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Figure 2
Relationship between the number of incident X-ray photons and the resolution achieved. These datasets were collected on beamline BL32XU at SPring-­8 from nine thaumatin crystals with sizes of ∼100 µm. From each crystal, five datasets, each consisting of 100° of rotation, were collected from the same crystal volume with different numbers of incident photons, 9.8 × 109 (0.1 MGy), 6.4 × 1010 (0.2 MGy), 1.9 × 1011 (0.5 MGy), 2.8 × 1011 (1.0 MGy) and 1.8 × 1012 (5.0 MGy), at an energy of 12.3984 keV using a 10 × 15 µm beam. All datasets were processed with XDS (Kabsch, 2010aBB47,bBB48) and the resolution limit of each dataset was determined so that 〈I/σ(I)〉 in the highest shell was ∼2. Averaged resolution limits using nine crystals with error bars showing standard deviations are plotted as d*2 against the number of incident photons. Thaumatin was crystallized by the microseeding method based on the standard crystallization condition (Mueller-Dieckmann et al., 2005BB60).

Volume 4| Part 5| September 2017| Pages 529-539
ISSN: 2052-2525