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Figure 1
Visualization of different crystals with the current microfocus optics. In (a), the 20 × 10 × 50 µm crystal looks decently sized; in (b), the thickness of the needle is clearly less than 5 µm; in (c) and (d), the largest dimension of these crystals does not exceed 50 µm; (e) and (f) demonstrate the visualization of plates thinner than 2–3 µm, while in (g) and (h) the path of the beam (the darker area) when shooting a crystal in one direction (g, dark stripe) or two perpendicular directions (h, dark stripe and darker spot) can be observed. The darker colour (blue in the colour camera) has often been observed in cryo-protectants containing polyethylene glycol.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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