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Figure 11
Refinement by projection matching. A 3D map is projected into a set of different orientations to create reference images. Each raw image in the data set is translationally and rotationally aligned to each reference image in turn and is assigned the orientation of the one giving the highest correlation coefficient. The aligned images are grouped and averaged and these averages are used to create an improved 3D map. The procedure is iterated until the maps converge. Reprojections of the maps are compared to the preceding set of class averages to check for consistency.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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