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Figure 3
Demonstration of the ability of DPC X-ray images to provide high-accuracy alignment of low-dose X-ray fluorescence images. A freeze-dried oocyte was imaged at APS beamline 2-ID-E using `fly scans' with per-pixel times of 3 ms (images on the left) and 30 ms (images on the right), and both zinc fluorescence images (top) and diagonal DPC images (bottom) were recorded simultaneously. The lack of detail and low signal in the zinc fluorescence projections precludes precise alignment (broad peaks with spurious sub-peaks in cross correlation images B and D), while the diagonal DPC images deliver sharp and low-noise cross-correlation peaks even at 10× reduced X-ray dose (image F).

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