Journal of Synchrotron Radiation

Volume 21, Part 5 (September 2014)

research papers

J. Synchrotron Rad. (2014). 21, 1175-1179    [ doi:10.1107/S1600577514014611 ]

Three-dimensional study of poly(lactic co-glycolic acid) micro-porous microspheres using hard X-ray nano-tomography

D. Wang, N. Li, Z. Wang, K. Gao, Y. Zhang, Y. Luo, S. Wang, Y. Bao, Q. Shao and Z. Wu

Abstract: Poly(lactic co-glycolic acid) (PLGA) is widely used in diverse fields, especially in delivering biologically active proteins and drugs. For these applications, the knowledge of morphology and microstructure of PLGA micro-porous microspheres is of great importance since they strongly influence the drug delivering efficiency. In this study, micro-porous PLGA microspheres loaded by bovine serum albumin are investigated by using a full-field Zernike phase contrast transmission hard X-ray microscope. From three-dimensional reconstructions and segmentations, fundamental microstructural parameters such as size, shape, distribution and volume ratio among pores and proteins inside PLGA microspheres were obtained. These parameters are useful to understand the relationship between the internal microstructure and drug encapsulation, as well as the drug release efficiency of PLGA microspheres. The presented results demonstrate the capability of hard X-ray nano-tomography to characterize porous microspheres loaded with proteins and drugs, and also open a way to analyse, optimize and design new PLGA microspheres for specific applications.

Keywords: hard X-ray imaging; nano-tomography; PLGA microspheres; salt concentration; drug delivery.

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Moving Picture Experts Group (MPG) video file (32490.1 kbytes)
[ doi:10.1107/S1600577514014611/hf5256sup1.mpg ]
Video 1. Microsphere 1 pore slice movie

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Moving Picture Experts Group (MPG) video file (31719.4 kbytes)
[ doi:10.1107/S1600577514014611/hf5256sup2.mpg ]
Video 2. Microsphere 2 pore slice movie

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Moving Picture Experts Group (MPG) video file (37395.5 kbytes)
[ doi:10.1107/S1600577514014611/hf5256sup3.mpg ]
Video 3. Microsphere 3 pore slice movie


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