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Figure 8
Anomalous Fourier difference-maps obtained with data set #3. The transferase enzyme was soaked with thiomersalate. Data were collected at 0.931 Å. Maps were calculated using the program FFT (Collaborative Computational Project Number 4, 1994BB4). (a) Map obtained after processing with DENZO, (b) map obtained after processing with PrOW plus `q weighting'. The maps are shown at a contour level corresponding to the peak value of the anomalous peak (11.6σ for the first map, 13.0σ for the second) divided by 4.9. In these conditions, the displayed volumes of the anomalous peaks are roughly the same in the two maps. Anomalous peaks do not fall exactly on top of the Hg atom because model refinement was performed with an independent data set and did not take into account anomalous signals.

Journal logoBIOLOGICAL
ISSN: 1399-0047
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