Figure 2
On-line microspectrophotometric analysis of HEWL crystallization buffer and its components at RT. (a) A difference absorption spectrum (spectra after irradiation - spectra before irradiation) of the HEWL buffer constituents in water (purple: 1.4 M NaCl at 4.25 MGy; green: 0.1 M NaAc at 2.59 MGy; blue: both at 4.26 MGy). (b) A stacked absorbance spectrum of 30% glycerol upon irradiation, to a final dose of 0.27 MGy as indicated by the red plot. (c) A stacked absorbance spectrum of 30% glycerol and 0.1 M sodium acetate pH 4.8, upon irradiation, to a final dose of 0.3 MGy as indicated by the red plot. (d) A stacked absorbance spectrum of 30% glycerol and 1.4 M sodium chloride upon irradiation, to a final dose of 0.14 MGy as indicated by the red plot. (e) A stacked absorbance spectrum of the crystallization buffer (30% glycerol, 0.1 M sodium acetate pH 4.8, 1.4 M sodium chloride) upon irradiation, to a final dose of 0.39 as indicated by the red plot.  [article HTML]