Figure 1
GL-based ab initio phasing. tRNAAsp–Asp RS complex, with full permutation for the 12 strongest reflections (d > 68 Å), generating 4096 phase sets. For the GL calculation at d = 60 Å, the parameters were B = 8000, V = 0.3 of the cell, N = 100 atoms, ω = 0.82. The triangle corresponds to the variant closest to the correct solution. Note that the Cφ value is very close but not equal to 1.0 owing to the inclusion of the acentric reflections in permutation. |