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Figure 2
UV/Vis absorption spectrum of a plate-like crystal (50 µm × 20 µm × 10 µm) of partially reduced horse heart myoglobin. To minimize morphological artifacts, the crystal was rotated along ω to yield best absorption spectra. Acquisition time was 100 × 2.6 ms with an entrance slit width of 100 µm, using a 300 lines mm−1 grating and a UV-optimized EMCCD detector. The wavelength range in the UV is accessible down to at least 250 nm.

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