Comparison of the interface waters of two SPNct–IFS complex structures. Left: the 1.70 Å structure reported in this study (PDB entry 4kt6
). Right: 2.80 Å structure reported previously by Smith et al. (2011) (PDB entry 3pnt
). The A:B interface of the SPNct–IFS complex is shown, representing the SPN subunits as a molecular surface and the IFS subunits as a ribbon. The interfaces are highlighted in cyan and yellow, respectively. Red spheres are the water molecules present at the interface. The interface waters were assigned using the program AquaProt (Reichmann et al., 2007). Structural figures were drawn using PyMOL (DeLano, 2002).