Figure 7
(a) Stereo diagram of a portion of the HRV16-type 2 ICAM-1 cryo-EM reconstruction corresponding to the density (light blue) for the two-domain ICAM-1 fragment. Superimposed in yellow is the difference density map between HRV16-type 1 (fully glycosylated) and HRV16-type 2 (mostly deglycosylated) reconstructions. The density in the HRV16-type 2 reconstruction has been suitably scaled to account for the lower occupancy of the ICAM-1 fragments. (b) Fitting of the refined type 1 ICAM-1 model into the cryo-EM reconstruction of the HRV16-type 1 ICAM-1 reconstruction. The protein is represented in white and the disordered carbohydrates are represented by an ensemble of conformations (yellow). [Reprinted by permission from Kolatkar et al. (1999), EMBO J. 18, 6249–6259. Copyright (1999) European Molecular Biology Organization.] |