 | Acta Crystallographica Section B Acta Crystallographica Section B STRUCTURAL SCIENCE, CRYSTAL ENGINEERING AND MATERIALS |
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![[Figure 10]](eb5049fig10mag.jpg)
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Figure 10
(a) Absorption spectrum in buffer (pH 2):ethanol (1:1) of the `closed' (black, 301 nm) and `open' (red, 531 nm) isomer for (II). In blue, the fluorescence emission spectra of the `open' form (551 nm) (the `closed' one does not have fluorescence emission). (b) Fluorescence signal of a single crystal of compound (II) measured in a wide-field microscope during the course of the photoisomerization induced with UV light (355 nm). The excitation laser (532 nm) was on during the whole experiment and the UV laser (355 nm) was switched on after 40 s; detection was selected with a band-pass filter of 580/40 nm. The acquisition frequency (frame rate) was 0.1 s, and the intensity of the sources was 1 mW (355 nm) and 120 mW (532 nm), measured at the back focal plane of the objective lens. In the inset, the last frame (wide-field image of the crystal) of the series is shown. A dotted square shows the area where the signal was integrated over time. |
![[Figure 10]](eb5049fig10.jpg)
| STRUCTURAL SCIENCE CRYSTAL ENGINEERING MATERIALS |
ISSN: 2052-5206
