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Figure 1
SDS–PAGE of PAI-1-stab. Lanes 1 and 2, PAI-1-stab before crystallization; lanes 3 and 4, PAI-1-stab after crystallization. PAI-1-stab was subjected to SDS–PAGE as such (lanes 1 and 3) or after incubation with a twofold molar excess of t-PA (lanes 2 and 4). PAI-1-stab was analyzed at a concentration of 100–250 µg ml−1. The arrow indicates the migration position of the PAI-1-stab–t-PA complex, originating from the active form of PAI-1-stab. The open and closed arrow head indicate the migration position of intact and cleaved PAI-1-stab, respectively. |