 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 3]](ba0030fig3mag.jpg)
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Figure 3
Portions of three adjacent images of 0.25° rotation each: (a) 0.00–0.25°, (b) 0.25–0.50°, (c) 0.50–0.75° and (d) the superposition of these three images. Blue ellipses are centered on the observed peaks in (a)–(c) and superimposed in (d). This figure illustrates several features of thin-sliced (0.25°) images versus thick-sliced (0.75°) images. Firstly, thin-sliced data reduces spatial overlaps: the unoverlapped reflections at the top of (a) and (c) are overlapped in (d). Secondly, thin-sliced data reduces saturation: the strong reflection near the center of (a)–(c) sums to be saturated in (d). Thirdly, true spot centroids require the complete reflection in three dimensions or fully recorded reflections in two dimensions: the reflection centroids of the partially recorded reflections shift slightly as the crystal is rotated, as shown by the non-superposition of ellipses in (d). The streaking of the spots arises from the mirror optics used. |
![[Figure 3]](ba0030fig3.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
