Journal of Synchrotron Radiation

Volume 9, Part 6 (November 2002)


Includes papers presented at the Second International Workshop on X-ray Damage to Crystalline Biological Samples

Chicago, USA, December 2001

[Buy print version] A printed copy of this issue is available for purchase


[Issue Author Index][Volume Author Index]
[Cover illustration] Cover illustration: Cryocooling a lysozyme crystal in a 100 K gas stream, see Snell, Judge, Larson and van der Woerd, pages 361-367.

radiation damage workshop


 

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J. Synchrotron Rad. (2002). 9, 327-328  [ doi:10.1107/S0909049502014565 ]

Radiation damage to crystalline biological molecules: current view

E. Garman and C. Nave

Synopsis: A short summary of the current state of knowledge regarding radiation damage to crystalline biological specimens is provided, taking into account information provided in recently published papers.

Online 1 November 2002


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J. Synchrotron Rad. (2002). 9, 329-332  [ doi:10.1107/S0909049502014553 ]

Physical and chemical considerations of damage induced in protein crystals by synchrotron radiation: a radiation chemical perspective

P. O'Neill, D. L. Stevens and E. Garman

Synopsis: The chemical processes possible when protein crystals suffer radiation damage in an X-ray beam are outlined. Approaches to reducing the damage are discussed from a radiation chemistry viewpoint.

Online 1 November 2002


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J. Synchrotron Rad. (2002). 9, 333-341  [ doi:10.1107/S0909049502014528 ]

Too hot to handle? Synchrotron X-ray damage of lipid membranes and mesophases

V. Cherezov, K. M. Riedl and M. Caffrey

Synopsis: X-ray damage to lipidic mesophases is shown to be free-radical mediated, to involve chemical degradation, to trigger phase transitions and to continue post-irradiation. Dose rate, X-ray energy, beam heating and dispersing-medium effects were investigated. X-ray diffraction is not a reliable indication of radiation damage. This work has relevance to physiological processes, such as aging, that involve oxidative damage.

Online 1 November 2002


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J. Synchrotron Rad. (2002). 9, 342-346  [ doi:10.1107/S0909049502014589 ]

Evidence for the formation of disulfide radicals in protein crystals upon X-ray irradiation

M. Weik, J. Bergès, M. L. Raves, P. Gros, S. McSweeney, I. Silman, J. L. Sussman, C. Houée-Levin and R. B. G. Ravelli

Synopsis: Changes in disulfide bond lengths upon X-ray irradiation are examined. Together with microspetrophotometric data and quantum chemical calculations, they are found to be indicative of the formation of disulfide radicals.

Online 1 November 2002


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J. Synchrotron Rad. (2002). 9, 347-354  [ doi:10.1107/S0909049502014632 ]

Investigation of possible free-radical scavengers and metrics for radiation damage in protein cryocrystallography

J. Murray and E. Garman

Synopsis: Unit-cell volume expansion is investigated as a radiation-damage metric in cryocooled protein crystals and found not to be a reliable indicator. The radical scavengers styrene and ascorbate are tested as means of reducing X-ray damage at 100K: styrene is found to be ineffective, but ascorbate is a promising candidate.

Online 1 November 2002


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J. Synchrotron Rad. (2002). 9, 355-360  [ doi:10.1107/S0909049502014541 ]

Unit-cell volume change as a metric of radiation damage in crystals of macromolecules

R. B. G. Ravelli, P. Theveneau, S. McSweeney and M. Caffrey

Synopsis: The unit-cell volume increase upon radiation damage has been used to study the effect of temperature and X-ray dose rate on crystals of holoferritin. A dose-rate effect has been found, and it is postulated that trapped radicals become mobile at ca 180K.

Online 1 November 2002


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J. Synchrotron Rad. (2002). 9, 361-367  [ doi:10.1107/S090904950201453X ]

Seeing the heat - preliminary studies of cryocrystallography using infrared imaging

E. H. Snell, R. A. Judge, M. Larson and M. J. van der Woerd

Synopsis: In this work it is shown for the first time that it is possible to thermally image macromolecular crystals cooled in a 100K nitrogen gas stream.

Online 1 November 2002


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J. Synchrotron Rad. (2002). 9, 368-374  [ doi:10.1107/S0909049502014620 ]

Investigation of radiation-dose-induced changes in organic light-atom crystals by accurate d-spacing measurements

R. Müller, E. Weckert, J. Zellner and M. Drakopoulos

Synopsis: The temperature, radiation energy and flux dependence on the rate of the change in d-spacing were investigated in order to monitor radiation damage in organic small molecules and protein crystals.

Online 1 November 2002


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J. Synchrotron Rad. (2002). 9, 375-381  [ doi:10.1107/S0909049502014577 ]

Experiments testing the abatement of radiation damage in D-xylose isomerase crystals with cryogenic helium

B. L. Hanson, J. M. Harp, K. Kirschbaum, C. A. Schall, K. DeWitt, A. Howard, A. A. Pinkerton and G. J. Bunick

Synopsis: Ultrahigh-resolution data collected from D-xylose isomerase crystals suggest that, when cooled with He rather than N2, these crystals maintain order and that high-resolution data are less affected by increased radiation load. An apparent phase transition in macromolecular crystals between 16 and 100K requires investigation to broaden cryogenic He use.

Online 1 November 2002


research papers


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J. Synchrotron Rad. (2002). 9, 383-393  [ doi:10.1107/S0909049502016308 ]

Propagation of a partially coherent focused X-ray beam within a planar X-ray waveguide

J. H. H. Bongaerts, C. David, M. Drakopoulos, M. J. Zwanenburg, G. H. Wegdam, T. Lackner, H. Keymeulen and J. F. van der Veen

Synopsis: A linear-transmission Fresnel-zone-plate lens is used for coupling a monochromatic X-ray beam of 13.2keV into a planar X-ray waveguide. The coherent properties of the beam are investigated by comparing the experimental data with numerical calculations in which partial coherence is taken into account by propagation of the mutual intensity function.

Online 1 November 2002


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J. Synchrotron Rad. (2002). 9, 394-400  [ doi:10.1107/S0909049502014000 ]

Oxygen-metal bonding in Ti-bearing compounds from O 1s spectra and ab initio full multiple-scattering calculations

Z. Wu, E. Paris, F. Langenhorst and F. Seifert

Synopsis: The O K-edge spectra of a series of Ti-bearing compounds with Ti in different structural and chemical environments are measured using electron energy-loss specroscopy and are analyzed using ab initio full multiple-scattering calculations.

Online 1 November 2002


computer programs


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J. Synchrotron Rad. (2002). 9, 401-406  [ doi:10.1107/S0909049502015170 ]

Blu-Ice and the Distributed Control System: software for data acquisition and instrument control at macromolecular crystallography beamlines

T. M. McPhillips, S. E. McPhillips, H.-J. Chiu, A. E. Cohen, A. M. Deacon, P. J. Ellis, E. Garman, A. Gonzalez, N. K. Sauter, R. P. Phizackerley, S. M. Soltis and P. Kuhn

Synopsis: The Blu-Ice and Distributed Control System software packages unify control over hardware at macromolecular crystallography beamlines and provide a platform for increasing beamline ease of use, automation and remote accessibility.

Online 1 November 2002


 

J. Synchrotron Rad. (2002). 9, 407

forthcoming meetings and short courses


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