Journal of Synchrotron Radiation

Volume 20, Part 5 (September 2013)

short communications

J. Synchrotron Rad. (2013). 20, 805-808    [ doi:10.1107/S0909049513020372 ]

Acoustic methods for high-throughput protein crystal mounting at next-generation macromolecular crystallographic beamlines

C. G. Roessler, A. Kuczewski, R. Stearns, R. Ellson, J. Olechno, A. M. Orville, M. Allaire, A. S. Soares and A. Héroux

Abstract: To take full advantage of advanced data collection techniques and high beam flux at next-generation macromolecular crystallography beamlines, rapid and reliable methods will be needed to mount and align many samples per second. One approach is to use an acoustic ejector to eject crystal-containing droplets onto a solid X-ray transparent surface, which can then be positioned and rotated for data collection. Proof-of-concept experiments were conducted at the National Synchrotron Light Source on thermolysin crystals acoustically ejected onto a polyimide `conveyor belt'. Small wedges of data were collected on each crystal, and a complete dataset was assembled from a well diffracting subset of these crystals. Future developments and implementation will focus on achieving ejection and translation of single droplets at a rate of over one hundred per second.

Keywords: acoustic droplet ejection; conveyor belt; crystal mounting; high throughput; X-ray diffraction; macromolecular crystallography.

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[ doi:10.1107/S0909049513020372/wa5056sup1.pdf ]
Statistics for all the thermolysin datasets collected using the acoustic injection method and the conveyor belt at beamline X25


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