Journal of Synchrotron Radiation

Volume 6, Part 4 (July 1999)


Synchrotron Radiation and Structural Biology

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[Issue Author Index][Volume Author Index]
[Cover illustration] Cover illustration: A schematic representation of the structure of F1-ATPase and Sir John Walker's prize-winning team, see Hasnain, Helliwell and Kamitsubo, pages 809-812.

editorial


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J. Synchrotron Rad. (1999). 6, 809-811  [ doi:10.1107/S0909049599007232 ]

Synchrotron radiation and structural biology

S. S. Hasnain, J. R. Helliwell and H. Kamitsubo

Online July 1999


research papers


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J. Synchrotron Rad. (1999). 6, 813-815  [ doi:10.1107/S0909049599006706 ]

Introductory overview

L. N. Johnson and T. L. Blundell

Online July 1999


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J. Synchrotron Rad. (1999). 6, 816-821  [ doi:10.1107/S0909049599005075 ]

Synchrotron radiation as a tool for investigating virus structures

M. G. Rossmann

Synopsis: Synchrotron radiation is critically important to the determination of virus structures. Methods of data reduction and phase determination are explained. Some examples are mentioned.

Online July 1999


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J. Synchrotron Rad. (1999). 6, 822-833  [ doi:10.1107/S0909049599006172 ]

Development of instrumentation and methods for MAD and structural genomics at the SRS, ESRF, CHESS and Elettra facilities

A. Cassetta, A. M. Deacon, S. E. Ealick, J. R. Helliwell and A. W. Thompson

Synopsis: Two decades of synchrotron radiation source, instrumentation and methods evolution have greatly accelerated the pace of protein crystallography, which can, via the linking up of synchrotron radiation `PX stations' worldwide, now yield a `genome level' of numbers of experimentally determined protein structures.

Online July 1999


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J. Synchrotron Rad. (1999). 6, 834-844  [ doi:10.1107/S0909049599006688 ]

Better structures from better data through better methods: a review of developments in de novo macromolecular phasing techniques and associated instrumentation at LURE

R. Fourme, W. Shepard, M. Schiltz, T. Prangé, M. Ramin, R. Kahn, E. de la Fortelle and G. Bricogne

Synopsis: The article is a review of developments in de novo phasing methods and instrumentation in macromolecular crystallography carried out at LURE, the French synchrotron radiation facility.

Online July 1999


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J. Synchrotron Rad. (1999). 6, 845-851  [ doi:10.1107/S0909049599007591 ]

Maturation of MAD phasing for the determination of macromolecular structures

W. A. Hendrickson

Synopsis: The course of developments in methodology for multiwavelength anomalous diffraction (MAD) is discussed, and characteristic traits of current applications are analyzed.

Online July 1999


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J. Synchrotron Rad. (1999). 6, 852-864  [ doi:10.1107/S0909049599006792 ]

Structure of metal centres in proteins at subatomic resolution

S. S. Hasnain and K. O. Hodgson

Synopsis: XAFS provides local structure of the metal site at subatomic resolution and is equally applicable to both aqueous and crystalline samples. This review provides some recent case studies to illustrate its synergy with protein X-ray crystallographic determination. This synergy exists not only for the scientific objective of structure-based understanding of proteins' function but extends to the source and general instrument requirements, particularly with MAD.

Online July 1999


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J. Synchrotron Rad. (1999). 6, 865-874  [ doi:10.1107/S090904959900669X ]

Bluetongue virus: the role of synchrotron radiation

J. M. Diprose, J. M. Grimes, P. Gouet, R. Malby, J. N. Burroughs, J. Lescar, B. Rassmussen, P. P. C. Mertens and D. I. Stuart

Synopsis: Observations from the structure determination of the bluetongue virus core are presented. Aspects of synchrotron radiation which enable the successful analysis of such complex systems are discussed.

Online July 1999


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J. Synchrotron Rad. (1999). 6, 875-890  [ doi:10.1107/S0909049599007025 ]

Muscle contraction: challenges for synchrotron radiation

K. Wakabayashi and N. Yagi

Synopsis: Recent X-ray diffraction/scattering studies on the actomyosin motor system are described and future challenges for synchrotron radiation are discussed.

Online July 1999


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J. Synchrotron Rad. (1999). 6, 891-917  [ doi:10.1107/S0909049599006366 ]

Laue crystallography: coming of age

Z. Ren, D. Bourgeois, J. R. Helliwell, K. Moffat, V. Srajer and B. L. Stoddard

Synopsis: Major developments in the theory of the Laue diffraction technique and of the necessary software, hardware and experimental strategies have led to successful time-resolved studies on the dynamic properties of a range of biological systems, which shed light on future applications of this technique.

Online July 1999


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J. Synchrotron Rad. (1999). 6, 918-927  [ doi:10.1107/S0909049599006184 ]

Membrane proteins: structure and function

T. Tsukihara and S. J. Lee

Synopsis: The structure-function relationships of integral membrane proteins so far determined by X-ray and electron crystallographic methods are described.

Online July 1999


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J. Synchrotron Rad. (1999). 6, 928-941  [ doi:10.1107/S0909049599006664 ]

The identification of selected components in electron density maps of prokaryotic ribosomes at 7 Å resolution

F. Schlünzen, I. Kölln, D. Janell, M. Glühmann, I. Levin, A. Bashan, J. Harms, H. Bartels, T. Auerbach, M. Pioletti, H. Avila, K. Anagnostopoulos, H. A. S. Hansen, W. S. Bennett, I. Agmon, M. Kessler, A. Tocilj, S. Krumbholz, M. Peretz, S. Weinstein, F. Franceschi and A. Yonath

Synopsis: The MIR map pf the small ribosomal subunit, constructed at 7.2 Å and extended to 5.5 Å using crystals diffracting to 3 Å, shows morphologies close to the active conformation of these particles and contains elongated dense features traceable as RNA chains as well as globular regions in which folds observed in isolated ribosomal proteins were detected. Surface proteins and selected RNA strands are being located by difference Fourier methods, exploiting the metal cluster attached to them.

Online July 1999


notes and news


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J. Synchrotron Rad. (1999). 6, 943-944  [ doi:10.1107/S0909049599007220 ]

News and reports

Online July 1999


J. Synchrotron Rad.. (1999). 6, 945

forthcoming meetings and short courses.


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