Figure 3
GL-based ab initio phasing of γ-crystallin IIIb. The phases of four strong reflections were fixed and the phases of nine reflections (d > 29 Å) were permuted. For GL calculation at d = 23 Å, the parameters were B = 100, V = 0.4 of the cell, N = 100 pseudo-atoms, ω = 0.80. The triangle corresponds to the correct solution, which is shown for comparison with all the permuted variants. |