Journal of Synchrotron Radiation

Volume 14, Part 1 (January 2007)


Includes papers presented at the Fourth International Workshop on X-ray Radiation Damage to Biological Crystalline Samples

Harima, Japan, 7-8 March 2006

[Buy print version] A printed copy of this issue is available for purchase


[Issue Author Index][Volume Author Index]
[Cover illustration] Cover illustration: The absorbance spectra of a disulfide bond in the absence and presence of a radioprotectant molecule during X-ray exposure (see Southworth-Davies and Garman, pages 73-83). Centre: protein crystal after X-ray irradiation at 100 K and brief annealing at room temperature. Bubbles of gas may contain CO2 originating from the decarboxylation of glutamic and aspartic acid residues (see Fioravanti, Vellieux, Amara, Madern and Weik, pages 84-91).

radiation damage


 

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J. Synchrotron Rad. (2007). 14, 1-3  [ doi:10.1107/S0909049506053015 ]

Progress in research into radiation damage in cryo-cooled macromolecular crystals

E. F. Garman and S. M. McSweeney

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 4-10  [ doi:10.1107/S0909049506049296 ]

Dose dependence of radiation damage for protein crystals studied at various X-ray energies

N. Shimizu, K. Hirata, K. Hasegawa, G. Ueno and M. Yamamoto

Synopsis: A systematic study of radiation damage has been conducted for various X-ray energies at beamline BL41XU at SPring-8. The dependence of radiation damage on photon energy is discussed.

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 11-23  [ doi:10.1107/S0909049506049806 ]

Cryoradiolytic reduction of crystalline heme proteins: analysis by UV-Vis spectroscopy and X-ray crystallography

T. Beitlich, K. Kühnel, C. Schulze-Briese, R. L. Shoeman and I. Schlichting

Synopsis: The X-ray-induced photoreduction of three different crystalline heme proteins was followed on-line by UV-Vis absorption spectroscopy and X-ray crystallography.

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 24-33  [ doi:10.1107/S0909049506046589 ]

The many faces of radiation-induced changes

D. Borek, S. L. Ginell, M. Cymborowski, W. Minor and Z. Otwinowski

Synopsis: Various effects of X-ray exposure observed during diffraction from macromolecular crystals are described. Impact on the quality of diffraction data, experimental phasing procedures and interpretation of biological results are analyzed.

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 34-42  [ doi:10.1107/S0909049506038970 ]

Modelling and refining site-specific radiation damage in SAD/MAD phasing

M. Schiltz and G. Bricogne

Synopsis: Site-specific radiation-induced phasing through dose-dependent modelling of heavy-atom parameters and the use of unmerged data is discussed.

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 43-50  [ doi:10.1107/S0909049506041045 ]

A comparison of SAD and two-wavelength MAD phasing for radiation-damaged Se-MET crystals

A. González

Synopsis: A comparison of SAD and two-wavelength MAD phasing under similar experimental conditions is made for three different seleno-methionine samples, and the implications for data collection strategy are discussed.

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 51-72  [ doi:10.1107/S0909049506048898 ]

XANES measurements of the rate of radiation damage to selenomethionine side chains

J. M. Holton

Synopsis: The lifetime of selenomethionine in a protein crystallography X-ray beam was measured and the effects of varying sample parameters such as temperature, volume, physical state, Se concentration, chemical additives and protein structure were evaluated. Depending on the combination of these parameters, the dose required to damage half of the selenomethionine in a sample varied by as much as a factor of eight.

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 73-83  [ doi:10.1107/S0909049506044177 ]

Radioprotectant screening for cryocrystallography

R. J. Southworth-Davies and E. F. Garman

Synopsis: A range of radioprotectants have been tested as a possible means of reducing radiation damage in protein cryocrystallography, using an online microspectrophotometer.

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 84-91  [ doi:10.1107/S0909049506038623 ]

Specific radiation damage to acidic residues and its relation to their chemical and structural environment

E. Fioravanti, F. M. D. Vellieux, P. Amara, D. Madern and M. Weik

Synopsis: The radiation susceptibilities of the acidic side-chains are analysed with respect to their chemical and structural environment in crystals of the malate dehydrogenase from Haloarcula marismortui.

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 92-98  [ doi:10.1107/S0909049506051259 ]

Tracking X-ray-derived redox changes in crystals of a methylamine dehydrogenase/amicyanin complex using single-crystal UV/Vis microspectrophotometry

A. R. Pearson, R. Pahl, E. G. Kovaleva, V. L. Davidson and C. M. Wilmot

Synopsis: A general multi-crystal macromolecular X-ray data collection strategy has been developed for dealing with rapid X-ray-induced redox changes, in which chromophore anisotropy in the crystal does not enable concurrent UV/Vis absorbance measurements during X-ray data collection. The use of this approach is demonstrated for reaction intermediates in the methylamine dehydrogenase oxidative half-reaction.

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 99-108  [ doi:10.1107/S0909049506043251 ]

X-ray radiation-induced damage in DNA monitored by online Raman

J. E. McGeehan, P. Carpentier, A. Royant, D. Bourgeois and R. B. G. Ravelli

Synopsis: Raman spectra have been collected from cryocooled DNA crystals in combination with macromolecular cystallography data collection. The specific X-ray-induced breakage of a C-Br bond could be monitored in detail by both techniques.

