Figure 4
SDS-PAGE of the incubation mixture of 1510-N [Delta]126-133 and 1511-C. 1510-N [Delta]126-133 (1 µg) was mixed with the substrate 1511-C (2.5 µg) in 20 mM MES-NaOH (pH 6.0), and incubated at 353 K for 0, 10, 30, 60, 120 and 240 min. Products degraded by 1510-N [Delta]126-133 were not detected. Lane M indicates molecular markers.  [article HTML]