinants and antideterminants that control selfassembly will be crucial.
Finally, the intricate organization of biomolecular entities in dynamic mesophases points to the notion of structural order in biological systems. Such order correlates with the existence of different types of symmetric and pseudo-symmetric patterns that are regularly found in biosystems ranging from microscale and nanoscale patterns (within proteins and nucleic acids, oligomers, viruses and multi-macromolecular assemblies) up to macroscale patterns (within cellular and organismic phenotypes). The need to understand their nature and genesis explains the emergence of a generalized crystallography (Hyde, 2015) and, better, of a generalized crystallogenesis where biology, chemistry, physics and even aesthetics are intimately interwoven.