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 109-115  [ doi:10.1107/S090904950604605X ]

Non-invasive measurement of X-ray beam heating on a surrogate crystal sample

E. H. Snell, H. D. Bellamy, G. Rosenbaum and M. J. van der Woerd

Synopsis: Thermal imaging is used for the first time to measure the temperature rise of a sample exposed to a third-generation synchrotron X-ray beam.

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 116-127  [ doi:10.1107/S0909049506052307 ]

Radiation damage to protein specimens from electron beam imaging and diffraction: a mini-review of anti-damage approaches, with special reference to synchrotron X-ray crystallography

W. H. Massover

Synopsis: Modern approaches to limit the damaging effects of electron beam irradiation during diffraction and imaging for high-resolution polypeptide structure determination are reviewed. The important question of whether analogous means can be used to decrease radiation damage to protein crystals during synchrotron X-ray diffraction is critically discussed.

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 128-132  [ doi:10.1107/S0909049506043111 ]

Plastic-embedded protein crystals

R. B. G. Ravelli, U. Haselmann-Weiss, J. E. McGeehan, A. A. McCarthy, J. A. Marquez, C. Antony, A. S. Frangakis and G. Stranzl

Synopsis: The technique of freeze-substitution, commonly used in electron microscopy, has been applied to vitrified protein crystals. These plastic-embedded crystals have been examined in terms of X-ray diffraction properties and radiation hardness.

Online 15 December 2006


research papers


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J. Synchrotron Rad. (2007). 14, 133-137  [ doi:10.1107/S0909049506041835 ]

On the design of ultrafast shutters for time-resolved synchrotron experiments

M. Gembicky and P. Coppens

Synopsis: A comprehensive treatment of the limitations and possibilities for single-pulse selection in synchrotron operating modes with ~150 ns bunch separation, as occurs in the standard operating mode at the Advanced Photon Source, is presented.

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 138-143  [ doi:10.1107/S0909049506050205 ]

A sagittally focusing double-multilayer monochromator for ultrafast X-ray imaging applications

Y. Wang, S. Narayanan, J. Liu, D. Shu, A. Mashayekhi, J. Qian and J. Wang

Synopsis: The development of a sagittally focusing double-multilayer monochromator for X-ray radiographic and tomographic applications is described.

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 144-150  [ doi:10.1107/S0909049506043585 ]

A 3 × 6 arrayed CCD X-ray detector for continuous rotation method in macromolecular crystallography

K. Ito, Y. Gaponov, N. Sakabe and Y. Amemiya

Synopsis: A 3 × 6 arrayed charge-coupled device (CCD) X-ray detector has been developed for the continuous-rotation method in macromolecular crystallography at the Photon Factory.

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 151-157  [ doi:10.1107/S0909049506048783 ]

Application of a hybrid pixel detector to powder diffraction

S. Basolo, J.-F. Bérar, N. Boudet, P. Breugnon, B. Caillot, J.-C. Clemens, P. Delpierre, B. Dinkespiler, S. Hustache, I. Koudobine, C. Meessen, M. Menouni, C. Mouget, H. Palancher, P. Pangaud, R. Potheau and E. Vigeolas

Synopsis: Use of pixel detectors at synchrotron beams.

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 158-162  [ doi:10.1107/S090904950604684X ]

Preliminary quantitative analysis of myocardial fatty acid metabolism from fluorescent X-ray computed tomography imaging

Thet-Thet-Lwin, T. Takeda, J. Wu, N. Sunaguchi, T. Murakami, S. Mouri, S. Nasukawa, Q. Huo, T. Yuasa, K. Hyodo and T. Akatsuka

Synopsis: An introduction to fluorescent X-ray computed tomography imaging with synchrotron radiation to detect fatty acid metabolic abnormalities in cardiomyopathy.

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 163-168  [ doi:10.1107/S0909049506045304 ]

An off-axis magnetron-sputtering system for in situ studies of artificial superlattices growth by synchrotron radiation scattering

Y.-C. Liang, H.-Y. Lee, H.-J. Liu, Y.-W. Hsieh and Y.-C. Liang

Synopsis: The design of an off-axis magnetron sputtering chamber for in situ X-ray scattering study of superlattices film growth is described. The performance of the chamber is demonstrated by observation of the effects of the modulation length and the stacking period on the growth characteristics of BaTiO3/LaNiO3 artificial superlattices.

Online 15 December 2006


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J. Synchrotron Rad. (2007). 14, 169-172  [ doi:10.1107/S0909049506049971 ]

Verifying DiffEXAFS measurements with differential X-ray diffraction

M. P. Ruffoni, R. F. Pettifer, S. Pascarelli and O. Mathon

Synopsis: Differential EXAFS (DiffEXAFS) is a novel technique for measuring atomic perturbations on a local scale. Here a complementary technique for such studies is presented: differential X-ray diffraction (DiffXRD), which may be used to independently verify DiffEXAFS results whilst using exactly the same experimental apparatus and measurement technique. A test experiment has been conducted to show that DiffXRD can be used to successfully determine the thermal expansion coefficient of SrF2.

Online 15 December 2006


current events


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J. Synchrotron Rad. (2007). 14, 173-174  [ doi:10.1107/S0909049506053003 ]

Current events

Online 15 December 2006


international union of crystallography


 

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J. Synchrotron Rad. (2007). 14, 175-179  [ doi:10.1107/S0909049506044967 ]

Notes for authors 2007

Online 15 December 2006


 

J. Synchrotron Rad. (2007). 14, 180

forthcoming meetings and short courses


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